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Q3Y5Z3

- ADIPO_BOVIN

UniProt

Q3Y5Z3 - ADIPO_BOVIN

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Protein
Adiponectin
Gene
ADIPOQ, ACRP30, APM1
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei57 – 571Not hydroxylated
Sitei66 – 661Not hydroxylated
Sitei71 – 711Not hydroxylated
Sitei90 – 901Not hydroxylated
Sitei99 – 991Not hydroxylated
Sitei225 – 2251Not glycosylated

GO - Molecular functioni

  1. hormone activity Source: UniProtKB
  2. identical protein binding Source: IntAct
  3. receptor binding Source: UniProtKB
  4. sialic acid binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. adiponectin-activated signaling pathway Source: InterPro
  2. brown fat cell differentiation Source: UniProtKB
  3. cellular response to drug Source: UniProtKB
  4. cellular response to insulin stimulus Source: UniProtKB
  5. detection of oxidative stress Source: UniProtKB
  6. fatty acid beta-oxidation Source: UniProtKB
  7. fatty acid oxidation Source: UniProtKB
  8. glucose homeostasis Source: UniProtKB
  9. glucose metabolic process Source: UniProtKB
  10. low-density lipoprotein particle clearance Source: UniProtKB
  11. negative regulation of DNA biosynthetic process Source: UniProtKB
  12. negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  13. negative regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  14. negative regulation of MAP kinase activity Source: UniProtKB
  15. negative regulation of blood pressure Source: UniProtKB
  16. negative regulation of cell migration Source: UniProtKB
  17. negative regulation of fat cell differentiation Source: UniProtKB
  18. negative regulation of gluconeogenesis Source: UniProtKB
  19. negative regulation of granulocyte differentiation Source: UniProtKB
  20. negative regulation of heterotypic cell-cell adhesion Source: UniProtKB
  21. negative regulation of inflammatory response Source: UniProtKB
  22. negative regulation of intracellular protein transport Source: UniProtKB
  23. negative regulation of low-density lipoprotein particle receptor biosynthetic process Source: UniProtKB
  24. negative regulation of macrophage derived foam cell differentiation Source: UniProtKB
  25. negative regulation of macrophage differentiation Source: UniProtKB
  26. negative regulation of metanephric mesenchymal cell migration Source: UniProtKB
  27. negative regulation of phagocytosis Source: UniProtKB
  28. negative regulation of platelet-derived growth factor receptor signaling pathway Source: UniProtKB
  29. negative regulation of platelet-derived growth factor receptor-alpha signaling pathway Source: UniProtKB
  30. negative regulation of protein autophosphorylation Source: UniProtKB
  31. negative regulation of receptor binding Source: UniProtKB
  32. negative regulation of smooth muscle cell migration Source: UniProtKB
  33. negative regulation of smooth muscle cell proliferation Source: UniProtKB
  34. negative regulation of synaptic transmission Source: UniProtKB
  35. negative regulation of transcription, DNA-templated Source: UniProtKB
  36. negative regulation of tumor necrosis factor production Source: UniProtKB
  37. negative regulation of tumor necrosis factor-mediated signaling pathway Source: UniProtKB
  38. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  39. positive regulation of cAMP-dependent protein kinase activity Source: UniProtKB
  40. positive regulation of cellular protein metabolic process Source: BHF-UCL
  41. positive regulation of cholesterol efflux Source: BHF-UCL
  42. positive regulation of fatty acid metabolic process Source: UniProtKB
  43. positive regulation of glucose import Source: UniProtKB
  44. positive regulation of glycogen (starch) synthase activity Source: UniProtKB
  45. positive regulation of interleukin-8 production Source: UniProtKB
  46. positive regulation of metanephric glomerular visceral epithelial cell development Source: UniProtKB
  47. positive regulation of monocyte chemotactic protein-1 production Source: UniProtKB
  48. positive regulation of myeloid cell apoptotic process Source: UniProtKB
  49. positive regulation of protein kinase A signaling Source: UniProtKB
  50. positive regulation of protein phosphorylation Source: UniProtKB
  51. positive regulation of renal albumin absorption Source: UniProtKB
  52. positive regulation of signal transduction Source: UniProtKB
  53. protein heterotrimerization Source: Ensembl
  54. protein homooligomerization Source: UniProtKB
  55. protein localization to plasma membrane Source: UniProtKB
  56. regulation of glucose metabolic process Source: UniProtKB
  57. response to glucose Source: UniProtKB
  58. response to tumor necrosis factor Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiREACT_220799. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Adiponectin
Alternative name(s):
30 kDa adipocyte complement-related protein
Adipocyte complement-related 30 kDa protein
Short name:
ACRP30
Adipocyte, C1q and collagen domain-containing protein
Adipose most abundant gene transcript 1 protein
Short name:
apM-1
Gene namesi
Name:ADIPOQ
Synonyms:ACRP30, APM1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 1

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. cell periphery Source: UniProtKB
  2. cell surface Source: BHF-UCL
  3. collagen trimer Source: UniProtKB-KW
  4. endoplasmic reticulum Source: UniProtKB
  5. extracellular space Source: UniProtKB
  6. perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17172 Publications
Add
BLAST
Chaini18 – 240223Adiponectin
PRO_0000236269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 2815-hydroxylysine1 Publication
Glycosylationi28 – 281O-linked (Gal...)1 Publication
Disulfide bondi31 – 31Interchain; in form MMW and form HMW By similarity
Modified residuei39 – 3914-hydroxyproline1 Publication
Modified residuei42 – 4214-hydroxyproline1 Publication
Modified residuei48 – 4814-hydroxyproline1 Publication
Modified residuei60 – 6015-hydroxylysine1 Publication
Glycosylationi60 – 601O-linked (Gal...)1 Publication
Modified residuei63 – 6315-hydroxylysine1 Publication
Glycosylationi63 – 631O-linked (Gal...)1 Publication
Modified residuei72 – 7215-hydroxylysine1 Publication
Glycosylationi72 – 721O-linked (Gal...)
Modified residuei86 – 8614-hydroxyproline1 Publication
Modified residuei96 – 9615-hydroxylysine1 Publication
Glycosylationi96 – 961O-linked (Gal...)1 Publication

Post-translational modificationi

HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes By similarity.2 Publications
O-glycosylated. O-linked glycans on hydroxylysine residues consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups By similarity. O-linked glycosylations elsewhere disialylated with the structure Neu5Acalpha2->8Neu5Acalpha2->3Gal. Sialylated by alpha 2,8-sialyltransferase III. Desialylated forms are rapidly cleared from the circulation. Not N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiQ3Y5Z3.
PRIDEiQ3Y5Z3.

Interactioni

Subunit structurei

Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely additionally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2. Interacts with CTRP9 via the C1q domain (heterotrimeric complex) By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-7264459,EBI-7264459

Protein-protein interaction databases

MINTiMINT-4563461.
STRINGi9913.ENSBTAP00000026395.

Structurei

3D structure databases

ProteinModelPortaliQ3Y5Z3.
SMRiQ3Y5Z3. Positions 103-237.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 10260Collagen-like
Add
BLAST
Domaini103 – 240138C1q
Add
BLAST

Sequence similaritiesi

Contains 1 C1q domain.

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiNOG136972.
GeneTreeiENSGT00750000117356.
HOGENOMiHOG000085653.
HOVERGENiHBG108220.
InParanoidiQ3Y5Z3.
KOiK07296.
OMAiHITVYLK.
OrthoDBiEOG70ZZPW.
TreeFamiTF329591.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR028572. Adiponectin.
IPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PANTHERiPTHR24022:SF81. PTHR24022:SF81. 1 hit.
PfamiPF00386. C1q. 1 hit.
PF01391. Collagen. 1 hit.
[Graphical view]
PRINTSiPR00007. COMPLEMNTC1Q.
SMARTiSM00110. C1Q. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS50871. C1Q. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3Y5Z3-1 [UniParc]FASTAAdd to Basket

« Hide

MLLQGALLLL LALPSHGEDN MEDPPLPKGA CAGWMAGIPG HPGHNGTPGR    50
DGRDGTPGEK GEKGDPGLVG PKGDTGETGI TGIEGPRGFP GTPGRKGEPG 100
ESAYVYRSAF SVGLERQVTV PNVPIRFTKI FYNQQNHYDG TTGKFLCNIP 150
GLYYFSYHIT VYLKDVKVSL YKNDKALLFT HDQFQDKNVD QASGSVLLYL 200
EKGDQVWLQV YEGENHNGVY ADNVNDSTFT GFLLYHNIVE 240
Length:240
Mass (Da):26,133
Last modified:September 27, 2005 - v1
Checksum:i409122E49F3AF253
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661P → A in AAK58902. 1 Publication
Sequence conflicti69 – 691V → L in AAK58902. 1 Publication
Sequence conflicti74 – 741D → E in AAK58902. 1 Publication
Sequence conflicti77 – 837ETGITGI → DVGMTGA in AAK58902. 1 Publication
Sequence conflicti102 – 1021S → A in AAK58902. 1 Publication
Sequence conflicti116 – 1172RQ → TR in AAK58902. 1 Publication
Sequence conflicti141 – 1411T → S in AAK58902. 1 Publication
Sequence conflicti146 – 1461L → Y in AAK58902. 1 Publication
Sequence conflicti163 – 1631L → M in AAK58902. 1 Publication
Sequence conflicti171 – 1733YKN → FKK in AAK58902. 1 Publication
Sequence conflicti177 – 1771L → V in AAK58902. 1 Publication
Sequence conflicti181 – 1811H → Y in AAK58902. 1 Publication
Sequence conflicti184 – 1863FQD → YQE in AAK58902. 1 Publication
Sequence conflicti199 – 1991Y → H in AAK58902. 1 Publication
Sequence conflicti202 – 2021K → V in AAK58902. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF269230 mRNA. Translation: AAK58902.1.
DQ156120 Genomic DNA. Translation: AAZ81421.1.
BC140488 mRNA. Translation: AAI40489.1.
RefSeqiNP_777167.1. NM_174742.2.
UniGeneiBt.109637.

