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Q3Z3T2

- RIMK_SHISS

UniProt

Q3Z3T2 - RIMK_SHISS

Protein

Ribosomal protein S6--L-glutamate ligase

Gene

rimK

Organism
Shigella sonnei (strain Ss046)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Is an L-glutamate ligase that catalyzes the ATP-dependent post-translational addition of glutamate residues to the C-terminus of ribosomal protein S6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei141 – 1411ATPUniRule annotation
    Binding sitei187 – 1871ATPUniRule annotation
    Metal bindingi248 – 2481Magnesium or manganese 1UniRule annotation
    Metal bindingi260 – 2601Magnesium or manganese 1UniRule annotation
    Metal bindingi260 – 2601Magnesium or manganese 2UniRule annotation
    Metal bindingi262 – 2621Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi178 – 1792ATPUniRule annotation
    Nucleotide bindingi211 – 2133ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. manganese ion binding Source: UniProtKB-HAMAP
    4. ribosomal S6-glutamic acid ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. C-terminal protein amino acid modification Source: UniProtKB-HAMAP
    2. translation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSSON300269:GJJF-834-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosomal protein S6--L-glutamate ligaseUniRule annotation (EC:6.3.2.-UniRule annotation)
    Alternative name(s):
    Poly-alpha-glutamate synthaseUniRule annotation
    Ribosomal protein S6 modification proteinUniRule annotation
    Gene namesi
    Name:rimKUniRule annotation
    Ordered Locus Names:SSON_0837
    OrganismiShigella sonnei (strain Ss046)
    Taxonomic identifieri300269 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
    ProteomesiUP000002529: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300Ribosomal protein S6--L-glutamate ligasePRO_0000205485Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi300269.SSON_0837.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3Z3T2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini104 – 287184ATP-graspUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RimK family.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0189.
    HOGENOMiHOG000293092.
    KOiK05844.
    OMAiCREPRLY.
    OrthoDBiEOG6DZDX8.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_01552. RimK.
    InterProiIPR011761. ATP-grasp.
    IPR013651. ATP-grasp_RimK-type.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR023533. Ribosomal_S6_modifier_RimK.
    IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
    [Graphical view]
    PfamiPF08443. RimK. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00768. rimK_fam. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3Z3T2-1 [UniParc]FASTAAdd to Basket

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    MKIAILSRDG TLYSCKRLRE AAIQRGHLVE ILDPLSCYMN INPAASSIHY    50
    KGRKLPHFDA VIPRIGTAIT FYGTAALRQF EMLGSYPLNE SVAIARARDK 100
    LRSMQLLARQ GIDLPVTGIA HSPDDTSDLI DMVGGAPLVV KLVEGTQGIG 150
    VVLAETRQAA ESVIDAFRGL NAHILVQEYI KEAQGCDIRC LVVGDEVVAA 200
    IERRAKEGDF RSNLHRGGAA SVASITPQER EIAIKAARTM ALDVAGVDIL 250
    RANRGPLVME VNASPGLEGI EKTTGIDIAG KMIRWIERHA TTEYCLKTGG 300
    Length:300
    Mass (Da):32,436
    Last modified:September 27, 2005 - v1
    Checksum:iC0AF021292ED41DD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000038 Genomic DNA. Translation: AAZ87580.1.
    RefSeqiYP_309815.1. NC_007384.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ87580; AAZ87580; SSON_0837.
    GeneIDi3666327.
    KEGGissn:SSON_0837.
    PATRICi18735228. VBIShiSon107113_0974.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000038 Genomic DNA. Translation: AAZ87580.1 .
    RefSeqi YP_309815.1. NC_007384.1.

    3D structure databases

    ProteinModelPortali Q3Z3T2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 300269.SSON_0837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ87580 ; AAZ87580 ; SSON_0837 .
    GeneIDi 3666327.
    KEGGi ssn:SSON_0837.
    PATRICi 18735228. VBIShiSon107113_0974.

    Phylogenomic databases

    eggNOGi COG0189.
    HOGENOMi HOG000293092.
    KOi K05844.
    OMAi CREPRLY.
    OrthoDBi EOG6DZDX8.

    Enzyme and pathway databases

    BioCyci SSON300269:GJJF-834-MONOMER.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_01552. RimK.
    InterProi IPR011761. ATP-grasp.
    IPR013651. ATP-grasp_RimK-type.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR023533. Ribosomal_S6_modifier_RimK.
    IPR004666. RpS6_RimK/Lys_biosynth_LsyX.
    [Graphical view ]
    Pfami PF08443. RimK. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00768. rimK_fam. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ss046.

    Entry informationi

    Entry nameiRIMK_SHISS
    AccessioniPrimary (citable) accession number: Q3Z3T2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3