Reviewed, UniProtKB/Swiss-Prot Q3YXA5 (GARL_SHISS)

Names and origin

Protein names

Recommended name:
    5-keto-4-deoxy-D-glucarate aldolase
      Short name=KDGluc aldolase
      Short name=KDGlucA
    EC=4.1.2.20
Alternative name(s):
    5-dehydro-4-deoxy-D-glucarate aldolase
    2-keto-3-deoxy-D-glucarate aldolase
    2-dehydro-3-deoxy-D-glucarate aldolase
    Alpha-keto-beta-deoxy-D-glucarate aldolase

Gene names

Name:	garL
Ordered Locus Names:	SSON_3281

Organism

Shigella sonnei (strain Ss046) [Complete proteome] [HAMAP]

Taxonomic identifier

300269 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible retro-aldol cleavage of both 5-keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde By similarity.
Catalytic activity	5-dehydro-4-deoxy-D-glucarate = pyruvate + tartronate semialdehyde. HAMAP MF_01291 2-dehydro-3-deoxy-D-glucarate = pyruvate + tartronate semialdehyde. HAMAP MF_01291
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate acid metabolism; D-galactaric acid degradation; D-glyceric acid from D-galactaric acid: step 2/3. HAMAP MF_01291
Subunit structure	Homohexamer; trimer of dimers By similarity.
Sequence similarities	Belongs to the hpcH/hpaI aldolase family. KDGluc aldolase subfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	D-galactarate catabolic process Inferred from electronic annotation. Source: HAMAP cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro glucarate catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-dehydro-3-deoxyglucarate aldolase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	5-keto-4-deoxy-D-glucarate aldolase HAMAP MF_01291		PRO_0000353162
Sites
	Active site	50	1	Proton acceptor By similarity
	Metal binding	153	1	Magnesium By similarity
	Metal binding	179	1	Magnesium By similarity
	Binding site	151	1	Substrate By similarity
	Binding site	178	1	Substrate; via amide nitrogen By similarity
	Binding site	179	1	Substrate By similarity
	Site	75	1	Transition state stabilizer By similarity
	Site	89	1	Increases basicity of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3YXA5-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: E27F293FFB0B6408	FASTA	256	27,429
	10 20 30 40 50 60 MNNDVFPNKF KAALAAKQVQ IGCWSALSNP ISTEVLGLAG FDWLVLDGEH APNDISTFIP 70 80 90 100 110 120 QLMALKGSAS APVVRVPTNE PVIIKRLLDI GFYNFLIPFV ETKEEAEQAV ASTRYPPEGI 130 140 150 160 170 180 RGVSVSHRTN MFGTVADYFA QSNKNITILV QIESQQGVDN VDAIAATEGV DGIFVGPSDL 190 200 210 220 230 240 AAALGHLGNA SHPDVQKAIQ HIFNRASAHG KPSGILAPVE ADARRYLEWG ATFVAVGSDL 250 GVFRSATQKL ADTFKK

References

[1]

"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. , Wang Y., Yuan Z., Wen Y., Yao Z., Shen Y., Qiang B., Hou Y., Yu J., Jin Q. Nucleic Acids Res. 33:6445-6458(2005) [PubMed: 16275786] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000038 Genomic DNA. Translation: AAZ89857.1.
RefSeq	YP_312092.1.
3D structure databases
SMR	Q3YXA5. Positions 4-256.
ModBase	Search...
Genome annotation databases
GeneID	3668964.
GenomeReviews	Gene locus SSON_3281 in contig CP000038_GR.
KEGG	ssn:SSON_3281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3YXA5.
OMA	Q3YXA5. QRSNRYG.
Enzyme and pathway databases
BioCyc	SSON300269:SSO_3281-MON.
Family and domain databases
HAMAP	MF_01291. [Tree]
InterPro	IPR017648. Dehyd-dGlucarate-aldolase_GarL. IPR005000. HpcH_HpaI. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF03328. HpcH_HpaI. 1 hit. [Graphical view]
ProDom	PD001009. Pyruvate_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03239. GarL. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GARL_SHISS

Accession

Primary (citable) accession number: Q3YXA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	September 27, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)