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Q3UWA6 (GUC2C_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Heat-stable enterotoxin receptor

Short name=STA receptor
EC=4.6.1.2
Alternative name(s):
Guanylyl cyclase C
Short name=GC-C
Intestinal guanylate cyclase
Gene names
Name:Gucy2c
Synonyms:Guc2c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1072 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin By similarity.

Catalytic activity

GTP = 3',5'-cyclic GMP + diphosphate.

Subunit structure

Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 By similarity.

Subcellular location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein. Note: The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form By similarity.

Domain

The protein kinase domain is predicted to be catalytically inactive.

Post-translational modification

Glycosylation at Asn-75 and/or Asn-79 is required for interaction with VIP36 while glycosylation at Asn-345 and Asn-402 modulates ligand-mediated GC-C activation By similarity.

Sequence similarities

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Contains 1 guanylate cyclase domain.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3UWA6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3UWA6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     512-535: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 10721053Heat-stable enterotoxin receptor
PRO_0000280396

Regions

Topological domain20 – 433414Extracellular Potential
Transmembrane434 – 45421Helical; Potential
Topological domain455 – 1072618Cytoplasmic Potential
Domain489 – 748260Protein kinase
Domain823 – 953131Guanylate cyclase

Amino acid modifications

Glycosylation321N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Glycosylation1881N-linked (GlcNAc...) Potential
Glycosylation1951N-linked (GlcNAc...) Potential
Glycosylation2841N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence512 – 53524Missing in isoform 2.
VSP_023652

Experimental info

Sequence conflict3811L → F in AAH34064. Ref.2
Sequence conflict9691T → A in AAH34064. Ref.2
Sequence conflict9691T → A in AAH99968. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 7129508166E7790A

FASTA1,072123,232
        10         20         30         40         50         60 
MTSLLGLAVR LLLFQPALMV FWASQVRQNC RNGSYEISVL MMDNSAYKEP MQNLREAVEE 

        70         80         90        100        110        120 
GLDIVRKRLR EADLNVTVNA TFIYSDGLIH KSGDCRSSTC EGLDLLREIT RDHKMGCALM 

       130        140        150        160        170        180 
GPSCTYSTFQ MYLDTELNYP MISAGSYGLS CDYKETLTRI LPPARKLMYF LVDFWKVNNA 

       190        200        210        220        230        240 
SFKPFSWNSS YVYKNGSEPE DCFWYLNALE AGVSYFSEVL NFKDVLRRSE QFQEILTGHN 

       250        260        270        280        290        300 
RKSNVIVMCG TPESFYDVKG DLQVAEDTVV ILVDLFSNHY FEENTTAPEY MDNVLVLTLP 

       310        320        330        340        350        360 
SEQSTSNTSV AERFSSGRSD FSLAYLEGTL LFGHMLQTFL ENGENVTGPK FARAFRNLTF 

       370        380        390        400        410        420 
QGFAGPVTLD DSGDIDNIMS LLYVSLDTRK YKVLMKYDTH KNKTIPVAEN PNFIWKNHKL 

       430        440        450        460        470        480 
PNDVPGLGPQ ILMIAVFTLT GILVVLLLIA LLVLRKYRRD HALRQKKWSH IPSENIFPLE 

       490        500        510        520        530        540 
TNETNHISLK IDDDRRRDTI QRVRQCKYDK KKVILKDLKH SDGNFSEKQK IDLNKLLQSD 

       550        560        570        580        590        600 
YYNLTKFYGT VKLDTRIFGV VEYCERGSLR EVLNDTISYP DGTFMDWEFK ISVLNDIAKG 

       610        620        630        640        650        660 
MSYLHSSKIE VHGRLKSTNC VVDSRMVVKI TDFGCNSILP PKKDLWTAPE HLRQATISQK 

       670        680        690        700        710        720 
GDVYSFAIIA QEIILRKETF YTLSCRDHNE KIFRVENSYG KPFRPDLFLE TADEKELEVY 

       730        740        750        760        770        780 
LLVKSCWEED PEKRPDFKKI ESTLAKIFGL FHDQKNESYM DTLIRRLQLY SRNLEHLVEE 

       790        800        810        820        830        840 
RTQLYKAERD RADHLNFMLL PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST 

       850        860        870        880        890        900 
PMEVVDMLND IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL 

       910        920        930        940        950        960 
SFIGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS RMESTGLPLR 

       970        980        990       1000       1010       1020 
IHMSSSTITI LKRTDCQFLY EVRGETYLKG RGTETTYWLT GMKDQEYNLP SPPTVENQQR 

      1030       1040       1050       1060       1070 
LQTEFSDMIV SALQKRQASG KKSRRPTRVA SYKKGFLEYM QLNNSDHDST YF 

« Hide

Isoform 2 [UniParc].

Checksum: 3F3B0D2056C5B79A
Show »

FASTA1,048120,452

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Cecum.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-1072 (ISOFORM 1).
Strain: FVB/N.
Tissue: Colon and Pancreas.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK136496 mRNA. Translation: BAE23010.1.
BC034064 mRNA. Translation: AAH34064.1.
BC099968 mRNA. Translation: AAH99968.1.
CCDSCCDS20653.1. [Q3UWA6-2]
CCDS51941.1. [Q3UWA6-1]
RefSeqNP_001120790.1. NM_001127318.1. [Q3UWA6-1]
NP_659504.2. NM_145067.3. [Q3UWA6-2]
UniGeneMm.222729.

3D structure databases

ProteinModelPortalQ3UWA6.
SMRQ3UWA6. Positions 119-374, 540-748, 813-988.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200125. 2 interactions.

PTM databases

PhosphoSiteQ3UWA6.

Proteomic databases

MaxQBQ3UWA6.
PRIDEQ3UWA6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032338; ENSMUSP00000032338; ENSMUSG00000042638. [Q3UWA6-1]
ENSMUST00000078095; ENSMUSP00000077236; ENSMUSG00000042638. [Q3UWA6-2]
GeneID14917.
KEGGmmu:14917.
UCSCuc009emb.2. mouse. [Q3UWA6-2]
uc009emc.2. mouse. [Q3UWA6-1]

Organism-specific databases

CTD2984.
MGIMGI:106903. Gucy2c.

Phylogenomic databases

eggNOGCOG2114.
GeneTreeENSGT00730000110520.
HOGENOMHOG000112833.
HOVERGENHBG106967.
InParanoidQ3UWA6.
KOK12320.
OMAAYHDAVL.
OrthoDBEOG72VH58.
PhylomeDBQ3UWA6.
TreeFamTF106338.

Gene expression databases

BgeeQ3UWA6.
CleanExMM_GUCY2C.
GenevestigatorQ3UWA6.

Family and domain databases

Gene3D3.30.70.1230. 1 hit.
InterProIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio287217.
PROQ3UWA6.
SOURCESearch...

Entry information

Entry nameGUC2C_MOUSE
AccessionPrimary (citable) accession number: Q3UWA6
Secondary accession number(s): Q499D2, Q8JZQ6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 11, 2005
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot