Q3V6T2 (GRDN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Girdin

Alternative name(s):
Akt phosphorylation enhancer
Short name=APE
Coiled-coil domain-containing protein 88A
G alpha-interacting vesicle-associated protein
Short name=GIV
Girders of actin filament
Hook-related protein 1
Short name=HkRP1

Gene names

Name:	CCDC88A
Synonyms:	APE, GRDN, KIAA1212

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1871 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation By similarity. Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Ref.1 Ref.2
Subunit structure	Interacts (via C-terminus) with DISC1; the interaction is direct. Interacts with AKT proteins; the interaction is inhibited in presence of DISC1 By similarity. Homodimer. The non-phosphorylated form interacts with phosphatidylinositol 4-phosphate [PI4P] and weakly with phosphatidylinositol 3-phosphate [PI3P]. Interacts with microtubules. Interacts with actin through its C-terminal domain. Interacts with the C-terminus of AKT1/PKB. Ref.1
Subcellular location	Membrane. Cell membrane. Cytoplasm › cytosol. Cytoplasmic vesicle. Cell projection › lamellipodium. Note: Localizes to the cell membrane through interaction with phosphoinositides. Ref.1 Ref.2 Ref.7
Tissue specificity	Expressed ubiquitously. Ref.1
Post-translational modification	Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration. Ref.1
Sequence similarities	Belongs to the CCDC88 family.
Sequence caution	The sequence AAY14932.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAD97945.1 differs from that shown. Reason: Intron retention at the C-terminus. The sequence CAI46020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	DNA replication Neurogenesis
Cellular component	Cell membrane Cell projection Cytoplasm Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW TOR signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB activation of protein kinase B activity Inferred from sequence or structural similarity. Source: UniProtKB cell migration Inferred from mutant phenotype Ref.1. Source: UniProtKB cellular membrane organization Inferred from direct assay Ref.2. Source: UniProtKB lamellipodium assembly Inferred from mutant phenotype Ref.1. Source: UniProtKB microtubule cytoskeleton organization Inferred from electronic annotation. Source: InterPro regulation of DNA replication Inferred from sequence or structural similarity. Source: UniProtKB regulation of actin cytoskeleton organization Inferred from mutant phenotype Ref.1. Source: UniProtKB regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB regulation of neuron projection development Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay Ref.2. Source: UniProtKB cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic vesicle Inferred from direct assay Ref.2. Source: UniProtKB cytosol Inferred from direct assay Ref.7. Source: UniProtKB endoplasmic reticulum Inferred from direct assay Ref.2. Source: UniProtKB lamellipodium Inferred from direct assay Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	actin binding Inferred from direct assay Ref.1. Source: UniProtKB microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB phosphatidylinositol binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 Ref.6 (identifier: Q3V6T2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.2 Ref.3 Ref.5 Ref.6 (identifier: Q3V6T2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1463-1491: MVALKRLPFLRNRPKDKDKMKACYRRSMS → T

Isoform 3 (identifier: Q3V6T2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     952-952: Missing.

Isoform 4 (identifier: Q3V6T2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     952-952: Missing.
     1463-1491: MVALKRLPFLRNRPKDKDKMKACYRRSMS → T

Isoform 5 (identifier: Q3V6T2-5)

The sequence of this isoform differs from the canonical sequence as follows:
     952-952: Missing.
     1733-1806: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1871	1871	Girdin		PRO_0000287429
Regions
	Region	1390 – 1408	19	Phosphoinositide-binding Ref.1
	Coiled coil	196 – 425	230	Potential
	Coiled coil	458 – 1385	928	Potential
Amino acid modifications
	Modified residue	1387	1	Phosphoserine Ref.9 PubMed 16964243
	Modified residue	1417	1	Phosphoserine; by PKB/AKT1 Ref.1
	Modified residue	1559	1	Phosphothreonine By similarity UniProtKB Q5SNZ0
	Modified residue	1560	1	Phosphothreonine By similarity UniProtKB Q5SNZ0
	Modified residue	1561	1	Phosphoserine By similarity UniProtKB Q5SNZ0
	Modified residue	1673	1	Phosphothreonine Ref.9
	Modified residue	1675	1	Phosphoserine By similarity UniProtKB Q5SNZ0
	Modified residue	1702	1	Phosphoserine By similarity UniProtKB Q5SNZ0
	Modified residue	1837	1	Phosphoserine Ref.9
Natural variations
	Alternative sequence	952	1	Missing in isoform 3, isoform 4 and isoform 5.		VSP_040129
	Alternative sequence	1463 – 1491	29	MVALK…RRSMS → T in isoform 2 and isoform 4. Ref.2 Ref.3 Ref.5 Ref.6		VSP_052409
	Alternative sequence	1733 – 1806	74	Missing in isoform 5.		VSP_044943
Experimental info
	Mutagenesis	1417	1	S → A: Disrupts actin organization, cell migration and lamellipodia formation. Ref.1
	Sequence conflict	1066	1	K → E in CAI46020. Ref.6
	Sequence conflict	1081	1	A → T in CAD97945. Ref.6
	Sequence conflict	1661	1	S → P in CAD97945. Ref.6
	Sequence conflict	1710	1	V → I in CAI46020. Ref.6
	Sequence conflict	1799	1	Y → H in CAD98038. Ref.6
	Sequence conflict	1836	1	D → G in CAI46020. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 15, 2007. Version 2. Checksum: 9DD2E06F2A235AA5	FASTA	1,871	216,042
	10 20 30 40 50 60 MENEIFTPLL EQFMTSPLVT WVKTFGPLAA GNGTNLDEYV ALVDGVFLNQ VMLQINPKLE 70 80 90 100 110 120 SQRVNKKVNN DASLRMHNLS ILVRQIKFYY QETLQQLIMM SLPNVLIIGK NPFSEQGTEE 130 140 150 160 170 180 VKKLLLLLLG CAVQCQKKEE FIERIQGLDF DTKAAVAAHI QEVTHNQENV FDLQWMEVTD 190 200 210 220 230 240 MSQEDIEPLL KNMALHLKRL IDERDEHSET IIELSEERDG LHFLPHASSS AQSPCGSPGM 250 260 270 280 290 300 KRTESRQHLS VELADAKAKI RRLRQELEEK TEQLLDCKQE LEQMEIELKR LQQENMNLLS 310 320 330 340 350 360 DARSARMYRD ELDALREKAV RVDKLESEVS RYKERLHDIE FYKARVEELK EDNQVLLETK 370 380 390 400 410 420 TMLEDQLEGT RARSDKLHEL EKENLQLKAK LHDMEMERDM DRKKIEELME ENMTLEMAQK 430 440 450 460 470 480 QSMDESLHLG WELEQISRTS ELSEAPQKSL GHEVNELTSS RLLKLEMENQ SLTKTVEELR 490 500 510 520 530 540 TTVDSVEGNA SKILKMEKEN QRLSKKVEIL ENEIVQEKQS LQNCQNLSKD LMKEKAQLEK 550 560 570 580 590 600 TIETLRENSE RQIKILEQEN EHLNQTVSSL RQRSQISAEA RVKDIEKENK ILHESIKETS 610 620 630 640 650 660 SKLSKIEFEK RQIKKELEHY KEKGERAEEL ENELHHLEKE NELLQKKITN LKITCEKIEA 670 680 690 700 710 720 LEQENSELER ENRKLKKTLD SFKNLTFQLE SLEKENSQLD EENLELRRNV ESLKCASMKM 730 740 750 760 770 780 AQLQLENKEL ESEKEQLKKG