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Protein

Gamma-glutamyl-gamma-aminobutyrate hydrolase

Gene

puuD

Organism
Shigella sonnei (strain Ss046)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma-aminobutyrate.By similarity

Catalytic activityi

4-(gamma-L-glutamylamino)butanoate + H2O = 4-aminobutanoate + L-glutamate.

Pathwayi: putrescine degradation

This protein is involved in step 4 of the subpathway that synthesizes 4-aminobutanoate from putrescine.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Gamma-glutamyl-gamma-aminobutyrate hydrolase (puuD)
This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobutanoate from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141NucleophilePROSITE-ProRule annotation
Active sitei222 – 2221PROSITE-ProRule annotation
Active sitei224 – 2241PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3693-MONOMER.
SSON300269:GJJF-1838-MONOMER.
UniPathwayiUPA00188; UER00883.

Protein family/group databases

MEROPSiC26.961.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC:3.5.1.94)
Short name:
Gamma-Glu-GABA hydrolase
Gene namesi
Name:puuD
Ordered Locus Names:SSON_1842
OrganismiShigella sonnei (strain Ss046)
Taxonomic identifieri300269 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
Proteomesi
  • UP000002529 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 254254Gamma-glutamyl-gamma-aminobutyrate hydrolasePRO_0000272689Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ3Z146.
SMRiQ3Z146. Positions 26-241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 250235Glutamine amidotransferase type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C26 family.Curated
Contains 1 glutamine amidotransferase type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

HOGENOMiHOG000035715.
KOiK09473.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR011697. Peptidase_C26.
[Graphical view]
PfamiPF07722. Peptidase_C26. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3Z146-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENIMNNPVI GVVMCRNRLK GHATQTLQEK YLNAIIHAGG LPIALPHALA
60 70 80 90 100
EPSLLEQLLP KLDGIYLPGS PSNVQPHLYG ENGDEPDADP GRDLLSMAII
110 120 130 140 150
NAALERRIPI FAICRGLQEL VVATGGSLHR KLCEQPELLE HREDPELPVE
160 170 180 190 200
QQYAPSHEVQ VEEGGLLSAL LPECSNFWVN SLHGQGAKVV SPRLRVEARS
210 220 230 240 250
PDGLVEAVSV INHPFALGVQ WHPEWNSSEY ALSRILFEGF ITACQHHIAE

KQRL
Length:254
Mass (Da):28,013
Last modified:January 23, 2007 - v2
Checksum:iED183250300CCB42
GO

Sequence cautioni

The sequence AAZ88516 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000038 Genomic DNA. Translation: AAZ88516.1. Different initiation.
RefSeqiWP_001300506.1. NC_007384.1.

Genome annotation databases

EnsemblBacteriaiAAZ88516; AAZ88516; SSON_1842.
KEGGissn:SSON_1842.
PATRICi18737758. VBIShiSon107113_2223.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000038 Genomic DNA. Translation: AAZ88516.1. Different initiation.
RefSeqiWP_001300506.1. NC_007384.1.

3D structure databases

ProteinModelPortaliQ3Z146.
SMRiQ3Z146. Positions 26-241.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC26.961.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ88516; AAZ88516; SSON_1842.
KEGGissn:SSON_1842.
PATRICi18737758. VBIShiSon107113_2223.

Phylogenomic databases

HOGENOMiHOG000035715.
KOiK09473.

Enzyme and pathway databases

UniPathwayiUPA00188; UER00883.
BioCyciRETL1328306-WGS:GSTH-3693-MONOMER.
SSON300269:GJJF-1838-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR011697. Peptidase_C26.
[Graphical view]
PfamiPF07722. Peptidase_C26. 1 hit.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPUUD_SHISS
AccessioniPrimary (citable) accession number: Q3Z146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.