Reviewed, UniProtKB/Swiss-Prot Q3UYH7 (ARBK2_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-adrenergic receptor kinase 2
      Short name=Beta-ARK-2
    EC=2.7.11.15

Gene names

Name:

Adrbk2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	688 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
Catalytic activity	ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from direct assay. Source: MGI beta-adrenergic receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 688	688	Beta-adrenergic receptor kinase 2		PRO_0000260828
Regions
	Domain	54 – 175	122	RGS
	Domain	191 – 453	263	Protein kinase
	Domain	454 – 521	68	AGC-kinase C-terminal
	Domain	558 – 652	95	PH
	Nucleotide binding	197 – 205	9	ATP By similarity
	Region	1 – 190	190	N-terminal
Sites
	Active site	317	1	Proton acceptor By similarity
	Binding site	220	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3UYH7-1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: DF58808FD6136EAF	FASTA	688	79,643
	10 20 30 40 50 60 MADLEAVLAD VSYLMAMEKS KTAPAARASK KVVLPEPSIR SVMQRYLAER NEITFDKIFN 70 80 90 100 110 120 QKIGFLLFKD FCLNEIGEAV PQVKFYEEIK EYEKLDNEED RLRRSRQMYD AYIMRELLSS 130 140 150 160 170 180 THQFSKQAVE HVQSHLSKKQ VTATLFQPYI EEICESLRGD IFQKFMESDK FTRFCQWKNV 190 200 210 220 230 240 ELNIHLSMND FSVHRIIGRG GFGEVYGCRK ADTGKMYAMK CLDKKRVKMK QGETLALNER 250 260 270 280 290 300 IMLSLVSTGD CPFIVCMTYA FHTPDKLCFI LDLMNGGDMH YHLSQHGVFS EKEMRFYASE 310 320 330 340 350 360 IILGLEHMHT CFVVYRDLNP ANILLDEYGH VRISDLGLAC DFSKKKPHAS VGTHGYMAPE 370 380 390 400 410 420 VLQKGTCYDS SADWFSLGCM LFKLLRGHSP FRQHKTKDKH EIDRMTLTVN VQLPDAFSPE 430 440 450 460 470 480 LRSLLEGLLQ RDVSQRLGCG GGGARELKEH IFFKGIDWQH VYLRKYPPPL IPPRGEVNAA 490 500 510 520 530 540 DAFDIGSFDE EDTKGIKLLD CDQDLYKNFP LVISERWQQE VVETIYDAVN ADTDKIEARR 550 560 570 580 590 600 KAKNKQLGQE EDYAMGKDCI MHGYMLKLGN PFLTQWQRRY FYLFPNRLEW RGEGESRQSL 610 620 630 640 650 660 LTMEQIMSVE ETQIKDRKCI LLRIKGGKQF VLQCESDPEF AQWLKELTCT FNEAQRLLRR 670 680 APKFLNKPRA AILEFSKPPL CHRNSSGL

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Medulla oblongata.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK134672 mRNA. Translation: BAE22235.1.
IPI	IPI00622878.
RefSeq	NP_796052.2.
UniGene	Mm.285619
3D structure databases
HSSP	HSSP built from PDB template 2BCJ based on UniProtKB P21146.
SMR	Q3UYH7. Positions 28-667.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3UYH7.
PTM databases
PhosphoSite	Q3UYH7.
Proteomic databases
PRIDE	Q3UYH7.
Genome annotation databases
Ensembl	ENSMUST00000065167; ENSMUSP00000070445; ENSMUSG00000042249; Mus musculus. [Genome view]
GeneID	320129.
UCSC	uc008ytt.1. mouse.
Organism-specific databases
CTD	320129.
MGI	MGI:87941. Adrbk2.
Phylogenomic databases
eggNOG	roNOG09946.
HOGENOM	HBG443643.
HOVERGEN	Q3UYH7.
InParanoid	Q3UYH7.
Enzyme and pathway databases
BRENDA	2.7.11.15. 244.
Gene expression databases
ArrayExpress	Q3UYH7.
Bgee	Q3UYH7.
CleanEx	MM_ADRBK2.
Genevestigator	Q3UYH7.
GermOnline	ENSMUSG00000042249. Mus musculus.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR015743. B-adrenergic_rcpt_kinase. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR017442. Se/Thr_prot_kinase-like_dom. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
PANTHER	PTHR22985:SF5. BARK. 1 hit.
Pfam	PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00233. PH. 1 hit. SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	396088.
SOURCE	Search...

Entry information

Entry name

ARBK2_MOUSE

Accession

Primary (citable) accession number: Q3UYH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	October 11, 2005
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)