Q3YSK1 (DAPA_EHRCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Ecaj_0257

Organism

Ehrlichia canis (strain Jake) [Complete proteome] [HAMAP]

Taxonomic identifier

269484 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Ehrlichia

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050187
Sites
	Active site	165	1	Schiff-base intermediate with substrate By similarity
	Site	137	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3YSK1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 3827263918354B28	FASTA	296	32,630
	10 20 30 40 50 60 MSKVKLSGIF TALITPFKND FSIDEDTFSK LIEHQISNNI HGLVPCGTTG EYSTLSFEEY 70 80 90 100 110 120 CRVIELCVKV TNKRVPIIAG SGSNCTQETI KRTLYVQSLN VDAALVVVPY YNRPSDEGIF 130 140 150 160 170 180 QHFKAVHDAT DVPIIIYNIP QRTAIDPNDV LLARILSLPR IIGIKDATGD VSRPLNLKLL 190 200 210 220 230 240 IDKEFALFTG NDATSLGFYA QGGGTGCISA VSNVIPKIYS DMHNAFFAHN IKEAMDANAS 250 260 270 280 290 IFKLSKVLFC QSNPSPTKYA MSLIKGISPT VRLPLVELTQ ENKLKVENML KELNLI

References

[1]

"The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies." Mavromatis K., Doyle C.K., Lykidis A., Ivanova N., Francino M.P., Chain P., Shin M., Malfatti S., Larimer F., Copeland A., Detter J.C., Land M., Richardson P.M., Yu X.J., Walker D.H., McBride J.W., Kyrpides N.C. J. Bacteriol. 188:4015-4023(2006) [PubMed: 16707693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Jake.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000107 Genomic DNA. Translation: AAZ68304.1.
RefSeq	YP_302902.1. NC_007354.1.
3D structure databases
HSSP	HSSP built from PDB template 2A6N based on UniProtKB P0A6L2.
ProteinModelPortal	Q3YSK1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3YSK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3618347.
GenomeReviews	Gene locus Ecaj_0257 in contig CP000107_GR.
KEGG	ecn:Ecaj_0257.
PATRIC	20574026. VBIEhrCan118076_0277.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	HFKAVHD.
PhylomeDB	Q3YSK1.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	ECAN269484:ECAJ_0257-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_EHRCJ

Accession

Primary (citable) accession number: Q3YSK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	September 27, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program