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Protein

Metabotropic glutamate receptor 5

Gene

Grm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei64 – 641GlutamateBy similarity
Binding sitei151 – 1511GlutamateBy similarity
Binding sitei222 – 2221GlutamateBy similarity
Binding sitei304 – 3041GlutamateBy similarity
Binding sitei395 – 3951GlutamateBy similarity

GO - Molecular functioni

GO - Biological processi

  • cognition Source: MGI
  • G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  • learning Source: MGI
  • locomotory behavior Source: MGI
  • phospholipase C-activating G-protein coupled glutamate receptor signaling pathway Source: GOC
  • regulation of long-term neuronal synaptic plasticity Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-416476. G alpha (q) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 5
Short name:
mGluR5
Gene namesi
Name:Grm5
Synonyms:Gprc1e, Mglur5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1351342. Grm5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini19 – 579561ExtracellularBy similarityAdd
BLAST
Transmembranei580 – 60223Helical; Name=1By similarityAdd
BLAST
Topological domaini603 – 61210CytoplasmicBy similarity
Transmembranei613 – 63523Helical; Name=2By similarityAdd
BLAST
Topological domaini636 – 6438ExtracellularBy similarity
Transmembranei644 – 66623Helical; Name=3By similarityAdd
BLAST
Topological domaini667 – 69226CytoplasmicBy similarityAdd
BLAST
Transmembranei693 – 71321Helical; Name=4By similarityAdd
BLAST
Topological domaini714 – 73623ExtracellularBy similarityAdd
BLAST
Transmembranei737 – 75822Helical; Name=5By similarityAdd
BLAST
Topological domaini759 – 77113CytoplasmicBy similarityAdd
BLAST
Transmembranei772 – 79423Helical; Name=6By similarityAdd
BLAST
Topological domaini795 – 7973ExtracellularBy similarity
Transmembranei798 – 81922Helical; Name=7By similarityAdd
BLAST
Topological domaini820 – 1203384CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • neuron projection Source: BHF-UCL
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1641352.
GuidetoPHARMACOLOGYi293.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 12031185Metabotropic glutamate receptor 5PRO_0000306853Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi57 ↔ 99By similarity
Glycosylationi88 – 881N-linked (GlcNAc...)Sequence analysis
Glycosylationi209 – 2091N-linked (GlcNAc...)Sequence analysis
Disulfide bondi240 ↔ 529By similarity
Disulfide bondi275 ↔ 277By similarity
Disulfide bondi364 ↔ 380By similarity
Glycosylationi377 – 3771N-linked (GlcNAc...)Sequence analysis
Glycosylationi381 – 3811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi418 ↔ 425By similarity
Glycosylationi444 – 4441N-linked (GlcNAc...)Sequence analysis
Disulfide bondi510 ↔ 530By similarity
Disulfide bondi514 ↔ 533By similarity
Disulfide bondi536 ↔ 548By similarity
Disulfide bondi551 ↔ 564By similarity
Disulfide bondi643 ↔ 732By similarity
Glycosylationi733 – 7331N-linked (GlcNAc...)Sequence analysis
Modified residuei860 – 8601PhosphoserineBy similarity
Modified residuei1014 – 10141PhosphoserineCombined sources
Modified residuei1016 – 10161PhosphoserineCombined sources

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ3UVX5.
PaxDbiQ3UVX5.
PRIDEiQ3UVX5.

PTM databases

iPTMnetiQ3UVX5.
PhosphoSiteiQ3UVX5.

Expressioni

Gene expression databases

BgeeiQ3UVX5.
CleanExiMM_GRM5.
ExpressionAtlasiQ3UVX5. baseline and differential.
GenevisibleiQ3UVX5. MM.

Interactioni

Subunit structurei

The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3 (By similarity). Interacts with SIAH1 and GRASP. Interacts with NCDN (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Homer1Q9Z2142EBI-8795045,EBI-2338940From a different organism.
Homer1Q9Z214-13EBI-8795045,EBI-4410552From a different organism.
Homer1Q9Z214-32EBI-8795045,EBI-2339003From a different organism.
PIN1Q135262EBI-8795045,EBI-714158From a different organism.

Protein-protein interaction databases

BioGridi223809. 7 interactions.
IntActiQ3UVX5. 4 interactions.
STRINGi10090.ENSMUSP00000129181.

Structurei

3D structure databases

ProteinModelPortaliQ3UVX5.
SMRiQ3UVX5. Positions 26-494, 566-832.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1743Glutamate bindingBy similarity
Regioni804 – 8085Allosteric effector bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi121 – 1244Poly-Glu
Compositional biasi1046 – 10494Poly-Ser
Compositional biasi1199 – 12024Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218636.
HOVERGENiHBG107965.
InParanoidiQ3UVX5.
KOiK04604.
OMAiLRPETNH.
PhylomeDBiQ3UVX5.
TreeFamiTF313240.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000202. GPCR_3_mtglu_rcpt_5.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01055. MTABOTROPC5R.
PR00593. MTABOTROPICR.
SMARTiSM01229. GluR_Homer-bdg. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UVX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLLLILSVL LLKEDVRGSA QSSERRVVAH MPGDIIIGAL FSVHHQPTVD
60 70 80 90 100
KVHERKCGAV REQYGIQRVE AMLHTLERIN SDPTLLPNIT LGCEIRDSCW
110 120 130 140 150
HSAVALEQSI EFIRDSLISS EEEEGLVRCV DGSSSFRSKK PIVGVIGPGS
160 170 180 190 200
SSVAIQVQNL LQLFNIPQIA YSATSMDLSD KTLFKYFMRV VPSDAQQARA
210 220 230 240 250
MVDIVKRYNW TYVSAVHTEG NYGESGMEAF KDMSAKEGIC IAHSYKIYSN
260 270 280 290 300
AGEQSFDKLL KKLRSHLPKA RVVACFCEGM TVRGLLMAMR RLGLAGEFLL
310 320 330 340 350
LGSDGWADRY DVTDGYQREA VGGITIKLQS PDVKWFDDYY LKLRPETNLR
360 370 380 390 400
NPWFQEFWQH RFQCRLEGFA QENSKYNKTC NSSLTLRTHH VQDSKMGFVI
410 420 430 440 450
NAIYSMAYGL HNMQMSLCPG YAGLCDAMKP IDGRKLLDSL MKTNFTGVSG
460 470 480 490 500
DMILFDENGD SPGRYEIMNF KEMGKDYFDY INVGSWDNGE LKMDDDEVWS
510 520 530 540 550
KKNNIIRSVC SEPCEKGQIK VIRKGEVSCC WTCTPCKENE YVFDEYTCKA
560 570 580 590 600
CQLGSWPTDD LTGCDLIPVQ YLRWGDPEPI AAVVFACLGL LATLFVTVIF
610 620 630 640 650
IIYRDTPVVK SSSRELCYII LAGICLGYLC TFCLIAKPKQ IYCYLQRIGI
660 670 680 690 700
GLSPAMSYSA LVTKTNRIAR ILAGSKKKIC TKKPRFMSAC AQLVIAFILI
710 720 730 740 750
CIQLGIIVAL FIMEPPDIMH DYPSIREVYL ICNTTNLGVV TPLGYNGLLI
760 770 780 790 800
LSCTFYAFKT RNVPANFNEA KYIAFTMYTT CIIWLAFVPI YFGSNYKIIT
810 820 830 840 850
MCFSVSLSAT VALGCMFVPK VYIILAKPER NVRSAFTTST VVRMHVGDGK
860 870 880 890 900
SSSAASRSSS LVNLWKRRGS SGETLRYKDR RLAQHKSEIE CFTPKGSMGN
910 920 930 940 950
GGRATMSSSN GKSVTWAQNE KSTRGQHLWQ RLSVHINKKE NPNQTAVIKP
960 970 980 990 1000
FPKSTESRGQ GAGAGGGSGP GAAGAGSAGC TATGGPEPPD AGPKALYDVA
1010 1020 1030 1040 1050
EAEERFPAAA RPRSPSPIST LSHLAGSAGR TDDDAPSLHS ETAARSSSSQ
1060 1070 1080 1090 1100
GSLMEQISSV VTRFTANITE LNSMMLSTAA APGPPGTPIC SSYLIPKEIQ
1110 1120 1130 1140 1150
LPTTMTTFAE IQPLPAIEVT GGAQPATGPS PAQETPAGAE AAPGKPDLEE
1160 1170 1180 1190 1200
LVALTPPSPF RDSVDSGSTT PNSPVSESAL CIPSSPKYDT LIIRDYTQSS

SSL
Length:1,203
Mass (Da):131,865
Last modified:October 2, 2007 - v2
Checksum:i2A09D7EC272ED23C
GO
Isoform 2 (identifier: Q3UVX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-303: S → R
     304-1203: Missing.

Show »
Length:303
Mass (Da):33,648
Checksum:iFFE2A5005A44920C
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei303 – 3031S → R in isoform 2. 1 PublicationVSP_028519
Alternative sequencei304 – 1203900Missing in isoform 2. 1 PublicationVSP_028520Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK136840 mRNA. Translation: BAE23144.1.
AC113033 Genomic DNA. No translation available.
AC113115 Genomic DNA. No translation available.
AC116820 Genomic DNA. No translation available.
AC149088 Genomic DNA. No translation available.
AC163218 Genomic DNA. No translation available.
CCDSiCCDS52305.1. [Q3UVX5-1]
RefSeqiNP_001137306.1. NM_001143834.1. [Q3UVX5-1]
XP_011239945.1. XM_011241643.1. [Q3UVX5-1]
UniGeneiMm.235018.

Genome annotation databases

EnsembliENSMUST00000155358; ENSMUSP00000114927; ENSMUSG00000049583. [Q3UVX5-1]
GeneIDi108071.
KEGGimmu:108071.
UCSCiuc009ifp.2. mouse. [Q3UVX5-2]
uc009ifs.2. mouse. [Q3UVX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK136840 mRNA. Translation: BAE23144.1.
AC113033 Genomic DNA. No translation available.
AC113115 Genomic DNA. No translation available.
AC116820 Genomic DNA. No translation available.
AC149088 Genomic DNA. No translation available.
AC163218 Genomic DNA. No translation available.
CCDSiCCDS52305.1. [Q3UVX5-1]
RefSeqiNP_001137306.1. NM_001143834.1. [Q3UVX5-1]
XP_011239945.1. XM_011241643.1. [Q3UVX5-1]
UniGeneiMm.235018.

3D structure databases

ProteinModelPortaliQ3UVX5.
SMRiQ3UVX5. Positions 26-494, 566-832.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223809. 7 interactions.
IntActiQ3UVX5. 4 interactions.
STRINGi10090.ENSMUSP00000129181.

Chemistry

ChEMBLiCHEMBL1641352.
GuidetoPHARMACOLOGYi293.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ3UVX5.
PhosphoSiteiQ3UVX5.

Proteomic databases

MaxQBiQ3UVX5.
PaxDbiQ3UVX5.
PRIDEiQ3UVX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000155358; ENSMUSP00000114927; ENSMUSG00000049583. [Q3UVX5-1]
GeneIDi108071.
KEGGimmu:108071.
UCSCiuc009ifp.2. mouse. [Q3UVX5-2]
uc009ifs.2. mouse. [Q3UVX5-1]

Organism-specific databases

CTDi2915.
MGIiMGI:1351342. Grm5.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218636.
HOVERGENiHBG107965.
InParanoidiQ3UVX5.
KOiK04604.
OMAiLRPETNH.
PhylomeDBiQ3UVX5.
TreeFamiTF313240.

Enzyme and pathway databases

ReactomeiR-MMU-416476. G alpha (q) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).

Miscellaneous databases

NextBioi359988.
PROiQ3UVX5.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UVX5.
CleanExiMM_GRM5.
ExpressionAtlasiQ3UVX5. baseline and differential.
GenevisibleiQ3UVX5. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000202. GPCR_3_mtglu_rcpt_5.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01055. MTABOTROPC5R.
PR00593. MTABOTROPICR.
SMARTiSM01229. GluR_Homer-bdg. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Diencephalon.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1014 AND SER-1016, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiGRM5_MOUSE
AccessioniPrimary (citable) accession number: Q3UVX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: May 11, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.