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Protein

Anthranilate synthase component 1

Gene

trpE

Organism
Dehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.UniRule annotation

Catalytic activityi

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD-1), Anthranilate phosphoribosyltransferase (trpD)
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.

Names & Taxonomyi

Protein namesi
Recommended name:
Anthranilate synthase component 1UniRule annotation (EC:4.1.3.27UniRule annotation)
Gene namesi
Name:trpEUniRule annotationImported
Ordered Locus Names:DET1481Imported
OrganismiDehalococcoides mccartyi (strain ATCC BAA-2266 / KCTC 15142 / 195) (Dehalococcoides ethenogenes (strain 195))Imported
Taxonomic identifieri243164 [NCBI]
Taxonomic lineageiBacteriaChloroflexiDehalococcoidiaDehalococcoidalesDehalococcoidaceaeDehalococcoides
Proteomesi
  • UP000008289 Componenti: Chromosome

Interactioni

Subunit structurei

Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).UniRule annotation

Protein-protein interaction databases

STRINGi243164.DET1481.

Structurei

3D structure databases

ProteinModelPortaliQ3Z6G8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 153Anth_synt_I_NInterPro annotationAdd BLAST126
Domaini208 – 461Chorismate_bindInterPro annotationAdd BLAST254

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili452 – 479Sequence analysisAdd BLAST28

Sequence similaritiesi

Belongs to the anthranilate synthase component I family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiENOG4105CRQ. Bacteria.
COG0147. LUCA.
HOGENOMiHOG000025142.
KOiK01657.
OMAiIAGTFKR.
OrthoDBiPOG091H01VQ.

Family and domain databases

Gene3Di3.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR005801. ADC_synthase.
IPR019999. Anth_synth_I-like.
IPR006805. Anth_synth_I_N.
IPR005256. Anth_synth_I_PabB.
IPR015890. Chorismate_C.
PfamiView protein in Pfam
PF04715. Anth_synt_I_N. 1 hit.
PF00425. Chorismate_bind. 1 hit.
PRINTSiPR00095. ANTSNTHASEI.
SUPFAMiSSF56322. SSF56322. 1 hit.
TIGRFAMsiTIGR00564. trpE_most. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3Z6G8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYYPSLAEVK KLAAQGNLIP ISCEIMADLE TPVSAFLKIK DSQNAFLLES
60 70 80 90 100
VEGGERVARY SFIGTNPHKV LTAYQTDTVP PLTQVENELN KYRVVPVGDL
110 120 130 140 150
PRFCGGAVGF LGYEAVTRFE ELPSPSADPL NLPEAVFMLV DTMLVFDHIS
160 170 180 190 200
HSIKVLSYVH TEQDIETSYN QAIRNIENLV NRLRKPLPET APKSTAASIP
210 220 230 240 250
EMKSNFKQAD FEGKVSKIRD YLNSGEAIQV VLSQRLSRPT SAHPFDIYRA
260 270 280 290 300
LRSVNPSPYM YYLDFGDFQI VGASPEVLVR VEDGEVMTRP LAGTRKRGKT
310 320 330 340 350
QKEDTSLEQE LRHDEKECAE HIMLVDLGRN DIGRISQPGT VRITDVMDVE
360 370 380 390 400
RYSHVMHLVS HVQGKLKPNI TPFEALQSCF PAGTVSGAPK IRAMEIIAEM
410 420 430 440 450
ETEKRGIYAG AVGYFSYSGN MDMAIAIRTM VVKGGIAHIQ AGCGIVSDSV
460 470 480
PEHEYQETLN KAQALLKALD RAENQASEKP HVITN
Length:485
Mass (Da):53,787
Last modified:September 27, 2005 - v1
Checksum:iDBE77B5A5B1E6640
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000027 Genomic DNA. Translation: AAW39331.1.
RefSeqiWP_010937158.1. NC_002936.3.

Genome annotation databases

EnsemblBacteriaiAAW39331; AAW39331; DET1481.
KEGGidet:DET1481.
PATRICifig|243164.10.peg.1397.

Similar proteinsi

Entry informationi

Entry nameiQ3Z6G8_DEHM1
AccessioniPrimary (citable) accession number: Q3Z6G8
Entry historyiIntegrated into UniProtKB/TrEMBL: September 27, 2005
Last sequence update: September 27, 2005
Last modified: July 5, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported