Q3V818 (PDXA_SYMTH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenase
EC=1.1.1.262

Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase

Gene names

Name:	pdxA
Ordered Locus Names:	STH2300

Organism

Symbiobacterium thermophilum (strain T / IAM 14863) [Complete proteome] [HAMAP]

Taxonomic identifier

292459 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiales incertae sedis › Clostridiales Family XVIII. Incertae Sedis › Symbiobacterium › Symbiobacterium thermophilum

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536
Catalytic activity	4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The active site is located at the dimer interface By similarity. HAMAP-Rule MF_00536
Sequence similarities	Belongs to the PdxA family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-hydroxythreonine-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	4-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536		PRO_1000211916
Sites
	Metal binding	170	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	214	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	269	1	Divalent metal cation; shared with dimeric partner By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	277	1	Substrate By similarity
	Binding site	295	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3V818 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: C6B4D45E97B0DEDE	FASTA	335	36,102
	10 20 30 40 50 60 MDTRPLIGIT MGDPASIGPE IAAKALANPE IYALCRPLLI GDSRVMARAF ETTGVKLNLN 70 80 90 100 110 120 PVATPAEGKY AHGTVDLIDL PVVDMGTLQW GQVQAQAGAA AFAYIKRSIE LALEGAVDAV 130 140 150 160 170 180 TTGPINKEAL KAARIDFIGH TEIFGELTGS HDPLTMFETK GLRIFFLTRH VSLAQACRMI 190 200 210 220 230 240 TRDRVLDYIR RCTAALEQLG LVRPKLAVAG LNPHCGEHGL FGDEEVREIE PAVQQAQAEG 250 260 270 280 290 300 YNVSGPHPAD SVFWHAAQGR FDAVLSLYHD QGHIAAKMYD FERTVSITAG LPFLRSSVDH 310 320 330 GTAFDIAGTG RASAVSLEEA IRVGAKYAAA FRRTR

References

[1]

"Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism." Ueda K., Yamashita A., Ishikawa J., Shimada M., Watsuji T., Morimura K., Ikeda H., Hattori M., Beppu T. Nucleic Acids Res. 32:4937-4944(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: T / IAM 14863.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006840 Genomic DNA. Translation: BAD41285.1.
RefSeq	YP_076129.1. NC_006177.1.
3D structure databases
ProteinModelPortal	Q3V818.
ModBase	Search...
Protein-protein interaction databases
STRING	292459.STH2300.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAD41285; BAD41285; STH2300.
GeneID	2979467.
KEGG	sth:STH2300.
PATRIC	23782778. VBISymThe116959_2303.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1995.
HOGENOM	HOG000221593.
KO	K00097.
OMA	EISVATM.
ProtClustDB	PRK03743.
Enzyme and pathway databases
UniPathway	UPA00244; UER00312.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
HAMAP	MF_00536. PdxA.
InterPro	IPR024084. IsoPropMal-DH-like_dom. IPR005255. PyrdxlP_synth_PdxA. [Graphical view]
Pfam	PF04166. PdxA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00557. pdxA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXA_SYMTH

Accession

Primary (citable) accession number: Q3V818

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	October 11, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program