Q3UXL4 (KIZ_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Centrosomal protein kizuna

Alternative name(s):
Polo-like kinase 1 substrate 1

Gene names

Name:	Plk1s1
Synonyms:	Gm114, Kiz

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole By similarity.
Subunit structure	Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2 By similarity.
Subcellular location	Cytoplasm › cytoskeleton › centrosome By similarity. Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively By similarity.
Post-translational modification	Phosphorylation at Thr-391 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization By similarity.
Sequence similarities	Belongs to the kizuna family.
Sequence caution	The sequence CAM27275.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	spindle organization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from direct assay PubMed 18423593. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Centrosomal protein kizuna		PRO_0000301852
Regions
	Coiled coil	29 – 57	29	Potential
	Coiled coil	102 – 132	31	Potential
Amino acid modifications
	Modified residue	391	1	Phosphothreonine; by PLK1 By similarity
	Modified residue	667	1	Phosphoserine By similarity
	Modified residue	670	1	Phosphoserine By similarity
	Modified residue	672	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3UXL4 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 71DDC7CB94FD6EBF	FASTA	695	76,542
	10 20 30 40 50 60 MPRGRGGGGG GLRQASATSA PLASPSYYER VGQLQQALRD SEKKRLDLED KLYEYNKSDK 70 80 90 100 110 120 CRAKLKCAKL KKYLKEVCES ERRAQVRNQG YLKKFECVQT YVEHLTTNTE KLQKLKTEYE 130 140 150 160 170 180 AEVKRMRLLS KDSLGMSDED GAKDAVQAGI NSGTAMSRGL YQPATIFMGR QMSAISSIED 190 200 210 220 230 240 FSTELKSNQP TKNFSISDPH SHQQTAQSSC VTDSCVVQTN GDTQCLNKSD KIHGKTSLQT 250 260 270 280 290 300 GEKAPVTSYV LSAEEQTHCL EIGSSTQHSK SNLSEGRKSA ELHSSLQERL SPENSITDLK 310 320 330 340 350 360 CDSSSRSEGS DREILTQEHI EVREERAGPL VPMMAASEHC TSAKKWAGEK HSAWEASSDD 370 380 390 400 410 420 LDHGDSKSQK AVLKHEEEQE EGSSCSSSDL TVSVSEDDLI LESLAALSNP GAKMAGKDGM 430 440 450 460 470 480 QALRAAHTEP RQDSLFTEYV LQTQSFPDSK REPSPDSPRQ PEKVPDCHLL KTRGQCMKEH 490 500 510 520 530 540 DNSLKEEATT LLKKVLTEEC DHRSAIHSNE SSCSMPSILN DNNGIKEAKP ALRLNSVLTR 550 560 570 580 590 600 EQEVSSGCGD ESKEESIAAA PGTGHREADL VPSELFQFSL GAAMKETKAY QLLKKSTLQD 610 620 630 640 650 660 NSNQAEERFE SQFSGLDIGG SMFTTKTAHK IASEASFSSS EGSPLSRHES KRDPVTTIKS 670 680 690 NAFWGESDDS NSEIEAALRP RDHDMPDDFD DFYDT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Muellerian duct.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 73-82; 76-87 AND 132-158, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK135486 mRNA. Translation: BAE22549.1. AL929215 Genomic DNA. Translation: CAM27275.1. Sequence problems. AL929215 Genomic DNA. Translation: CAM27276.1. BC150844 mRNA. Translation: AAI50845.1. BC150845 mRNA. Translation: AAI50846.1.
IPI	IPI00349838.
RefSeq	NP_001028470.1. NM_001033298.3.
UniGene	Mm.292352.
3D structure databases
ProteinModelPortal	Q3UXL4.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3UXL4. 1 interaction.
PTM databases
PhosphoSite	Q3UXL4.
Proteomic databases
PRIDE	Q3UXL4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000099278; ENSMUSP00000096884; ENSMUSG00000074749. ENSMUST00000156232; ENSMUSP00000121952; ENSMUSG00000074749.
GeneID	228730.
KEGG	mmu:228730.
UCSC	uc008mso.1. mouse.
Organism-specific databases
CTD	55857.
MGI	MGI:2684960. Plk1s1.
Phylogenomic databases
eggNOG	NOG25010.
GeneTree	ENSGT00390000010121.
HOGENOM	HOG000060289.
InParanoid	B2RX19.
KO	K16539.
OMA	SRGLYQP.
OrthoDB	EOG40K7ZF.
Gene expression databases
Bgee	Q3UXL4.
Genevestigator	Q3UXL4.
Family and domain databases
InterPro	IPR026742. Centrosomal_kizuma. [Graphical view]
PANTHER	PTHR16299. PTHR16299. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	PLK1S1. mouse.
NextBio	379092.
SOURCE	Search...

Entry information

Entry name

KIZ_MOUSE

Accession

Primary (citable) accession number: Q3UXL4
Secondary accession number(s): A2AUS7, B2RX19

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 11, 2005
Last modified:	March 6, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program