LELLKASFKK TERLEVSYQG LDIENQRLQK TLENSNKKIQ 790 800 810 820 830 840 QLESELQDLE MENQTLQKNL EELKISSKRL EQLEKENKSL EQETSQLEKD KKQLEKENKR 850 860 870 880 890 900 LRQQAEIKDT TLEENNVKIG NLEKENKTLS KEIGIYKESC VRLKELEKEN KELVKRATID 910 920 930 940 950 960 IKTLVTLRED LVSEKLKTQQ MNNDLEKLTH ELEKIGLNKE RLLHDEQSTD DSRYKLLESK 970 980 990 1000 1010 1020 LESTLKKSLE IKEEKIAALE ARLEESTNYN QQLRQELKTV KKNYEALKQR QDEERMVQSS 1030 1040 1050 1060 1070 1080 PPISGEDNKW ERESQETTRE LLKVKDRLIE VERNNATLQA EKQALKTQLK QLETQNNNLQ 1090 1100 1110 1120 1130 1140 AQILALQRQT VSLQEQNTTL QTQNAKLQVE NSTLNSQSTS LMNQNAQLLI QQSSLENENE 1150 1160 1170 1180 1190 1200 SVIKEREDLK SLYDSLIKDH EKLELLHERQ ASEYESLISK HGTLKSAHKN LEVEHRDLED 1210 1220 1230 1240 1250 1260 RYNQLLKQKG QLEDLEKMLK VEQEKMLLEN KNHETVAAEY KKLCGENDRL NHTYSQLLKE 1270 1280 1290 1300 1310 1320 TEVLQTDHKN LKSLLNNSKL EQTRLEAEFS KLKEQYQQLD ITSTKLNNQC ELLSQLKGNL 1330 1340 1350 1360 1370 1380 EEENRHLLDQ IQTLMLQNRT LLEQNMESKD LFHVEQRQYI DKLNELRRQK EKLEEKIMDQ 1390 1400 1410 1420 1430 1440 YKFYDPSPPR RRGNWITLKM RKLIKSKKDI NRERQKSLTL TPTRSDSSEG FLQLPHQDSQ 1450 1460 1470 1480 1490 1500 DSSSVGSNSL EDGQTLGTKK SSMVALKRLP FLRNRPKDKD KMKACYRRSM SMNDLVQSMV 1510 1520 1530 1540 1550 1560 LAGQWTGSTE NLEVPDDIST GKRRKELGAM AFSTTAINFS TVNSSAGFRS KQLVNNKDTT 1570 1580 1590 1600 1610 1620 SFEDISPQGV SDDSSTGSRV HASRPASLDS GRTSTSNSNN NASLHEVKAG AVNNQSRPQS 1630 1640 1650 1660 1670 1680 HSSGEFSLLH DHEAWSSSGS SPIQYLKRQT RSSPVLQHKI SETLESRHHK IKTGSPGSEV 1690 1700 1710 1720 1730 1740 VTLQQFLEES NKLTSVQIKS SSQENLLDEV MKSLSVSSDF LGKDKPVSCG LARSVSGKTP 1750 1760 1770 1780 1790 1800 GDFYDRRTTK PEFLRPGPRK TEDTYFISSA GKPTPGTQGK IKLVKESSLS RQSKDSNPYA 1810 1820 1830 1840 1850 1860 TLPRASSVIS TAEGTTRRTS IHDFLTKDSR LPISVDSPPA AADSNTTAAS NVDKVQESRN 1870 SKSRSREQQS S
	Isoform 2 [UniParc]. Checksum: 32BCF709F5ADEDC0	FASTA	1,843	212,620
	10 20 30 40 50 60 MENEIFTPLL EQFMTSPLVT WVKTFGPLAA GNGTNLDEYV ALVDGVFLNQ VMLQINPKLE 70 80 90 100 110 120 SQRVNKKVNN DASLRMHNLS ILVRQIKFYY QETLQQLIMM SLPNVLIIGK NPFSEQGTEE 130 140 150 160 170 180 VKKLLLLLLG CAVQCQKKEE FIERIQGLDF DTKAAVAAHI QEVTHNQENV FDLQWMEVTD 190 200 210 220 230 240 MSQEDIEPLL KNMALHLKRL IDERDEHSET IIELSEERDG LHFLPHASSS AQSPCGSPGM 250 260 270 280 290 300 KRTESRQHLS VELADAKAKI RRLRQELEEK TEQLLDCKQE LEQMEIELKR LQQENMNLLS 310 320 330 340 350 360 DARSARMYRD ELDALREKAV RVDKLESEVS RYKERLHDIE FYKARVEELK EDNQVLLETK 370 380 390 400 410 420 TMLEDQLEGT RARSDKLHEL EKENLQLKAK LHDMEMERDM DRKKIEELME ENMTLEMAQK 430 440 450 460 470 480 QSMDESLHLG WELEQISRTS ELSEAPQKSL GHEVNELTSS RLLKLEMENQ SLTKTVEELR 490 500 510 520 530 540 TTVDSVEGNA SKILKMEKEN QRLSKKVEIL ENEIVQEKQS LQNCQNLSKD LMKEKAQLEK 550 560 570 580 590 600 TIETLRENSE RQIKILEQEN EHLNQTVSSL RQRSQISAEA RVKDIEKENK ILHESIKETS 610 620 630 640 650 660 SKLSKIEFEK RQIKKELEHY KEKGERAEEL ENELHHLEKE NELLQKKITN LKITCEKIEA 670 680 690 700 710 720 LEQENSELER ENRKLKKTLD SFKNLTFQLE SLEKENSQLD EENLELRRNV ESLKCASMKM 730 740 750 760 770 780 AQLQLENKEL ESEKEQLKKG LELLKASFKK TERLEVSYQG LDIENQRLQK TLENSNKKIQ 790 800 810 820 830 840 QLESELQDLE MENQTLQKNL EELKISSKRL EQLEKENKSL EQETSQLEKD KKQLEKENKR 850 860 870 880 890 900 LRQQAEIKDT TLEENNVKIG NLEKENKTLS KEIGIYKESC VRLKELEKEN KELVKRATID 910 920 930 940 950 960 IKTLVTLRED LVSEKLKTQQ MNNDLEKLTH ELEKIGLNKE RLLHDEQSTD DSRYKLLESK 970 980 990 1000 1010 1020 LESTLKKSLE IKEEKIAALE ARLEESTNYN QQLRQELKTV KKNYEALKQR QDEERMVQSS 1030 1040 1050 1060 1070 1080 PPISGEDNKW ERESQETTRE LLKVKDRLIE VERNNATLQA EKQALKTQLK QLETQNNNLQ 1090 1100 1110 1120 1130 1140 AQILALQRQT VSLQEQNTTL QTQNAKLQVE NSTLNSQSTS LMNQNAQLLI QQSSLENENE 1150 1160 1170 1180 1190 1200 SVIKEREDLK SLYDSLIKDH EKLELLHERQ ASEYESLISK HGTLKSAHKN LEVEHRDLED 1210 1220 1230 1240 1250 1260 RYNQLLKQKG QLEDLEKMLK VEQEKMLLEN KNHETVAAEY KKLCGENDRL NHTYSQLLKE 1270 1280 1290 1300 1310 1320 TEVLQTDHKN LKSLLNNSKL EQTRLEAEFS KLKEQYQQLD ITSTKLNNQC ELLSQLKGNL 1330 1340 1350 1360 1370 1380 EEENRHLLDQ IQTLMLQNRT LLEQNMESKD LFHVEQRQYI DKLNELRRQK EKLEEKIMDQ 1390 1400 1410 1420 1430 1440 YKFYDPSPPR RRGNWITLKM RKLIKSKKDI NRERQKSLTL TPTRSDSSEG FLQLPHQDSQ 1450 1460 1470 1480 1490 1500 DSSSVGSNSL EDGQTLGTKK SSTMNDLVQS MVLAGQWTGS TENLEVPDDI STGKRRKELG 1510 1520 1530 1540 1550 1560 AMAFSTTAIN FSTVNSSAGF RSKQLVNNKD TTSFEDISPQ GVSDDSSTGS RVHASRPASL 1570 1580 1590 1600 1610 1620 DSGRTSTSNS NNNASLHEVK AGAVNNQSRP QSHSSGEFSL LHDHEAWSSS GSSPIQYLKR 1630 1640 1650 1660 1670 1680 QTRSSPVLQH KISETLESRH HKIKTGSPGS EVVTLQQFLE ESNKLTSVQI KSSSQENLLD 1690 1700 1710 1720 1730 1740 EVMKSLSVSS DFLGKDKPVS CGLARSVSGK TPGDFYDRRT TKPEFLRPGP RKTEDTYFIS 1750 1760 1770 1780 1790 1800 SAGKPTPGTQ GKIKLVKESS LSRQSKDSNP YATLPRASSV ISTAEGTTRR TSIHDFLTKD 1810 1820 1830 1840 SRLPISVDSP PAAADSNTTA ASNVDKVQES RNSKSRSREQ QSS
	Isoform 3 [UniParc]. Checksum: 259F771ECE3DDCFD	FASTA	1,870	215,954
	10 20 30 40 50 60 MENEIFTPLL EQFMTSPLVT WVKTFGPLAA GNGTNLDEYV ALVDGVFLNQ VMLQINPKLE 70 80 90 100 110 120 SQRVNKKVNN DASLRMHNLS ILVRQIKFYY QETLQQLIMM SLPNVLIIGK NPFSEQGTEE 130 140 150 160 170 180 VKKLLLLLLG CAVQCQKKEE FIERIQGLDF DTKAAVAAHI QEVTHNQENV FDLQWMEVTD 190 200 210 220 230 240 MSQEDIEPLL KNMALHLKRL IDERDEHSET IIELSEERDG LHFLPHASSS AQSPCGSPGM 250 260 270 280 290 300 KRTESRQHLS VELADAKAKI RRLRQELEEK TEQLLDCKQE LEQMEIELKR LQQENMNLLS 310 320 330 340 350 360 DARSARMYRD ELDALREKAV RVDKLESEVS RYKERLHDIE FYKARVEELK EDNQVLLETK 370 380 390 400 410 420 TMLEDQLEGT RARSDKLHEL EKENLQLKAK LHDMEMERDM DRKKIEELME ENMTLEMAQK 430 440 450 460 470 480 QSMDESLHLG WELEQISRTS ELSEAPQKSL GHEVNELTSS RLLKLEMENQ SLTKTVEELR 490 500 510 520 530 540 TTVDSVEGNA SKILKMEKEN QRLSKKVEIL ENEIVQEKQS LQNCQNLSKD LMKEKAQLEK 550 560 570 580 