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Q3V1T4 (P3H1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prolyl 3-hydroxylase 1

EC=1.14.11.7
Alternative name(s):
Growth suppressor 1
Leucine- and proline-enriched proteoglycan 1
Short name=Leprecan-1
Gene names
Name:Lepre1
Synonyms:Gros1, P3h1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length739 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts By similarity.

Catalytic activity

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactor

Iron By similarity.

Ascorbate By similarity.

Subcellular location

Endoplasmic reticulum By similarity. Secretedextracellular spaceextracellular matrix By similarity. Note: Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan (CSPG).

Post-translational modification

O-glycosylated; chondroitin sulfate By similarity.

Sequence similarities

Belongs to the leprecan family.

Contains 1 Fe2OG dioxygenase domain.

Contains 4 TPR repeats.

Sequence caution

The sequence BAB27041.1 differs from that shown. Reason: Frameshift at position 707.

The sequence BAE21065.1 differs from that shown. Reason: Intron retention.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3V1T4-1)

Also known as: GROS1-L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3V1T4-2)

Also known as: GROS1-S;

The sequence of this isoform differs from the canonical sequence as follows:
     540-543: MYYN → TALQ
     544-739: Missing.
Isoform 3 (identifier: Q3V1T4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 739714Prolyl 3-hydroxylase 1
PRO_0000240353

Regions

Repeat36 – 6934TPR 1
Repeat146 – 17934TPR 2
Repeat208 – 24134TPR 3
Repeat304 – 33734TPR 4
Domain567 – 681115Fe2OG dioxygenase
Coiled coil404 – 44239 Potential
Motif736 – 7394Prevents secretion from ER Potential

Sites

Active site6721 By similarity
Metal binding5901Iron
Metal binding5921Iron
Metal binding6621Iron

Amino acid modifications

Glycosylation3191N-linked (GlcNAc...) Potential
Glycosylation4701N-linked (GlcNAc...) Potential
Glycosylation5431N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence1 – 179179Missing in isoform 3.
VSP_019349
Alternative sequence540 – 5434MYYN → TALQ in isoform 2.
VSP_019350
Alternative sequence544 – 739196Missing in isoform 2.
VSP_019351

Experimental info

Sequence conflict4 – 2522SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04806. Ref.1
Sequence conflict4 – 2522SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04807. Ref.1
Sequence conflict501G → R in AAF04806. Ref.1
Sequence conflict501G → R in AAF04807. Ref.1
Sequence conflict371 – 3722RS → PN in AAF04806. Ref.1
Sequence conflict371 – 3722RS → PN in AAF04807. Ref.1
Sequence conflict4031P → T in BAE21065. Ref.2
Sequence conflict4201Missing in BAC26962. Ref.2
Sequence conflict4841D → N in BAE35138. Ref.2
Sequence conflict5691L → F in AAF04806. Ref.1
Sequence conflict5891V → D in BAE21065. Ref.2
Sequence conflict6011L → F in AAF04806. Ref.1
Sequence conflict6141D → E in AAF04806. Ref.1
Sequence conflict6851H → Q in BAC26962. Ref.2
Sequence conflict7161D → G in BAE35138. Ref.2
Sequence conflict7161D → G in AAH24047. Ref.3
Sequence conflict727 – 73913SLSDR…HKDEL → FLHGATVLGVGIA in AAF04806. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (GROS1-L) [UniParc].

Last modified June 27, 2006. Version 2.
Checksum: 3484AE68E80B68E8

FASTA73983,651
        10         20         30         40         50         60 
MAVSERRLLA AMLAVAAAAA LRVAAESEPG WDVAAPDLLY AEGTAAYSRG DWPGVVLNME 

        70         80         90        100        110        120 
RALRSRAALR ALRLRCRTRC ATELPWAPDL DLGPDPSLSQ DPGAAALHDL RFFGAVLRRA 

       130        140        150        160        170        180 
ACLRRCLGPP SAHLLSEELD LEFNKRSPYN YLQVAYFKIN KLEKAVAAAH TFFVGNPEHM 

       190        200        210        220        230        240 
EMRQNLDYYQ TMSGVKEADF RDLEAKPHMH EFRLGVRLYS EEKPQEAVPH LEAALQEYFV 

       250        260        270        280        290        300 
ADEECRALCE GPYDYDGYNY LDYSADLFQA ITDHYVQVLN CKQNCVTELA SHPSREKPFE 

