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Protein

Metabotropic glutamate receptor 5

Gene

Grm5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei64GlutamateBy similarity1
Binding sitei151GlutamateBy similarity1
Binding sitei222GlutamateBy similarity1
Binding sitei304GlutamateBy similarity1
Binding sitei395GlutamateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cognition Source: MGI
  • G-protein coupled glutamate receptor signaling pathway Source: UniProtKB
  • learning Source: MGI
  • locomotory behavior Source: MGI
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: GO_Central
  • regulation of long-term neuronal synaptic plasticity Source: MGI
  • regulation of synaptic transmission, glutamatergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-416476. G alpha (q) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).
R-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Metabotropic glutamate receptor 5
Short name:
mGluR5
Gene namesi
Name:Grm5
Synonyms:Gprc1e, Mglur5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1351342. Grm5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 579ExtracellularBy similarityAdd BLAST561
Transmembranei580 – 602Helical; Name=1By similarityAdd BLAST23
Topological domaini603 – 612CytoplasmicBy similarity10
Transmembranei613 – 635Helical; Name=2By similarityAdd BLAST23
Topological domaini636 – 643ExtracellularBy similarity8
Transmembranei644 – 666Helical; Name=3By similarityAdd BLAST23
Topological domaini667 – 692CytoplasmicBy similarityAdd BLAST26
Transmembranei693 – 713Helical; Name=4By similarityAdd BLAST21
Topological domaini714 – 736ExtracellularBy similarityAdd BLAST23
Transmembranei737 – 758Helical; Name=5By similarityAdd BLAST22
Topological domaini759 – 771CytoplasmicBy similarityAdd BLAST13
Transmembranei772 – 794Helical; Name=6By similarityAdd BLAST23
Topological domaini795 – 797ExtracellularBy similarity3
Transmembranei798 – 819Helical; Name=7By similarityAdd BLAST22
Topological domaini820 – 1203CytoplasmicBy similarityAdd BLAST384

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • integral component of plasma membrane Source: UniProtKB
  • neuron projection Source: BHF-UCL
  • plasma membrane Source: MGI
  • postsynaptic density Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1641352.
GuidetoPHARMACOLOGYi293.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000030685319 – 1203Metabotropic glutamate receptor 5Add BLAST1185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 99By similarity
Glycosylationi88N-linked (GlcNAc...)Sequence analysis1
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi240 ↔ 529By similarity
Disulfide bondi275 ↔ 277By similarity
Disulfide bondi364 ↔ 380By similarity
Glycosylationi377N-linked (GlcNAc...)Sequence analysis1
Glycosylationi381N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi418 ↔ 425By similarity
Glycosylationi444N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi510 ↔ 530By similarity
Disulfide bondi514 ↔ 533By similarity
Disulfide bondi536 ↔ 548By similarity
Disulfide bondi551 ↔ 564By similarity
Disulfide bondi643 ↔ 732By similarity
Glycosylationi733N-linked (GlcNAc...)Sequence analysis1
Modified residuei860PhosphoserineBy similarity1
Modified residuei868Omega-N-methylarginineCombined sources1
Modified residuei924Omega-N-methylarginineCombined sources1
Modified residuei1014PhosphoserineCombined sources1
Modified residuei1016PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ3UVX5.
PeptideAtlasiQ3UVX5.
PRIDEiQ3UVX5.

PTM databases

iPTMnetiQ3UVX5.
PhosphoSitePlusiQ3UVX5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000049583.
CleanExiMM_GRM5.
ExpressionAtlasiQ3UVX5. baseline and differential.
GenevisibleiQ3UVX5. MM.

Interactioni

Subunit structurei

The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3 (By similarity). Interacts with SIAH1 and GRASP. Interacts with NCDN (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Homer1Q9Z2142EBI-8795045,EBI-2338940From a different organism.
Homer1Q9Z214-13EBI-8795045,EBI-4410552From a different organism.
Homer1Q9Z214-32EBI-8795045,EBI-2339003From a different organism.
PIN1Q135262EBI-8795045,EBI-714158From a different organism.

Protein-protein interaction databases

BioGridi223809. 7 interactors.
IntActiQ3UVX5. 4 interactors.
STRINGi10090.ENSMUSP00000129181.

Chemistry databases

BindingDBiQ3UVX5.

