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Protein

Heat-stable enterotoxin receptor

Gene

Gucy2c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin (By similarity).By similarity

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: MGI
  • protein kinase activity Source: InterPro
  • toxic substance binding Source: Ensembl

GO - Biological processi

  • cGMP biosynthetic process Source: MGI
  • intracellular signal transduction Source: InterPro
  • regulation of cell proliferation Source: MGI
  • response to toxic substance Source: MGI
  • signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Receptor

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat-stable enterotoxin receptor (EC:4.6.1.2)
Short name:
STA receptor
Alternative name(s):
Guanylyl cyclase C
Short name:
GC-C
Intestinal guanylate cyclase
Gene namesi
Name:Gucy2c
Synonyms:Guc2c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:106903. Gucy2c.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 433ExtracellularSequence analysisAdd BLAST414
Transmembranei434 – 454HelicalSequence analysisAdd BLAST21
Topological domaini455 – 1072CytoplasmicSequence analysisAdd BLAST618

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000028039620 – 1072Heat-stable enterotoxin receptorAdd BLAST1053

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...)Sequence analysis1
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi79N-linked (GlcNAc...)Sequence analysis1
Glycosylationi179N-linked (GlcNAc...)Sequence analysis1
Glycosylationi188N-linked (GlcNAc...)Sequence analysis1
Glycosylationi195N-linked (GlcNAc...)Sequence analysis1
Glycosylationi284N-linked (GlcNAc...)Sequence analysis1
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi345N-linked (GlcNAc...)Sequence analysis1
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylation at Asn-75 and/or Asn-79 is required for interaction with VIP36 while glycosylation at Asn-345 and Asn-402 modulates ligand-mediated GC-C activation.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3UWA6.
PeptideAtlasiQ3UWA6.
PRIDEiQ3UWA6.

PTM databases

iPTMnetiQ3UWA6.
PhosphoSitePlusiQ3UWA6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042638.
CleanExiMM_GUCY2C.

Interactioni

Subunit structurei

Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36 (By similarity).By similarity

Protein-protein interaction databases

BioGridi200125. 2 interactors.
STRINGi10090.ENSMUSP00000032338.

Structurei

3D structure databases

ProteinModelPortaliQ3UWA6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini489 – 748Protein kinasePROSITE-ProRule annotationAdd BLAST260
Domaini823 – 953Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Domaini

The protein kinase domain is predicted to be catalytically inactive.

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000112833.
HOVERGENiHBG106967.
InParanoidiQ3UWA6.
KOiK12320.
OMAiAYHDAVL.
OrthoDBiEOG091G01F8.
PhylomeDBiQ3UWA6.
TreeFamiTF106338.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UWA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSLLGLAVR LLLFQPALMV FWASQVRQNC RNGSYEISVL MMDNSAYKEP
60 70 80 90 100
MQNLREAVEE GLDIVRKRLR EADLNVTVNA TFIYSDGLIH KSGDCRSSTC
110 120 130 140 150
EGLDLLREIT RDHKMGCALM GPSCTYSTFQ MYLDTELNYP MISAGSYGLS
160 170 180 190 200
CDYKETLTRI LPPARKLMYF LVDFWKVNNA SFKPFSWNSS YVYKNGSEPE
210 220 230 240 250
DCFWYLNALE AGVSYFSEVL NFKDVLRRSE QFQEILTGHN RKSNVIVMCG
260 270 280 290 300
TPESFYDVKG DLQVAEDTVV ILVDLFSNHY FEENTTAPEY MDNVLVLTLP
310 320 330 340 350
SEQSTSNTSV AERFSSGRSD FSLAYLEGTL LFGHMLQTFL ENGENVTGPK
360 370 380 390 400
FARAFRNLTF QGFAGPVTLD DSGDIDNIMS LLYVSLDTRK YKVLMKYDTH
410 420 430 440 450
KNKTIPVAEN PNFIWKNHKL PNDVPGLGPQ ILMIAVFTLT GILVVLLLIA
460 470 480 490 500
LLVLRKYRRD HALRQKKWSH IPSENIFPLE TNETNHISLK IDDDRRRDTI
510 520 530 540 550
QRVRQCKYDK KKVILKDLKH SDGNFSEKQK IDLNKLLQSD YYNLTKFYGT
560 570 580 590 600
VKLDTRIFGV VEYCERGSLR EVLNDTISYP DGTFMDWEFK ISVLNDIAKG
610 620 630 640 650
MSYLHSSKIE VHGRLKSTNC VVDSRMVVKI TDFGCNSILP PKKDLWTAPE
660 670 680 690 700
HLRQATISQK GDVYSFAIIA QEIILRKETF YTLSCRDHNE KIFRVENSYG
710 720 730 740 750
KPFRPDLFLE TADEKELEVY LLVKSCWEED PEKRPDFKKI ESTLAKIFGL
760 770 780 790 800
FHDQKNESYM DTLIRRLQLY SRNLEHLVEE RTQLYKAERD RADHLNFMLL
810 820 830 840 850
PRLVVKSLKE KGIVEPELYE EVTIYFSDIV GFTTICKYST PMEVVDMLND
860 870 880 890 900
IYKSFDQIVD HHDVYKVETI GDAYVVASGL PMRNGNRHAV DISKMALDIL
910 920 930 940 950
SFIGTFELEH LPGLPVWIRI GVHSGPCAAG VVGIKMPRYC LFGDTVNTAS
960 970 980 990 1000
RMESTGLPLR IHMSSSTITI LKRTDCQFLY EVRGETYLKG RGTETTYWLT
1010 1020 1030 1040 1050
GMKDQEYNLP SPPTVENQQR LQTEFSDMIV SALQKRQASG KKSRRPTRVA
1060 1070
SYKKGFLEYM QLNNSDHDST YF
Length:1,072
Mass (Da):123,232
Last modified:October 11, 2005 - v1
Checksum:i7129508166E7790A
GO
Isoform 2 (identifier: Q3UWA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     512-535: Missing.

Note: No experimental confirmation available.
Show »
Length:1,048
Mass (Da):120,452
Checksum:i3F3B0D2056C5B79A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti381L → F in AAH34064 (PubMed:15489334).Curated1
Sequence conflicti969T → A in AAH34064 (PubMed:15489334).Curated1
Sequence conflicti969T → A in AAH99968 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023652512 – 535Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK136496 mRNA. Translation: BAE23010.1.
BC034064 mRNA. Translation: AAH34064.1.
BC099968 mRNA. Translation: AAH99968.1.
CCDSiCCDS20653.1. [Q3UWA6-2]
CCDS51941.1. [Q3UWA6-1]
RefSeqiNP_001120790.1. NM_001127318.1. [Q3UWA6-1]
NP_659504.2. NM_145067.3. [Q3UWA6-2]
UniGeneiMm.222729.

Genome annotation databases

EnsembliENSMUST00000032338; ENSMUSP00000032338; ENSMUSG00000042638. [Q3UWA6-1]
ENSMUST00000078095; ENSMUSP00000077236; ENSMUSG00000042638. [Q3UWA6-2]
GeneIDi14917.
KEGGimmu:14917.
UCSCiuc009emb.2. mouse. [Q3UWA6-2]
uc009emc.2. mouse. [Q3UWA6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK136496 mRNA. Translation: BAE23010.1.
BC034064 mRNA. Translation: AAH34064.1.
BC099968 mRNA. Translation: AAH99968.1.
CCDSiCCDS20653.1. [Q3UWA6-2]
CCDS51941.1. [Q3UWA6-1]
RefSeqiNP_001120790.1. NM_001127318.1. [Q3UWA6-1]
NP_659504.2. NM_145067.3. [Q3UWA6-2]
UniGeneiMm.222729.

3D structure databases

ProteinModelPortaliQ3UWA6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200125. 2 interactors.
STRINGi10090.ENSMUSP00000032338.

PTM databases

iPTMnetiQ3UWA6.
PhosphoSitePlusiQ3UWA6.

Proteomic databases

PaxDbiQ3UWA6.
PeptideAtlasiQ3UWA6.
PRIDEiQ3UWA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032338; ENSMUSP00000032338; ENSMUSG00000042638. [Q3UWA6-1]
ENSMUST00000078095; ENSMUSP00000077236; ENSMUSG00000042638. [Q3UWA6-2]
GeneIDi14917.
KEGGimmu:14917.
UCSCiuc009emb.2. mouse. [Q3UWA6-2]
uc009emc.2. mouse. [Q3UWA6-1]

Organism-specific databases

CTDi2984.
MGIiMGI:106903. Gucy2c.

Phylogenomic databases

eggNOGiKOG1023. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOGENOMiHOG000112833.
HOVERGENiHBG106967.
InParanoidiQ3UWA6.
KOiK12320.
OMAiAYHDAVL.
OrthoDBiEOG091G01F8.
PhylomeDBiQ3UWA6.
TreeFamiTF106338.

Enzyme and pathway databases

BRENDAi4.6.1.2. 3474.

Miscellaneous databases

PROiQ3UWA6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042638.
CleanExiMM_GUCY2C.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR001828. ANF_lig-bd_rcpt.
IPR011009. Kinase-like_dom.
IPR029787. Nucleotide_cyclase.
IPR028082. Peripla_BP_I.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00211. Guanylate_cyc. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
SSF55073. SSF55073. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUC2C_MOUSE
AccessioniPrimary (citable) accession number: Q3UWA6
Secondary accession number(s): Q499D2, Q8JZQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.