Genome annotation databases

EnsembliENSBTAT00000026395; ENSBTAP00000026395; ENSBTAG00000019813.
GeneIDi282865.
KEGGibta:282865.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF269230 mRNA. Translation: AAK58902.1 .
DQ156120 Genomic DNA. Translation: AAZ81421.1 .
BC140488 mRNA. Translation: AAI40489.1 .
RefSeqi NP_777167.1. NM_174742.2.
UniGenei Bt.109637.

3D structure databases

ProteinModelPortali Q3Y5Z3.
SMRi Q3Y5Z3. Positions 103-237.
ModBasei Search...

Protein-protein interaction databases

MINTi MINT-4563461.
STRINGi 9913.ENSBTAP00000026395.

Proteomic databases

PaxDbi Q3Y5Z3.
PRIDEi Q3Y5Z3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000026395 ; ENSBTAP00000026395 ; ENSBTAG00000019813 .
GeneIDi 282865.
KEGGi bta:282865.

Organism-specific databases

CTDi 9370.

Phylogenomic databases

eggNOGi NOG136972.
GeneTreei ENSGT00750000117356.
HOGENOMi HOG000085653.
HOVERGENi HBG108220.
InParanoidi Q3Y5Z3.
KOi K07296.
OMAi HITVYLK.
OrthoDBi EOG70ZZPW.
TreeFami TF329591.

Enzyme and pathway databases

Reactomei REACT_220799. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

NextBioi 20806393.

Family and domain databases

Gene3Di 2.60.120.40. 1 hit.
InterProi IPR028572. Adiponectin.
IPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view ]
PANTHERi PTHR24022:SF81. PTHR24022:SF81. 1 hit.
Pfami PF00386. C1q. 1 hit.
PF01391. Collagen. 1 hit.
[Graphical view ]
PRINTSi PR00007. COMPLEMNTC1Q.
SMARTi SM00110. C1Q. 1 hit.
[Graphical view ]
SUPFAMi SSF49842. SSF49842. 1 hit.
PROSITEi PS50871. C1Q. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and adipocyte differentiation-dependent expression of the unique disialic acid residue in an adipose tissue-specific glycoprotein, adipo Q."
    Sato C., Yasukawa Z., Honda N., Matsuda T., Kitajima K.
    J. Biol. Chem. 276:28849-28856(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-26; 194-197; 203-208 AND 221-228, GLYCOSYLATION, STRUCTURE OF CARBOHYDRATES.
    Tissue: Adipose tissue.
  2. Morsci N.S., Schnabel R.D., Taylor J.F.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.
  4. "Proteomic and functional characterization of endogenous adiponectin purified from fetal bovine serum."
    Wang Y., Lu G., Wong W.P.S., Vliegenthart J.F.G., Gerwig G.J., Lam K.S.L., Cooper G.J.S., Xu A.
    Proteomics 4:3933-3942(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-26, PARTIAL PROTEIN SEQUENCE, HYDROXYLATION AT LYS-28; PRO-39; PRO-42; PRO-48; LYS-60; LYS-63; LYS-72; PRO-86 AND LYS-96, GLYCOSYLATION AT LYS-28; LYS-60; LYS-63 AND LYS-96, ABSENCE OF HYDROXYLATION AT PRO-57; PRO-66; PRO-71; PRO-90 AND PRO-99, ABSENCE OF GLYCOSYLATION AT ASN-225, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiADIPO_BOVIN
AccessioniPrimary (citable) accession number: Q3Y5Z3
Secondary accession number(s): A5D7A8, Q95MQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: September 27, 2005
Last modified: September 3, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW-complex secretion By similarity.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi