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Protein

Cytosolic purine 5'-nucleotidase

Gene

Nt5c2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases. Preferentially hydrolyzes inosine 5'-monophosphate (IMP) and other purine nucleotides (By similarity).By similarity

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Allosterically activated by various compounds, including ATP.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521NucleophileBy similarity
Metal bindingi52 – 521MagnesiumBy similarity
Active sitei54 – 541Proton donorBy similarity
Metal bindingi54 – 541Magnesium; via carbonyl oxygenBy similarity
Binding sitei127 – 1271Allosteric activator 1By similarity
Binding sitei154 – 1541Allosteric activator 2By similarity
Metal bindingi351 – 3511MagnesiumBy similarity
Binding sitei354 – 3541Allosteric activator 2By similarity
Binding sitei436 – 4361Allosteric activator 1; via carbonyl oxygenBy similarity
Binding sitei453 – 4531Allosteric activator 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2161541. Abacavir metabolism.
R-MMU-74259. Purine catabolism.
SABIO-RKQ3V1L4.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic purine 5'-nucleotidase (EC:3.1.3.5)
Alternative name(s):
Cytosolic 5'-nucleotidase II
Gene namesi
Name:Nt5c2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2178563. Nt5c2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Cytosolic purine 5'-nucleotidasePRO_0000310264Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei418 – 4181PhosphoserineBy similarity
Modified residuei502 – 5021PhosphoserineBy similarity
Modified residuei511 – 5111PhosphoserineBy similarity
Modified residuei527 – 5271PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3V1L4.
MaxQBiQ3V1L4.
PaxDbiQ3V1L4.
PRIDEiQ3V1L4.

PTM databases

iPTMnetiQ3V1L4.
PhosphoSiteiQ3V1L4.

Expressioni

Gene expression databases

BgeeiQ3V1L4.
CleanExiMM_NT5C2.
ExpressionAtlasiQ3V1L4. baseline and differential.
GenevisibleiQ3V1L4. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi218423. 1 interaction.
IntActiQ3V1L4. 2 interactions.
MINTiMINT-4113584.
STRINGi10090.ENSMUSP00000130898.

Structurei

3D structure databases

ProteinModelPortaliQ3V1L4.
SMRiQ3V1L4. Positions 3-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni202 – 2109Substrate bindingSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi549 – 56012Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2469. Eukaryota.
ENOG410XQAV. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000246075.
HOVERGENiHBG000025.
InParanoidiQ3V1L4.
KOiK01081.
PhylomeDBiQ3V1L4.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3V1L4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSWSDRLQ NAADVPANMD KHALKKYRRE AYHRVFVNRS LAMEKIKCFG
60 70 80 90 100
FDMDYTLAVY KSPEYESLGF ELTVERLVSI GYPQELLSFA YDSTFPTRGL
110 120 130 140 150
VFDTLYGNLL KVDAYGNLLV CAHGFNFIRG PETREQYPNK FIQRDDTERF
160 170 180 190 200
YILNTLFNLP ETYLLACLVD FFTNCPRYTS CDTGFKDGDL FMSYRSMFQD
210 220 230 240 250
VRDAVDWVHY KGSLKEKTVE NLEKYVVKDG KLPLLLSRMK EVGKVFLATN
260 270 280 290 300
SDYKYTDKIM TYLFDFPHGP KPGSSHRPWQ SYFDLILVDA RKPLFFGEGT
310 320 330 340 350
VLRQVDTKTG KLKIGTYTGP LQHGIVYSGG SSDTICDLLG AKGKDILYIG
360 370 380 390 400
DHIFGDILKS KKRQGWRTFL VIPELAQELH VWTDKSSLFE ELQSLDIFLA
410 420 430 440 450
ELYKHLDSSS NERPDISSIQ RRIKKVTHDM DMCYGMMGSL FRSGSRQTLF
460 470 480 490 500
ASQVMRYADL YAASFINLLY YPFSYLFRAA HVLMPHESTV EHTHVDINEM
510 520 530 540 550
ESPLATRNRT SVDFKDTDYK RHQLTRSISE IKPPNLFPLA PQEITHCHDE
560
DDDEEEEEEE
Length:560
Mass (Da):64,809
Last modified:November 13, 2007 - v2
Checksum:iBC1EBB45E6E1F357
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti89 – 891F → Y in BAE21136 (PubMed:16141072).Curated
Sequence conflicti217 – 2171K → R in BAC29555 (PubMed:16141072).Curated
Sequence conflicti531 – 5311I → V in AAH64760 (PubMed:15489334).Curated
Sequence conflicti535 – 5351N → D in AAH64760 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008045 mRNA. Translation: BAB25428.1.
AK036730 mRNA. Translation: BAC29555.1.
AK132384 mRNA. Translation: BAE21136.1.
AK147901 mRNA. Translation: BAE28216.1.
AK169616 mRNA. Translation: BAE41263.1.
BC064760 mRNA. Translation: AAH64760.1.
CCDSiCCDS29883.1.
RefSeqiNP_001157835.1. NM_001164363.1.
NP_001157837.1. NM_001164365.1.
NP_084086.3. NM_029810.4.
XP_006527521.1. XM_006527458.2.
XP_006527522.1. XM_006527459.2.
UniGeneiMm.40965.

Genome annotation databases

EnsembliENSMUST00000168536; ENSMUSP00000129126; ENSMUSG00000025041.
GeneIDi76952.
KEGGimmu:76952.
UCSCiuc008hug.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK008045 mRNA. Translation: BAB25428.1.
AK036730 mRNA. Translation: BAC29555.1.
AK132384 mRNA. Translation: BAE21136.1.
AK147901 mRNA. Translation: BAE28216.1.
AK169616 mRNA. Translation: BAE41263.1.
BC064760 mRNA. Translation: AAH64760.1.
CCDSiCCDS29883.1.
RefSeqiNP_001157835.1. NM_001164363.1.
NP_001157837.1. NM_001164365.1.
NP_084086.3. NM_029810.4.
XP_006527521.1. XM_006527458.2.
XP_006527522.1. XM_006527459.2.
UniGeneiMm.40965.

3D structure databases

ProteinModelPortaliQ3V1L4.
SMRiQ3V1L4. Positions 3-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218423. 1 interaction.
IntActiQ3V1L4. 2 interactions.
MINTiMINT-4113584.
STRINGi10090.ENSMUSP00000130898.

PTM databases

iPTMnetiQ3V1L4.
PhosphoSiteiQ3V1L4.

Proteomic databases

EPDiQ3V1L4.
MaxQBiQ3V1L4.
PaxDbiQ3V1L4.
PRIDEiQ3V1L4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000168536; ENSMUSP00000129126; ENSMUSG00000025041.
GeneIDi76952.
KEGGimmu:76952.
UCSCiuc008hug.2. mouse.

Organism-specific databases

CTDi22978.
MGIiMGI:2178563. Nt5c2.

Phylogenomic databases

eggNOGiKOG2469. Eukaryota.
ENOG410XQAV. LUCA.
GeneTreeiENSGT00550000074539.
HOGENOMiHOG000246075.
HOVERGENiHBG000025.
InParanoidiQ3V1L4.
KOiK01081.
PhylomeDBiQ3V1L4.

Enzyme and pathway databases

ReactomeiR-MMU-2161541. Abacavir metabolism.
R-MMU-74259. Purine catabolism.
SABIO-RKQ3V1L4.

Miscellaneous databases

ChiTaRSiNt5c2. mouse.
PROiQ3V1L4.
SOURCEiSearch...

Gene expression databases

BgeeiQ3V1L4.
CleanExiMM_NT5C2.
ExpressionAtlasiQ3V1L4. baseline and differential.
GenevisibleiQ3V1L4. MM.

Family and domain databases

Gene3Di3.40.50.1000. 3 hits.
InterProiIPR023214. HAD-like_dom.
IPR008380. HAD-SF_hydro_IG_5-nucl.
IPR016695. Pur_nucleotidase.
[Graphical view]
PANTHERiPTHR12103. PTHR12103. 1 hit.
PfamiPF05761. 5_nucleotid. 1 hit.
[Graphical view]
PIRSFiPIRSF017434. Purine_5'-nucleotidase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR02244. HAD-IG-Ncltidse. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone, Head, Small intestine and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-527, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry namei5NTC_MOUSE
AccessioniPrimary (citable) accession number: Q3V1L4
Secondary accession number(s): Q6P223, Q8BZ43, Q9D8G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: November 13, 2007
Last modified: July 6, 2016
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.