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Protein

Homeodomain-interacting protein kinase 4

Gene

Hipk4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein kinase that phosphorylates murine TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter. May act as a corepressor of transcription factors (Potential).Curated

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei136Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: MGI
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • histone phosphorylation Source: MGI
  • peptidyl-serine phosphorylation Source: MGI
  • protein autophosphorylation Source: MGI
  • protein phosphorylation Source: MGI
  • regulation of signal transduction by p53 class mediator Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeodomain-interacting protein kinase 4 (EC:2.7.11.1)
Gene namesi
Name:Hipk4
Synonyms:Gm162
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2685008. Hipk4.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40K → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776041 – 616Homeodomain-interacting protein kinase 4Add BLAST616

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei512PhosphoserineCombined sources1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3V016.
PeptideAtlasiQ3V016.
PRIDEiQ3V016.

PTM databases

iPTMnetiQ3V016.
PhosphoSitePlusiQ3V016.

Expressioni

Tissue specificityi

Expressed at moderate levels in lung and white adipose tissues and weakly in brain and liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000040424.
CleanExiMM_HIPK4.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103990.

Structurei

3D structure databases

ProteinModelPortaliQ3V016.
SMRiQ3V016.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 347Protein kinasePROSITE-ProRule annotationAdd BLAST337

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi379 – 382Poly-Pro4
Compositional biasi398 – 401Poly-Glu4

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
GeneTreeiENSGT00760000119032.
HOGENOMiHOG000090252.
HOVERGENiHBG081604.
InParanoidiQ3V016.
KOiK08826.
OMAiHIIRFLE.
OrthoDBiEOG091G04Y2.
PhylomeDBiQ3V016.
TreeFamiTF105417.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3V016-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATIQSETDC YDIIEVLGKG TFGEVAKGWR RSTGEMVAIK ILKNDAYRSR
60 70 80 90 100
IIKNELKLLR CVRGLDPDEA HVIRFLEFFH DALKFYLVFE LLEQNLFEFQ
110 120 130 140 150
KENNFAPLPA RHIRTVTLQV LRALARLKEL AIIHADLKPE NIMLVDQTRC
160 170 180 190 200
PFRVKVIDFG SASIFSEVRY VKEPYIQSRF YRAPEILLGL PFCEKVDVWS
210 220 230 240 250
LGCVMAELHL GWPLYPGNNE YDQVRYICET QGLPKPHLLH AARKAHHFFK
260 270 280 290 300
RNPHPDATNP WQLKSSADYL AETKVRPLER RKYMLKSLDQ IETVNGGGAV
310 320 330 340 350
SRLSFPDREA LAEHADLKSM VELIKRMLTW ESHERISPSA ALRHPFVSMQ
360 370 380 390 400
QLRSAHEATR YYQLSLRGCR LSLQVDGKPP PPVIASAEDG PPYYRLAEEE
410 420 430 440 450
ETAGLGGVTG SGSFFREDKA PGMQRAIDQL DDLSLQEARR GLWSDTRADM
460 470 480 490 500
VSDMLVPLKV ASTSHRVPDS GPEPILAFYG SRLTGRHKAR KAPAGSKSDS
510 520 530 540 550
NFSNLIRLSQ ASPEDAGPCR GSGWEEGEGR TTSTEPSVIP QREGDGPGIK
560 570 580 590 600
DRPMDAERPG PELFDPSSCP GEWLSEPEWT LEGIRGSRAQ GLPAHHPHPH
610
GPPRTTSFLQ HVGGHH
Length:616
Mass (Da):69,279
Last modified:February 6, 2007 - v2
Checksum:i741CC756379D25C8
GO
Isoform 2 (identifier: Q3V016-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-204: Missing.

Show »
Length:412
Mass (Da):45,642
Checksum:i181F65EB1F897762
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti381P → T in BAF93448 (PubMed:18022393).Curated1
Sequence conflicti381P → T in BAE21689 (PubMed:16141072).Curated1
Sequence conflicti499D → N in BAF93448 (PubMed:18022393).Curated1
Sequence conflicti499D → N in BAE21689 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0230351 – 204Missing in isoform 2. 2 PublicationsAdd BLAST204

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB354583 mRNA. Translation: BAF93448.1.
AK133498 mRNA. Translation: BAE21689.1.
AC158304 Genomic DNA. No translation available.
BC147572 mRNA. Translation: AAI47573.1.
BC147577 mRNA. Translation: AAI47578.1.
CCDSiCCDS21024.2. [Q3V016-1]
RefSeqiNP_001028487.2. NM_001033315.2. [Q3V016-1]
XP_011248824.1. XM_011250522.2. [Q3V016-2]
UniGeneiMm.429716.

Genome annotation databases

EnsembliENSMUST00000037134; ENSMUSP00000043175; ENSMUSG00000040424. [Q3V016-2]
ENSMUST00000108353; ENSMUSP00000103990; ENSMUSG00000040424. [Q3V016-1]
GeneIDi233020.
KEGGimmu:233020.
UCSCiuc009fwl.2. mouse. [Q3V016-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB354583 mRNA. Translation: BAF93448.1.
AK133498 mRNA. Translation: BAE21689.1.
AC158304 Genomic DNA. No translation available.
BC147572 mRNA. Translation: AAI47573.1.
BC147577 mRNA. Translation: AAI47578.1.
CCDSiCCDS21024.2. [Q3V016-1]
RefSeqiNP_001028487.2. NM_001033315.2. [Q3V016-1]
XP_011248824.1. XM_011250522.2. [Q3V016-2]
UniGeneiMm.429716.

3D structure databases

ProteinModelPortaliQ3V016.
SMRiQ3V016.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000103990.

PTM databases

iPTMnetiQ3V016.
PhosphoSitePlusiQ3V016.

Proteomic databases

PaxDbiQ3V016.
PeptideAtlasiQ3V016.
PRIDEiQ3V016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037134; ENSMUSP00000043175; ENSMUSG00000040424. [Q3V016-2]
ENSMUST00000108353; ENSMUSP00000103990; ENSMUSG00000040424. [Q3V016-1]
GeneIDi233020.
KEGGimmu:233020.
UCSCiuc009fwl.2. mouse. [Q3V016-1]

Organism-specific databases

CTDi147746.
MGIiMGI:2685008. Hipk4.

Phylogenomic databases

eggNOGiKOG0667. Eukaryota.
ENOG410XPET. LUCA.
GeneTreeiENSGT00760000119032.
HOGENOMiHOG000090252.
HOVERGENiHBG081604.
InParanoidiQ3V016.
KOiK08826.
OMAiHIIRFLE.
OrthoDBiEOG091G04Y2.
PhylomeDBiQ3V016.
TreeFamiTF105417.

Miscellaneous databases

PROiQ3V016.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040424.
CleanExiMM_HIPK4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHIPK4_MOUSE
AccessioniPrimary (citable) accession number: Q3V016
Secondary accession number(s): A8R3X8, B9EJV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.