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Q3UW12

- CNGA4_MOUSE

UniProt

Q3UW12 - CNGA4_MOUSE

Protein

Cyclic nucleotide-gated cation channel alpha-4

Gene

Cnga4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Second messenger, cAMP, causes the opening of cation-selective cyclic nucleotide-gated (CNG) channels and depolarization of the neuron (olfactory sensory neurons, OSNs). CNGA4 is the modulatory subunit of this channel which is known to play a central role in the transduction of odorant signals and subsequent adaptation. By accelerating the calcium-mediated negative feedback in olfactory signaling it allows rapid adaptation to odor stimulation and extends its range of odor detection.2 Publications

    Enzyme regulationi

    Calcium-calmodulin exerts its inhibitory effect in cAMP sensitivity by binding to IQ-like motif of CNGA4 and preferably binds to the channel in the closed state. Inhibition by PIP3 of the CNG channel probably occurs via CGNA2 binding By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi348 – 471124cNMPAdd
    BLAST

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. cGMP binding Source: RefGenome
    3. intracellular cAMP activated cation channel activity Source: RefGenome
    4. intracellular cGMP activated cation channel activity Source: RefGenome
    5. ion channel activity Source: MGI
    6. voltage-gated potassium channel activity Source: RefGenome

    GO - Biological processi

    1. phototransduction, visible light Source: RefGenome
    2. potassium ion transmembrane transport Source: RefGenome
    3. regulation of membrane potential Source: RefGenome
    4. sensory perception of smell Source: MGI

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel

    Keywords - Biological processi

    Ion transport, Olfaction, Sensory transduction, Transport

    Keywords - Ligandi

    cAMP, cAMP-binding, Nucleotide-binding

    Protein family/group databases

    TCDBi1.A.1.5.4. the voltage-gated ion channel (vic) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclic nucleotide-gated cation channel alpha-4
    Alternative name(s):
    Cyclic nucleotide-gated channel alpha-4
    Short name:
    CNG channel alpha-4
    Short name:
    CNG-4
    Short name:
    CNG4
    Gene namesi
    Name:Cnga4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:2664099. Cnga4.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 575575Cyclic nucleotide-gated cation channel alpha-4PRO_0000317108Add
    BLAST

    Proteomic databases

    PRIDEiQ3UW12.

    Expressioni

    Gene expression databases

    BgeeiQ3UW12.
    CleanExiMM_CNGA4.
    GenevestigatoriQ3UW12.

    Interactioni

    Subunit structurei

    Heterotetramer composed of two subunits of CNGA2, one of CNGA4 and one of CNGB1b. The complex forms the cyclic nucleotide-gated (CNG) channel of olfactory neurons By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000033187.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UW12.
    SMRiQ3UW12. Positions 286-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3333CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini55 – 6511ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini87 – 11226CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini134 – 16835ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini190 – 21829CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini240 – 2445ExtracellularSequence Analysis
    Topological domaini266 – 575310CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei34 – 5421Helical; Name=H1Sequence AnalysisAdd
    BLAST
    Transmembranei66 – 8621Helical; Name=H2Sequence AnalysisAdd
    BLAST
    Transmembranei113 – 13321Helical; Name=H3Sequence AnalysisAdd
    BLAST
    Transmembranei169 – 18921Helical; Name=H4Sequence AnalysisAdd
    BLAST
    Transmembranei219 – 23921Helical; Name=H5Sequence AnalysisAdd
    BLAST
    Transmembranei245 – 26521Helical; Name=H6Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili493 – 53644By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi292 – 30211IQ-typeBy similarityAdd
    BLAST

    Domaini

    The C-terminal coiled-coil domain mediates trimerization of CNGA subunits.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG300831.
    GeneTreeiENSGT00550000074376.
    HOGENOMiHOG000007898.
    HOVERGENiHBG000281.
    InParanoidiQ3UW12.
    KOiK04951.
    OMAiIIILRTC.
    OrthoDBiEOG771268.
    PhylomeDBiQ3UW12.
    TreeFamiTF319048.

    Family and domain databases

    Gene3Di2.60.120.10. 1 hit.
    InterProiIPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view]
    SMARTiSM00100. cNMP. 1 hit.
    [Graphical view]
    SUPFAMiSSF51206. SSF51206. 1 hit.
    PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3UW12-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSQDSKVKTT ESTPPAPTKA RKWLPVLDPS GDYYYWWLNT MVFPIMYNLI    50
    IVVCRACFPD LQHSYLVAWF VLDYTSDLLY LLDIGVRFHT GFLEQGILVV 100
    DKSMIASRYV RTWSFLLDLA SLVPTDAAYV QLGPHIPTLR LNRFLRVPRL 150
    FEAFDRTETR TAYPNAFRIA KLMIYIFVVI HWNSCLYFAL SRYLGFGRDA 200
    WVYPDPAQPG FERLRRQYLY SFYFSTLILT TVGDTPLPAR EEEYLFMVGD 250
    FLLAVMGFAT IMGSMSSVIY NMNTADAAFY PDHALVKKYM KLQHVNRRLE 300
    RRVIDWYQHL QINKKMTNEV AILQHLPERL RAEVAVSVHL STLSRVQIFQ 350
    NCEASLLEEL VLKLQPQTYS PGEYVCRKGD IGREMYIIRE GQLAVVADDG 400
    VTQYAVLGAG LYFGEISIIN IKGNMSGNRR TANIKSLGYS DLFCLSKEDL 450
    REVLSEYPQA QAVMEEKGRE ILLKMNKLDV NAEAAEIALQ EATESRLKGL 500
    DQQLDDLQTK FARLLAELES SALKIAYRIE RLEWQTREWP MPDDMGEADD 550
    EAEPGEGTSK DGEEKAGQEG PSGLE 575
    Length:575
    Mass (Da):65,835
    Last modified:October 11, 2005 - v1
    Checksum:i2E79D58D658D4930
    GO
    Isoform 2 (identifier: Q3UW12-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-103: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:472
    Mass (Da):53,899
    Checksum:i1918AAA0241CD635
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 103103Missing in isoform 2. 1 PublicationVSP_030873Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK136723 mRNA. Translation: BAE23107.1.
    BC107347 mRNA. Translation: AAI07348.1.
    BC107348 mRNA. Translation: AAI07349.1.
    CCDSiCCDS21650.1. [Q3UW12-1]
    RefSeqiNP_001028489.1. NM_001033317.3. [Q3UW12-1]
    XP_006507715.1. XM_006507652.1. [Q3UW12-1]
    XP_006507717.1. XM_006507654.1. [Q3UW12-2]
    UniGeneiMm.99556.

    Genome annotation databases

    EnsembliENSMUST00000033187; ENSMUSP00000033187; ENSMUSG00000030897. [Q3UW12-1]
    GeneIDi233649.
    KEGGimmu:233649.
    UCSCiuc009iyc.1. mouse. [Q3UW12-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK136723 mRNA. Translation: BAE23107.1 .
    BC107347 mRNA. Translation: AAI07348.1 .
    BC107348 mRNA. Translation: AAI07349.1 .
    CCDSi CCDS21650.1. [Q3UW12-1 ]
    RefSeqi NP_001028489.1. NM_001033317.3. [Q3UW12-1 ]
    XP_006507715.1. XM_006507652.1. [Q3UW12-1 ]
    XP_006507717.1. XM_006507654.1. [Q3UW12-2 ]
    UniGenei Mm.99556.

    3D structure databases

    ProteinModelPortali Q3UW12.
    SMRi Q3UW12. Positions 286-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000033187.

    Protein family/group databases

    TCDBi 1.A.1.5.4. the voltage-gated ion channel (vic) superfamily.

    Proteomic databases

    PRIDEi Q3UW12.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033187 ; ENSMUSP00000033187 ; ENSMUSG00000030897 . [Q3UW12-1 ]
    GeneIDi 233649.
    KEGGi mmu:233649.
    UCSCi uc009iyc.1. mouse. [Q3UW12-1 ]

    Organism-specific databases

    CTDi 1262.
    MGIi MGI:2664099. Cnga4.

    Phylogenomic databases

    eggNOGi NOG300831.
    GeneTreei ENSGT00550000074376.
    HOGENOMi HOG000007898.
    HOVERGENi HBG000281.
    InParanoidi Q3UW12.
    KOi K04951.
    OMAi IIILRTC.
    OrthoDBi EOG771268.
    PhylomeDBi Q3UW12.
    TreeFami TF319048.

    Miscellaneous databases

    NextBioi 381781.
    PROi Q3UW12.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3UW12.
    CleanExi MM_CNGA4.
    Genevestigatori Q3UW12.

    Family and domain databases

    Gene3Di 2.60.120.10. 1 hit.
    InterProi IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR005821. Ion_trans_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00520. Ion_trans. 1 hit.
    [Graphical view ]
    SMARTi SM00100. cNMP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51206. SSF51206. 1 hit.
    PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
    PS00889. CNMP_BINDING_2. 1 hit.
    PS50042. CNMP_BINDING_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Epididymis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    3. "Central role of the CNGA4 channel subunit in Ca2+-calmodulin-dependent odor adaptation."
      Munger S.D., Lane A.P., Zhong H., Leinders-Zufall T., Yau K.-W., Zufall F., Reed R.R.
      Science 294:2172-2175(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Importance of the CNGA4 channel gene for odor discrimination and adaptation in behaving mice."
      Kelliher K.R., Ziesmann J., Munger S.D., Reed R.R., Zufall F.
      Proc. Natl. Acad. Sci. U.S.A. 100:4299-4304(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCNGA4_MOUSE
    AccessioniPrimary (citable) accession number: Q3UW12
    Secondary accession number(s): Q08EL1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3