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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ehrlichia canis (strain Jake)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei650ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ecaj_0495
OrganismiEhrlichia canis (strain Jake)
Taxonomic identifieri269484 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
Proteomesi
  • UP000000435 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985431 – 1120Isoleucine--tRNA ligaseAdd BLAST1120

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269484.Ecaj_0495.

Structurei

3D structure databases

ProteinModelPortaliQ3YRX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi64 – 74"HIGH" regionAdd BLAST11
Motifi647 – 651"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246403.
KOiK01870.
OMAiWGTPLNI.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07961. Anticodon_Ia_Ile_ABEc. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033709. Anticodon_Ile_ABEc.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3YRX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCIVKYLSLI INTMTEHYSQ LTGEPDFPSI EENVLKFWQE NNIFKKSVDN
60 70 80 90 100
RDENKRFIFY DGPPFANGLP HYGHLLTGFI KDTVARYKTM AGFKVERRFG
110 120 130 140 150
WDCHGLPAEM LSEKELGISG KLAIEKFGIE KFNNHCRNSV MKFSKEWKQY
160 170 180 190 200
VDRQARWVDF ENDYKTMNSS FMESIIWSFH ELWNKGLIYE SIKIVPYSWA
210 220 230 240 250
CQTPLSNFET RMDNAYREKT SKTVTVAFEL LESPKFITVE NVKTYKILVW
260 270 280 290 300
TTTPWTLPCN LALAISKNIK YCGAIIKHEM LIFATGYLKI FQEHCKKNNI
310 320 330 340 350
EYQLYNQDIS SVNLEDLHYK PLFKYFADVK NAFKILTADF VVEGEGTGIV
360 370 380 390 400
HIAPGFGEDD FILCKMQDIP HIEGDTSNLL SIICPIDDGA KFTDKISDFK
410 420 430 440 450
NMHVFDTNDQ IINILKQKNL CFKIDQYLHN YPHCWRTDTP LIYRAMSSWY
460 470 480 490 500
VEVTKIKDKM IELNKTVNWI PNHICNGQFG KWLENAKDWA ISRNRFWGTP
510 520 530 540 550
LPVWKSDNPN YPRIDVYGSI RKVFNNVKAL EEDFDISSIN DLHRPYIDNL
560 570 580 590 600
VRPNPDDPTG KSMMRRVSDV FDCWFESGSM PYAQLHYPFE NKEFFENYFP
610 620 630 640 650
ADFITEYIAQ TRGWFYTLFI LSTALFNKPP FINCICHGVV LDTQGQKLSK
660 670 680 690 700
RLNNYADPME IFNQYGSDAM RFLMLSHTVL YGGDLLLDKE GVMIKDVLRN
710 720 730 740 750
VIKPIWNSYN FFTIYANIDH ITAEIITELN ELSNIMDRYI ICECINTIHS
760 770 780 790 800
IFNAMEELDQ CSNNLGYNIK LACNNITKFF EILNNWYIRR CRSRFWSSEI
810 820 830 840 850
TQDKQDAYNT LYTVIYYMIK VSAPFLPIIT EAIWQRLNFQ KEESVHLSSL
860 870 880 890 900
PNISNFILNN EDKQNIQYMK LITCICNYVL SIRSTHNIRI RQPLNKIVIY
910 920 930 940 950
SHNCPDLLNL PAEYQNILLE EVNVKSISFK SDISDIASFQ LKLNFPELGK
960 970 980 990 1000
RIPDKVKRLI FLLKNDQWKI LENDKLLLGT IEAEHYVINN NEYTLALKVH
1010 1020 1030 1040 1050
NDFACTINLD QHLLGVVLLD NELSNELIME GIARDIIRTI QHSRKDNKFN
1060 1070 1080 1090 1100
ISDKIDVIIH TKDNIVKDSI KTWSQYIIQQ TLSTSFAIHE EISDIQDINE
1110 1120
YYKTTMKDKE VSVFLKKSHT
Length:1,120
Mass (Da):131,115
Last modified:September 27, 2005 - v1
Checksum:iE982615B4B75549D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000107 Genomic DNA. Translation: AAZ68532.1.

Genome annotation databases

EnsemblBacteriaiAAZ68532; AAZ68532; Ecaj_0495.
KEGGiecn:Ecaj_0495.
PATRICi20574574. VBIEhrCan118076_0540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000107 Genomic DNA. Translation: AAZ68532.1.

3D structure databases

ProteinModelPortaliQ3YRX3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi269484.Ecaj_0495.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAZ68532; AAZ68532; Ecaj_0495.
KEGGiecn:Ecaj_0495.
PATRICi20574574. VBIEhrCan118076_0540.

Phylogenomic databases

eggNOGiENOG4105C07. Bacteria.
COG0060. LUCA.
HOGENOMiHOG000246403.
KOiK01870.
OMAiWGTPLNI.
OrthoDBiPOG091H028I.

Family and domain databases

CDDicd07961. Anticodon_Ia_Ile_ABEc. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033709. Anticodon_Ile_ABEc.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI_EHRCJ
AccessioniPrimary (citable) accession number: Q3YRX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 27, 2005
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.