590 600 TIETLRENSE RQIKILEQEN EHLNQTVSSL RQRSQISAEA RVKDIEKENK ILHESIKETS 610 620 630 640 650 660 SKLSKIEFEK RQIKKELEHY KEKGERAEEL ENELHHLEKE NELLQKKITN LKITCEKIEA 670 680 690 700 710 720 LEQENSELER ENRKLKKTLD SFKNLTFQLE SLEKENSQLD EENLELRRNV ESLKCASMKM 730 740 750 760 770 780 AQLQLENKEL ESEKEQLKKG LELLKASFKK TERLEVSYQG LDIENQRLQK TLENSNKKIQ 790 800 810 820 830 840 QLESELQDLE MENQTLQKNL EELKISSKRL EQLEKENKSL EQETSQLEKD KKQLEKENKR 850 860 870 880 890 900 LRQQAEIKDT TLEENNVKIG NLEKENKTLS KEIGIYKESC VRLKELEKEN KELVKRATID 910 920 930 940 950 960 IKTLVTLRED LVSEKLKTQQ MNNDLEKLTH ELEKIGLNKE RLLHDEQSTD DRYKLLESKL 970 980 990 1000 1010 1020 ESTLKKSLEI KEEKIAALEA RLEESTNYNQ QLRQELKTVK KNYEALKQRQ DEERMVQSSP 1030 1040 1050 1060 1070 1080 PISGEDNKWE RESQETTREL LKVKDRLIEV ERNNATLQAE KQALKTQLKQ LETQNNNLQA 1090 1100 1110 1120 1130 1140 QILALQRQTV SLQEQNTTLQ TQNAKLQVEN STLNSQSTSL MNQNAQLLIQ QSSLENENES 1150 1160 1170 1180 1190 1200 VIKEREDLKS LYDSLIKDHE KLELLHERQA SEYESLISKH GTLKSAHKNL EVEHRDLEDR 1210 1220 1230 1240 1250 1260 YNQLLKQKGQ LEDLEKMLKV EQEKMLLENK NHETVAAEYK KLCGENDRLN HTYSQLLKET 1270 1280 1290 1300 1310 1320 EVLQTDHKNL KSLLNNSKLE QTRLEAEFSK LKEQYQQLDI TSTKLNNQCE LLSQLKGNLE 1330 1340 1350 1360 1370 1380 EENRHLLDQI QTLMLQNRTL LEQNMESKDL FHVEQRQYID KLNELRRQKE KLEEKIMDQY 1390 1400 1410 1420 1430 1440 KFYDPSPPRR RGNWITLKMR KLIKSKKDIN RERQKSLTLT PTRSDSSEGF LQLPHQDSQD 1450 1460 1470 1480 1490 1500 SSSVGSNSLE DGQTLGTKKS SMVALKRLPF LRNRPKDKDK MKACYRRSMS MNDLVQSMVL 1510 1520 1530 1540 1550 1560 AGQWTGSTEN LEVPDDISTG KRRKELGAMA FSTTAINFST VNSSAGFRSK QLVNNKDTTS 1570 1580 1590 1600 1610 1620 FEDISPQGVS DDSSTGSRVH ASRPASLDSG RTSTSNSNNN ASLHEVKAGA VNNQSRPQSH 1630 1640 1650 1660 1670 1680 SSGEFSLLHD HEAWSSSGSS PIQYLKRQTR SSPVLQHKIS ETLESRHHKI KTGSPGSEVV 1690 1700 1710 1720 1730 1740 TLQQFLEESN KLTSVQIKSS SQENLLDEVM KSLSVSSDFL GKDKPVSCGL ARSVSGKTPG 1750 1760 1770 1780 1790 1800 DFYDRRTTKP EFLRPGPRKT EDTYFISSAG KPTPGTQGKI KLVKESSLSR QSKDSNPYAT 1810 1820 1830 1840 1850 1860 LPRASSVIST AEGTTRRTSI HDFLTKDSRL PISVDSPPAA ADSNTTAASN VDKVQESRNS 1870 KSRSREQQSS
	Isoform 4 [UniParc]. Checksum: 48C4D509CC7EEE68	FASTA	1,842	212,533
	10 20 30 40 50 60 MENEIFTPLL EQFMTSPLVT WVKTFGPLAA GNGTNLDEYV ALVDGVFLNQ VMLQINPKLE 70 80 90 100 110 120 SQRVNKKVNN DASLRMHNLS ILVRQIKFYY QETLQQLIMM SLPNVLIIGK NPFSEQGTEE 130 140 150 160 170 180 VKKLLLLLLG CAVQCQKKEE FIERIQGLDF DTKAAVAAHI QEVTHNQENV FDLQWMEVTD 190 200 210 220 230 240 MSQEDIEPLL KNMALHLKRL IDERDEHSET IIELSEERDG LHFLPHASSS AQSPCGSPGM 250 260 270 280 290 300 KRTESRQHLS VELADAKAKI RRLRQELEEK TEQLLDCKQE LEQMEIELKR LQQENMNLLS 310 320 330 340 350 360 DARSARMYRD ELDALREKAV RVDKLESEVS RYKERLHDIE FYKARVEELK EDNQVLLETK 370 380 390 400 410 420 TMLEDQLEGT RARSDKLHEL EKENLQLKAK LHDMEMERDM DRKKIEELME ENMTLEMAQK 430 440 450 460 470 480 