Structurei

3D structure databases

ProteinModelPortaliQ3UVX5.
SMRiQ3UVX5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 174Glutamate bindingBy similarity3
Regioni804 – 808Allosteric effector bindingBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi121 – 124Poly-Glu4
Compositional biasi1046 – 1049Poly-Ser4
Compositional biasi1199 – 1202Poly-Ser4

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218636.
HOVERGENiHBG107965.
InParanoidiQ3UVX5.
KOiK04604.
OMAiLRPETNH.
OrthoDBiEOG091G177R.
PhylomeDBiQ3UVX5.
TreeFamiTF313240.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000202. GPCR_3_mtglu_rcpt_5.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01055. MTABOTROPC5R.
PR00593. MTABOTROPICR.
SMARTiSM01229. GluR_Homer-bdg. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UVX5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLLLILSVL LLKEDVRGSA QSSERRVVAH MPGDIIIGAL FSVHHQPTVD
60 70 80 90 100
KVHERKCGAV REQYGIQRVE AMLHTLERIN SDPTLLPNIT LGCEIRDSCW
110 120 130 140 150
HSAVALEQSI EFIRDSLISS EEEEGLVRCV DGSSSFRSKK PIVGVIGPGS
160 170 180 190 200
SSVAIQVQNL LQLFNIPQIA YSATSMDLSD KTLFKYFMRV VPSDAQQARA
210 220 230 240 250
MVDIVKRYNW TYVSAVHTEG NYGESGMEAF KDMSAKEGIC IAHSYKIYSN
260 270 280 290 300
AGEQSFDKLL KKLRSHLPKA RVVACFCEGM TVRGLLMAMR RLGLAGEFLL
310 320 330 340 350
LGSDGWADRY DVTDGYQREA VGGITIKLQS PDVKWFDDYY LKLRPETNLR
360 370 380 390 400
NPWFQEFWQH RFQCRLEGFA QENSKYNKTC NSSLTLRTHH VQDSKMGFVI
410 420 430 440 450
NAIYSMAYGL HNMQMSLCPG YAGLCDAMKP IDGRKLLDSL MKTNFTGVSG
460 470 480 490 500
DMILFDENGD SPGRYEIMNF KEMGKDYFDY INVGSWDNGE LKMDDDEVWS
510 520 530 540 550
KKNNIIRSVC SEPCEKGQIK VIRKGEVSCC WTCTPCKENE YVFDEYTCKA
560 570 580 590 600
CQLGSWPTDD LTGCDLIPVQ YLRWGDPEPI AAVVFACLGL LATLFVTVIF
610 620 630 640 650
IIYRDTPVVK SSSRELCYII LAGICLGYLC TFCLIAKPKQ IYCYLQRIGI
660 670 680 690 700
GLSPAMSYSA LVTKTNRIAR ILAGSKKKIC TKKPRFMSAC AQLVIAFILI
710 720 730 740 750
CIQLGIIVAL FIMEPPDIMH DYPSIREVYL ICNTTNLGVV TPLGYNGLLI
760 770 780 790 800
LSCTFYAFKT RNVPANFNEA KYIAFTMYTT CIIWLAFVPI YFGSNYKIIT
810 820 830 840 850
MCFSVSLSAT VALGCMFVPK VYIILAKPER NVRSAFTTST VVRMHVGDGK
860 870 880 890 900
SSSAASRSSS LVNLWKRRGS SGETLRYKDR RLAQHKSEIE CFTPKGSMGN
910 920 930 940 950
GGRATMSSSN GKSVTWAQNE KSTRGQHLWQ RLSVHINKKE NPNQTAVIKP
960 970 980 990 1000
FPKSTESRGQ GAGAGGGSGP GAAGAGSAGC TATGGPEPPD AGPKALYDVA
1010 1020 1030 1040 1050
EAEERFPAAA RPRSPSPIST LSHLAGSAGR TDDDAPSLHS ETAARSSSSQ
1060 1070 1080 1090 1100
GSLMEQISSV VTRFTANITE LNSMMLSTAA APGPPGTPIC SSYLIPKEIQ
1110 1120 1130 1140 1150
LPTTMTTFAE IQPLPAIEVT GGAQPATGPS PAQETPAGAE AAPGKPDLEE
1160 1170 1180 1190 1200
LVALTPPSPF RDSVDSGSTT PNSPVSESAL CIPSSPKYDT LIIRDYTQSS

SSL
Length:1,203
Mass (Da):131,865
Last modified:October 2, 2007 - v2
Checksum:i2A09D7EC272ED23C
GO
Isoform 2 (identifier: Q3UVX5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     303-303: S → R
     304-1203: Missing.

Show »
Length:303
Mass (Da):33,648
Checksum:iFFE2A5005A44920C
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_028519303S → R in isoform 2. 1 Publication1
Alternative sequenceiVSP_028520304 – 1203Missing in isoform 2. 1 PublicationAdd BLAST900

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK136840 mRNA. Translation: BAE23144.1.
AC113033 Genomic DNA. No translation available.
AC113115 Genomic DNA. No translation available.
AC116820 Genomic DNA. No translation available.
AC149088 Genomic DNA. No translation available.
AC163218 Genomic DNA. No translation available.
CCDSiCCDS52305.1. [Q3UVX5-1]
RefSeqiNP_001137306.1. NM_001143834.1. [Q3UVX5-1]
XP_011239945.1. XM_011241643.2. [Q3UVX5-1]
UniGeneiMm.235018.

Genome annotation databases

EnsembliENSMUST00000155358; ENSMUSP00000114927; ENSMUSG00000049583. [Q3UVX5-1]
GeneIDi108071.
KEGGimmu:108071.
UCSCiuc009ifp.2. mouse. [Q3UVX5-2]
uc009ifs.2. mouse. [Q3UVX5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK136840 mRNA. Translation: BAE23144.1.
AC113033 Genomic DNA. No translation available.
AC113115 Genomic DNA. No translation available.
AC116820 Genomic DNA. No translation available.
AC149088 Genomic DNA. No translation available.
AC163218 Genomic DNA. No translation available.
CCDSiCCDS52305.1. [Q3UVX5-1]
RefSeqiNP_001137306.1. NM_001143834.1. [Q3UVX5-1]
XP_011239945.1. XM_011241643.2. [Q3UVX5-1]
UniGeneiMm.235018.

3D structure databases

ProteinModelPortaliQ3UVX5.
SMRiQ3UVX5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi223809. 7 interactors.
IntActiQ3UVX5. 4 interactors.
STRINGi10090.ENSMUSP00000129181.

Chemistry databases

BindingDBiQ3UVX5.
ChEMBLiCHEMBL1641352.
GuidetoPHARMACOLOGYi293.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ3UVX5.
PhosphoSitePlusiQ3UVX5.

Proteomic databases

PaxDbiQ3UVX5.
PeptideAtlasiQ3UVX5.
PRIDEiQ3UVX5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000155358; ENSMUSP00000114927; ENSMUSG00000049583. [Q3UVX5-1]
GeneIDi108071.
KEGGimmu:108071.
UCSCiuc009ifp.2. mouse. [Q3UVX5-2]
uc009ifs.2. mouse. [Q3UVX5-1]

Organism-specific databases

CTDi2915.
MGIiMGI:1351342. Grm5.

Phylogenomic databases

eggNOGiKOG1056. Eukaryota.
ENOG410XR6W. LUCA.
GeneTreeiENSGT00760000118884.
HOGENOMiHOG000218636.
HOVERGENiHBG107965.
InParanoidiQ3UVX5.
KOiK04604.
OMAiLRPETNH.
OrthoDBiEOG091G177R.
PhylomeDBiQ3UVX5.
TreeFamiTF313240.

Enzyme and pathway databases

ReactomeiR-MMU-416476. G alpha (q) signalling events.
R-MMU-420499. Class C/3 (Metabotropic glutamate/pheromone receptors).
R-MMU-6794361. Interactions of neurexins and neuroligins at synapses.

Miscellaneous databases

PROiQ3UVX5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000049583.
CleanExiMM_GRM5.
ExpressionAtlasiQ3UVX5. baseline and differential.
GenevisibleiQ3UVX5. MM.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR000202. GPCR_3_mtglu_rcpt_5.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSiPR00248. GPCRMGR.
PR01055. MTABOTROPC5R.
PR00593. MTABOTROPICR.
SMARTiSM01229. GluR_Homer-bdg. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.
PROSITEiPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRM5_MOUSE
AccessioniPrimary (citable) accession number: Q3UVX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: November 2, 2016
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.