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Q3Z1M2 (MAO1_SHISS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD-dependent malic enzyme

Short name=NAD-ME
EC=1.1.1.38
Gene names
Name:maeA
Ordered Locus Names:SSON_1645
OrganismShigella sonnei (strain Ss046) [Complete proteome] [HAMAP]
Taxonomic identifier300269 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP-Rule MF_01619

Oxaloacetate = pyruvate + CO2. HAMAP-Rule MF_01619

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01619

Sequence similarities

Belongs to the malic enzymes family.

Sequence caution

The sequence AAZ88340.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565NAD-dependent malic enzyme HAMAP-Rule MF_01619
PRO_0000323540

Sites

Active site1041Proton donor By similarity
Active site1751Proton acceptor By similarity
Metal binding2461Divalent metal cation By similarity
Metal binding2471Divalent metal cation By similarity
Metal binding2701Divalent metal cation By similarity
Binding site1571NAD By similarity
Binding site2701NAD By similarity
Binding site4181NAD By similarity
Site2701Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3Z1M2 [UniParc].

Last modified March 18, 2008. Version 2.
Checksum: EFD2C9A3F7028F4D

FASTA56563,132
        10         20         30         40         50         60 
MEPKTKKQRS LYIPYAGPVL LEFPLLNKGS AFSMEERRNF NLLGLLPEVV ETIEEQAERA 

        70         80         90        100        110        120 
WIQYQGFKTE IDKHIYLRNI QDTNETLFYR LVNNHLDEMM PVIYTPTVGA ACERFSEIYR 

       130        140        150        160        170        180 
RSRGVFISYQ NRHNMDDILQ NVPNHNIKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 

       190        200        210        220        230        240 
ACGGISPAYT LPVVLDVGTN NQQLLNDPLY MGWRNPRITD DEYYEFVDEF IQAVKQRWPD 

       250        260        270        280        290        300 
VLLQFEDFAQ KNAMPLLNRY RNEICSFNDD IQGTAAVTVG TLIAASRAAG GQLSEKKIVF 

       310        320        330        340        350        360 
LGAGSAGCGI AEMIIAQTPR EGLSEEAARQ KVFMVDRFGL LTDKMPNLLP FQTKLVQKRE 

       370        380        390        400        410        420 
NLSDWDTDSD VLSLLDVVRN VKPDILIGVS GQTGLFTEEI IREMHKHCPR PIVMPLSNPT 

       430        440        450        460        470        480 
SRVEATPQDI IAWTEGNALV ATGSPFNPVV WKDKIYPIAQ CNNAFIFPGI GLGVIASGAS 

       490        500        510        520        530        540 
RITDEMLMSA SETLAQYSPL VLNGEGLVLP ELKDIQKVSR AIAFAVGKMA QQQGVAVKTS 

       550        560 
AEALQQAIDD NFWQAEYRDY RRTSI 

« Hide

References

[1]"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery."
Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. expand/collapse author list , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q.
Nucleic Acids Res. 33:6445-6458(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Ss046.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000038 Genomic DNA. Translation: AAZ88340.1. Different initiation.
RefSeqYP_310575.2. NC_007384.1.

3D structure databases

ProteinModelPortalQ3Z1M2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300269.SSON_1645.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ88340; AAZ88340; SSON_1645.
GeneID3668674.
KEGGssn:SSON_1645.
PATRIC18737246. VBIShiSon107113_1971.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0281.
HOGENOMHOG000042487.
KOK00027.
OrthoDBEOG6QVRGM.

Enzyme and pathway databases

BioCycSSON300269:GJJF-1641-MONOMER.

Family and domain databases

Gene3D3.40.50.10380. 1 hit.
3.40.50.720. 1 hit.
HAMAPMF_01619. NAD_malic_enz.
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N_dom.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR023667. NAD_malic_enz_proteobac.
[Graphical view]
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAO1_SHISS
AccessionPrimary (citable) accession number: Q3Z1M2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families