QSMDESLHLG WELEQISRTS ELSEAPQKSL GHEVNELTSS RLLKLEMENQ SLTKTVEELR 490 500 510 520 530 540 TTVDSVEGNA SKILKMEKEN QRLSKKVEIL ENEIVQEKQS LQNCQNLSKD LMKEKAQLEK 550 560 570 580 590 600 TIETLRENSE RQIKILEQEN EHLNQTVSSL RQRSQISAEA RVKDIEKENK ILHESIKETS 610 620 630 640 650 660 SKLSKIEFEK RQIKKELEHY KEKGERAEEL ENELHHLEKE NELLQKKITN LKITCEKIEA 670 680 690 700 710 720 LEQENSELER ENRKLKKTLD SFKNLTFQLE SLEKENSQLD EENLELRRNV ESLKCASMKM 730 740 750 760 770 780 AQLQLENKEL ESEKEQLKKG LELLKASFKK TERLEVSYQG LDIENQRLQK TLENSNKKIQ 790 800 810 820 830 840 QLESELQDLE MENQTLQKNL EELKISSKRL EQLEKENKSL EQETSQLEKD KKQLEKENKR 850 860 870 880 890 900 LRQQAEIKDT TLEENNVKIG NLEKENKTLS KEIGIYKESC VRLKELEKEN KELVKRATID 910 920 930 940 950 960 IKTLVTLRED LVSEKLKTQQ MNNDLEKLTH ELEKIGLNKE RLLHDEQSTD DRYKLLESKL 970 980 990 1000 1010 1020 ESTLKKSLEI KEEKIAALEA RLEESTNYNQ QLRQELKTVK KNYEALKQRQ DEERMVQSSP 1030 1040 1050 1060 1070 1080 PISGEDNKWE RESQETTREL LKVKDRLIEV ERNNATLQAE KQALKTQLKQ LETQNNNLQA 1090 1100 1110 1120 1130 1140 QILALQRQTV SLQEQNTTLQ TQNAKLQVEN STLNSQSTSL MNQNAQLLIQ QSSLENENES 1150 1160 1170 1180 1190 1200 VIKEREDLKS LYDSLIKDHE KLELLHERQA SEYESLISKH GTLKSAHKNL EVEHRDLEDR 1210 1220 1230 1240 1250 1260 YNQLLKQKGQ LEDLEKMLKV EQEKMLLENK NHETVAAEYK KLCGENDRLN HTYSQLLKET 1270 1280 1290 1300 1310 1320 EVLQTDHKNL KSLLNNSKLE QTRLEAEFSK LKEQYQQLDI TSTKLNNQCE LLSQLKGNLE 1330 1340 1350 1360 1370 1380 EENRHLLDQI QTLMLQNRTL LEQNMESKDL FHVEQRQYID KLNELRRQKE KLEEKIMDQY 1390 1400 1410 1420 1430 1440 KFYDPSPPRR RGNWITLKMR KLIKSKKDIN RERQKSLTLT PTRSDSSEGF LQLPHQDSQD 1450 1460 1470 1480 1490 1500 SSSVGSNSLE DGQTLGTKKS STMNDLVQSM VLAGQWTGST ENLEVPDDIS TGKRRKELGA 1510 1520 1530 1540 1550 1560 MAFSTTAINF STVNSSAGFR SKQLVNNKDT TSFEDISPQG VSDDSSTGSR VHASRPASLD 1570 1580 1590 1600 1610 1620 SGRTSTSNSN NNASLHEVKA GAVNNQSRPQ SHSSGEFSLL HDHEAWSSSG SSPIQYLKRQ 1630 1640 1650 1660 1670 1680 TRSSPVLQHK ISETLESRHH KIKTGSPGSE VVTLQQFLEE SNKLTSVQIK SSSQENLLDE 1690 1700 1710 1720 1730 1740 VMKSLSVSSD FLGKDKPVSC GLARSVSGKT PGDFYDRRTT KPEFLRPGPR KTEDTYFISS 1750 1760 1770 1780 1790 1800 AGKPTPGTQG KIKLVKESSL SRQSKDSNPY ATLPRASSVI STAEGTTRRT SIHDFLTKDS 1810 1820 1830 1840 RLPISVDSPP AAADSNTTAA SNVDKVQESR NSKSRSREQQ SS
	Isoform 5 [UniParc]. Checksum: DE7B152EACCC2422	FASTA	1,796	207,797
	10 20 30 40 50 60 MENEIFTPLL EQFMTSPLVT WVKTFGPLAA GNGTNLDEYV ALVDGVFLNQ VMLQINPKLE 70 80 90 100 110 120 SQRVNKKVNN DASLRMHNLS ILVRQIKFYY QETLQQLIMM SLPNVLIIGK NPFSEQGTEE 130 140 150 160 170 180 VKKLLLLLLG CAVQCQKKEE FIERIQGLDF DTKAAVAAHI QEVTHNQENV FDLQWMEVTD 190 200 210 220 230 240 MSQEDIEPLL KNMALHLKRL IDERDEHSET IIELSEERDG LHFLPHASSS AQSPCGSPGM 250 260 270 280 290 300 KRTESRQHLS VELADAKAKI RRLRQELEEK TEQLLDCKQE LEQMEIELKR LQQENMNLLS 310 320 330 340 350 360 DARSARMYRD ELDALREKAV RVDKLESEVS RYKERLHDIE FYKARVEELK EDNQVLLETK 370 380 390 400 410 420 TMLEDQLEGT RARSDKLHEL EKENLQLKAK LHDMEMERDM DRKKIEELME ENMTLEMAQK 430 440 450 460 470 480 QSMDESLHLG WELEQISRTS ELSEAPQKSL GHEVNELTSS RLLKLEMENQ SLTKTVEELR 490 500 510 520 530 540 TTVDSVEGNA SKILKMEKEN QRLSKKVEIL ENEIVQEKQS LQNCQNLSKD LMKEKAQLEK 550 560 570 580 590 600 TIETLRENSE RQIKILEQEN EHLNQTVSSL RQRSQISAEA RVKDIEKENK ILHESIKETS 610 620 630 640 650 660 SKLSKIEFEK RQIKKELEHY KEKGERAEEL ENELHHLEKE NELLQKKITN LKITCEKIEA 670 680 690 700 710 720 LEQENSELER ENRKLKKTLD SFKNLTFQLE SLEKENSQLD EENLELRRNV ESLKCASMKM 730 740 750 760 770 780 AQLQLENKEL ESEKEQLKKG LELLKASFKK TERLEVSYQG LDIENQRLQK TLENSNKKIQ 790 800 810 820 830 840 QLESELQDLE MENQTLQKNL EELKISSKRL EQLEKENKSL EQETSQLEKD KKQLEKENKR 850 860 870 880 890 900 LRQQAEIKDT TLEENNVKIG NLEKENKTLS KEIGIYKESC VRLKELEKEN KELVKRATID 910 920 930 940 950 960 IKTLVTLRED LVSEKLKTQQ MNNDLEKLTH ELEKIGLNKE RLLHDEQSTD DRYKLLESKL 970 980 990 1000 1010 1020 ESTLKKSLEI KEEKIAALEA RLEESTNYNQ QLRQELKTVK KNYEALKQRQ DEERMVQSSP 1030 1040 1050 1060 1070 1080 PISGEDNKWE RESQETTREL LKVKDRLIEV ERNNATLQAE KQALKTQLKQ LETQNNNLQA 1090 1100 1110 1120 1130 1140 QILALQRQTV SLQEQNTTLQ TQNAKLQVEN STLNSQSTSL MNQNAQLLIQ QSSLENENES 1150 1160 1170 1180 1190 1200 VIKEREDLKS LYDSLIKDHE KLELLHERQA SEYESLISKH GTLKSAHKNL EVEHRDLEDR 1210 1220 1230 1240 1250 1260 YNQLLKQKGQ LEDLEKMLKV EQEKMLLENK NHETVAAEYK KLCGENDRLN HTYSQLLKET 1270 1280 1290 1300 1310 1320 EVLQTDHKNL KSLLNNSKLE QTRLEAEFSK LKEQYQQLDI TSTKLNNQCE LLSQLKGNLE 1330 1340 1350 1360 1370 1380 EENRHLLDQI QTLMLQNRTL LEQNMESKDL FHVEQRQYID KLNELRRQKE KLEEKIMDQY 1390 1400 1410 1420 1430 1440 KFYDPSPPRR RGNWITLKMR KLIKSKKDIN RERQKSLTLT PTRSDSSEGF LQLPHQDSQD 1450 1460 1470 1480 1490 1500 SSSVGSNSLE DGQTLGTKKS SMVALKRLPF LRNRPKDKDK MKACYRRSMS MNDLVQSMVL 1510 1520 1530 1540 1550 1560 AGQWTGSTEN LEVPDDISTG KRRKELGAMA FSTTAINFST VNSSAGFRSK QLVNNKDTTS 1570 1580 1590 1600 1610 1620 FEDISPQGVS DDSSTGSRVH ASRPASLDSG RTSTSNSNNN ASLHEVKAGA VNNQSRPQSH 1630 1640 1650 1660 1670 1680 SSGEFSLLHD HEAWSSSGSS PIQYLKRQTR SSPVLQHKIS ETLESRHHKI KTGSPGSEVV 1690 1700 1710 1720 1730 1740 TLQQFLEESN KLTSVQIKSS SQENLLDEVM KSLSVSSDFL GKDKPVSCGL ASVISTAEGT 1750 1760 1770 1780 1790 TRRTSIHDFL TKDSRLPISV DSPPAAADSN TTAASNVDKV QESRNSKSRS REQQSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Akt/PKB regulates actin organization and cell motility via Girdin/APE." Enomoto A., Murakami H., Asai N., Morone N., Watanabe T., Kawai K., Murakumo Y., Usukura J., Kaibuchi K., Takahashi M. Dev. Cell 9:389-402(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), FUNCTION, INTERACTION WITH AKT1, INTERACTION WITH PHOSPHOINOSITIDES, HOMOOLIGOMERIZATION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PHOSPHORYLATION AT SER-1417 BY AKT1, MUTAGENESIS OF SER-1417. Tissue: Fetal brain.
[2]	"A novel hook-related protein family and the characterization of hook-related protein 1." Simpson F., Martin S., Evans T.M., Kerr M., James D.E., Parton R.G., Teasdale R.D., Wicking C. Traffic 6:442-458(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING. Tissue: Fetal brain.
[3]	Anai M. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 5).
[6]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 811-1871 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 826-1850 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 851-1871 (ISOFORM 4). Tissue: Amygdala and Testis.
[7]	"Identification and characterization of GIV, a novel Galpha i/s-interacting protein found on COPI, endoplasmic reticulum-Golgi transport vesicles." Le-Niculescu H., Niesman I., Fischer T., DeVries L., Farquhar M.G. J. Biol. Chem. 280:22012-22020(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, SUBCELLULAR LOCATION.
[8]	"Girdin, a novel actin-binding protein, and its family of proteins possess versatile functions in the Akt and Wnt signaling pathways." Enomoto A., Ping J., Takahashi M. Ann. N. Y. Acad. Sci. 1086:169-184(2006) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1387; THR-1673 AND SER-1837, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[11]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Cervix carcinoma.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB201172 mRNA. Translation: BAE44387.1. AB125644 mRNA. Translation: BAF44475.1. AC019198 Genomic DNA. Translation: AAY14932.1. Different initiation. AC092176 Genomic DNA. Translation: AAX82029.1. AC012358 Genomic DNA. No translation available. BC132736 mRNA. Translation: AAI32737.1. BC144320 mRNA. Translation: AAI44321.1. BX537985 mRNA. Translation: CAD97945.1. Sequence problems. BX538154 mRNA. Translation: CAD98038.1. BX648138 mRNA. Translation: CAI46020.1. Different initiation.
IPI	IPI00171134. IPI00654603. IPI00892916. IPI00893288. IPI00943263.
RefSeq	NP_001129069.1. NM_001135597.1. NP_001241872.1. NM_001254943.1. NP_060554.3. NM_018084.4.
UniGene	Hs.292925.
3D structure databases
ProteinModelPortal	Q3V6T2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3V6T2. 7 interactions.
STRING	9606.ENSP00000338728.
PTM databases
PhosphoSite	Q3V6T2.
Polymorphism databases
DMDM	147644956.
Proteomic databases
PaxDb	Q3V6T2.
PRIDE	Q3V6T2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000263630; ENSP00000263630; ENSG00000115355. ENST00000336838; ENSP00000338728; ENSG00000115355. ENST00000413716; ENSP00000404431; ENSG00000115355. ENST00000436346; ENSP00000410608; ENSG00000115355.
GeneID	55704.
KEGG	hsa:55704.
UCSC	uc002ryu.2. human. uc002ryv.2. human. uc002ryw.3. human. uc010yoz.1. human. uc010ypa.1. human.
Organism-specific databases
CTD	55704.
GeneCards	GC02M055514.
HGNC	HGNC:25523. CCDC88A.
HPA	HPA038101.
MIM	609736. gene.
neXtProt	NX_Q3V6T2.
PharmGKB	PA162381751.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOVERGEN	HBG057867.
InParanoid	Q3V6T2.
OMA	TGFRSKQ.
Gene expression databases
ArrayExpress	Q3V6T2.
Bgee	Q3V6T2.
CleanEx	HS_CCDC88A.
Genevestigator	Q3V6T2.
Family and domain databases
InterPro	IPR008636. HOOK/CCDC88. [Graphical view]
Pfam	PF05622. HOOK. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CCDC88A. human.
GenomeRNAi	55704.
NextBio	60553.
SOURCE	Search...

Entry information

Entry name

GRDN_HUMAN

Accession

Primary (citable) accession number: Q3V6T2
Secondary accession number(s): A1IGE7 , B7ZM78, C9JG83, Q53SF1, Q581G3, Q5HYD0, Q7Z339, Q7Z3C5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	May 15, 2007
Last modified:	May 29, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.