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Q3YRX3

- SYI_EHRCJ

UniProt

Q3YRX3 - SYI_EHRCJ

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ehrlichia canis (strain Jake)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei650 – 6501ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciECAN269484:GI02-524-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ecaj_0495
OrganismiEhrlichia canis (strain Jake)
Taxonomic identifieri269484 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
ProteomesiUP000000435: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11201120Isoleucine--tRNA ligasePRO_0000098543Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi269484.Ecaj_0495.

Structurei

3D structure databases

ProteinModelPortaliQ3YRX3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi647 – 6515"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3YRX3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCIVKYLSLI INTMTEHYSQ LTGEPDFPSI EENVLKFWQE NNIFKKSVDN
60 70 80 90 100
RDENKRFIFY DGPPFANGLP HYGHLLTGFI KDTVARYKTM AGFKVERRFG
110 120 130 140 150
WDCHGLPAEM LSEKELGISG KLAIEKFGIE KFNNHCRNSV MKFSKEWKQY
160 170 180 190 200
VDRQARWVDF ENDYKTMNSS FMESIIWSFH ELWNKGLIYE SIKIVPYSWA
210 220 230 240 250
CQTPLSNFET RMDNAYREKT SKTVTVAFEL LESPKFITVE NVKTYKILVW
260 270 280 290 300
TTTPWTLPCN LALAISKNIK YCGAIIKHEM LIFATGYLKI FQEHCKKNNI
310 320 330 340 350
EYQLYNQDIS SVNLEDLHYK PLFKYFADVK NAFKILTADF VVEGEGTGIV
360 370 380 390 400
HIAPGFGEDD FILCKMQDIP HIEGDTSNLL SIICPIDDGA KFTDKISDFK
410 420 430 440 450
NMHVFDTNDQ IINILKQKNL CFKIDQYLHN YPHCWRTDTP LIYRAMSSWY
460 470 480 490 500
VEVTKIKDKM IELNKTVNWI PNHICNGQFG KWLENAKDWA ISRNRFWGTP
510 520 530 540 550
LPVWKSDNPN YPRIDVYGSI RKVFNNVKAL EEDFDISSIN DLHRPYIDNL
560 570 580 590 600
VRPNPDDPTG KSMMRRVSDV FDCWFESGSM PYAQLHYPFE NKEFFENYFP
610 620 630 640 650
ADFITEYIAQ TRGWFYTLFI LSTALFNKPP FINCICHGVV LDTQGQKLSK
660 670 680 690 700
RLNNYADPME IFNQYGSDAM RFLMLSHTVL YGGDLLLDKE GVMIKDVLRN
710 720 730 740 750
VIKPIWNSYN FFTIYANIDH ITAEIITELN ELSNIMDRYI ICECINTIHS
760 770 780 790 800
IFNAMEELDQ CSNNLGYNIK LACNNITKFF EILNNWYIRR CRSRFWSSEI
810 820 830 840 850
TQDKQDAYNT LYTVIYYMIK VSAPFLPIIT EAIWQRLNFQ KEESVHLSSL
860 870 880 890 900
PNISNFILNN EDKQNIQYMK LITCICNYVL SIRSTHNIRI RQPLNKIVIY
910 920 930 940 950
SHNCPDLLNL PAEYQNILLE EVNVKSISFK SDISDIASFQ LKLNFPELGK
960 970 980 990 1000
RIPDKVKRLI FLLKNDQWKI LENDKLLLGT IEAEHYVINN NEYTLALKVH
1010 1020 1030 1040 1050
NDFACTINLD QHLLGVVLLD NELSNELIME GIARDIIRTI QHSRKDNKFN
1060 1070 1080 1090 1100
ISDKIDVIIH TKDNIVKDSI KTWSQYIIQQ TLSTSFAIHE EISDIQDINE
1110 1120
YYKTTMKDKE VSVFLKKSHT
Length:1,120
Mass (Da):131,115
Last modified:September 27, 2005 - v1
Checksum:iE982615B4B75549D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000107 Genomic DNA. Translation: AAZ68532.1.
RefSeqiYP_303130.2. NC_007354.1.

Genome annotation databases

EnsemblBacteriaiAAZ68532; AAZ68532; Ecaj_0495.
GeneIDi3618203.
KEGGiecn:Ecaj_0495.
PATRICi20574574. VBIEhrCan118076_0540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000107 Genomic DNA. Translation: AAZ68532.1 .
RefSeqi YP_303130.2. NC_007354.1.

3D structure databases

ProteinModelPortali Q3YRX3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 269484.Ecaj_0495.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAZ68532 ; AAZ68532 ; Ecaj_0495 .
GeneIDi 3618203.
KEGGi ecn:Ecaj_0495.
PATRICi 20574574. VBIEhrCan118076_0540.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci ECAN269484:GI02-524-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies."
    Mavromatis K., Doyle C.K., Lykidis A., Ivanova N., Francino M.P., Chain P., Shin M., Malfatti S., Larimer F., Copeland A., Detter J.C., Land M., Richardson P.M., Yu X.J., Walker D.H., McBride J.W., Kyrpides N.C.
    J. Bacteriol. 188:4015-4023(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Jake.

Entry informationi

Entry nameiSYI_EHRCJ
AccessioniPrimary (citable) accession number: Q3YRX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: September 27, 2005
Last modified: October 29, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3