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Protein

Ubiquitin-associated and SH3 domain-containing protein A

Gene

Ubash3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain (By similarity). Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation.By similarity1 Publication

GO - Biological processi

  • negative regulation of T cell receptor signaling pathway Source: MGI
  • regulation of cytokine production Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-associated and SH3 domain-containing protein A
Alternative name(s):
Suppressor of T-cell receptor signaling 2
Short name:
STS-2
T-cell ubiquitin ligand 1
Short name:
TULA-1
Gene namesi
Name:Ubash3a
ORF Names:Sts2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1926074. Ubash3a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Ubiquitin-associated and SH3 domain-containing protein APRO_0000415372Add
BLAST

Proteomic databases

EPDiQ3V3E1.
MaxQBiQ3V3E1.
PaxDbiQ3V3E1.
PRIDEiQ3V3E1.

PTM databases

PhosphoSiteiQ3V3E1.

Expressioni

Gene expression databases

BgeeiQ3V3E1.
ExpressionAtlasiQ3V3E1. baseline and differential.
GenevisibleiQ3V3E1. MM.

Interactioni

Subunit structurei

Homodimer or homooligomer. Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins. Interacts with dynamin (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045890.

Structurei

Secondary structure

1
624
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi360 – 3656Combined sources
Helixi370 – 3745Combined sources
Helixi378 – 3814Combined sources
Beta strandi385 – 3873Combined sources
Helixi406 – 4127Combined sources
Helixi418 – 43417Combined sources
Beta strandi438 – 4436Combined sources
Helixi447 – 45913Combined sources
Turni463 – 4653Combined sources
Beta strandi468 – 4703Combined sources
Helixi472 – 4743Combined sources
Helixi478 – 4803Combined sources
Helixi485 – 4884Combined sources
Helixi492 – 4976Combined sources
Helixi512 – 5143Combined sources
Helixi521 – 53616Combined sources
Beta strandi544 – 5507Combined sources
Helixi554 – 5574Combined sources
Helixi560 – 5634Combined sources
Helixi570 – 5789Combined sources
Beta strandi585 – 5906Combined sources
Turni592 – 5943Combined sources
Beta strandi597 – 6004Combined sources
Helixi617 – 6204Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D4IX-ray1.95A/B/C/D354-622[»]
3D6AX-ray2.25A/B/C/D354-622[»]
3DB1X-ray2.77A/B/C/D354-622[»]
ProteinModelPortaliQ3V3E1.
SMRiQ3V3E1. Positions 24-70, 233-310, 354-622.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3V3E1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 6042UBAPROSITE-ProRule annotationAdd
BLAST
Domaini238 – 30366SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni358 – 624267Phosphatase-likeAdd
BLAST

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ3V3E1.
KOiK18993.
OMAiTLTHGSN.
OrthoDBiEOG7P5T0D.
PhylomeDBiQ3V3E1.
TreeFamiTF313334.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3V3E1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGETQLYA KVSNKLKGRS TPSLLDPLLA MGFPTHTALK ALAATGRKTA
60 70 80 90 100
EAAADWLHGH CNDPSLDDPI PQEYALFLCP TGPLLEKLQE FWRESRRQCA
110 120 130 140 150
KNRAHEVFPH VTLCDFFTCE DQKVECLYEA LRRAGDRILG SFPTLVPLVL
160 170 180 190 200
HSSISYLGFF INDSPADAIR EFAMAFATEA AVLADCTIKP CTKQLHLTLA
210 220 230 240 250
HKFYPHHQRT LEQLAKAIQP SHSCQWTAAL YSRDMRFVHY QTLKALFQYK
260 270 280 290 300
PQNADELMLS AGDYIFVDPT QQEEASEGWA IGISHRTGCR GFLPENYTER
310 320 330 340 350
ANEADTWVKH RTYTFNLAMD LNSRKDFEAS CRGNGEPHTP SMSKSVSSIQ
360 370 380 390 400
ALQATISRRG ILVVRHGERV DQVFGKSWLQ QCTTADGKYY RPDLNFPRSL
410 420 430 440 450
PRRSNGIKDF ENDPPLSSCG IFQARLAGEA LLDSGVRVTA VFASPALRCV
460 470 480 490 500
QTAKHILEEL KLEKKLKIRV EPGIFEWMKW EASKATLTFL TLEELKEANF
510 520 530 540 550
NVDLDYRPAL PRCSLMPAES YDQYVERCAV SMGQIINTCP QDMGITLIVS
560 570 580 590 600
HSSALDSCTR PLLGLPPREC GDFAQLVRKI PSLGMCFCEE NREDGKWDLV
610 620
NPPVKTLTHG ANSVFNWRNW ISSN
Length:624
Mass (Da):70,145
Last modified:October 11, 2005 - v1
Checksum:iE40AFB541AC84538
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751A → V in BAE41311 (PubMed:16141072).Curated
Sequence conflicti331 – 3311C → S in BAE41311 (PubMed:16141072).Curated
Sequence conflicti337 – 3371P → S in BAE41311 (PubMed:16141072).Curated
Sequence conflicti444 – 4441S → F in BAE41311 (PubMed:16141072).Curated
Sequence conflicti463 – 4631E → D in BAE41311 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041690 mRNA. Translation: BAE20600.1.
AK169696 mRNA. Translation: BAE41311.1.
CU024900, AC154652, AC167247 Genomic DNA. Translation: CAX15943.1.
CCDSiCCDS50054.1.
RefSeqiNP_808491.2. NM_177823.4.
UniGeneiMm.387930.

Genome annotation databases

EnsembliENSMUST00000048656; ENSMUSP00000045890; ENSMUSG00000042345.
GeneIDi328795.
KEGGimmu:328795.
UCSCiuc008bus.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041690 mRNA. Translation: BAE20600.1.
AK169696 mRNA. Translation: BAE41311.1.
CU024900, AC154652, AC167247 Genomic DNA. Translation: CAX15943.1.
CCDSiCCDS50054.1.
RefSeqiNP_808491.2. NM_177823.4.
UniGeneiMm.387930.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3D4IX-ray1.95A/B/C/D354-622[»]
3D6AX-ray2.25A/B/C/D354-622[»]
3DB1X-ray2.77A/B/C/D354-622[»]
ProteinModelPortaliQ3V3E1.
SMRiQ3V3E1. Positions 24-70, 233-310, 354-622.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045890.

PTM databases

PhosphoSiteiQ3V3E1.

Proteomic databases

EPDiQ3V3E1.
MaxQBiQ3V3E1.
PaxDbiQ3V3E1.
PRIDEiQ3V3E1.

Protocols and materials databases

DNASUi328795.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048656; ENSMUSP00000045890; ENSMUSG00000042345.
GeneIDi328795.
KEGGimmu:328795.
UCSCiuc008bus.2. mouse.

Organism-specific databases

CTDi53347.
MGIiMGI:1926074. Ubash3a.

Phylogenomic databases

eggNOGiKOG3734. Eukaryota.
ENOG410YGUZ. LUCA.
GeneTreeiENSGT00390000018249.
HOGENOMiHOG000012936.
HOVERGENiHBG018025.
InParanoidiQ3V3E1.
KOiK18993.
OMAiTLTHGSN.
OrthoDBiEOG7P5T0D.
PhylomeDBiQ3V3E1.
TreeFamiTF313334.

Miscellaneous databases

EvolutionaryTraceiQ3V3E1.
PROiQ3V3E1.
SOURCEiSearch...

Gene expression databases

BgeeiQ3V3E1.
ExpressionAtlasiQ3V3E1. baseline and differential.
GenevisibleiQ3V3E1. MM.

Family and domain databases

Gene3Di3.40.50.1240. 2 hits.
3.90.1140.10. 1 hit.
InterProiIPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
IPR001452. SH3_domain.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF00300. His_Phos_1. 1 hit.
PF14604. SH3_9. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF46934. SSF46934. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF53254. SSF53254. 2 hits.
PROSITEiPS50002. SH3. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Thymus.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "Determination of the substrate specificity of protein-tyrosine phosphatase TULA-2 and identification of Syk as a TULA-2 substrate."
    Chen X., Ren L., Kim S., Carpino N., Daniel J.L., Kunapuli S.P., Tsygankov A.Y., Pei D.
    J. Biol. Chem. 285:31268-31276(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: LACK OF PROTEIN PHOSPHATASE ACTIVITY.
  4. "Structural and functional characterization of the 2H-phosphatase domain of Sts-2 reveals an acid-dependent phosphatase activity."
    Chen Y., Jakoncic J., Carpino N., Nassar N.
    Biochemistry 48:1681-1690(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 354-622, FUNCTION, VERY LOW PHOSPHATASE ACTIVITY AT PH 5.

Entry informationi

Entry nameiUBS3A_MOUSE
AccessioniPrimary (citable) accession number: Q3V3E1
Secondary accession number(s): Q3TED7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Has very nearly lost all enzyme activity. Has very low, but measurable tyrosine phosphatase activity at pH 5.0.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.