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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi108 – 1081ZincUniRule annotation
Metal bindingi110 – 1101ZincUniRule annotation
Metal bindingi135 – 1351ZincUniRule annotation
Metal bindingi137 – 1371ZincUniRule annotation
Binding sitei248 – 2481ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDETH243164:GJNF-1366-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:DET1365
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
ProteomesiUP000008289 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484Glutamate--tRNA ligasePRO_0000237357Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243164.DET1365.

Structurei

3D structure databases

ProteinModelPortaliQ3Z6S3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi245 – 2495"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiNSSPSCF.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3Z6S3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNEVRVRYA PSPTGYPHLG NIRTAMFNWL FARHNGGKFI VRIEDTDRER
60 70 80 90 100
YVEGAVESIL ESLNWLGLDW DEGPDKGGDY GPYYQSERLF LYRQAAERLV
110 120 130 140 150
TEGKAYYCHC SSERLDKMRE EQIARKEPPG YDRCCRDLCL GQKEGAVIRF
160 170 180 190 200
KIPLEGQTTF IDLIRGEVTF DNAKQDDFVI LKSDGFPTYH LASVVDDHAM
210 220 230 240 250
QISHVLRAEE WLPSTPKHLM LYKALGYTPP QYAHLPMILG PDRSKLSKRH
260 270 280 290 300
GATSTIEYKQ AGYLPETMVN FLSLLGWAYD DKTELFSRKQ LIEYFCLEKV
310 320 330 340 350
SKTAAIFNYE KLDWMNGMYI RTLSAPDLAS RAIPFLEKDE RIAASGRLNF
360 370 380 390 400
DYTTKVMPLI QERVKKLSEL AELCWFIYSD NISYDPASLI DKKLTKDESL
410 420 430 440 450
CALKAAFARL EALANFDAVS MEEHIRPLAA ELELKPGQLF GMLRTASTGQ
460 470 480
QVAPPLFQTM EVLGRERCLG RIAMAIARLS ELPS
Length:484
Mass (Da):55,256
Last modified:September 27, 2005 - v1
Checksum:i43DA9EFFDA675ADA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA. Translation: AAW39383.1.
RefSeqiWP_010937053.1. NC_002936.3.

Genome annotation databases

EnsemblBacteriaiAAW39383; AAW39383; DET1365.
GeneIDi3229342.
KEGGidet:DET1365.
PATRICi21609757. VBIDehEth89364_1293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA. Translation: AAW39383.1.
RefSeqiWP_010937053.1. NC_002936.3.

3D structure databases

ProteinModelPortaliQ3Z6S3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243164.DET1365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAW39383; AAW39383; DET1365.
GeneIDi3229342.
KEGGidet:DET1365.
PATRICi21609757. VBIDehEth89364_1293.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiNSSPSCF.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciDETH243164:GJNF-1366-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-2266 / KCTC 15142 / 195.

Entry informationi

Entry nameiSYE_DEHM1
AccessioniPrimary (citable) accession number: Q3Z6S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 27, 2005
Last modified: July 22, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.