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Q3UW12

- CNGA4_MOUSE

UniProt

Q3UW12 - CNGA4_MOUSE

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Protein

Cyclic nucleotide-gated cation channel alpha-4

Gene

Cnga4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Second messenger, cAMP, causes the opening of cation-selective cyclic nucleotide-gated (CNG) channels and depolarization of the neuron (olfactory sensory neurons, OSNs). CNGA4 is the modulatory subunit of this channel which is known to play a central role in the transduction of odorant signals and subsequent adaptation. By accelerating the calcium-mediated negative feedback in olfactory signaling it allows rapid adaptation to odor stimulation and extends its range of odor detection.2 Publications

Enzyme regulationi

Calcium-calmodulin exerts its inhibitory effect in cAMP sensitivity by binding to IQ-like motif of CNGA4 and preferably binds to the channel in the closed state. Inhibition by PIP3 of the CNG channel probably occurs via CGNA2 binding (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi348 – 471124cNMPAdd
BLAST

GO - Molecular functioni

  1. cAMP binding Source: UniProtKB-KW
  2. cGMP binding Source: RefGenome
  3. intracellular cAMP activated cation channel activity Source: RefGenome
  4. intracellular cGMP activated cation channel activity Source: RefGenome
  5. ion channel activity Source: MGI
  6. voltage-gated potassium channel activity Source: RefGenome

GO - Biological processi

  1. phototransduction, visible light Source: RefGenome
  2. potassium ion transmembrane transport Source: RefGenome
  3. regulation of membrane potential Source: RefGenome
  4. sensory perception of smell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel

Keywords - Biological processi

Ion transport, Olfaction, Sensory transduction, Transport

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Protein family/group databases

TCDBi1.A.1.5.4. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic nucleotide-gated cation channel alpha-4
Alternative name(s):
Cyclic nucleotide-gated channel alpha-4
Short name:
CNG channel alpha-4
Short name:
CNG-4
Short name:
CNG4
Gene namesi
Name:Cnga4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:2664099. Cnga4.

Subcellular locationi

GO - Cellular componenti

  1. integral component of plasma membrane Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 575575Cyclic nucleotide-gated cation channel alpha-4PRO_0000317108Add
BLAST

Proteomic databases

PRIDEiQ3UW12.

Expressioni

Gene expression databases

BgeeiQ3UW12.
CleanExiMM_CNGA4.
GenevestigatoriQ3UW12.

Interactioni

Subunit structurei

Heterotetramer composed of two subunits of CNGA2, one of CNGA4 and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG) channel of olfactory neurons (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033187.

Structurei

3D structure databases

ProteinModelPortaliQ3UW12.
SMRiQ3UW12. Positions 286-466.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini55 – 6511ExtracellularSequence AnalysisAdd
BLAST
Topological domaini87 – 11226CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini134 – 16835ExtracellularSequence AnalysisAdd
BLAST
Topological domaini190 – 21829CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini240 – 2445ExtracellularSequence Analysis
Topological domaini266 – 575310CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei34 – 5421Helical; Name=H1Sequence AnalysisAdd
BLAST
Transmembranei66 – 8621Helical; Name=H2Sequence AnalysisAdd
BLAST
Transmembranei113 – 13321Helical; Name=H3Sequence AnalysisAdd
BLAST
Transmembranei169 – 18921Helical; Name=H4Sequence AnalysisAdd
BLAST
Transmembranei219 – 23921Helical; Name=H5Sequence AnalysisAdd
BLAST
Transmembranei245 – 26521Helical; Name=H6Sequence AnalysisAdd
BLAST

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili493 – 53644By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi292 – 30211IQ-typeBy similarityAdd
BLAST

Domaini

The C-terminal coiled-coil domain mediates trimerization of CNGA subunits.By similarity

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG300831.
GeneTreeiENSGT00760000118772.
HOGENOMiHOG000007898.
HOVERGENiHBG000281.
InParanoidiQ3UW12.
KOiK04951.
OMAiIIILRTC.
OrthoDBiEOG771268.
PhylomeDBiQ3UW12.
TreeFamiTF319048.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q3UW12-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQDSKVKTT ESTPPAPTKA RKWLPVLDPS GDYYYWWLNT MVFPIMYNLI
60 70 80 90 100
IVVCRACFPD LQHSYLVAWF VLDYTSDLLY LLDIGVRFHT GFLEQGILVV
110 120 130 140 150
DKSMIASRYV RTWSFLLDLA SLVPTDAAYV QLGPHIPTLR LNRFLRVPRL
160 170 180 190 200
FEAFDRTETR TAYPNAFRIA KLMIYIFVVI HWNSCLYFAL SRYLGFGRDA
210 220 230 240 250
WVYPDPAQPG FERLRRQYLY SFYFSTLILT TVGDTPLPAR EEEYLFMVGD
260 270 280 290 300
FLLAVMGFAT IMGSMSSVIY NMNTADAAFY PDHALVKKYM KLQHVNRRLE
310 320 330 340 350
RRVIDWYQHL QINKKMTNEV AILQHLPERL RAEVAVSVHL STLSRVQIFQ
360 370 380 390 400
NCEASLLEEL VLKLQPQTYS PGEYVCRKGD IGREMYIIRE GQLAVVADDG
410 420 430 440 450
VTQYAVLGAG LYFGEISIIN IKGNMSGNRR TANIKSLGYS DLFCLSKEDL
460 470 480 490 500
REVLSEYPQA QAVMEEKGRE ILLKMNKLDV NAEAAEIALQ EATESRLKGL
510 520 530 540 550
DQQLDDLQTK FARLLAELES SALKIAYRIE RLEWQTREWP MPDDMGEADD
560 570
EAEPGEGTSK DGEEKAGQEG PSGLE
Length:575
Mass (Da):65,835
Last modified:October 11, 2005 - v1
Checksum:i2E79D58D658D4930
GO
Isoform 2 (identifier: Q3UW12-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.

Show »
Length:472
Mass (Da):53,899
Checksum:i1918AAA0241CD635
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform 2. 1 PublicationVSP_030873Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK136723 mRNA. Translation: BAE23107.1.
BC107347 mRNA. Translation: AAI07348.1.
BC107348 mRNA. Translation: AAI07349.1.
CCDSiCCDS21650.1. [Q3UW12-1]
RefSeqiNP_001028489.1. NM_001033317.3. [Q3UW12-1]
XP_006507715.1. XM_006507652.1. [Q3UW12-1]
XP_006507717.1. XM_006507654.1. [Q3UW12-2]
UniGeneiMm.99556.

Genome annotation databases

EnsembliENSMUST00000033187; ENSMUSP00000033187; ENSMUSG00000030897. [Q3UW12-1]
GeneIDi233649.
KEGGimmu:233649.
UCSCiuc009iyc.1. mouse. [Q3UW12-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK136723 mRNA. Translation: BAE23107.1 .
BC107347 mRNA. Translation: AAI07348.1 .
BC107348 mRNA. Translation: AAI07349.1 .
CCDSi CCDS21650.1. [Q3UW12-1 ]
RefSeqi NP_001028489.1. NM_001033317.3. [Q3UW12-1 ]
XP_006507715.1. XM_006507652.1. [Q3UW12-1 ]
XP_006507717.1. XM_006507654.1. [Q3UW12-2 ]
UniGenei Mm.99556.

3D structure databases

ProteinModelPortali Q3UW12.
SMRi Q3UW12. Positions 286-466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000033187.

Protein family/group databases

TCDBi 1.A.1.5.4. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PRIDEi Q3UW12.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033187 ; ENSMUSP00000033187 ; ENSMUSG00000030897 . [Q3UW12-1 ]
GeneIDi 233649.
KEGGi mmu:233649.
UCSCi uc009iyc.1. mouse. [Q3UW12-1 ]

Organism-specific databases

CTDi 1262.
MGIi MGI:2664099. Cnga4.

Phylogenomic databases

eggNOGi NOG300831.
GeneTreei ENSGT00760000118772.
HOGENOMi HOG000007898.
HOVERGENi HBG000281.
InParanoidi Q3UW12.
KOi K04951.
OMAi IIILRTC.
OrthoDBi EOG771268.
PhylomeDBi Q3UW12.
TreeFami TF319048.

Miscellaneous databases

NextBioi 381781.
PROi Q3UW12.
SOURCEi Search...

Gene expression databases

Bgeei Q3UW12.
CleanExi MM_CNGA4.
Genevestigatori Q3UW12.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Epididymis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  3. "Central role of the CNGA4 channel subunit in Ca2+-calmodulin-dependent odor adaptation."
    Munger S.D., Lane A.P., Zhong H., Leinders-Zufall T., Yau K.-W., Zufall F., Reed R.R.
    Science 294:2172-2175(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Importance of the CNGA4 channel gene for odor discrimination and adaptation in behaving mice."
    Kelliher K.R., Ziesmann J., Munger S.D., Reed R.R., Zufall F.
    Proc. Natl. Acad. Sci. U.S.A. 100:4299-4304(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCNGA4_MOUSE
AccessioniPrimary (citable) accession number: Q3UW12
Secondary accession number(s): Q08EL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 11, 2005
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3