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Q3UWA6

- GUC2C_MOUSE

UniProt

Q3UWA6 - GUC2C_MOUSE

Protein

Heat-stable enterotoxin receptor

Gene

Gucy2c

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin By similarity.By similarity

    Catalytic activityi

    GTP = 3',5'-cyclic GMP + diphosphate.

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. GTP binding Source: UniProtKB-KW
    3. guanylate cyclase activity Source: MGI
    4. protein kinase activity Source: InterPro
    5. toxic substance binding Source: Ensembl

    GO - Biological processi

    1. cGMP biosynthetic process Source: MGI
    2. intracellular signal transduction Source: InterPro
    3. regulation of cell proliferation Source: MGI
    4. response to toxic substance Source: MGI

    Keywords - Molecular functioni

    Lyase, Receptor

    Keywords - Biological processi

    cGMP biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heat-stable enterotoxin receptor (EC:4.6.1.2)
    Short name:
    STA receptor
    Alternative name(s):
    Guanylyl cyclase C
    Short name:
    GC-C
    Intestinal guanylate cyclase
    Gene namesi
    Name:Gucy2c
    Synonyms:Guc2c
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:106903. Gucy2c.

    Subcellular locationi

    Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein
    Note: The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form.By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 10721053Heat-stable enterotoxin receptorPRO_0000280396Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi32 – 321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi195 – 1951N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi402 – 4021N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylation at Asn-75 and/or Asn-79 is required for interaction with VIP36 while glycosylation at Asn-345 and Asn-402 modulates ligand-mediated GC-C activation.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ3UWA6.
    PRIDEiQ3UWA6.

    PTM databases

    PhosphoSiteiQ3UWA6.

    Expressioni

    Gene expression databases

    BgeeiQ3UWA6.
    CleanExiMM_GUCY2C.
    GenevestigatoriQ3UWA6.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200125. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UWA6.
    SMRiQ3UWA6. Positions 119-374, 540-748, 813-988.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini20 – 433414ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini455 – 1072618CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei434 – 45421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini489 – 748260Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini823 – 953131Guanylate cyclasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The protein kinase domain is predicted to be catalytically inactive.

    Sequence similaritiesi

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
    Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2114.
    GeneTreeiENSGT00730000110520.
    HOGENOMiHOG000112833.
    HOVERGENiHBG106967.
    InParanoidiQ3UWA6.
    KOiK12320.
    OMAiAYHDAVL.
    OrthoDBiEOG72VH58.
    PhylomeDBiQ3UWA6.
    TreeFamiTF106338.

    Family and domain databases

    Gene3Di3.30.70.1230. 1 hit.
    InterProiIPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view]
    SMARTiSM00044. CYCc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3UWA6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTSLLGLAVR LLLFQPALMV FWASQVRQNC RNGSYEISVL MMDNSAYKEP     50
    MQNLREAVEE GLDIVRKRLR EADLNVTVNA TFIYSDGLIH KSGDCRSSTC 100
    EGLDLLREIT RDHKMGCALM GPSCTYSTFQ MYLDTELNYP MISAGSYGLS 150
    CDYKETLTRI LPPARKLMYF LVDFWKVNNA SFKPFSWNSS YVYKNGSEPE 200
    DCFWYLNALE AGVSYFSEVL NFKDVLRRSE QFQEILTGHN RKSNVIVMCG 250
    TPESFYDVKG DLQVAEDTVV ILVDLFSNHY FEENTTAPEY MDNVLVLTLP 300
    SEQSTSNTSV AERFSSGRSD FSLAYLEGTL LFGHMLQTFL ENGENVTGPK 350
    FARAFRNLTF QGFAGPVTLD DSGDIDNIMS LLYVSLDTRK YKVLMKYDTH 400
    KNKTIPVAEN PNFIWKNHKL PNDVPGLGPQ ILMIAVFTLT GILVVLLLIA 450
    LLVLRKYRRD HALRQKKWSH IPSENIFPLE TNETNHISLK IDDDRRRDTI 500
    QRVRQCKYDK KKVILKDLKH SDGNFSEKQK IDLNKLLQSD YYNLTKFYGT 550
    VKLDTRIFGV VEYCERGSLR EVLNDTISYP DGTFMDWEFK ISVLNDIAKG 600
    MSYLHSSKIE VHGRLKSTNC VVDSRMVVKI TDFGCNSILP PKKDLWTAPE 650
    HLRQATISQK GDVYSFAIIA QEIILRKETF YTLSCRDHNE KIFRVENSYG 700
    KPFRPDLFLE TADEKELEVY LLVKSCWEED PEKRPDFKKI ESTLAKIFGL 750
    FHDQKNESYM DTLIRRLQLY SRNLEHLVEE RTQLYKAERD RADHLNFMLL 800
    PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST PMEVVDMLND 850
    IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL 900
    SFIGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS 950
    RMESTGLPLR IHMSSSTITI LKRTDCQFLY EVRGETYLKG RGTETTYWLT 1000
    GMKDQEYNLP SPPTVENQQR LQTEFSDMIV SALQKRQASG KKSRRPTRVA 1050
    SYKKGFLEYM QLNNSDHDST YF 1072
    Length:1,072
    Mass (Da):123,232
    Last modified:October 11, 2005 - v1
    Checksum:i7129508166E7790A
    GO
    Isoform 2 (identifier: Q3UWA6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         512-535: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:1,048
    Mass (Da):120,452
    Checksum:i3F3B0D2056C5B79A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti381 – 3811L → F in AAH34064. (PubMed:15489334)Curated
    Sequence conflicti969 – 9691T → A in AAH34064. (PubMed:15489334)Curated
    Sequence conflicti969 – 9691T → A in AAH99968. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei512 – 53524Missing in isoform 2. 1 PublicationVSP_023652Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK136496 mRNA. Translation: BAE23010.1.
    BC034064 mRNA. Translation: AAH34064.1.
    BC099968 mRNA. Translation: AAH99968.1.
    CCDSiCCDS20653.1. [Q3UWA6-2]
    CCDS51941.1. [Q3UWA6-1]
    RefSeqiNP_001120790.1. NM_001127318.1. [Q3UWA6-1]
    NP_659504.2. NM_145067.3. [Q3UWA6-2]
    UniGeneiMm.222729.

    Genome annotation databases

    EnsembliENSMUST00000032338; ENSMUSP00000032338; ENSMUSG00000042638. [Q3UWA6-1]
    ENSMUST00000078095; ENSMUSP00000077236; ENSMUSG00000042638. [Q3UWA6-2]
    GeneIDi14917.
    KEGGimmu:14917.
    UCSCiuc009emb.2. mouse. [Q3UWA6-2]
    uc009emc.2. mouse. [Q3UWA6-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK136496 mRNA. Translation: BAE23010.1 .
    BC034064 mRNA. Translation: AAH34064.1 .
    BC099968 mRNA. Translation: AAH99968.1 .
    CCDSi CCDS20653.1. [Q3UWA6-2 ]
    CCDS51941.1. [Q3UWA6-1 ]
    RefSeqi NP_001120790.1. NM_001127318.1. [Q3UWA6-1 ]
    NP_659504.2. NM_145067.3. [Q3UWA6-2 ]
    UniGenei Mm.222729.

    3D structure databases

    ProteinModelPortali Q3UWA6.
    SMRi Q3UWA6. Positions 119-374, 540-748, 813-988.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200125. 2 interactions.

    PTM databases

    PhosphoSitei Q3UWA6.

    Proteomic databases

    MaxQBi Q3UWA6.
    PRIDEi Q3UWA6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032338 ; ENSMUSP00000032338 ; ENSMUSG00000042638 . [Q3UWA6-1 ]
    ENSMUST00000078095 ; ENSMUSP00000077236 ; ENSMUSG00000042638 . [Q3UWA6-2 ]
    GeneIDi 14917.
    KEGGi mmu:14917.
    UCSCi uc009emb.2. mouse. [Q3UWA6-2 ]
    uc009emc.2. mouse. [Q3UWA6-1 ]

    Organism-specific databases

    CTDi 2984.
    MGIi MGI:106903. Gucy2c.

    Phylogenomic databases

    eggNOGi COG2114.
    GeneTreei ENSGT00730000110520.
    HOGENOMi HOG000112833.
    HOVERGENi HBG106967.
    InParanoidi Q3UWA6.
    KOi K12320.
    OMAi AYHDAVL.
    OrthoDBi EOG72VH58.
    PhylomeDBi Q3UWA6.
    TreeFami TF106338.

    Miscellaneous databases

    NextBioi 287217.
    PROi Q3UWA6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3UWA6.
    CleanExi MM_GUCY2C.
    Genevestigatori Q3UWA6.

    Family and domain databases

    Gene3Di 3.30.70.1230. 1 hit.
    InterProi IPR001054. A/G_cyclase.
    IPR018297. A/G_cyclase_CS.
    IPR001828. ANF_lig-bd_rcpt.
    IPR011009. Kinase-like_dom.
    IPR028082. Peripla_BP_I.
    IPR000719. Prot_kinase_dom.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00211. Guanylate_cyc. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    [Graphical view ]
    SMARTi SM00044. CYCc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    SSF55073. SSF55073. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00452. GUANYLATE_CYCLASE_1. 1 hit.
    PS50125. GUANYLATE_CYCLASE_2. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Cecum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-1072 (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Colon and Pancreas.

    Entry informationi

    Entry nameiGUC2C_MOUSE
    AccessioniPrimary (citable) accession number: Q3UWA6
    Secondary accession number(s): Q499D2, Q8JZQ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 20, 2007
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3