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Protein

Prolyl 3-hydroxylase 1

Gene

Lepre1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts (By similarity).By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi590 – 5901Iron
Metal bindingi592 – 5921Iron
Metal bindingi662 – 6621Iron
Active sitei672 – 6721By similarity

GO - Molecular functioni

GO - Biological processi

  • bone development Source: MGI
  • cell growth Source: MGI
  • collagen fibril organization Source: MGI
  • collagen metabolic process Source: MGI
  • negative regulation of post-translational protein modification Source: MGI
  • peptidyl-proline hydroxylation Source: GOC
  • protein folding Source: MGI
  • protein hydroxylation Source: MGI
  • protein stabilization Source: MGI
  • regulation of ossification Source: MGI
  • regulation of protein secretion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.
ReactomeiREACT_285754. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 1 (EC:1.14.11.7)
Alternative name(s):
Growth suppressor 1
Leucine- and proline-enriched proteoglycan 1
Short name:
Leprecan-1
Gene namesi
Name:Lepre1
Synonyms:Gros1, P3h1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1888921. Lepre1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 739714Prolyl 3-hydroxylase 1PRO_0000240353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi319 – 3191N-linked (GlcNAc...)Sequence Analysis
Glycosylationi470 – 4701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi543 – 5431N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

O-glycosylated; chondroitin sulfate.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ3V1T4.
PaxDbiQ3V1T4.
PRIDEiQ3V1T4.

PTM databases

PhosphoSiteiQ3V1T4.

Expressioni

Gene expression databases

BgeeiQ3V1T4.
CleanExiMM_LEPRE1.
ExpressionAtlasiQ3V1T4. baseline and differential.
GenevisibleiQ3V1T4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ3V1T4. 1 interaction.
MINTiMINT-4125499.
STRINGi10090.ENSMUSP00000099723.

Structurei

3D structure databases

ProteinModelPortaliQ3V1T4.
SMRiQ3V1T4. Positions 152-188, 210-236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati36 – 6934TPR 1Add
BLAST
Repeati146 – 17934TPR 2Add
BLAST
Repeati208 – 24134TPR 3Add
BLAST
Repeati304 – 33734TPR 4Add
BLAST
Domaini567 – 681115Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili404 – 44239Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 7394Prevents secretion from ERPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOVERGENiHBG053224.
InParanoidiQ3V1T4.
KOiK08134.
OrthoDBiEOG7BZVSS.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3V1T4-1) [UniParc]FASTAAdd to basket

Also known as: GROS1-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVSERRLLA AMLAVAAAAA LRVAAESEPG WDVAAPDLLY AEGTAAYSRG
60 70 80 90 100
DWPGVVLNME RALRSRAALR ALRLRCRTRC ATELPWAPDL DLGPDPSLSQ
110 120 130 140 150
DPGAAALHDL RFFGAVLRRA ACLRRCLGPP SAHLLSEELD LEFNKRSPYN
160 170 180 190 200
YLQVAYFKIN KLEKAVAAAH TFFVGNPEHM EMRQNLDYYQ TMSGVKEADF
210 220 230 240 250
RDLEAKPHMH EFRLGVRLYS EEKPQEAVPH LEAALQEYFV ADEECRALCE
260 270 280 290 300
GPYDYDGYNY LDYSADLFQA ITDHYVQVLN CKQNCVTELA SHPSREKPFE
310 320 330 340 350
DFLPSHYNYL QFAYYNIGNY TQAIECAKTY LLFFPNDEVM HQNLAYYTAM
360 370 380 390 400
LGEEEASSIS PRENAEEYRR RSLLEKELLF FAYDIFGIPF VDPDSWTPEE
410 420 430 440 450
VIPKRLQEKQ KSERETAVRI SQEIGNLMKE IETLVEEKTK ESLDVSRLTR
460 470 480 490 500
EGGPLLYEGI SLTMNSKVLN GSQRVVMDGV ISDDECQELQ RLTNAAATSG
510 520 530 540 550
DGYRGQTSPH TPNEKFYGVT VLKALKLGQE GKVPLQSARM YYNVTEKVRR
560 570 580 590 600
VMESYFRLDT PLYFSYSHLV CRTAIEESQA ERKDSSHPVH VDNCILNAEA
610 620 630 640 650
LMCIKEPPAY TFRDYSAILY LNGDFDGGNF YFTELDAKTV TAEVQPQCGR
660 670 680 690 700
AVGFSSGTEN PHGVKAVTRG QRCAIALWFT LDPRHSERDR VQADDLVKML
710 720 730
FSPEEVDLPQ EQPLPDQQGS PEPGEESLSD RGSLHKDEL
Length:739
Mass (Da):83,651
Last modified:June 27, 2006 - v2
Checksum:i3484AE68E80B68E8
GO
Isoform 2 (identifier: Q3V1T4-2) [UniParc]FASTAAdd to basket

Also known as: GROS1-S

The sequence of this isoform differs from the canonical sequence as follows:
     540-543: MYYN → TALQ
     544-739: Missing.

Show »
Length:543
Mass (Da):61,397
Checksum:i2166EB32EC38A766
GO
Isoform 3 (identifier: Q3V1T4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):64,102
Checksum:i673B7643F80A1388
GO

Sequence cautioni

The sequence BAB27041.1 differs from that shown. Reason: Frameshift at position 707. Curated
The sequence BAE21065.1 differs from that shown.Intron retention.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 2522SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04806 (PubMed:10951563).CuratedAdd
BLAST
Sequence conflicti4 – 2522SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04807 (PubMed:10951563).CuratedAdd
BLAST
Sequence conflicti50 – 501G → R in AAF04806 (PubMed:10951563).Curated
Sequence conflicti50 – 501G → R in AAF04807 (PubMed:10951563).Curated
Sequence conflicti371 – 3722RS → PN in AAF04806 (PubMed:10951563).Curated
Sequence conflicti371 – 3722RS → PN in AAF04807 (PubMed:10951563).Curated
Sequence conflicti403 – 4031P → T in BAE21065 (PubMed:16141072).Curated
Sequence conflicti420 – 4201Missing in BAC26962 (PubMed:16141072).Curated
Sequence conflicti484 – 4841D → N in BAE35138 (PubMed:16141072).Curated
Sequence conflicti569 – 5691L → F in AAF04806 (PubMed:10951563).Curated
Sequence conflicti589 – 5891V → D in BAE21065 (PubMed:16141072).Curated
Sequence conflicti601 – 6011L → F in AAF04806 (PubMed:10951563).Curated
Sequence conflicti614 – 6141D → E in AAF04806 (PubMed:10951563).Curated
Sequence conflicti685 – 6851H → Q in BAC26962 (PubMed:16141072).Curated
Sequence conflicti716 – 7161D → G in BAE35138 (PubMed:16141072).Curated
Sequence conflicti716 – 7161D → G in AAH24047 (PubMed:19468303).Curated
Sequence conflicti727 – 73913SLSDR…HKDEL → FLHGATVLGVGIA in AAF04806 (PubMed:10951563).CuratedAdd
BLAST

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 179179Missing in isoform 3. 1 PublicationVSP_019349Add
BLAST
Alternative sequencei540 – 5434MYYN → TALQ in isoform 2. 1 PublicationVSP_019350
Alternative sequencei544 – 739196Missing in isoform 2. 1 PublicationVSP_019351Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165163 mRNA. Translation: AAF04806.1. Sequence problems.
AF165164 mRNA. Translation: AAF04807.1. Sequence problems.
AK010578 mRNA. Translation: BAB27041.1. Frameshift.
AK030436 mRNA. Translation: BAC26962.1.
AK132262 mRNA. Translation: BAE21065.1. Sequence problems.
AK159505 mRNA. Translation: BAE35138.1.
AL606975 Genomic DNA. Translation: CAM21645.1.
AL606975 Genomic DNA. Translation: CAO78098.1.
BC024047 mRNA. Translation: AAH24047.1.
CCDSiCCDS38859.1. [Q3V1T4-1]
CCDS38860.1. [Q3V1T4-3]
RefSeqiNP_001035874.1. NM_001042411.1. [Q3V1T4-3]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1]
UniGeneiMm.27961.

Genome annotation databases

EnsembliENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641. [Q3V1T4-3]
ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641. [Q3V1T4-1]
GeneIDi56401.
KEGGimmu:56401.
UCSCiuc008ulq.1. mouse. [Q3V1T4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165163 mRNA. Translation: AAF04806.1. Sequence problems.
AF165164 mRNA. Translation: AAF04807.1. Sequence problems.
AK010578 mRNA. Translation: BAB27041.1. Frameshift.
AK030436 mRNA. Translation: BAC26962.1.
AK132262 mRNA. Translation: BAE21065.1. Sequence problems.
AK159505 mRNA. Translation: BAE35138.1.
AL606975 Genomic DNA. Translation: CAM21645.1.
AL606975 Genomic DNA. Translation: CAO78098.1.
BC024047 mRNA. Translation: AAH24047.1.
CCDSiCCDS38859.1. [Q3V1T4-1]
CCDS38860.1. [Q3V1T4-3]
RefSeqiNP_001035874.1. NM_001042411.1. [Q3V1T4-3]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1]
UniGeneiMm.27961.

3D structure databases

ProteinModelPortaliQ3V1T4.
SMRiQ3V1T4. Positions 152-188, 210-236.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3V1T4. 1 interaction.
MINTiMINT-4125499.
STRINGi10090.ENSMUSP00000099723.

PTM databases

PhosphoSiteiQ3V1T4.

Proteomic databases

MaxQBiQ3V1T4.
PaxDbiQ3V1T4.
PRIDEiQ3V1T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641. [Q3V1T4-3]
ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641. [Q3V1T4-1]
GeneIDi56401.
KEGGimmu:56401.
UCSCiuc008ulq.1. mouse. [Q3V1T4-1]

Organism-specific databases

CTDi64175.
MGIiMGI:1888921. Lepre1.

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOVERGENiHBG053224.
InParanoidiQ3V1T4.
KOiK08134.
OrthoDBiEOG7BZVSS.

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.
ReactomeiREACT_285754. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiLepre1. mouse.
NextBioi312512.
PROiQ3V1T4.
SOURCEiSearch...

Gene expression databases

BgeeiQ3V1T4.
CleanExiMM_LEPRE1.
ExpressionAtlasiQ3V1T4. baseline and differential.
GenevisibleiQ3V1T4. MM.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Gros1, a potential growth suppressor on chromosome 1: its identity to basement membrane-associated proteoglycan, leprecan."
    Kaul S.C., Sugihara T., Yoshida A., Nomura H., Wadhwa R.
    Oncogene 19:3576-3583(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1/ICR.
    Tissue: Fibroblast and Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell, Pituitary and Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiP3H1_MOUSE
AccessioniPrimary (citable) accession number: Q3V1T4
Secondary accession number(s): A2A7Q4
, A6PW85, Q3TWX8, Q8BSV2, Q8CFL3, Q9CWK5, Q9QZT6, Q9QZT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: July 22, 2015
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.