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Protein

Ubiquitin carboxyl-terminal hydrolase 48

Gene

Usp48

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98NucleophilePROSITE-ProRule annotation1
Active sitei353Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.068.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 48 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 48
Ubiquitin thioesterase 48
Ubiquitin-specific-processing protease 48
Gene namesi
Name:Usp48
Synonyms:Kiaa4202
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2158502. Usp48.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002495241 – 1052Ubiquitin carboxyl-terminal hydrolase 48Add BLAST1052

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei903PhosphoserineBy similarity1
Modified residuei904PhosphoserineBy similarity1
Modified residuei905PhosphoserineBy similarity1
Modified residuei973N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ3V0C5.
PeptideAtlasiQ3V0C5.
PRIDEiQ3V0C5.

PTM databases

iPTMnetiQ3V0C5.
PhosphoSitePlusiQ3V0C5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000043411.
CleanExiMM_USP48.
ExpressionAtlasiQ3V0C5. baseline and differential.
GenevisibleiQ3V0C5. MM.

Interactioni

Subunit structurei

Interacts with TRAF2 and RELA.By similarity

Protein-protein interaction databases

BioGridi228386. 2 interactors.
STRINGi10090.ENSMUSP00000055016.

Structurei

3D structure databases

ProteinModelPortaliQ3V0C5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 420USPAdd BLAST332
Domaini459 – 553DUSP 1PROSITE-ProRule annotationAdd BLAST95
Domaini568 – 691DUSP 2PROSITE-ProRule annotationAdd BLAST124
Domaini710 – 841DUSP 3PROSITE-ProRule annotationAdd BLAST132
Domaini946 – 1026Ubiquitin-likePROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1863. Eukaryota.
COG5077. LUCA.
GeneTreeiENSGT00760000119158.
HOGENOMiHOG000231639.
HOVERGENiHBG080368.
InParanoidiQ3V0C5.
KOiK11858.
OMAiCSHGKLH.
OrthoDBiEOG091G00SZ.
PhylomeDBiQ3V0C5.
TreeFamiTF106280.

Family and domain databases

CDDicd02668. Peptidase_C19L. 1 hit.
Gene3Di3.30.2230.10. 3 hits.
InterProiView protein in InterPro
IPR035927. DUSP-like_sf.
IPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-like_domsf.
IPR000626. Ubiquitin_dom.
IPR033841. USP48.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
SUPFAMiSSF143791. SSF143791. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEiView protein in PROSITE
PS51283. DUSP. 3 hits.
PS50053. UBIQUITIN_2. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3V0C5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG
60 70 80 90 100
NPNCLVGIGE HIWLGEIDEN SFHSIDDPNC ERRKKNSFVG LTNLGATCYV
110 120 130 140 150
NTFLQVWFLN LELRQALYLC PSTCSDYTKG DGIHGGKDYE PQTICEHLQY
160 170 180 190 200
LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ DAQEFSKLFM SLLEDTLSKQ
210 220 230 240 250
KNPDVRNVVQ QQFCGEYAYV TVCNQCGRES KLVSKFYELE LNIQGHKQLT
260 270 280 290 300
DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV
310 320 330 340 350
FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY
360 370 380 390 400
SGHYIAHVKD PQSGEWYKFN DEDIEKMEGK KLQLGIEEDL EPSKSQTRKP
410 420 430 440 450
KCGKGTHCSR NAYMLVYRLQ AQEKNHTMVQ VPAFLQELVD RDNSKFEEWC
460 470 480 490 500
VEMAEMRKQS VDKGKAKHEE VKELYQRLPA GAEPYEFVSL EWLQKWLDES
510 520 530 540 550
TPTKPIDNNA CLCSHDKLHP DKISIMKRIS EYAANIFYSR YGGGPRLTVK
560 570 580 590 600
ALCKDCVVER CRVLRLKNQL NEDYKAVNNL LKSTMKGDGF WVGKSSLRSW
610 620 630 640 650
RQLALEQLDE QDGEAEQSNG KINGSTFNKD ESKEEKKEEE EELNFNEDIL
660 670 680 690 700
CPHGELSISE NERRLVSQEA WSKLQQYFPK APEFPSYREC CSQCKILERE
710 720 730 740 750
GEENEALHKM IANEQKTSLP NLFQDKNRPC LSNWPEDTDA LYIVSHFFLD
760 770 780 790 800
EWRKFVRKPA RSAPVSSIGN AALLCPHGGL MFTFPSLTKE DSKLCQPLER
810 820 830 840 850
REGGWPTAAH NNIALIWPSE WQMIQKLFVV DKVIKITRIE VGDVNPSQTQ
860 870 880 890 900
YISEPSLCPD CREGLLCQQQ RDLREYTQAT IYVHKVVDNK KVMKDSAPEL
910 920 930 940 950
NVSSSETEED KEEAKPDGEK DPDFNQSNGS TKRQKTSQQG YVAYQKQVIR
960 970 980 990 1000
RSTRHRKVRG EKALLVSANQ TLKELKIQIM HAFSVAPFDQ NLSIDGKILN
1010 1020 1030 1040 1050
DDCATLGTLG VIPESVILLK ADEPIADYAA MDDVMQVCMP EEGFKGTGLL

GH
Length:1,052
Mass (Da):120,631
Last modified:September 19, 2006 - v2
Checksum:i347520CB80F34303
GO
Isoform 2 (identifier: Q3V0C5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVGPCPFGRRRRRRRRPPGSASATSVKAM

Note: No experimental confirmation available.
Show »
Length:1,080
Mass (Da):123,723
Checksum:i0449068D6093A9CD
GO
Isoform 3 (identifier: Q3V0C5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-390: L → LA
     483-484: EP → GL
     485-1052: Missing.

Show »
Length:485
Mass (Da):55,956
Checksum:i558437C9A7A809FE
GO
Isoform 4 (identifier: Q3V0C5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     483-484: EP → GL
     485-1052: Missing.

Show »
Length:484
Mass (Da):55,884
Checksum:i8ACBDF6704499F90
GO
Isoform 5 (identifier: Q3V0C5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     390-390: L → LA

Show »
Length:1,053
Mass (Da):120,702
Checksum:i503A45728DEE622D
GO

Sequence cautioni

The sequence AAH21769 differs from that shown. Reason: Frameshift at position 377.Curated
The sequence BAD90375 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0204821M → MVGPCPFGRRRRRRRRPPGS ASATSVKAM in isoform 2. 1 Publication1
Alternative sequenceiVSP_020483390L → LA in isoform 3 and isoform 5. 2 Publications1
Alternative sequenceiVSP_020484483 – 484EP → GL in isoform 3 and isoform 4. 2 Publications2
Alternative sequenceiVSP_020485485 – 1052Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST568

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220190 mRNA. Translation: BAD90375.1. Different initiation.
AK133251 mRNA. Translation: BAE21579.1.
AK166158 mRNA. Translation: BAE38603.1.
BC021769 mRNA. Translation: AAH21769.1. Frameshift.
CCDSiCCDS38924.1. [Q3V0C5-1]
RefSeqiNP_570949.2. NM_130879.2. [Q3V0C5-1]
XP_017175495.1. XM_017320006.1. [Q3V0C5-5]
UniGeneiMm.218478.

Genome annotation databases

EnsembliENSMUST00000055131; ENSMUSP00000055016; ENSMUSG00000043411. [Q3V0C5-1]
GeneIDi170707.
KEGGimmu:170707.
UCSCiuc008vjh.1. mouse. [Q3V0C5-2]
uc008vji.1. mouse. [Q3V0C5-3]
uc008vjj.1. mouse. [Q3V0C5-4]
uc008vjl.1. mouse. [Q3V0C5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiUBP48_MOUSE
AccessioniPrimary (citable) accession number: Q3V0C5
Secondary accession number(s): Q3TM39, Q571J9, Q8VDJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: November 22, 2017
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families