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Protein

Prolyl 3-hydroxylase 1

Gene

P3h1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts (By similarity).By similarity

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi590Iron1
Metal bindingi592Iron1
Metal bindingi662Iron1
Active sitei672By similarity1

GO - Molecular functioni

GO - Biological processi

  • bone development Source: MGI
  • cell growth Source: MGI
  • collagen fibril organization Source: MGI
  • collagen metabolic process Source: MGI
  • negative regulation of post-translational protein modification Source: MGI
  • protein folding Source: MGI
  • protein hydroxylation Source: MGI
  • protein stabilization Source: MGI
  • regulation of ossification Source: MGI
  • regulation of protein secretion Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.
ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 1Imported (EC:1.14.11.7)
Alternative name(s):
Growth suppressor 11 Publication
Leucine- and proline-enriched proteoglycan 1By similarity
Short name:
Leprecan-1By similarity
Gene namesi
Name:P3h1Imported
Synonyms:Gros11 Publication, Lepre1By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1888921. P3h1.

Subcellular locationi

GO - Cellular componenti

  • basement membrane Source: MGI
  • cytoplasm Source: MGI
  • endoplasmic reticulum Source: MGI
  • extracellular exosome Source: MGI
  • membrane Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000024035326 – 739Prolyl 3-hydroxylase 1Add BLAST714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi319N-linked (GlcNAc...)Sequence analysis1
Glycosylationi470N-linked (GlcNAc...)Sequence analysis1
Glycosylationi543N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

O-glycosylated; chondroitin sulfate.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3V1T4.
PeptideAtlasiQ3V1T4.
PRIDEiQ3V1T4.

PTM databases

PhosphoSitePlusiQ3V1T4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028641.
CleanExiMM_LEPRE1.
ExpressionAtlasiQ3V1T4. baseline and differential.
GenevisibleiQ3V1T4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ3V1T4. 1 interactor.
MINTiMINT-4125499.
STRINGi10090.ENSMUSP00000099723.

Structurei

3D structure databases

ProteinModelPortaliQ3V1T4.
SMRiQ3V1T4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati36 – 69TPR 1Add BLAST34
Repeati146 – 179TPR 2Add BLAST34
Repeati208 – 241TPR 3Add BLAST34
Repeati304 – 337TPR 4Add BLAST34
Domaini567 – 681Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST115

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili404 – 442Sequence analysisAdd BLAST39

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the leprecan family.Curated
Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation
Contains 4 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
GeneTreeiENSGT00550000074573.
HOVERGENiHBG053224.
InParanoidiQ3V1T4.
KOiK08134.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3V1T4-1) [UniParc]FASTAAdd to basket
Also known as: GROS1-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVSERRLLA AMLAVAAAAA LRVAAESEPG WDVAAPDLLY AEGTAAYSRG
60 70 80 90 100
DWPGVVLNME RALRSRAALR ALRLRCRTRC ATELPWAPDL DLGPDPSLSQ
110 120 130 140 150
DPGAAALHDL RFFGAVLRRA ACLRRCLGPP SAHLLSEELD LEFNKRSPYN
160 170 180 190 200
YLQVAYFKIN KLEKAVAAAH TFFVGNPEHM EMRQNLDYYQ TMSGVKEADF
210 220 230 240 250
RDLEAKPHMH EFRLGVRLYS EEKPQEAVPH LEAALQEYFV ADEECRALCE
260 270 280 290 300
GPYDYDGYNY LDYSADLFQA ITDHYVQVLN CKQNCVTELA SHPSREKPFE
310 320 330 340 350
DFLPSHYNYL QFAYYNIGNY TQAIECAKTY LLFFPNDEVM HQNLAYYTAM
360 370 380 390 400
LGEEEASSIS PRENAEEYRR RSLLEKELLF FAYDIFGIPF VDPDSWTPEE
410 420 430 440 450
VIPKRLQEKQ KSERETAVRI SQEIGNLMKE IETLVEEKTK ESLDVSRLTR
460 470 480 490 500
EGGPLLYEGI SLTMNSKVLN GSQRVVMDGV ISDDECQELQ RLTNAAATSG
510 520 530 540 550
DGYRGQTSPH TPNEKFYGVT VLKALKLGQE GKVPLQSARM YYNVTEKVRR
560 570 580 590 600
VMESYFRLDT PLYFSYSHLV CRTAIEESQA ERKDSSHPVH VDNCILNAEA
610 620 630 640 650
LMCIKEPPAY TFRDYSAILY LNGDFDGGNF YFTELDAKTV TAEVQPQCGR
660 670 680 690 700
AVGFSSGTEN PHGVKAVTRG QRCAIALWFT LDPRHSERDR VQADDLVKML
710 720 730
FSPEEVDLPQ EQPLPDQQGS PEPGEESLSD RGSLHKDEL
Length:739
Mass (Da):83,651
Last modified:June 27, 2006 - v2
Checksum:i3484AE68E80B68E8
GO
Isoform 2 (identifier: Q3V1T4-2) [UniParc]FASTAAdd to basket
Also known as: GROS1-S

The sequence of this isoform differs from the canonical sequence as follows:
     540-543: MYYN → TALQ
     544-739: Missing.

Show »
Length:543
Mass (Da):61,397
Checksum:i2166EB32EC38A766
GO
Isoform 3 (identifier: Q3V1T4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.

Note: No experimental confirmation available.
Show »
Length:560
Mass (Da):64,102
Checksum:i673B7643F80A1388
GO

Sequence cautioni

The sequence BAB27041 differs from that shown. Reason: Frameshift at position 707.Curated
The sequence BAE21065 differs from that shown. Intron retention.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4 – 25SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04806 (PubMed:10951563).CuratedAdd BLAST22
Sequence conflicti4 – 25SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04807 (PubMed:10951563).CuratedAdd BLAST22
Sequence conflicti50G → R in AAF04806 (PubMed:10951563).Curated1
Sequence conflicti50G → R in AAF04807 (PubMed:10951563).Curated1
Sequence conflicti371 – 372RS → PN in AAF04806 (PubMed:10951563).Curated2
Sequence conflicti371 – 372RS → PN in AAF04807 (PubMed:10951563).Curated2
Sequence conflicti403P → T in BAE21065 (PubMed:16141072).Curated1
Sequence conflicti420Missing in BAC26962 (PubMed:16141072).Curated1
Sequence conflicti484D → N in BAE35138 (PubMed:16141072).Curated1
Sequence conflicti569L → F in AAF04806 (PubMed:10951563).Curated1
Sequence conflicti589V → D in BAE21065 (PubMed:16141072).Curated1
Sequence conflicti601L → F in AAF04806 (PubMed:10951563).Curated1
Sequence conflicti614D → E in AAF04806 (PubMed:10951563).Curated1
Sequence conflicti685H → Q in BAC26962 (PubMed:16141072).Curated1
Sequence conflicti716D → G in BAE35138 (PubMed:16141072).Curated1
Sequence conflicti716D → G in AAH24047 (PubMed:19468303).Curated1
Sequence conflicti727 – 739SLSDR…HKDEL → FLHGATVLGVGIA in AAF04806 (PubMed:10951563).CuratedAdd BLAST13

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0193491 – 179Missing in isoform 3. 1 PublicationAdd BLAST179
Alternative sequenceiVSP_019350540 – 543MYYN → TALQ in isoform 2. 1 Publication4
Alternative sequenceiVSP_019351544 – 739Missing in isoform 2. 1 PublicationAdd BLAST196

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165163 mRNA. Translation: AAF04806.1. Sequence problems.
AF165164 mRNA. Translation: AAF04807.1. Sequence problems.
AK010578 mRNA. Translation: BAB27041.1. Frameshift.
AK030436 mRNA. Translation: BAC26962.1.
AK132262 mRNA. Translation: BAE21065.1. Sequence problems.
AK159505 mRNA. Translation: BAE35138.1.
AL606975 Genomic DNA. Translation: CAM21645.1.
AL606975 Genomic DNA. Translation: CAO78098.1.
BC024047 mRNA. Translation: AAH24047.1.
CCDSiCCDS38859.1. [Q3V1T4-1]
CCDS38860.1. [Q3V1T4-3]
RefSeqiNP_001035874.1. NM_001042411.1. [Q3V1T4-3]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1]
UniGeneiMm.27961.

Genome annotation databases

EnsembliENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641. [Q3V1T4-3]
ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641. [Q3V1T4-1]
GeneIDi56401.
KEGGimmu:56401.
UCSCiuc008ulq.1. mouse. [Q3V1T4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165163 mRNA. Translation: AAF04806.1. Sequence problems.
AF165164 mRNA. Translation: AAF04807.1. Sequence problems.
AK010578 mRNA. Translation: BAB27041.1. Frameshift.
AK030436 mRNA. Translation: BAC26962.1.
AK132262 mRNA. Translation: BAE21065.1. Sequence problems.
AK159505 mRNA. Translation: BAE35138.1.
AL606975 Genomic DNA. Translation: CAM21645.1.
AL606975 Genomic DNA. Translation: CAO78098.1.
BC024047 mRNA. Translation: AAH24047.1.
CCDSiCCDS38859.1. [Q3V1T4-1]
CCDS38860.1. [Q3V1T4-3]
RefSeqiNP_001035874.1. NM_001042411.1. [Q3V1T4-3]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1]
UniGeneiMm.27961.

3D structure databases

ProteinModelPortaliQ3V1T4.
SMRiQ3V1T4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ3V1T4. 1 interactor.
MINTiMINT-4125499.
STRINGi10090.ENSMUSP00000099723.

PTM databases

PhosphoSitePlusiQ3V1T4.

Proteomic databases

PaxDbiQ3V1T4.
PeptideAtlasiQ3V1T4.
PRIDEiQ3V1T4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641. [Q3V1T4-3]
ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641. [Q3V1T4-1]
GeneIDi56401.
KEGGimmu:56401.
UCSCiuc008ulq.1. mouse. [Q3V1T4-1]

Organism-specific databases

CTDi64175.
MGIiMGI:1888921. P3h1.

Phylogenomic databases

eggNOGiKOG4459. Eukaryota.
ENOG410XPXF. LUCA.
GeneTreeiENSGT00550000074573.
HOVERGENiHBG053224.
InParanoidiQ3V1T4.
KOiK08134.

Enzyme and pathway databases

BRENDAi1.14.11.7. 3474.
ReactomeiR-MMU-1650814. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

ChiTaRSiLepre1. mouse.
PROiQ3V1T4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028641.
CleanExiMM_LEPRE1.
ExpressionAtlasiQ3V1T4. baseline and differential.
GenevisibleiQ3V1T4. MM.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP3H1_MOUSE
AccessioniPrimary (citable) accession number: Q3V1T4
Secondary accession number(s): A2A7Q4
, A6PW85, Q3TWX8, Q8BSV2, Q8CFL3, Q9CWK5, Q9QZT6, Q9QZT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.