Q3XZW8 (Q3XZW8_ENTFC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate racemase HAMAP MF_00258
EC=5.1.1.3 HAMAP MF_00258

Gene names

Name:	murI HAMAP MF_00258
ORF Names:	EfaeDRAFT_1012 EMBL EAN09750.1

Organism

Enterococcus faecium DO EMBL EAN09750.1

Taxonomic identifier

333849 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides the (R)-glutamate required for cell wall biosynthesis By similarity. HAMAP MF_00258 SAAS SAAS015942
Catalytic activity	L-glutamate = D-glutamate. HAMAP MF_00258 SAAS SAAS015942
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00258 SAAS SAAS015942
Sequence similarities	Belongs to the aspartate/glutamate racemases family. HAMAP MF_00258

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation HAMAP MF_00258 SAAS SAAS015942 Peptidoglycan synthesis HAMAP MF_00258 SAAS SAAS015942
Molecular function	Isomerase HAMAP MF_00258 SAAS SAAS015942 EMBL EAN09750.1
Technical term	3D-structure PDB 2JFW PDB 2JFV PDB 2JFU
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glutamate racemase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q3XZW8 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: E5162706EEFEA2CA	FASTA	277	30,199
	10 20 30 40 50 60 MKKRMIRLTD NRPIGFIDSG VGGLTVVKEA LKQLPNENIL FVGDTARCPY GPRPAEQVIQ 70 80 90 100 110 120 YTWEMTDYLV EQGIKMLVIA CNTATAVALE EIKAALSIPV IGVILPGTRA AVKKTQNKQV 130 140 150 160 170 180 GIIGTIGTVK SQAYEKALKE KVPELTVTSL ACPKFVSVVE SNEYHSSVAK KIVAETLAPL 190 200 210 220 230 240 TTKKIDTLIL GCTHYPLLRP IIQNVMGENV QLIDSGAETV GEVSMLLDYF NLSNSPQNGR 250 260 270 TLCQFYTTGS AKLFEEIAED WLGIGHLNVE HIELGGK

References

[1]	"Sequencing of the draft genome and assembly of Enterococcus faecium DO." US DOE Joint Genome Institute (JGI-PGF) Copeland A., Lucas S., Lapidus A., Barry K., Detter C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DO EMBL EAN09750.1.
[2]	"Annotation of the draft genome assembly of Enterococcus faecium DO." US DOE Joint Genome Institute (JGI-ORNL) Larimer F., Land M. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DO EMBL EAN09750.1.
[3]	US DOE Joint Genome Institute (JGI-PGF) Copeland A., Lucas S., Lapidus A., Barry K., Detter C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P. Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: DO EMBL EAN09750.1.
[4]	"Exploitation of structural and regulatory diversity in glutamate racemases." Lundqvist T., Fisher S.L., Kern G., Folmer R.H., Xue Y., Newton D.T., Keating T.A., Alm R.A., de Jonge B.L. Nature 447:817-822(2007) [PubMed: 17568739] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) OF 4-277.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AAAK03000027 Genomic DNA. Translation: EAN09750.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JFU	X-ray	1.80	A	4-277	[»]
2JFV	X-ray	1.80	A	4-277	[»]
2JFW	X-ray	2.00	A	4-277	[»]

ProteinModelPortal

Q3XZW8.

SMR

Q3XZW8. Positions 5-275.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_00258. Glu_racemase.
[Tree]

InterPro

IPR015942. Asp/Glu/hydantoin_racemase.
IPR001920. Asp/Glu_race.
IPR018187. Asp/Glu_racemase_AS.
IPR004391. Glu_race.
[Graphical view]

Gene3D

G3DSA:3.40.50.1860. Asp/Glu_race. 1 hit.

PANTHER

PTHR21198. PTHR21198. 1 hit.

Pfam

PF01177. Asp_Glu_race. 1 hit.
[Graphical view]

SUPFAM

SSF53681. Asp/Glu_race. 2 hits.

TIGRFAMs

TIGR00067. Glut_race. 1 hit.

PROSITE

PS00923. ASP_GLU_RACEMASE_1. 1 hit.
PS00924. ASP_GLU_RACEMASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q3XZW8_ENTFC

Accession

Primary (citable) accession number: Q3XZW8

Entry history

Integrated into UniProtKB/TrEMBL:	October 11, 2005
Last sequence update:	October 11, 2005
Last modified:	October 19, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)