       310        320        330        340        350        360 
DFLPSHYNYL QFAYYNIGNY TQAIECAKTY LLFFPNDEVM HQNLAYYTAM LGEEEASSIS 

       370        380        390        400        410        420 
PRENAEEYRR RSLLEKELLF FAYDIFGIPF VDPDSWTPEE VIPKRLQEKQ KSERETAVRI 

       430        440        450        460        470        480 
SQEIGNLMKE IETLVEEKTK ESLDVSRLTR EGGPLLYEGI SLTMNSKVLN GSQRVVMDGV 

       490        500        510        520        530        540 
ISDDECQELQ RLTNAAATSG DGYRGQTSPH TPNEKFYGVT VLKALKLGQE GKVPLQSARM 

       550        560        570        580        590        600 
YYNVTEKVRR VMESYFRLDT PLYFSYSHLV CRTAIEESQA ERKDSSHPVH VDNCILNAEA 

       610        620        630        640        650        660 
LMCIKEPPAY TFRDYSAILY LNGDFDGGNF YFTELDAKTV TAEVQPQCGR AVGFSSGTEN 

       670        680        690        700        710        720 
PHGVKAVTRG QRCAIALWFT LDPRHSERDR VQADDLVKML FSPEEVDLPQ EQPLPDQQGS 

       730 
PEPGEESLSD RGSLHKDEL 

« Hide

Isoform 2 (GROS1-S) [UniParc].

Checksum: 2166EB32EC38A766
Show »

FASTA54361,397
Isoform 3 [UniParc].

Checksum: 673B7643F80A1388
Show »

FASTA56064,102

References

« Hide 'large scale' references
[1]"Gros1, a potential growth suppressor on chromosome 1: its identity to basement membrane-associated proteoglycan, leprecan."
Kaul S.C., Sugihara T., Yoshida A., Nomura H., Wadhwa R.
Oncogene 19:3576-3583(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Strain: CD-1/ICR.
Tissue: Fibroblast and Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
Strain: C57BL/6J.
Tissue: Embryonic stem cell, Pituitary and Placenta.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF165163 mRNA. Translation: AAF04806.1. Sequence problems.
AF165164 mRNA. Translation: AAF04807.1. Sequence problems.
AK010578 mRNA. Translation: BAB27041.1. Frameshift.
AK030436 mRNA. Translation: BAC26962.1.
AK132262 mRNA. Translation: BAE21065.1. Sequence problems.
AK159505 mRNA. Translation: BAE35138.1.
AL606975 Genomic DNA. Translation: CAM21645.1.
AL606975 Genomic DNA. Translation: CAO78098.1.
BC024047 mRNA. Translation: AAH24047.1.
CCDSCCDS38859.1. [Q3V1T4-1]
CCDS38860.1. [Q3V1T4-3]
RefSeqNP_001035874.1. NM_001042411.1. [Q3V1T4-3]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1]
UniGeneMm.27961.

3D structure databases

ProteinModelPortalQ3V1T4.
SMRQ3V1T4. Positions 34-65.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ3V1T4. 1 interaction.
MINTMINT-4125499.

PTM databases

PhosphoSiteQ3V1T4.

Proteomic databases

PaxDbQ3V1T4.
PRIDEQ3V1T4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641. [Q3V1T4-3]
ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641. [Q3V1T4-1]
GeneID56401.
KEGGmmu:56401.
UCSCuc008ulq.1. mouse. [Q3V1T4-1]

Organism-specific databases

CTD64175.
MGIMGI:1888921. Lepre1.

Phylogenomic databases

eggNOGNOG269251.
GeneTreeENSGT00550000074573.
HOVERGENHBG053224.
KOK08134.
OrthoDBEOG7BZVSS.

Gene expression databases

ArrayExpressQ3V1T4.
BgeeQ3V1T4.
CleanExMM_LEPRE1.
GenevestigatorQ3V1T4.

Family and domain databases

Gene3D1.25.40.10. 3 hits.
InterProIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
[Graphical view]
PfamPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio312512.
PROQ3V1T4.
SOURCESearch...

Entry information

Entry nameP3H1_MOUSE
AccessionPrimary (citable) accession number: Q3V1T4
Secondary accession number(s): A2A7Q4 expand/collapse secondary AC list , A6PW85, Q3TWX8, Q8BSV2, Q8CFL3, Q9CWK5, Q9QZT6, Q9QZT7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot