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Protein

Cytosolic purine 5'-nucleotidase

Gene

Nt5c2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides (By similarity).By similarity

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Allosterically activated by various compounds, including ATP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei52NucleophileBy similarity1
Metal bindingi52MagnesiumBy similarity1
Active sitei54Proton donorBy similarity1
Metal bindingi54Magnesium; via carbonyl oxygenBy similarity1
Binding sitei127Allosteric activator 1By similarity1
Binding sitei154Allosteric activator 2By similarity1
Metal bindingi351MagnesiumBy similarity1
Binding sitei354Allosteric activator 2By similarity1
Binding sitei436Allosteric activator 1; via carbonyl oxygenBy similarity1
Binding sitei453Allosteric activator 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2161541. Abacavir metabolism.
R-MMU-74259. Purine catabolism.
SABIO-RKQ3V1L4.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic purine 5'-nucleotidase (EC:3.1.3.5)
Alternative name(s):
Cytosolic 5'-nucleotidase II
Gene namesi
Name:Nt5c2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2178563. Nt5c2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003102641 – 560Cytosolic purine 5'-nucleotidaseAdd BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei418PhosphoserineBy similarity1
Modified residuei502PhosphoserineBy similarity1
Modified residuei511PhosphoserineBy similarity1
Modified residuei527PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3V1L4.
MaxQBiQ3V1L4.
PaxDbiQ3V1L4.
PRIDEiQ3V1L4.

PTM databases

iPTMnetiQ3V1L4.
PhosphoSitePlusiQ3V1L4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025041.
CleanExiMM_NT5C2.
ExpressionAtlasiQ3V1L4. baseline and differential.
GenevisibleiQ3V1L4. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi218423. 1 interactor.
IntActiQ3V1L4. 2 interactors.
MINTiMINT-4113584.
STRINGi10090.ENSMUSP00000130898.

Structurei

3D structure databases

ProteinModelPortaliQ3V1L4.
SMRiQ3V1L4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni202 – 210Substrate bindingSequence analysis9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi549 – 560Asp/Glu-rich (acidic)Add BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2469. Eukaryota.
ENOG410XQAV. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000246075.
HOVERGENiHBG000025.
InParanoidiQ3V1L4.
KOiK01081.
PhylomeDBiQ3V1L4.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3V1L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSWSDRLQ NAADVPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG
60 70 80 90 100
FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL
110 120 130 140 150
VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF
160 170 180 190 200
YILNTLFNLP ETYLLACLVD FFTNCPRYTS CDTGFKDGDL FMSYRSMFQD
210 220 230 240 250
VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN
260 270 280 290 300
SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT
310 320 330 340 350
VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG
360 370 380 390 400
DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA
410 420 430 440 450
ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF
460 470 480 490 500
ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM
510 520 530 540 550
ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE
560
DDDEEEEEEE
Length:560
Mass (Da):64,809
Last modified:November 13, 2007 - v2
Checksum:iBC1EBB45E6E1F357
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti89F → Y in BAE21136 (PubMed:16141072).Curated1
Sequence conflicti217K → R in BAC29555 (PubMed:16141072).Curated1
Sequence conflicti531I → V in AAH64760 (PubMed:15489334).Curated1
Sequence conflicti535N → D in AAH64760 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008045 mRNA. Translation: BAB25428.1.
AK036730 mRNA. Translation: BAC29555.1.
AK132384 mRNA. Translation: BAE21136.1.
AK147901 mRNA. Translation: BAE28216.1.
AK169616 mRNA. Translation: BAE41263.1.
BC064760 mRNA. Translation: AAH64760.1.
CCDSiCCDS29883.1.
RefSeqiNP_001157835.1. NM_001164363.1.
NP_001157837.1. NM_001164365.1.
NP_084086.3. NM_029810.4.
XP_006527521.1. XM_006527458.2.
XP_006527522.1. XM_006527459.2.
UniGeneiMm.40965.

Genome annotation databases

EnsembliENSMUST00000168536; ENSMUSP00000129126; ENSMUSG00000025041.
GeneIDi76952.
KEGGimmu:76952.
UCSCiuc008hug.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008045 mRNA. Translation: BAB25428.1.
AK036730 mRNA. Translation: BAC29555.1.
AK132384 mRNA. Translation: BAE21136.1.
AK147901 mRNA. Translation: BAE28216.1.
AK169616 mRNA. Translation: BAE41263.1.
BC064760 mRNA. Translation: AAH64760.1.
CCDSiCCDS29883.1.
RefSeqiNP_001157835.1. NM_001164363.1.
NP_001157837.1. NM_001164365.1.
NP_084086.3. NM_029810.4.
XP_006527521.1. XM_006527458.2.
XP_006527522.1. XM_006527459.2.
UniGeneiMm.40965.

3D structure databases

ProteinModelPortaliQ3V1L4.
SMRiQ3V1L4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218423. 1 interactor.
IntActiQ3V1L4. 2 interactors.
MINTiMINT-4113584.
STRINGi10090.ENSMUSP00000130898.

PTM databases

iPTMnetiQ3V1L4.
PhosphoSitePlusiQ3V1L4.

Proteomic databases

EPDiQ3V1L4.
MaxQBiQ3V1L4.
PaxDbiQ3V1L4.
PRIDEiQ3V1L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000168536; ENSMUSP00000129126; ENSMUSG00000025041.
GeneIDi76952.
KEGGimmu:76952.
UCSCiuc008hug.2. mouse.

Organism-specific databases

CTDi22978.
MGIiMGI:2178563. Nt5c2.

Phylogenomic databases

eggNOGiKOG2469. Eukaryota.
ENOG410XQAV. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000246075.
HOVERGENiHBG000025.
InParanoidiQ3V1L4.
KOiK01081.
PhylomeDBiQ3V1L4.

Enzyme and pathway databases

ReactomeiR-MMU-2161541. Abacavir metabolism.
R-MMU-74259. Purine catabolism.
SABIO-RKQ3V1L4.

Miscellaneous databases

ChiTaRSiNt5c2. mouse.
PROiQ3V1L4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025041.
CleanExiMM_NT5C2.
ExpressionAtlasiQ3V1L4. baseline and differential.
GenevisibleiQ3V1L4. MM.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei5NTC_MOUSE
AccessioniPrimary (citable) accession number: Q3V1L4
Secondary accession number(s): Q6P223, Q8BZ43, Q9D8G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: November 2, 2016
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.