Reviewed, UniProtKB/Swiss-Prot Q3V5L5 (MGT5B_HUMAN)

Names and origin

Protein names

Recommended name:
    Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B
    EC=2.4.1.155
    EC=2.4.1.-
Alternative name(s):
    Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase B
    Mannoside acetylglucosaminyltransferase 5B
    N-acetylglucosaminyl-transferase Vb
    GlcNAc-T Vb
      Short name=hGnTVb
      Short name=GNT-Vb
    N-acetylglucosaminyltransferase IX
      Short name=GNT-IX

Gene names

Name:	MGAT5B
Synonyms:	KIAA2008

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans. Catalyzes the transfer of N-acetylglucosamine (GlcNAc) to the beta 1-6 linkage of the mannose residue of GlcNAcbeta1,2-Manalpha on both the alpha1,3- and alpha1,6-linked mannose arms in the core structure of N-glycan. Also acts on the GlcNAcbeta1,2-Manalpha1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan. Plays an active role in modulating integrin and laminin-dependent adhesion and migration of neuronal cells via its activity in the O-mannosyl glycan pathway. Ref.1 Ref.2 Ref.6 Ref.7 Ref.8
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R. Ref.1 Ref.2
Cofactor	Divalent metal cations. According to some authors (Ref.2), divalent metal cations do not effect enzyme activity. Ref.1 Ref.2
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Predominantly expressed in brain. Expressed in all area of the adult and fetal brain Also expressed at much lower level in testis, spleen and thymus. Ref.1 Ref.2
Sequence similarities	Belongs to the glycosyltransferase 18 family.
Sequence caution	The sequence BAB71598.1 differs from that shown. Reason: Miscellaneous discrepancy. Chimeric sequence.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3V5L5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3V5L5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MITVNPDGKIMVRRCLVTLRPF → MALPALLTRLLPLR
     475-476: Missing.

Isoform 3 (identifier: Q3V5L5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     342-394: SNLGVPPGRG...YRCRIRVIDT → RQRRLRSSQE...TEKCPKNLVT
     395-792: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q3V5L5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-595: Missing.
     596-615: TVDYNNSEEFEAAIKAIMRT → MAGPPTAGPSPAPCVCPSLP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 792	792	Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B		PRO_0000288611
Regions
	Topological domain	1 – 24	24	Cytoplasmic Potential
	Transmembrane	25 – 45	21	Signal-anchor for type II membrane protein Potential
	Topological domain	46 – 792	747	Lumenal Potential
Amino acid modifications
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 595	595	Missing in isoform 4.		VSP_025730
	Alternative sequence	1 – 22	22	MITVN…TLRPF → MALPALLTRLLPLR in isoform 2.		VSP_025731
	Alternative sequence	342 – 394	53	SNLGV…RVIDT → RQRRLRSSQEQARCGVMGAC EKMLLEGDVASNTLLIEKPS KKETEKCPKNLVT in isoform 3.		VSP_025732
	Alternative sequence	395 – 792	398	Missing in isoform 3.		VSP_025733
	Alternative sequence	475 – 476	2	Missing in isoform 2.		VSP_025734
	Alternative sequence	596 – 615	20	TVDYN…AIMRT → MAGPPTAGPSPAPCVCPSLP in isoform 4.		VSP_025735
	Natural variant	70	1	V → I: dbSNP rs571264.		VAR_032452

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: C2EB9445FDBE9429	FASTA	792	89,535
	10 20 30 40 50 60 MITVNPDGKI MVRRCLVTLR PFRLFVLGIG FFTLCFLMTS LGGQFSARRL GDSPFTIRTE 70 80 90 100 110 120 VMGGPESRGV LRKMSDLLEL MVKRMDALAR LENSSELHRA GGDLHFPADR MPPGAGLMER 130 140 150 160 170 180 IQAIAQNVSD IAVKVDQILR HSLLLHSKVS EGRRDQCEAP SDPKFPDCSG KVEWMRARWT 190 200 210 220 230 240 SDPCYAFFGV DGTECSFLIY LSEVEWFCPP LPWRNQTAAQ RAPKPLPKVQ AVFRSNLSHL 250 260 270 280 290 300 LDLMGSGKES LIFMKKRTKR LTAQWALAAQ RLAQKLGATQ RDQKQILVHI GFLTEESGDV 310 320 330 340 350 360 FSPRVLKGGP LGEMVQWADI LTALYVLGHG LRVTVSLKEL QSNLGVPPGR GSCPLTMPLP 370 380 390 400 410 420 FDLIYTDYHG LQQMKRHMGL SFKKYRCRIR VIDTFGTEPA YNHEEYATLH GYRTNWGYWN 430 440 450 460 470 480 LNPKQFMTMF PHTPDNSFMG FVSEELNETE KRLIKGGKAS NMAVVYGKEA SIWKLQGKEK 490 500 510 520 530 540 FLGILNKYME IHGTVYYESQ RPPEVPAFVK NHGLLPQPEF QQLLRKAKLF IGFGFPYEGP 550 560 570 580 590 600 APLEAIANGC IFLQSRFSPP HSSLNHEFFR GKPTSREVFS QHPYAENFIG KPHVWTVDYN 610 620 630 640 650 660 NSEEFEAAIK AIMRTQVDPY LPYEYTCEGM LERIHAYIQH QDFCRAPDPA LPEAHAPQSP 670 680 690 700 710 720 FVLAPNATHL EWARNTSLAP GAWPPAHALR AWLAVPGRAC TDTCLDHGLI CEPSFFPFLN 730 740 750 760 770 780 SQDAFLKLQV PCDSTESEMN HLYPAFAQPG QECYLQKEPL LFSCAGSNTK YRRLCPCRDF 790 RKGQVALCQG CL
	Isoform 2. Checksum: BA3D2DDA83C56827	FASTA	782	88,312
	10 20 30 40 50 60 MALPALLTRL LPLRRLFVLG IGFFTLCFLM TSLGGQFSAR RLGDSPFTIR TEVMGGPESR 70 80 90 100 110 120 GVLRKMSDLL ELMVKRMDAL ARLENSSELH RAGGDLHFPA DRMPPGAGLM ERIQAIAQNV 130 140 150 160 170 180 SDIAVKVDQI LRHSLLLHSK VSEGRRDQCE APSDPKFPDC SGKVEWMRAR WTSDPCYAFF 190 200 210 220 230 240 GVDGTECSFL IYLSEVEWFC PPLPWRNQTA AQRAPKPLPK VQAVFRSNLS HLLDLMGSGK 250 260 270 280 290 300 ESLIFMKKRT KRLTAQWALA AQRLAQKLGA TQRDQKQILV HIGFLTEESG DVFSPRVLKG 310 320 330 340 350 360 GPLGEMVQWA DILTALYVLG HGLRVTVSLK ELQSNLGVPP GRGSCPLTMP LPFDLIYTDY 370 380 390 400 410 420 HGLQQMKRHM GLSFKKYRCR IRVIDTFGTE PAYNHEEYAT LHGYRTNWGY WNLNPKQFMT 430 440 450 460 470 480 MFPHTPDNSF MGFVSEELNE TEKRLIKGGK ASNMAVVYGK EASIWKGKEK FLGILNKYME 490 500 510 520 530 540 IHGTVYYESQ RPPEVPAFVK NHGLLPQPEF QQLLRKAKLF IGFGFPYEGP APLEAIANGC 550 560 570 580 590 600 IFLQSRFSPP HSSLNHEFFR GKPTSREVFS QHPYAENFIG KPHVWTVDYN NSEEFEAAIK 610 620 630 640 650 660 AIMRTQVDPY LPYEYTCEGM LERIHAYIQH QDFCRAPDPA LPEAHAPQSP FVLAPNATHL 670 680 690 700 710 720 EWARNTSLAP GAWPPAHALR AWLAVPGRAC TDTCLDHGLI CEPSFFPFLN SQDAFLKLQV 730 740 750 760 770 780 PCDSTESEMN HLYPAFAQPG QECYLQKEPL LFSCAGSNTK YRRLCPCRDF RKGQVALCQG CL
	Isoform 3. Checksum: 6991B4D57404E3DC	FASTA	394	44,124
	10 20 30 40 50 60 MITVNPDGKI MVRRCLVTLR PFRLFVLGIG FFTLCFLMTS LGGQFSARRL GDSPFTIRTE 70 80 90 100 110 120 VMGGPESRGV LRKMSDLLEL MVKRMDALAR LENSSELHRA GGDLHFPADR MPPGAGLMER 130 140 150 160 170 180 IQAIAQNVSD IAVKVDQILR HSLLLHSKVS EGRRDQCEAP SDPKFPDCSG KVEWMRARWT 190 200 210 220 230 240 SDPCYAFFGV DGTECSFLIY LSEVEWFCPP LPWRNQTAAQ RAPKPLPKVQ AVFRSNLSHL 250 260 270 280 290 300 LDLMGSGKES LIFMKKRTKR LTAQWALAAQ RLAQKLGATQ RDQKQILVHI GFLTEESGDV 310 320 330 340 350 360 FSPRVLKGGP LGEMVQWADI LTALYVLGHG LRVTVSLKEL QRQRRLRSSQ EQARCGVMGA 370 380 390 CEKMLLEGDV ASNTLLIEKP SKKETEKCPK NLVT
	Isoform 4. Checksum: 7C6D03389FA5352B	FASTA	197	21,672
	10 20 30 40 50 60 MAGPPTAGPS PAPCVCPSLP QVDPYLPYEY TCEGMLERIH AYIQHQDFCR APDPALPEAH 70 80 90 100 110 120 APQSPFVLAP NATHLEWARN TSLAPGAWPP AHALRAWLAV PGRACTDTCL DHGLICEPSF 130 140 150 160 170 180 FPFLNSQDAF LKLQVPCDST ESEMNHLYPA FAQPGQECYL QKEPLLFSCA GSNTKYRRLC 190 PCRDFRKGQV ALCQGCL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel beta(1,6)-N-acetylglucosaminyltransferase V (GnT-VB)." Kaneko M., Alvarez-Manilla G., Kamar M., Lee I., Lee J.-K., Troupe K., Zhang W., Osawa M., Pierce M. FEBS Lett. 554:515-519(2003) [PubMed: 14623122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ENZYME ACTIVITY, FUNCTION, COFACTOR, TISSUE SPECIFICITY.
[2]	"Molecular cloning and characterization of human GnT-IX, a novel beta1,6-N-acetylglucosaminyltransferase that is specifically expressed in the brain." Inamori K., Endo T., Ide Y., Fujii S., Gu J., Honke K., Taniguchi N. J. Biol. Chem. 278:43102-43109(2003) [PubMed: 12941944] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, FUNCTION, COFACTOR, TISSUE SPECIFICITY. Tissue: Brain.
[3]	"The nucleotide sequence of a long cDNA clone isolated from human." Nagase T., Kikuno R., Ohara O. Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4). Tissue: PNS and Skin.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 100-306. Tissue: Brain.
[6]	"N-Acetylglucosaminyltransferase IX acts on the GlcNAc beta 1,2-Man alpha 1-Ser/Thr moiety, forming a 2,6-branched structure in brain O-mannosyl glycan." Inamori K., Endo T., Gu J., Matsuo I., Ito Y., Fujii S., Iwasaki H., Narimatsu H., Miyoshi E., Honke K., Taniguchi N. J. Biol. Chem. 279:2337-2340(2004) [PubMed: 14617637] [Abstract] Cited for: FUNCTION.
[7]	"Integrin-dependent neuroblastoma cell adhesion and migration on laminin is regulated by expression levels of two enzymes in the O-mannosyl-linked glycosylation pathway, PomGnT1 and GnT-Vb." Abbott K.L., Troupe K., Lee I., Pierce M. Exp. Cell Res. 312:2837-2850(2006) [PubMed: 16857188] [Abstract] Cited for: FUNCTION.
[8]	"N-acetylglucosaminyltranferase VB expression enhances beta1 integrin-dependent PC12 neurite outgrowth on laminin and collagen." Lee I., Guo H.-B., Kamar M., Abbott K., Troupe K., Lee J.-K., Alvarez-Manilla G., Pierce M. J. Neurochem. 97:947-956(2006) [PubMed: 16606368] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB114297 mRNA. Translation: BAD02406.1. AB109185 mRNA. Translation: BAC84969.1. AB235153 mRNA. Translation: BAE44474.1. Different initiation. BC062354 mRNA. Translation: AAH62354.1. BC063862 mRNA. Translation: AAH63862.1. AK057861 mRNA. Translation: BAB71598.1. Sequence problems.
IPI	IPI00440497. IPI00441187. IPI00848338. IPI00940587.
RefSeq	NP_653278.2. NP_945193.1.
UniGene	Hs.144531
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3V5L5.
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
PTM databases
PhosphoSite	Q3V5L5.
Genome annotation databases
Ensembl	ENST00000301618; ENSP00000301618; ENSG00000167889; Homo sapiens. [Genome view] ENST00000374998; ENSP00000364137; ENSG00000167889; Homo sapiens. [Genome view] ENST00000374999; ENSP00000364138; ENSG00000167889; Homo sapiens. [Genome view] ENST00000428789; ENSP00000391227; ENSG00000167889; Homo sapiens. [Genome view] ENST00000428991; ENSP00000405263; ENSG00000167889; Homo sapiens. [Genome view] ENST00000435904; ENSP00000410905; ENSG00000167889; Homo sapiens. [Genome view]
GeneID	146664.
KEGG	hsa:146664.
UCSC	uc002jth.1. human. uc002jtj.1. human.
Organism-specific databases
CTD	146664.
GeneCards	GC17P072376.
HGNC	HGNC:24140. MGAT5B.
MIM	612441. gene.
PharmGKB	PA134987427.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q3V5L5.
OMA	KKETEKC.
Enzyme and pathway databases
BRENDA	2.4.1.155. 247.
Gene expression databases
ArrayExpress	Q3V5L5.
Bgee	Q3V5L5.
CleanEx	HS_MGAT5B.
Genevestigator	Q3V5L5.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	85408.
SOURCE	Search...

Entry information

Entry name

MGT5B_HUMAN

Accession

Primary (citable) accession number: Q3V5L5
Secondary accession number(s): Q6P3S8 , Q6P6B3, Q766X5, Q76D04, Q96LS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	November 3, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.