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Q3XXK8 (Q3XXK8_ENTFC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddlA EMBL AFK57802.1
Synonyms:ddl HAMAP-Rule MF_00047
ORF Names:EfaeDRAFT_0577 EMBL EAN08955.1, HMPREF0351_10178 EMBL AFK57802.1
OrganismEnterococcus faecium DO [Complete proteome] EMBL EAN08955.1
Taxonomic identifier333849 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus

Protein attributes

Sequence length358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain136 – 341206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2951Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3101Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
Q3XXK8 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 0801F31DB1FA9FEC

FASTA35840,247
        10         20         30         40         50         60 
MKITLLYGGR SAEHEVSILS AFSVLNAIYY NYYQVQLVFI TKEGQWVKGP LLTEKPASKD 

        70         80         90        100        110        120 
VLHLSWDPSG QTEEGFTGKV INPGEIKEEG AIVFPVLHGP NGEDGTIQGF LETLNMPYVG 

       130        140        150        160        170        180 
AGVLTSACAM DKIMTKYILQ AAGVPQVPYV PVLKNQWKEN PKKVFDQCEG SLLYPMFVKP 

       190        200        210        220        230        240 
ANMGSSVGIT KAENREELQN ALATAYQYDS RAIVEQGIEA REIEVAVLGN EDVRTTLPGE 

       250        260        270        280        290        300 
VVKDVAFYDY EAKYINNKIE MQIPAEVPEE VYQKAQEYAK LAYTMLGGSG LSRCDFFLTN 

       310        320        330        340        350 
KNELFLNELN SMPGFTEFSM YPLLWENMGL KYGDLIEELI QLGMNRYHQR QSFFEKNE 

« Hide

References

[1]DOE Joint Genome Institute
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DO EMBL EAN08955.1.
[2]"Annotation of the draft genome assembly of Enterococcus faecium DO."
US DOE Joint Genome Institute (JGI-ORNL)
Larimer F., Land M.
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DO EMBL EAN08955.1.
[3]"Sequencing of the draft genome and assembly of Enterococcus faecium DO."
US DOE Joint Genome Institute (JGI-PGF)
Copeland A., Lucas S., Lapidus A., Barry K., Detter C., Glavina T., Hammon N., Israni S., Pitluck S., Richardson P.
Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DO EMBL EAN08955.1.
[4]"Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes."
Qin X., Galloway-Pena J.R., Sillanpaa J., Hyeob Roh J., Nallapareddy S.R., Chowdhury S., Bourgogne A., Choudhury T., Munzy D.M., Buhay C.J., Ding Y., Dugan-Rocha S., Liu W., Kovar C., Sodergren E., Highlander S., Petrosino J.F., Worley K.C. expand/collapse author list , Gibbs R.A., Weinstock G.M., Murray B.E.
BMC Microbiol. 12:135-135(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DO EMBL AFK57802.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003583 Genomic DNA. Translation: AFK57802.1.
AAAK03000077 Genomic DNA. Translation: EAN08955.1.
RefSeqYP_006374784.1. NC_017960.1.

3D structure databases

ProteinModelPortalQ3XXK8.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFK57802; AFK57802; HMPREF0351_10178.
EAN08955; EAN08955; EfaeDRAFT_0577.
GeneID12998837.
KEGGefu:HMPREF0351_10178.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycEFAE333849:GLCV-178-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ3XXK8_ENTFC
AccessionPrimary (citable) accession number: Q3XXK8
Entry history
Integrated into UniProtKB/TrEMBL: October 11, 2005
Last sequence update: October 11, 2005
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)