Reviewed, UniProtKB/Swiss-Prot Q3Z6Z8 (RNPH_DEHE1)

Names and origin

Protein names

Recommended name:
    Ribonuclease PH
      Short name=RNase PH
    EC=2.7.7.56
Alternative name(s):
    tRNA nucleotidyltransferase

Gene names

Name:	rph
Ordered Locus Names:	DET1289

Organism

Dehalococcoides ethenogenes (strain 195) [Complete proteome] [HAMAP]

Taxonomic identifier

243164 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Dehalococcoidetes › Dehalococcoides

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity.
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribonuclease PH HAMAP MF_00564		PRO_1000061131

Sequences

	Sequence		Length	Mass (Da)
	Q3Z6Z8-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 144941419CF0BB1E	FASTA	239	26,385
	10 20 30 40 50 60 MQRGDGRNFN QLRPITITPG FQSFAEGSVL IEQGKTRVIC SVSMEDKVPQ FLRNSGTGWV 70 80 90 100 110 120 TAEYSMLPRS TVTRTQRDSS AGKISGRSQE IQRLIGRSLR SCVDLAALGE RSFIIDCDVI 130 140 150 160 170 180 QADAGTRTAS ITGAYIALYL AFKKMVDMGI LSKMPFTSQV AAVSVSIFKG NIVLDPCYDE 190 200 210 220 230 DFQAEVDFNL VMNDRAEFVE IQGTAEGKTF SRDTLDQVLK LGEAGIWQLF DIQNSITRP

References

[1]

"Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes." Seshadri R., Adrian L., Fouts D.E., Eisen J.A., Phillippy A.M., Methe B.A., Ward N.L., Nelson W.C., DeBoy R.T., Khouri H.M., Kolonay J.F., Dodson R.J., Daugherty S.C., Brinkac L.M., Sullivan S.A., Madupu R., Nelson K.E., Kang K.H. , Impraim M., Tran K., Robinson J.M., Forberger H.A., Fraser C.M., Zinder S.H., Heidelberg J.F. Science 307:105-108(2005) [PubMed: 15637277] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000027 Genomic DNA. Translation: AAW39419.1.
RefSeq	YP_182001.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3229379.
GenomeReviews	Gene locus DET1289 in contig CP000027_GR.
KEGG	det:DET1289.
NMPDR	fig|243164.3.peg.1282.
TIGR	DET1289.
Phylogenomic databases
HOGENOM	Q3Z6Z8.
OMA	Q3Z6Z8. YAMLPRA.
Enzyme and pathway databases
BioCyc	DETH243164:DET_1289-MON.
Family and domain databases
HAMAP	MF_00564. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR018336. Ribonuclease-PH_CS. IPR002381. RNase_PH_bac-type. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_DEHE1

Accession

Primary (citable) accession number: Q3Z6Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 27, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)