SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q3V1T4

- P3H1_MOUSE

UniProt

Q3V1T4 - P3H1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Prolyl 3-hydroxylase 1

Gene
Lepre1, Gros1, P3h1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Basement membrane-associated chondroitin sulfate proteoglycan (CSPG). Has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens, especially types IV and V. May be involved in the secretory pathway of cells. Has growth suppressive activity in fibroblasts By similarity.

Catalytic activityi

L-proline-[procollagen] + 2-oxoglutarate + O2 = trans-3-hydroxy-L-proline-[procollagen] + succinate + CO2.

Cofactori

Iron By similarity.
Ascorbate By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi590 – 5901Iron
Metal bindingi592 – 5921Iron
Metal bindingi662 – 6621Iron
Active sitei672 – 6721 By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. L-ascorbic acid binding Source: UniProtKB-KW
  3. procollagen-proline 3-dioxygenase activity Source: MGI

GO - Biological processi

  1. cell growth Source: MGI
  2. collagen fibril organization Source: MGI
  3. collagen metabolic process Source: MGI
  4. peptidyl-proline hydroxylation Source: GOC
  5. regulation of ossification Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

ReactomeiREACT_198984. Collagen biosynthesis and modifying enzymes.

Names & Taxonomyi

Protein namesi
Recommended name:
Prolyl 3-hydroxylase 1 (EC:1.14.11.7)
Alternative name(s):
Growth suppressor 1
Leucine- and proline-enriched proteoglycan 1
Short name:
Leprecan-1
Gene namesi
Name:Lepre1
Synonyms:Gros1, P3h1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1888921. Lepre1.

Subcellular locationi

Endoplasmic reticulum By similarity. Secretedextracellular spaceextracellular matrix By similarity
Note: Secreted into the extracellular matrix as a chondroitin sulfate proteoglycan (CSPG).

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. endoplasmic reticulum Source: MGI
  3. nucleus Source: MGI
  4. plasma membrane Source: MGI
  5. proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 Reviewed predictionAdd
BLAST
Chaini26 – 739714Prolyl 3-hydroxylase 1PRO_0000240353Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi319 – 3191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi470 – 4701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi543 – 5431N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

O-glycosylated; chondroitin sulfate By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3V1T4.
PRIDEiQ3V1T4.

PTM databases

PhosphoSiteiQ3V1T4.

Expressioni

Gene expression databases

ArrayExpressiQ3V1T4.
BgeeiQ3V1T4.
CleanExiMM_LEPRE1.
GenevestigatoriQ3V1T4.

Interactioni

Protein-protein interaction databases

IntActiQ3V1T4. 1 interaction.
MINTiMINT-4125499.

Structurei

3D structure databases

ProteinModelPortaliQ3V1T4.
SMRiQ3V1T4. Positions 34-65.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati36 – 6934TPR 1Add
BLAST
Repeati146 – 17934TPR 2Add
BLAST
Repeati208 – 24134TPR 3Add
BLAST
Repeati304 – 33734TPR 4Add
BLAST
Domaini567 – 681115Fe2OG dioxygenaseAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili404 – 44239 Reviewed predictionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 7394Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Belongs to the leprecan family.
Contains 4 TPR repeats.

Keywords - Domaini

Coiled coil, Repeat, Signal, TPR repeat

Phylogenomic databases

eggNOGiNOG269251.
GeneTreeiENSGT00550000074573.
HOVERGENiHBG053224.
KOiK08134.
OrthoDBiEOG7BZVSS.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
[Graphical view]
PfamiPF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view]
SMARTiSM00702. P4Hc. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3V1T4-1) [UniParc]FASTAAdd to Basket

Also known as: GROS1-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAVSERRLLA AMLAVAAAAA LRVAAESEPG WDVAAPDLLY AEGTAAYSRG    50
DWPGVVLNME RALRSRAALR ALRLRCRTRC ATELPWAPDL DLGPDPSLSQ 100
DPGAAALHDL RFFGAVLRRA ACLRRCLGPP SAHLLSEELD LEFNKRSPYN 150
YLQVAYFKIN KLEKAVAAAH TFFVGNPEHM EMRQNLDYYQ TMSGVKEADF 200
RDLEAKPHMH EFRLGVRLYS EEKPQEAVPH LEAALQEYFV ADEECRALCE 250
GPYDYDGYNY LDYSADLFQA ITDHYVQVLN CKQNCVTELA SHPSREKPFE 300
DFLPSHYNYL QFAYYNIGNY TQAIECAKTY LLFFPNDEVM HQNLAYYTAM 350
LGEEEASSIS PRENAEEYRR RSLLEKELLF FAYDIFGIPF VDPDSWTPEE 400
VIPKRLQEKQ KSERETAVRI SQEIGNLMKE IETLVEEKTK ESLDVSRLTR 450
EGGPLLYEGI SLTMNSKVLN GSQRVVMDGV ISDDECQELQ RLTNAAATSG 500
DGYRGQTSPH TPNEKFYGVT VLKALKLGQE GKVPLQSARM YYNVTEKVRR 550
VMESYFRLDT PLYFSYSHLV CRTAIEESQA ERKDSSHPVH VDNCILNAEA 600
LMCIKEPPAY TFRDYSAILY LNGDFDGGNF YFTELDAKTV TAEVQPQCGR 650
AVGFSSGTEN PHGVKAVTRG QRCAIALWFT LDPRHSERDR VQADDLVKML 700
FSPEEVDLPQ EQPLPDQQGS PEPGEESLSD RGSLHKDEL 739
Length:739
Mass (Da):83,651
Last modified:June 27, 2006 - v2
Checksum:i3484AE68E80B68E8
GO
Isoform 2 (identifier: Q3V1T4-2) [UniParc]FASTAAdd to Basket

Also known as: GROS1-S

The sequence of this isoform differs from the canonical sequence as follows:
     540-543: MYYN → TALQ
     544-739: Missing.

Show »
Length:543
Mass (Da):61,397
Checksum:i2166EB32EC38A766
GO
Isoform 3 (identifier: Q3V1T4-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-179: Missing.

Note: No experimental confirmation available.

Show »
Length:560
Mass (Da):64,102
Checksum:i673B7643F80A1388
GO

Sequence cautioni

The sequence BAE21065.1 differs from that shown. Reason: Intron retention.
The sequence BAB27041.1 differs from that shown. Reason: Frameshift at position 707.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 179179Missing in isoform 3. VSP_019349Add
BLAST
Alternative sequencei540 – 5434MYYN → TALQ in isoform 2. VSP_019350
Alternative sequencei544 – 739196Missing in isoform 2. VSP_019351Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 2522SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04806. 1 PublicationAdd
BLAST
Sequence conflicti4 – 2522SERRL…LRVAA → TKGGCWHDASGRRRRRLTGC G in AAF04807. 1 PublicationAdd
BLAST
Sequence conflicti50 – 501G → R in AAF04806. 1 Publication
Sequence conflicti50 – 501G → R in AAF04807. 1 Publication
Sequence conflicti371 – 3722RS → PN in AAF04806. 1 Publication
Sequence conflicti371 – 3722RS → PN in AAF04807. 1 Publication
Sequence conflicti403 – 4031P → T in BAE21065. 1 Publication
Sequence conflicti420 – 4201Missing in BAC26962. 1 Publication
Sequence conflicti484 – 4841D → N in BAE35138. 1 Publication
Sequence conflicti569 – 5691L → F in AAF04806. 1 Publication
Sequence conflicti589 – 5891V → D in BAE21065. 1 Publication
Sequence conflicti601 – 6011L → F in AAF04806. 1 Publication
Sequence conflicti614 – 6141D → E in AAF04806. 1 Publication
Sequence conflicti685 – 6851H → Q in BAC26962. 1 Publication
Sequence conflicti716 – 7161D → G in BAE35138. 1 Publication
Sequence conflicti716 – 7161D → G in AAH24047. 1 Publication
Sequence conflicti727 – 73913SLSDR…HKDEL → FLHGATVLGVGIA in AAF04806. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF165163 mRNA. Translation: AAF04806.1. Sequence problems.
AF165164 mRNA. Translation: AAF04807.1. Sequence problems.
AK010578 mRNA. Translation: BAB27041.1. Frameshift.
AK030436 mRNA. Translation: BAC26962.1.
AK132262 mRNA. Translation: BAE21065.1. Sequence problems.
AK159505 mRNA. Translation: BAE35138.1.
AL606975 Genomic DNA. Translation: CAM21645.1.
AL606975 Genomic DNA. Translation: CAO78098.1.
BC024047 mRNA. Translation: AAH24047.1.
CCDSiCCDS38859.1. [Q3V1T4-1]
CCDS38860.1. [Q3V1T4-3]
RefSeqiNP_001035874.1. NM_001042411.1. [Q3V1T4-3]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1]
UniGeneiMm.27961.

Genome annotation databases

EnsembliENSMUST00000081606; ENSMUSP00000080312; ENSMUSG00000028641. [Q3V1T4-3]
ENSMUST00000121111; ENSMUSP00000112504; ENSMUSG00000028641. [Q3V1T4-1]
GeneIDi56401.
KEGGimmu:56401.
UCSCiuc008ulq.1. mouse. [Q3V1T4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF165163 mRNA. Translation: AAF04806.1 . Sequence problems.
AF165164 mRNA. Translation: AAF04807.1 . Sequence problems.
AK010578 mRNA. Translation: BAB27041.1 . Frameshift.
AK030436 mRNA. Translation: BAC26962.1 .
AK132262 mRNA. Translation: BAE21065.1 . Sequence problems.
AK159505 mRNA. Translation: BAE35138.1 .
AL606975 Genomic DNA. Translation: CAM21645.1 .
AL606975 Genomic DNA. Translation: CAO78098.1 .
BC024047 mRNA. Translation: AAH24047.1 .
CCDSi CCDS38859.1. [Q3V1T4-1 ]
CCDS38860.1. [Q3V1T4-3 ]
RefSeqi NP_001035874.1. NM_001042411.1. [Q3V1T4-3 ]
NP_001273077.1. NM_001286148.1.
NP_062756.2. NM_019782.3.
NP_062757.2. NM_019783.2. [Q3V1T4-1 ]
UniGenei Mm.27961.

3D structure databases

ProteinModelPortali Q3V1T4.
SMRi Q3V1T4. Positions 34-65.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q3V1T4. 1 interaction.
MINTi MINT-4125499.

PTM databases

PhosphoSitei Q3V1T4.

Proteomic databases

PaxDbi Q3V1T4.
PRIDEi Q3V1T4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000081606 ; ENSMUSP00000080312 ; ENSMUSG00000028641 . [Q3V1T4-3 ]
ENSMUST00000121111 ; ENSMUSP00000112504 ; ENSMUSG00000028641 . [Q3V1T4-1 ]
GeneIDi 56401.
KEGGi mmu:56401.
UCSCi uc008ulq.1. mouse. [Q3V1T4-1 ]

Organism-specific databases

CTDi 64175.
MGIi MGI:1888921. Lepre1.

Phylogenomic databases

eggNOGi NOG269251.
GeneTreei ENSGT00550000074573.
HOVERGENi HBG053224.
KOi K08134.
OrthoDBi EOG7BZVSS.

Enzyme and pathway databases

Reactomei REACT_198984. Collagen biosynthesis and modifying enzymes.

Miscellaneous databases

NextBioi 312512.
PROi Q3V1T4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q3V1T4.
Bgeei Q3V1T4.
CleanExi MM_LEPRE1.
Genevestigatori Q3V1T4.

Family and domain databases

Gene3Di 1.25.40.10. 3 hits.
InterProi IPR005123. Oxoglu/Fe-dep_dioxygenase.
IPR006620. Pro_4_hyd_alph.
IPR011990. TPR-like_helical.
[Graphical view ]
Pfami PF13640. 2OG-FeII_Oxy_3. 1 hit.
[Graphical view ]
SMARTi SM00702. P4Hc. 1 hit.
[Graphical view ]
PROSITEi PS00014. ER_TARGET. 1 hit.
PS51471. FE2OG_OXY. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gros1, a potential growth suppressor on chromosome 1: its identity to basement membrane-associated proteoglycan, leprecan."
    Kaul S.C., Sugihara T., Yoshida A., Nomura H., Wadhwa R.
    Oncogene 19:3576-3583(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1/ICR.
    Tissue: Fibroblast and Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell, Pituitary and Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiP3H1_MOUSE
AccessioniPrimary (citable) accession number: Q3V1T4
Secondary accession number(s): A2A7Q4
, A6PW85, Q3TWX8, Q8BSV2, Q8CFL3, Q9CWK5, Q9QZT6, Q9QZT7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: June 27, 2006
Last modified: September 3, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi