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Q3UW12 (CNGA4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclic nucleotide-gated cation channel alpha-4
Alternative name(s):
Cyclic nucleotide-gated channel alpha-4
Short name=CNG channel alpha-4
Short name=CNG-4
Short name=CNG4
Gene names
Name:Cnga4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length575 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Second messenger, cAMP, causes the opening of cation-selective cyclic nucleotide-gated (CNG) channels and depolarization of the neuron (olfactory sensory neurons, OSNs). CNGA4 is the modulatory subunit of this channel which is known to play a central role in the transduction of odorant signals and subsequent adaptation. By accelerating the calcium-mediated negative feedback in olfactory signaling it allows rapid adaptation to odor stimulation and extends its range of odor detection. Ref.3 Ref.4

Enzyme regulation

Calcium-calmodulin exerts its inhibitory effect in cAMP sensitivity by binding to IQ-like motif of CNGA4 and preferably binds to the channel in the closed state. Inhibition by PIP3 of the CNG channel probably occurs via CGNA2 binding By similarity.

Subunit structure

Heterotetramer composed of two subunits of CNGA2, one of CNGA4 and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG) channel of olfactory neurons By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Domain

The C-terminal coiled-coil domain mediates trimerization of CNGA subunits By similarity.

Sequence similarities

Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA4 subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Ontologies

Keywords
   Biological processIon transport
Olfaction
Sensory transduction
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Transmembrane
Transmembrane helix
   LigandcAMP
cAMP-binding
Nucleotide-binding
   Molecular functionIon channel
Ligand-gated ion channel
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphototransduction, visible light

Inferred from Biological aspect of Ancestor. Source: RefGenome

potassium ion transmembrane transport

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of membrane potential

Inferred from Biological aspect of Ancestor. Source: RefGenome

sensory perception of smell

Inferred from mutant phenotype Ref.3Ref.4. Source: MGI

   Cellular_componentintegral component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functioncAMP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cGMP binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

intracellular cAMP activated cation channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

intracellular cGMP activated cation channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

ion channel activity

Traceable author statement Ref.4. Source: MGI

voltage-gated potassium channel activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q3UW12-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q3UW12-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 575575Cyclic nucleotide-gated cation channel alpha-4
PRO_0000317108

Regions

Topological domain1 – 3333Cytoplasmic Potential
Transmembrane34 – 5421Helical; Name=H1; Potential
Topological domain55 – 6511Extracellular Potential
Transmembrane66 – 8621Helical; Name=H2; Potential
Topological domain87 – 11226Cytoplasmic Potential
Transmembrane113 – 13321Helical; Name=H3; Potential
Topological domain134 – 16835Extracellular Potential
Transmembrane169 – 18921Helical; Name=H4; Potential
Topological domain190 – 21829Cytoplasmic Potential
Transmembrane219 – 23921Helical; Name=H5; Potential
Topological domain240 – 2445Extracellular Potential
Transmembrane245 – 26521Helical; Name=H6; Potential
Topological domain266 – 575310Cytoplasmic Potential
Nucleotide binding348 – 471124cNMP
Coiled coil493 – 53644 By similarity
Motif292 – 30211IQ-type By similarity

Natural variations

Alternative sequence1 – 103103Missing in isoform 2.
VSP_030873

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 11, 2005. Version 1.
Checksum: 2E79D58D658D4930

FASTA57565,835
        10         20         30         40         50         60 
MSQDSKVKTT ESTPPAPTKA RKWLPVLDPS GDYYYWWLNT MVFPIMYNLI IVVCRACFPD 

        70         80         90        100        110        120 
LQHSYLVAWF VLDYTSDLLY LLDIGVRFHT GFLEQGILVV DKSMIASRYV RTWSFLLDLA 

       130        140        150        160        170        180 
SLVPTDAAYV QLGPHIPTLR LNRFLRVPRL FEAFDRTETR TAYPNAFRIA KLMIYIFVVI 

       190        200        210        220        230        240 
HWNSCLYFAL SRYLGFGRDA WVYPDPAQPG FERLRRQYLY SFYFSTLILT TVGDTPLPAR 

       250        260        270        280        290        300 
EEEYLFMVGD FLLAVMGFAT IMGSMSSVIY NMNTADAAFY PDHALVKKYM KLQHVNRRLE 

       310        320        330        340        350        360 
RRVIDWYQHL QINKKMTNEV AILQHLPERL RAEVAVSVHL STLSRVQIFQ NCEASLLEEL 

       370        380        390        400        410        420 
VLKLQPQTYS PGEYVCRKGD IGREMYIIRE GQLAVVADDG VTQYAVLGAG LYFGEISIIN 

       430        440        450        460        470        480 
IKGNMSGNRR TANIKSLGYS DLFCLSKEDL REVLSEYPQA QAVMEEKGRE ILLKMNKLDV 

       490        500        510        520        530        540 
NAEAAEIALQ EATESRLKGL DQQLDDLQTK FARLLAELES SALKIAYRIE RLEWQTREWP 

       550        560        570 
MPDDMGEADD EAEPGEGTSK DGEEKAGQEG PSGLE 

« Hide

Isoform 2 [UniParc].

Checksum: 1918AAA0241CD635
Show »

FASTA47253,899

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Epididymis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]"Central role of the CNGA4 channel subunit in Ca2+-calmodulin-dependent odor adaptation."
Munger S.D., Lane A.P., Zhong H., Leinders-Zufall T., Yau K.-W., Zufall F., Reed R.R.
Science 294:2172-2175(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Importance of the CNGA4 channel gene for odor discrimination and adaptation in behaving mice."
Kelliher K.R., Ziesmann J., Munger S.D., Reed R.R., Zufall F.
Proc. Natl. Acad. Sci. U.S.A. 100:4299-4304(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK136723 mRNA. Translation: BAE23107.1.
BC107347 mRNA. Translation: AAI07348.1.
BC107348 mRNA. Translation: AAI07349.1.
RefSeqNP_001028489.1. NM_001033317.3.
XP_006507715.1. XM_006507652.1.
XP_006507717.1. XM_006507654.1.
UniGeneMm.99556.

3D structure databases

ProteinModelPortalQ3UW12.
SMRQ3UW12. Positions 218-260, 286-465.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000033187.

Protein family/group databases

TCDB1.A.1.5.4. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PRIDEQ3UW12.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033187; ENSMUSP00000033187; ENSMUSG00000030897. [Q3UW12-1]
GeneID233649.
KEGGmmu:233649.
UCSCuc009iyc.1. mouse. [Q3UW12-1]

Organism-specific databases

CTD1262.
MGIMGI:2664099. Cnga4.

Phylogenomic databases

eggNOGNOG300831.
GeneTreeENSGT00550000074376.
HOGENOMHOG000007898.
HOVERGENHBG000281.
InParanoidQ3UW12.
KOK04951.
OMAVGHEMYI.
OrthoDBEOG771268.
PhylomeDBQ3UW12.
TreeFamTF319048.

Gene expression databases

BgeeQ3UW12.
CleanExMM_CNGA4.
GenevestigatorQ3UW12.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio381781.
PROQ3UW12.
SOURCESearch...

Entry information

Entry nameCNGA4_MOUSE
AccessionPrimary (citable) accession number: Q3UW12
Secondary accession number(s): Q08EL1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 11, 2005
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot