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Q3YRG6

- BIOB_EHRCJ

UniProt

Q3YRG6 - BIOB_EHRCJ

Protein

Biotin synthase

Gene

bioB

Organism
Ehrlichia canis (strain Jake)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (27 Sep 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi101 – 1011Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi132 – 1321Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi192 – 1921Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi264 – 2641Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciECAN269484:GI02-690-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Ecaj_0657
    OrganismiEhrlichia canis (strain Jake)
    Taxonomic identifieri269484 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeEhrlichia
    ProteomesiUP000000435: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Biotin synthasePRO_0000381374Add
    BLAST

    Proteomic databases

    PRIDEiQ3YRG6.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi269484.Ecaj_0657.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3YRG6.
    SMRiQ3YRG6. Positions 8-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3YRG6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTFTPTRND WQLDEILELF HTAFNDLILQ SHLLHRQFFS NNEVQISSLL    50
    NIKTGGCPEN CKYCSQSAHY KTDLKKESLL DIESIKKAIQ TAKKNGADRF 100
    CFAAAWRQVR DRDLEYICDI IDLIKSENLE SCASLGMVTL EQAKKLKNAG 150
    LDFYNHNIDT SRDFYPNVTT TRSYDDRLTS LNNIHEAGIN ICSGGILGLG 200
    ESVEDRAKML LTLANLKEHP LSVPINRLVP IKGTPFENNA KIDNIDFIKT 250
    IAVARILMPK SYIRLAAGRM SMSEEMQALC LFAGANSIFY GEKLLTTPNA 300
    DCNDDRNLLS KLGAKTKEPV FFNS 324
    Length:324
    Mass (Da):36,442
    Last modified:September 27, 2005 - v1
    Checksum:i7824C0FF36C2614B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000107 Genomic DNA. Translation: AAZ68689.1.
    RefSeqiWP_011304766.1. NC_007354.1.
    YP_303287.1. NC_007354.1.

    Genome annotation databases

    EnsemblBacteriaiAAZ68689; AAZ68689; Ecaj_0657.
    GeneIDi3618143.
    KEGGiecn:Ecaj_0657.
    PATRICi20574918. VBIEhrCan118076_0707.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000107 Genomic DNA. Translation: AAZ68689.1 .
    RefSeqi WP_011304766.1. NC_007354.1.
    YP_303287.1. NC_007354.1.

    3D structure databases

    ProteinModelPortali Q3YRG6.
    SMRi Q3YRG6. Positions 8-318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 269484.Ecaj_0657.

    Proteomic databases

    PRIDEi Q3YRG6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAZ68689 ; AAZ68689 ; Ecaj_0657 .
    GeneIDi 3618143.
    KEGGi ecn:Ecaj_0657.
    PATRICi 20574918. VBIEhrCan118076_0707.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci ECAN269484:GI02-690-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the obligately intracellular bacterium Ehrlichia canis reveals themes of complex membrane structure and immune evasion strategies."
      Mavromatis K., Doyle C.K., Lykidis A., Ivanova N., Francino M.P., Chain P., Shin M., Malfatti S., Larimer F., Copeland A., Detter J.C., Land M., Richardson P.M., Yu X.J., Walker D.H., McBride J.W., Kyrpides N.C.
      J. Bacteriol. 188:4015-4023(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Jake.

    Entry informationi

    Entry nameiBIOB_EHRCJ
    AccessioniPrimary (citable) accession number: Q3YRG6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: September 27, 2005
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3