Reviewed, UniProtKB/Swiss-Prot Q3Z586 (MHPB_SHISS)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	SSON_0295

Organism

Shigella sonnei (strain Ss046) [Complete proteome] [HAMAP]

Taxonomic identifier

300269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653
Cofactor	Fe2+ ion By similarity. HAMAP MF_01653
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653
Subunit structure	Homotetramer By similarity. HAMAP MF_01653
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653		PRO_0000337669
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3Z586-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: E1D5A8574E5DFE05	FASTA	314	34,196
	10 20 30 40 50 60 MHAYLHCLSH SPLVGYVDPA QEVLDEVNGV IASARERIAA FSPELVVLFA PDHYNGFFYD 70 80 90 100 110 120 VMPPFCLGVG ATAIGDFGSA AGELPVPVEL AEACAHAVMK SGIDLAVSYC MQVDHGFAQP 130 140 150 160 170 180 LEFLLGGLDK VPVLPVFING VATPLPGFQR TRMLGEAIGR FTSTLNKRVL FLGSGGLSHQ 190 200 210 220 230 240 PPVPELAKAD AHMRDRLLGS GKDLPASERE LRQQRVISAA EKFVEDQRTL HPLNPIWDNQ 250 260 270 280 290 300 FMTLLEQGRI QELDAVSNEE LSAIAGKSTH EIKTWVAAFA AISAFGNWRS EGRYYRPIPE 310 WIAGFGSLSA RTEN

References

[1]

"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q. Nucleic Acids Res. 33:6445-6458(2005) [PubMed: 16275786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000038 Genomic DNA. Translation: AAZ87076.1.
RefSeq	YP_309311.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3Z586.
Genome annotation databases
GeneID	3669105.
GenomeReviews	Gene locus SSON_0295 in contig CP000038_GR.
KEGG	ssn:SSON_0295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG42398.
HOGENOM	HBG565586.
OMA	PLNPEWD.
Enzyme and pathway databases
BioCyc	SSON300269:SSO_0295-MONOMER.
Family and domain databases
HAMAP	MF_01653. MhpB. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_SHISS

Accession

Primary (citable) accession number: Q3Z586

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 27, 2005
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)