Q3Z554 (HOA_SHISS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-2-oxovalerate aldolase
Short name=HOA
EC=4.1.3.39

Alternative name(s):
4-hydroxy-2-keto-pentanoic acid aldolase
4-hydroxy-2-oxopentanoate aldolase

Gene names

Name:	mhpE
Ordered Locus Names:	SSON_0331

Organism

Shigella sonnei (strain Ss046) [Complete proteome] [HAMAP]

Taxonomic identifier

300269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the retro-aldol cleavage of 4-hydroxy-2-oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds By similarity. HAMAP MF_01656
Catalytic activity	4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate. HAMAP MF_01656
Pathway	Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01656
Subunit structure	Interacts with mhpF By similarity. HAMAP MF_01656
Sequence similarities	Belongs to the 4-hydroxy-2-oxovalerate aldolase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4-hydroxy-2-oxovalerate aldolase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	4-hydroxy-2-oxovalerate aldolase HAMAP MF_01656		PRO_0000337808
Regions
	Region	14 – 15	2	Substrate binding By similarity
Sites
	Active site	18	1	Proton acceptor Potential
	Metal binding	15	1	Manganese By similarity
	Metal binding	197	1	Manganese; via tele nitrogen By similarity
	Metal binding	199	1	Manganese; via tele nitrogen By similarity
	Binding site	168	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Binding site	288	1	Substrate By similarity
	Site	14	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3Z554 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: A2B4F6A1598DB91A	FASTA	337	36,502
	10 20 30 40 50 60 MNGKKLYISD VTLRDGMHAI RHQYSLENVR QIAKALDDAR VDSIEVAHGD GLQGSSFNYG 70 80 90 100 110 120 FGAHSDLEWI EAAADVVRHA KIATLLLPGI GTIHDLKNAW QAGARVVRVA THCTEADVSA 130 140 150 160 170 180 QHIQYARELG MDTVGFLMMS HMTTPENLAK QAKLMEGYGA TCIYVVDSGG AMNMSDIRDR 190 200 210 220 230 240 FRALKAVLKP ETQTGMHAHH NLSLGVANSI AAVEEGCDRI DASFAGMGAG AGNAPLEVFI 250 260 270 280 290 300 AAADKLGWQH GTDLYALMDA ADDLVRPLQD RPVRVDRETL ALGYAGVYSS FLRHCETAAA 310 320 330 RYGLSAVDIL VELGKRRMVG GQEDMIVDVA LDLRNNK

References

[1]

"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q. Nucleic Acids Res. 33:6445-6458(2005) [PubMed: 16275786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Ss046.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000038 Genomic DNA. Translation: AAZ87108.1.
RefSeq	YP_309343.1. NC_007384.1.
3D structure databases
ProteinModelPortal	Q3Z554.
SMR	Q3Z554. Positions 4-333.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3Z554.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000099547; EBESCP00000095576; EBESCG00000098591.
GeneID	3668930.
GenomeReviews	Gene locus SSON_0331 in contig CP000038_GR.
KEGG	ssn:SSON_0331.
PATRIC	18734012. VBIShiSon107113_0379.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0119.
GeneTree	EBGT00050000011169.
HOGENOM	HBG300122.
OMA	ATCVYVV.
ProtClustDB	PRK08195.
Enzyme and pathway databases
BioCyc	SSON300269:SSO_0331-MONOMER.
Family and domain databases
HAMAP	MF_01656. HOA. [Tree]
InterPro	IPR017629. 4OH_2_O-val_aldolase. IPR013785. Aldolase_TIM. IPR012425. DmpG_comm. IPR000891. PYR_CT. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01666.
PANTHER	PTHR10277:SF3. PTHR10277:SF3. 1 hit.
Pfam	PF07836. DmpG_comm. 1 hit. PF00682. HMGL-like. 1 hit. [Graphical view]
ProDom	PD005364. DmpG_comm. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03217. 4OH_2_O_val_ald. 1 hit.
PROSITE	PS50991. PYR_CT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOA_SHISS

Accession

Primary (citable) accession number: Q3Z554

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program