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Protein

Ubiquitin-associated and SH3 domain-containing protein A

Gene

Ubash3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain (By similarity). Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation.By similarity1 Publication

GO - Biological processi

  • negative regulation of T cell receptor signaling pathway Source: MGI
  • regulation of cytokine production Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-associated and SH3 domain-containing protein A
Alternative name(s):
Suppressor of T-cell receptor signaling 2
Short name:
STS-2
T-cell ubiquitin ligand 1
Short name:
TULA-1
Gene namesi
Name:Ubash3a
ORF Names:Sts2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1926074. Ubash3a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004153721 – 624Ubiquitin-associated and SH3 domain-containing protein AAdd BLAST624

Proteomic databases

EPDiQ3V3E1.
MaxQBiQ3V3E1.
PaxDbiQ3V3E1.
PRIDEiQ3V3E1.

PTM databases

PhosphoSitePlusiQ3V3E1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042345.
ExpressionAtlasiQ3V3E1. baseline and differential.
GenevisibleiQ3V3E1. MM.

Interactioni

Subunit structurei

Homodimer or homooligomer. Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins. Interacts with dynamin (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045890.

Structurei

Secondary structure

1624
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi360 – 365Combined sources6
Helixi370 – 374Combined sources5
Helixi378 – 381Combined sources4
Beta strandi385 – 387Combined sources3
Helixi406 – 412Combined sources7
Helixi418 – 434Combined sources17
Beta strandi438 – 443Combined sources6
Helixi447 – 459Combined sources13
Turni463 – 465Combined sources3
Beta strandi468 – 470Combined sources3
Helixi472 – 474Combined sources3
Helixi478 – 480Combined sources3
Helixi485 – 488Combined sources4
Helixi492 – 497Combined sources6
Helixi512 – 514Combined sources3
Helixi521 – 536Combined sources16
Beta strandi544 – 550Combined sources7
Helixi554 – 557Combined sources4
Helixi560 – 563Combined sources4
Helixi570 – 578Combined sources9
Beta strandi585 – 590Combined sources6
Turni592 – 594Combined sources3
Beta strandi597 – 600Combined sources4
Helixi617 – 620Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D4IX-ray1.95A/B/C/D354-622[»]
3D6AX-ray2.25A/B/C/D354-622[»]
3DB1X-ray2.77A/B/C/D354-622[»]
ProteinModelPortaliQ3V3E1.
SMRiQ3V3E1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3V3E1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 60UBAPROSITE-ProRule annotationAdd BLAST42
Domaini238 – 303SH3PROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni358 – 624Phosphatase-likeAdd BLAST267

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ3V3E1.
KOiK18993.
OMAiTLTHGSN.
OrthoDBiEOG091G0DJ2.
PhylomeDBiQ3V3E1.
TreeFamiTF313334.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 2 hits.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3V3E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGETQLYA KVSNKLKGRS TPSLLDPLLA MGFPTHTALK ALAATGRKTA
60 70 80 90 100
EAAADWLHGH CNDPSLDDPI PQEYALFLCP TGPLLEKLQE FWRESRRQCA
110 120 130 140 150
KNRAHEVFPH VTLCDFFTCE DQKVECLYEA LRRAGDRILG SFPTLVPLVL
160 170 180 190 200
HSSISYLGFF INDSPADAIR EFAMAFATEA AVLADCTIKP CTKQLHLTLA
210 220 230 240 250
HKFYPHHQRT LEQLAKAIQP SHSCQWTAAL YSRDMRFVHY QTLKALFQYK
260 270 280 290 300
PQNADELMLS AGDYIFVDPT QQEEASEGWA IGISHRTGCR GFLPENYTER
310 320 330 340 350
ANEADTWVKH RTYTFNLAMD LNSRKDFEAS CRGNGEPHTP SMSKSVSSIQ
360 370 380 390 400
ALQATISRRG ILVVRHGERV DQVFGKSWLQ QCTTADGKYY RPDLNFPRSL
410 420 430 440 450
PRRSNGIKDF ENDPPLSSCG IFQARLAGEA LLDSGVRVTA VFASPALRCV
460 470 480 490 500
QTAKHILEEL KLEKKLKIRV EPGIFEWMKW EASKATLTFL TLEELKEANF
510 520 530 540 550
NVDLDYRPAL PRCSLMPAES YDQYVERCAV SMGQIINTCP QDMGITLIVS
560 570 580 590 600
HSSALDSCTR PLLGLPPREC GDFAQLVRKI PSLGMCFCEE NREDGKWDLV
610 620
NPPVKTLTHG ANSVFNWRNW ISSN
Length:624
Mass (Da):70,145
Last modified:October 11, 2005 - v1
Checksum:iE40AFB541AC84538
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75A → V in BAE41311 (PubMed:16141072).Curated1
Sequence conflicti331C → S in BAE41311 (PubMed:16141072).Curated1
Sequence conflicti337P → S in BAE41311 (PubMed:16141072).Curated1
Sequence conflicti444S → F in BAE41311 (PubMed:16141072).Curated1
Sequence conflicti463E → D in BAE41311 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041690 mRNA. Translation: BAE20600.1.
AK169696 mRNA. Translation: BAE41311.1.
CU024900, AC154652, AC167247 Genomic DNA. Translation: CAX15943.1.
CCDSiCCDS50054.1.
RefSeqiNP_808491.2. NM_177823.4.
UniGeneiMm.387930.

Genome annotation databases

EnsembliENSMUST00000048656; ENSMUSP00000045890; ENSMUSG00000042345.
GeneIDi328795.
KEGGimmu:328795.
UCSCiuc008bus.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041690 mRNA. Translation: BAE20600.1.
AK169696 mRNA. Translation: BAE41311.1.
CU024900, AC154652, AC167247 Genomic DNA. Translation: CAX15943.1.
CCDSiCCDS50054.1.
RefSeqiNP_808491.2. NM_177823.4.
UniGeneiMm.387930.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D4IX-ray1.95A/B/C/D354-622[»]
3D6AX-ray2.25A/B/C/D354-622[»]
3DB1X-ray2.77A/B/C/D354-622[»]
ProteinModelPortaliQ3V3E1.
SMRiQ3V3E1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045890.

PTM databases

PhosphoSitePlusiQ3V3E1.

Proteomic databases

EPDiQ3V3E1.
MaxQBiQ3V3E1.
PaxDbiQ3V3E1.
PRIDEiQ3V3E1.

Protocols and materials databases

DNASUi328795.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048656; ENSMUSP00000045890; ENSMUSG00000042345.
GeneIDi328795.
KEGGimmu:328795.
UCSCiuc008bus.2. mouse.

Organism-specific databases

CTDi53347.
MGIiMGI:1926074. Ubash3a.

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ3V3E1.
KOiK18993.
OMAiTLTHGSN.
OrthoDBiEOG091G0DJ2.
PhylomeDBiQ3V3E1.
TreeFamiTF313334.

Miscellaneous databases

EvolutionaryTraceiQ3V3E1.
PROiQ3V3E1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042345.
ExpressionAtlasiQ3V3E1. baseline and differential.
GenevisibleiQ3V3E1. MM.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 2 hits.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBS3A_MOUSE
AccessioniPrimary (citable) accession number: Q3V3E1
Secondary accession number(s): Q3TED7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Has very nearly lost all enzyme activity. Has very low, but measurable tyrosine phosphatase activity at pH 5.0.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.