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Q3YSH6 (SYL_EHRCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ecaj_0282
OrganismEhrlichia canis (strain Jake) [Complete proteome] [HAMAP]
Taxonomic identifier269484 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 829829Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334752

Regions

Motif34 – 4411"HIGH" region HAMAP-Rule MF_00049
Motif591 – 5955"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5941ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3YSH6 [UniParc].

Last modified September 27, 2005. Version 1.
Checksum: B2A7FC52332711ED

FASTA82994,858
        10         20         30         40         50         60 
MHYDFKKVEQ DIQKKWNFYT DVKKAQCYVL EMFPYPSGNI HMGHLRNYTI GDVIARYKRA 

        70         80         90        100        110        120 
CGINVFHPIG WDAFGLPAEN AALSYNINPQ TWTKNNIDNM RCQLKSIGLS YDWNKELATC 

       130        140        150        160        170        180 
DADYYKHEQA FFLDFLKCGL AYRKESLVNW DPIDQTVLAN EQVIDGKGWR SGAVVEKRKL 

       190        200        210        220        230        240 
SQWFLKITDF AEELLNDLKV LDKWPEKVKL MQERWIGRSE GVVVDFEILN INKTLQVFTT 

       250        260        270        280        290        300 
CPHTLFGASF IAVSFDHPIL QCVSDSDIIQ QINDFDKNNL ITDASSTVEK FGINSGLVVK 

       310        320        330        340        350        360 
HPFLCMNLPV YVVNFVLMDY ATGAVFGCPA HDQRDFEFAK KYNLQIKQVV FPEIDIDLGK 

       370        380        390        400        410        420 
EAYVGSGTMK NSDFLDGMTV DEAKEAMIAK LQLLGIGDKM TYYRIHDWGI SRQRYWGCPI 

       430        440        450        460        470        480 
PIIYCEKCGT VPVSRKDLPV TLPQDVDFTK SGNPLDNHPT WKYVKCPSCG MDAERETDTF 

       490        500        510        520        530        540 
DTFFESSWYF AAFCGTSSGI DKDTCNMLLP VDYYIGGIEH AVLHLLYSRF FCRALTKCGY 

       550        560        570        580        590        600 
FNVKEPFSSL ITQGMVCHAT YSDAQGNYLF PEEARKMMEE GQHVNVGRAE KMSKSKKNVV 

       610        620        630        640        650        660 
NLEYIIDKYG ADTARLFILS DTPPDRDIEW LDDGIEGASK YLSKLWRMIV SYDQINLNFN 

       670        680        690        700        710        720 
VEDIPEDAIK YRRCVHKIVS DITSDLEFCR LNCAVAKFRE LSNILSEMIR TSVNSDVVSE 

       730        740        750        760        770        780 
AICILIRVIE PFIPHIAEKL WENIGGKDML WNQMWPKADT ELLIERNVNI AVQVNGKFIR 

       790        800        810        820 
TLTVANNIDN DQLKSMALEM AKNKIGNSVV KNVYIIPKRA VNIVIEKLS 

« Hide

References

[1]"The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies."
Mavromatis K., Doyle C.K., Lykidis A., Ivanova N., Francino M.P., Chain P., Shin M., Malfatti S., Larimer F., Copeland A., Detter J.C., Land M., Richardson P.M., Yu X.J., Walker D.H., McBride J.W., Kyrpides N.C.
J. Bacteriol. 188:4015-4023(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Jake.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000107 Genomic DNA. Translation: AAZ68329.1.
RefSeqYP_302927.1. NC_007354.1.

3D structure databases

ProteinModelPortalQ3YSH6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269484.Ecaj_0282.

Proteomic databases

PRIDEQ3YSH6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ68329; AAZ68329; Ecaj_0282.
GeneID3617667.
KEGGecn:Ecaj_0282.
PATRIC20574082. VBIEhrCan118076_0304.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycECAN269484:GI02-301-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_EHRCJ
AccessionPrimary (citable) accession number: Q3YSH6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: September 27, 2005
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries