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Q3UWA6

- GUC2C_MOUSE

UniProt

Q3UWA6 - GUC2C_MOUSE

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Protein
Heat-stable enterotoxin receptor
Gene
Gucy2c, Guc2c
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin By similarity.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. GTP binding Source: UniProtKB-KW
  3. guanylate cyclase activity Source: MGI
  4. protein kinase activity Source: InterPro
  5. toxic substance binding Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. cGMP biosynthetic process Source: MGI
  2. intracellular signal transduction Source: InterPro
  3. regulation of cell proliferation Source: MGI
  4. response to toxic substance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heat-stable enterotoxin receptor (EC:4.6.1.2)
Short name:
STA receptor
Alternative name(s):
Guanylyl cyclase C
Short name:
GC-C
Intestinal guanylate cyclase
Gene namesi
Name:Gucy2c
Synonyms:Guc2c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:106903. Gucy2c.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein
Note: The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini20 – 433414Extracellular Reviewed prediction
Add
BLAST
Transmembranei434 – 45421Helical; Reviewed prediction
Add
BLAST
Topological domaini455 – 1072618Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 10721053Heat-stable enterotoxin receptor
PRO_0000280396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi32 – 321N-linked (GlcNAc...) Reviewed prediction
Glycosylationi75 – 751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi179 – 1791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi188 – 1881N-linked (GlcNAc...) Reviewed prediction
Glycosylationi195 – 1951N-linked (GlcNAc...) Reviewed prediction
Glycosylationi284 – 2841N-linked (GlcNAc...) Reviewed prediction
Glycosylationi307 – 3071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi345 – 3451N-linked (GlcNAc...) Reviewed prediction
Glycosylationi402 – 4021N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Glycosylation at Asn-75 and/or Asn-79 is required for interaction with VIP36 while glycosylation at Asn-345 and Asn-402 modulates ligand-mediated GC-C activation By similarity.

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ3UWA6.
PRIDEiQ3UWA6.

PTM databases

PhosphoSiteiQ3UWA6.

Expressioni

Gene expression databases

BgeeiQ3UWA6.
CleanExiMM_GUCY2C.
GenevestigatoriQ3UWA6.

Interactioni

Subunit structurei

Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 By similarity.

Protein-protein interaction databases

BioGridi200125. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ3UWA6.
SMRiQ3UWA6. Positions 119-374, 540-748, 813-988.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini489 – 748260Protein kinase
Add
BLAST
Domaini823 – 953131Guanylate cyclase
Add
BLAST

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2114.
GeneTreeiENSGT00730000110520.
HOGENOMiHOG000112833.
HOVERGENiHBG106967.
InParanoidiQ3UWA6.
KOiK12320.
OMAiAYHDAVL.
OrthoDBiEOG72VH58.
PhylomeDBiQ3UWA6.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3UWA6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTSLLGLAVR LLLFQPALMV FWASQVRQNC RNGSYEISVL MMDNSAYKEP     50
MQNLREAVEE GLDIVRKRLR EADLNVTVNA TFIYSDGLIH KSGDCRSSTC 100
EGLDLLREIT RDHKMGCALM GPSCTYSTFQ MYLDTELNYP MISAGSYGLS 150
CDYKETLTRI LPPARKLMYF LVDFWKVNNA SFKPFSWNSS YVYKNGSEPE 200
DCFWYLNALE AGVSYFSEVL NFKDVLRRSE QFQEILTGHN RKSNVIVMCG 250
TPESFYDVKG DLQVAEDTVV ILVDLFSNHY FEENTTAPEY MDNVLVLTLP 300
SEQSTSNTSV AERFSSGRSD FSLAYLEGTL LFGHMLQTFL ENGENVTGPK 350
FARAFRNLTF QGFAGPVTLD DSGDIDNIMS LLYVSLDTRK YKVLMKYDTH 400
KNKTIPVAEN PNFIWKNHKL PNDVPGLGPQ ILMIAVFTLT GILVVLLLIA 450
LLVLRKYRRD HALRQKKWSH IPSENIFPLE TNETNHISLK IDDDRRRDTI 500
QRVRQCKYDK KKVILKDLKH SDGNFSEKQK IDLNKLLQSD YYNLTKFYGT 550
VKLDTRIFGV VEYCERGSLR EVLNDTISYP DGTFMDWEFK ISVLNDIAKG 600
MSYLHSSKIE VHGRLKSTNC VVDSRMVVKI TDFGCNSILP PKKDLWTAPE 650
HLRQATISQK GDVYSFAIIA QEIILRKETF YTLSCRDHNE KIFRVENSYG 700
KPFRPDLFLE TADEKELEVY LLVKSCWEED PEKRPDFKKI ESTLAKIFGL 750
FHDQKNESYM DTLIRRLQLY SRNLEHLVEE RTQLYKAERD RADHLNFMLL 800
PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST PMEVVDMLND 850
IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL 900
SFIGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS 950
RMESTGLPLR IHMSSSTITI LKRTDCQFLY EVRGETYLKG RGTETTYWLT 1000
GMKDQEYNLP SPPTVENQQR LQTEFSDMIV SALQKRQASG KKSRRPTRVA 1050
SYKKGFLEYM QLNNSDHDST YF 1072
Length:1,072
Mass (Da):123,232
Last modified:October 11, 2005 - v1
Checksum:i7129508166E7790A
GO
Isoform 2 (identifier: Q3UWA6-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     512-535: Missing.

Note: No experimental confirmation available.

Show »
Length:1,048
Mass (Da):120,452
Checksum:i3F3B0D2056C5B79A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei512 – 53524Missing in isoform 2.
VSP_023652Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti381 – 3811L → F in AAH34064. 1 Publication
Sequence conflicti969 – 9691T → A in AAH34064. 1 Publication
Sequence conflicti969 – 9691T → A in AAH99968. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK136496 mRNA. Translation: BAE23010.1.
BC034064 mRNA. Translation: AAH34064.1.
BC099968 mRNA. Translation: AAH99968.1.
CCDSiCCDS20653.1. [Q3UWA6-2]
CCDS51941.1. [Q3UWA6-1]
RefSeqiNP_001120790.1. NM_001127318.1. [Q3UWA6-1]
NP_659504.2. NM_145067.3. [Q3UWA6-2]
UniGeneiMm.222729.

Genome annotation databases

EnsembliENSMUST00000032338; ENSMUSP00000032338; ENSMUSG00000042638. [Q3UWA6-1]
ENSMUST00000078095; ENSMUSP00000077236; ENSMUSG00000042638. [Q3UWA6-2]
GeneIDi14917.
KEGGimmu:14917.
UCSCiuc009emb.2. mouse. [Q3UWA6-2]
uc009emc.2. mouse. [Q3UWA6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK136496 mRNA. Translation: BAE23010.1 .
BC034064 mRNA. Translation: AAH34064.1 .
BC099968 mRNA. Translation: AAH99968.1 .
CCDSi CCDS20653.1. [Q3UWA6-2 ]
CCDS51941.1. [Q3UWA6-1 ]
RefSeqi NP_001120790.1. NM_001127318.1. [Q3UWA6-1 ]
NP_659504.2. NM_145067.3. [Q3UWA6-2 ]
UniGenei Mm.222729.

3D structure databases

ProteinModelPortali Q3UWA6.
SMRi Q3UWA6. Positions 119-374, 540-748, 813-988.
ModBasei Search...

Protein-protein interaction databases

BioGridi 200125. 2 interactions.

PTM databases

PhosphoSitei Q3UWA6.

Proteomic databases

MaxQBi Q3UWA6.
PRIDEi Q3UWA6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032338 ; ENSMUSP00000032338 ; ENSMUSG00000042638 . [Q3UWA6-1 ]
ENSMUST00000078095 ; ENSMUSP00000077236 ; ENSMUSG00000042638 . [Q3UWA6-2 ]
GeneIDi 14917.
KEGGi mmu:14917.
UCSCi uc009emb.2. mouse. [Q3UWA6-2 ]
uc009emc.2. mouse. [Q3UWA6-1 ]

Organism-specific databases

CTDi 2984.
MGIi MGI:106903. Gucy2c.

Phylogenomic databases

eggNOGi COG2114.
GeneTreei ENSGT00730000110520.
HOGENOMi HOG000112833.
HOVERGENi HBG106967.
InParanoidi Q3UWA6.
KOi K12320.
OMAi AYHDAVL.
OrthoDBi EOG72VH58.
PhylomeDBi Q3UWA6.
TreeFami TF106338.

Miscellaneous databases

NextBioi 287217.
PROi Q3UWA6.
SOURCEi Search...

Gene expression databases

Bgeei Q3UWA6.
CleanExi MM_GUCY2C.
Genevestigatori Q3UWA6.

Family and domain databases

Gene3Di 3.30.70.1230. 1 hit.
InterProi IPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view ]
SMARTi SM00044. CYCc. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Cecum.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-1072 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Colon and Pancreas.

Entry informationi

Entry nameiGUC2C_MOUSE
AccessioniPrimary (citable) accession number: Q3UWA6
Secondary accession number(s): Q499D2, Q8JZQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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