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Protein

Adhesion G-protein coupled receptor G5

Gene

Adgrg5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adhesion G protein-coupled receptor (GPCR). Transduces intracellular signals through coupling to guanine nucleotide-binding protein G(s) subunit alpha and activation of adenylate cyclase pathway (PubMed:22575658). Isoform 1, but not isoform 2, is constitutively active, as evidenced by elevated basal cAMP levels, and responds to mechanical activation (shaking) (PubMed:26499266).1 Publication2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Protein family/group databases

MEROPSiP02.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesion G-protein coupled receptor G5
Alternative name(s):
G-protein coupled receptor 114
G-protein coupled receptor PGR27
Gene namesi
Name:Adgrg5
Synonyms:Gm1109, Gpr114, Pgr27
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2685955. Adgrg5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 246ExtracellularCuratedAdd BLAST223
Transmembranei247 – 267Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini268 – 284CytoplasmicCuratedAdd BLAST17
Transmembranei285 – 305Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini306 – 319ExtracellularCuratedAdd BLAST14
Transmembranei320 – 340Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini341 – 351CytoplasmicCuratedAdd BLAST11
Transmembranei352 – 372Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini373 – 413ExtracellularCuratedAdd BLAST41
Transmembranei414 – 434Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini435 – 453CytoplasmicCuratedAdd BLAST19
Transmembranei454 – 476Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini477 – 480ExtracellularCurated4
Transmembranei481 – 500Helical; Name=7Sequence analysisAdd BLAST20
Topological domaini501 – 524CytoplasmicCuratedAdd BLAST24

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi222Missing : Drastic decrease in basal cAMP production. 1 Publication1
Mutagenesisi230Q → A, C, D, E, G, F, H, I, L, M, P, W or Y: Drastic reduction in basal cAMP production. 1 Publication1
Mutagenesisi230Q → K, N, R or S: No effect on basal cAMP production. 1 Publication1
Mutagenesisi230Q → T or V: Small reduction in basal cAMP production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000028863824 – 524Adhesion G-protein coupled receptor G5Add BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi65N-linked (GlcNAc...)Sequence analysis1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1
Glycosylationi143N-linked (GlcNAc...)Sequence analysis1
Glycosylationi169N-linked (GlcNAc...)Sequence analysis1
Glycosylationi175N-linked (GlcNAc...)Sequence analysis1
Glycosylationi390N-linked (GlcNAc...)Sequence analysis1
Glycosylationi396N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Autoproteolysis between residues Leu-222 and Thr-223 occurs in the lumen of the endoplasmic reticulum during receptor biosynthesis. The N-terminal fragment (NTF) subsequently reassociates with the C-terminal fragment (CTF) either in a homogeneric heterodimerization, or with another family member through heterogeneric heterodimerization. Autocatalytic cleavage is thought to be critical for the maturation, stability, trafficking, and function.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei222 – 223Cleavage; by autolysis1 Publication2

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3V3Z3.
PRIDEiQ3V3Z3.

PTM databases

PhosphoSitePlusiQ3V3Z3.

Expressioni

Tissue specificityi

Expressed at least in kidney, heart, brain and spleen. In the kidney, both isoform 1 and isoform 2 are expressed at similar levels. Isoform 1 is predominant in spleen, while isoform 2 is the major form in heart and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000061577.
CleanExiMM_GPR114.
ExpressionAtlasiQ3V3Z3. baseline and differential.
GenevisibleiQ3V3Z3. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132628.

Structurei

3D structure databases

ProteinModelPortaliQ3V3Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini182 – 235GPSPROSITE-ProRule annotationAdd BLAST54

Sequence similaritiesi

Contains 1 GPS domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOVERGENiHBG107961.
InParanoidiQ3V3Z3.
KOiK08459.
TreeFamiTF321769.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR003910. GPR1/GPR3/GPR5.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01422. GPR56ORPHANR.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3V3Z3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPHGALFFY LCLLAAQVVL VETLSDLLVL MKRLEQPVGR GLSSRARHIH
60 70 80 90 100
SLEQKLLNAS FGGHNLTLQT NSIQSLVFKL SCDFPGLSLS STTLTNVSQV
110 120 130 140 150
RAPHAMQFPA ELTKGACVTS RPAELRLICI YFFTAHLFQD DRNSSLLNNY
160 170 180 190 200
VLGAQLDHRP VNNLQKPVNI SFWHNRSLEG YTVSCVFWKE GASKSSWGAW
210 220 230 240 250
SPEGCYTEQP SATQVLCHCN HLTYFAVLMQ LSGDPVPAEL QVPLEYISFV
260 270 280 290 300
GCSISIVASL LTILLYAQSR KQSDSTTRIH MNLNGSVLLL NVTFLLSSQM
310 320 330 340 350
TLPTMPRPVC KVLAAVLHYA LLSSLTWMAI EGFNLYLFLG RVYNAYIRRY
360 370 380 390 400
LLKLCMLGWG FPALLVLLLL MIKSSVYGPC VTSLSKSQEN GTGFQNVSMC
410 420 430 440 450
WIRSPMVHSI LVMGYGGFTS LFNLVVLAWA LWILCRLRAR EKALSPWAYR
460 470 480 490 500
DTAMVLGLTV LLGTTWTLAF FSFGVFLLPQ LFLFTIFNSL YGFFLFLWFC
510 520
SQKRYSDAEA KAEMEAVSSS QMTH
Length:524
Mass (Da):58,675
Last modified:October 5, 2016 - v3
Checksum:iE1AE9C85BA11DDC7
GO
Isoform 2 (identifier: Q3V3Z3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     230-230: Missing.

Note: May be due to competing acceptor splice site.
Show »
Length:523
Mass (Da):58,547
Checksum:i330D435A94E0894C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti79K → N in BAE20445 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058541230Missing in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028878 mRNA. Translation: BAE20445.1.
AC129606 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11153.1.
BC144851 mRNA. Translation: AAI44852.1.
BC145803 mRNA. Translation: AAI45804.1.
CCDSiCCDS22552.1. [Q3V3Z3-2]
CCDS52637.1. [Q3V3Z3-1]
RefSeqiNP_001028640.2. NM_001033468.3. [Q3V3Z3-2]
NP_001139444.1. NM_001145972.1. [Q3V3Z3-1]
XP_011246724.1. XM_011248422.1. [Q3V3Z3-2]
XP_017168373.1. XM_017312884.1. [Q3V3Z3-1]
XP_017168374.1. XM_017312885.1. [Q3V3Z3-1]
XP_017168375.1. XM_017312886.1. [Q3V3Z3-1]
UniGeneiMm.334726.

Genome annotation databases

EnsembliENSMUST00000074570; ENSMUSP00000074155; ENSMUSG00000061577. [Q3V3Z3-2]
ENSMUST00000166802; ENSMUSP00000132628; ENSMUSG00000061577. [Q3V3Z3-1]
GeneIDi382045.
KEGGimmu:382045.
UCSCiuc009mxi.2. mouse. [Q3V3Z3-1]
uc009mxj.2. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028878 mRNA. Translation: BAE20445.1.
AC129606 Genomic DNA. No translation available.
CH466525 Genomic DNA. Translation: EDL11153.1.
BC144851 mRNA. Translation: AAI44852.1.
BC145803 mRNA. Translation: AAI45804.1.
CCDSiCCDS22552.1. [Q3V3Z3-2]
CCDS52637.1. [Q3V3Z3-1]
RefSeqiNP_001028640.2. NM_001033468.3. [Q3V3Z3-2]
NP_001139444.1. NM_001145972.1. [Q3V3Z3-1]
XP_011246724.1. XM_011248422.1. [Q3V3Z3-2]
XP_017168373.1. XM_017312884.1. [Q3V3Z3-1]
XP_017168374.1. XM_017312885.1. [Q3V3Z3-1]
XP_017168375.1. XM_017312886.1. [Q3V3Z3-1]
UniGeneiMm.334726.

3D structure databases

ProteinModelPortaliQ3V3Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000132628.

Protein family/group databases

MEROPSiP02.016.
GPCRDBiSearch...

PTM databases

PhosphoSitePlusiQ3V3Z3.

Proteomic databases

PaxDbiQ3V3Z3.
PRIDEiQ3V3Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074570; ENSMUSP00000074155; ENSMUSG00000061577. [Q3V3Z3-2]
ENSMUST00000166802; ENSMUSP00000132628; ENSMUSG00000061577. [Q3V3Z3-1]
GeneIDi382045.
KEGGimmu:382045.
UCSCiuc009mxi.2. mouse. [Q3V3Z3-1]
uc009mxj.2. mouse.

Organism-specific databases

CTDi221188.
MGIiMGI:2685955. Adgrg5.

Phylogenomic databases

eggNOGiKOG4193. Eukaryota.
ENOG410XSD2. LUCA.
GeneTreeiENSGT00830000128227.
HOVERGENiHBG107961.
InParanoidiQ3V3Z3.
KOiK08459.
TreeFamiTF321769.

Miscellaneous databases

PROiQ3V3Z3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061577.
CleanExiMM_GPR114.
ExpressionAtlasiQ3V3Z3. baseline and differential.
GenevisibleiQ3V3Z3. MM.

Family and domain databases

InterProiIPR017981. GPCR_2-like.
IPR000832. GPCR_2_secretin-like.
IPR003910. GPR1/GPR3/GPR5.
IPR000203. GPS.
[Graphical view]
PfamiPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
[Graphical view]
PRINTSiPR00249. GPCRSECRETIN.
PR01422. GPR56ORPHANR.
SMARTiSM00303. GPS. 1 hit.
[Graphical view]
PROSITEiPS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAGRG5_MOUSE
AccessioniPrimary (citable) accession number: Q3V3Z3
Secondary accession number(s): A6H6A1, G5E8G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 5, 2016
Last modified: November 30, 2016
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.