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Q3V0C5 (UBP48_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 48

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 48
Ubiquitin thioesterase 48
Ubiquitin-specific-processing protease 48
Gene names
Name:Usp48
Synonyms:Kiaa4202
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1052 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. May be involved in the regulation of NF-kappa-B activation by TNF receptor superfamily via its interactions with RELA and TRAF2. May also play a regulatory role at postsynaptic sites By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with TRAF2 and RELA By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 3 DUSP domains.

Contains 1 ubiquitin-like domain.

Contains 1 USP domain.

Sequence caution

The sequence AAH21769.1 differs from that shown. Reason: Frameshift at position 377.

The sequence BAD90375.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   Molecular functionHydrolase
Protease
Thiol protease
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3V0C5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3V0C5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MVGPCPFGRRRRRRRRPPGSASATSVKAM
Note: No experimental confirmation available.
Isoform 3 (identifier: Q3V0C5-3)

The sequence of this isoform differs from the canonical sequence as follows:
     390-390: L → LA
     483-484: EP → GL
     485-1052: Missing.
Isoform 4 (identifier: Q3V0C5-4)

The sequence of this isoform differs from the canonical sequence as follows:
     483-484: EP → GL
     485-1052: Missing.
Isoform 5 (identifier: Q3V0C5-5)

The sequence of this isoform differs from the canonical sequence as follows:
     390-390: L → LA

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10521052Ubiquitin carboxyl-terminal hydrolase 48
PRO_0000249524

Regions

Domain89 – 420332USP
Domain459 – 55395DUSP 1
Domain568 – 691124DUSP 2
Domain710 – 841132DUSP 3
Domain946 – 102681Ubiquitin-like

Sites

Active site981Nucleophile By similarity
Active site3531Proton acceptor By similarity

Amino acid modifications

Modified residue9031Phosphoserine By similarity
Modified residue9041Phosphoserine By similarity
Modified residue9051Phosphoserine By similarity
Modified residue9731N6-acetyllysine By similarity

Natural variations

Alternative sequence11M → MVGPCPFGRRRRRRRRPPGS ASATSVKAM in isoform 2.
VSP_020482
Alternative sequence3901L → LA in isoform 3 and isoform 5.
VSP_020483
Alternative sequence483 – 4842EP → GL in isoform 3 and isoform 4.
VSP_020484
Alternative sequence485 – 1052568Missing in isoform 3 and isoform 4.
VSP_020485

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified September 19, 2006. Version 2.
Checksum: 347520CB80F34303

FASTA1,052120,631
        10         20         30         40         50         60 
MAPRLQLEKA AWRWAETVRP EEVSQEHIET AYRIWLEPCI RGVCRRNCRG NPNCLVGIGE 

        70         80         90        100        110        120 
HIWLGEIDEN SFHSIDDPNC ERRKKNSFVG LTNLGATCYV NTFLQVWFLN LELRQALYLC 

       130        140        150        160        170        180 
PSTCSDYTKG DGIHGGKDYE PQTICEHLQY LFALLQNSNR RYIDPSGFVK ALGLDTGQQQ 

       190        200        210        220        230        240 
DAQEFSKLFM SLLEDTLSKQ KNPDVRNVVQ QQFCGEYAYV TVCNQCGRES KLVSKFYELE 

       250        260        270        280        290        300 
LNIQGHKQLT DCISEFLKEE RLEGDNRYFC ENCQSKQNAT RKIRLLSLPC TLNLQLMRFV 

       310        320        330        340        350        360 
FDRQTGHKKK LNAYIGFSES LDMEPYVEHK GGSFVYELSA VLIHRGVSAY SGHYIAHVKD 

       370        380        390        400        410        420 
PQSGEWYKFN DEDIEKMEGK KLQLGIEEDL EPSKSQTRKP KCGKGTHCSR NAYMLVYRLQ 

       430        440        450        460        470        480 
AQEKNHTMVQ VPAFLQELVD RDNSKFEEWC VEMAEMRKQS VDKGKAKHEE VKELYQRLPA 

       490        500        510        520        530        540 
GAEPYEFVSL EWLQKWLDES TPTKPIDNNA CLCSHDKLHP DKISIMKRIS EYAANIFYSR 

       550        560        570        580        590        600 
YGGGPRLTVK ALCKDCVVER CRVLRLKNQL NEDYKAVNNL LKSTMKGDGF WVGKSSLRSW 

       610        620        630        640        650        660 
RQLALEQLDE QDGEAEQSNG KINGSTFNKD ESKEEKKEEE EELNFNEDIL CPHGELSISE 

       670        680        690        700        710        720 
NERRLVSQEA WSKLQQYFPK APEFPSYREC CSQCKILERE GEENEALHKM IANEQKTSLP 

       730        740        750        760        770        780 
NLFQDKNRPC LSNWPEDTDA LYIVSHFFLD EWRKFVRKPA RSAPVSSIGN AALLCPHGGL 

       790        800        810        820        830        840 
MFTFPSLTKE DSKLCQPLER REGGWPTAAH NNIALIWPSE WQMIQKLFVV DKVIKITRIE 

       850        860        870        880        890        900 
VGDVNPSQTQ YISEPSLCPD CREGLLCQQQ RDLREYTQAT IYVHKVVDNK KVMKDSAPEL 

       910        920        930        940        950        960 
NVSSSETEED KEEAKPDGEK DPDFNQSNGS TKRQKTSQQG YVAYQKQVIR RSTRHRKVRG 

       970        980        990       1000       1010       1020 
EKALLVSANQ TLKELKIQIM HAFSVAPFDQ NLSIDGKILN DDCATLGTLG VIPESVILLK 

      1030       1040       1050 
ADEPIADYAA MDDVMQVCMP EEGFKGTGLL GH 

« Hide

Isoform 2 [UniParc].

Checksum: 0449068D6093A9CD
Show »

FASTA1,080123,723
Isoform 3 [UniParc].

Checksum: 558437C9A7A809FE
Show »

FASTA48555,956
Isoform 4 [UniParc].

Checksum: 8ACBDF6704499F90
Show »

FASTA48455,884
Isoform 5 [UniParc].

Checksum: 503A45728DEE622D
Show »

FASTA1,053120,702

References

[1]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
Tissue: Embryonic tail.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
Strain: C57BL/6J.
Tissue: Lung and Testis.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-633 (ISOFORM 5).
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK220190 mRNA. Translation: BAD90375.1. Different initiation.
AK133251 mRNA. Translation: BAE21579.1.
AK166158 mRNA. Translation: BAE38603.1.
BC021769 mRNA. Translation: AAH21769.1. Frameshift.
CCDSCCDS38924.1. [Q3V0C5-1]
RefSeqNP_570949.2. NM_130879.2. [Q3V0C5-1]
XP_006538662.1. XM_006538599.1. [Q3V0C5-5]
UniGeneMm.218478.

3D structure databases

ProteinModelPortalQ3V0C5.
SMRQ3V0C5. Positions 84-417, 962-1013.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid228386. 1 interaction.

Protein family/group databases

MEROPSC19.068.

PTM databases

PhosphoSiteQ3V0C5.

Proteomic databases

MaxQBQ3V0C5.
PaxDbQ3V0C5.
PRIDEQ3V0C5.

Protocols and materials databases

DNASU170707.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055131; ENSMUSP00000055016; ENSMUSG00000043411. [Q3V0C5-1]
GeneID170707.
KEGGmmu:170707.
UCSCuc008vjh.1. mouse. [Q3V0C5-2]
uc008vji.1. mouse. [Q3V0C5-3]
uc008vjj.1. mouse. [Q3V0C5-4]
uc008vjl.1. mouse. [Q3V0C5-1]

Organism-specific databases

CTD84196.
MGIMGI:2158502. Usp48.
RougeSearch...

Phylogenomic databases

eggNOGCOG5077.
GeneTreeENSGT00740000115066.
HOGENOMHOG000231639.
HOVERGENHBG080368.
KOK11858.
OMALSNWPED.
PhylomeDBQ3V0C5.
TreeFamTF106280.

Gene expression databases

ArrayExpressQ3V0C5.
BgeeQ3V0C5.
CleanExMM_USP48.
GenevestigatorQ3V0C5.

Family and domain databases

Gene3D3.30.2230.10. 1 hit.
InterProIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00443. UCH. 1 hit.
[Graphical view]
SUPFAMSSF143791. SSF143791. 1 hit.
SSF54236. SSF54236. 1 hit.
PROSITEPS51283. DUSP. 3 hits.
PS50053. UBIQUITIN_2. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSUSP48. mouse.
NextBio370218.
PROQ3V0C5.
SOURCESearch...

Entry information

Entry nameUBP48_MOUSE
AccessionPrimary (citable) accession number: Q3V0C5
Secondary accession number(s): Q3TM39, Q571J9, Q8VDJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: September 19, 2006
Last modified: July 9, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot