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Q3YRG6 (BIOB_EHRCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Ecaj_0657
OrganismEhrlichia canis (strain Jake) [Complete proteome] [HAMAP]
Taxonomic identifier269484 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Biotin synthase HAMAP-Rule MF_01694
PRO_0000381374

Sites

Metal binding571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding641Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1011Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1321Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1921Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2641Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3YRG6 [UniParc].

Last modified September 27, 2005. Version 1.
Checksum: 7824C0FF36C2614B

FASTA32436,442
        10         20         30         40         50         60 
MSTFTPTRND WQLDEILELF HTAFNDLILQ SHLLHRQFFS NNEVQISSLL NIKTGGCPEN 

        70         80         90        100        110        120 
CKYCSQSAHY KTDLKKESLL DIESIKKAIQ TAKKNGADRF CFAAAWRQVR DRDLEYICDI 

       130        140        150        160        170        180 
IDLIKSENLE SCASLGMVTL EQAKKLKNAG LDFYNHNIDT SRDFYPNVTT TRSYDDRLTS 

       190        200        210        220        230        240 
LNNIHEAGIN ICSGGILGLG ESVEDRAKML LTLANLKEHP LSVPINRLVP IKGTPFENNA 

       250        260        270        280        290        300 
KIDNIDFIKT IAVARILMPK SYIRLAAGRM SMSEEMQALC LFAGANSIFY GEKLLTTPNA 

       310        320 
DCNDDRNLLS KLGAKTKEPV FFNS 

« Hide

References

[1]"The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies."
Mavromatis K., Doyle C.K., Lykidis A., Ivanova N., Francino M.P., Chain P., Shin M., Malfatti S., Larimer F., Copeland A., Detter J.C., Land M., Richardson P.M., Yu X.J., Walker D.H., McBride J.W., Kyrpides N.C.
J. Bacteriol. 188:4015-4023(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Jake.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000107 Genomic DNA. Translation: AAZ68689.1.
RefSeqYP_303287.1. NC_007354.1.

3D structure databases

ProteinModelPortalQ3YRG6.
SMRQ3YRG6. Positions 8-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING269484.Ecaj_0657.

Proteomic databases

PRIDEQ3YRG6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAZ68689; AAZ68689; Ecaj_0657.
GeneID3618143.
KEGGecn:Ecaj_0657.
PATRIC20574918. VBIEhrCan118076_0707.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycECAN269484:GI02-690-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_EHRCJ
AccessionPrimary (citable) accession number: Q3YRG6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 27, 2005
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways