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Protein

Integrin beta-2-like protein

Gene

Itgb2l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

During inflammatory stimulation, plays a role in retaining Cxcl13-expressing cells at the site of the inflammatory response.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Integrin

Keywords - Biological processi

Inflammatory response

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin beta-2-like protein
Alternative name(s):
Protein pactolus
Gene namesi
Name:Itgb2lImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1277979. Itgb2l.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 671ExtracellularSequence analysisAdd BLAST649
Transmembranei672 – 692HelicalSequence analysisAdd BLAST21
Topological domaini693 – 738CytoplasmicSequence analysisAdd BLAST46

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
  • secretory granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display normal neutrophil maturation and function including appropriate migration into sites of inflammation and response to bacterial infection. Following inflammatory stimulus in the peritoneal cavity, they display decreased levels of Cxcl13 due to the migration of resident Cxcl13-expressing macrophages from the peritoneal cavity during the inflammatory response.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000027812423 – 738Integrin beta-2-like proteinAdd BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 419By similarity
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi33 ↔ 43By similarity
Disulfide bondi36 ↔ 73By similarity
Disulfide bondi46 ↔ 62By similarity
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
Glycosylationi102N-linked (GlcNAc...)Sequence analysis1
Glycosylationi173N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi218 ↔ 258By similarity
Glycosylationi226N-linked (GlcNAc...)Sequence analysis1
Glycosylationi252N-linked (GlcNAc...)Sequence analysis1
Glycosylationi342N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi358 ↔ 372By similarity
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Glycosylationi386N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi417 ↔ 421By similarity
Disulfide bondi439 ↔ 478By similarity
Disulfide bondi444 ↔ 453By similarity
Disulfide bondi455 ↔ 469By similarity
Glycosylationi473N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi484 ↔ 489By similarity
Disulfide bondi486 ↔ 521By similarity
Disulfide bondi491 ↔ 506By similarity
Disulfide bondi508 ↔ 513By similarity
Disulfide bondi529 ↔ 534By similarity
Disulfide bondi531 ↔ 562By similarity
Disulfide bondi536 ↔ 545By similarity
Disulfide bondi547 ↔ 554By similarity
Disulfide bondi568 ↔ 573By similarity
Disulfide bondi575 ↔ 584By similarity
Disulfide bondi594 ↔ 603By similarity
Disulfide bondi600 ↔ 664By similarity
Glycosylationi627N-linked (GlcNAc...)Sequence analysis1
Glycosylationi669N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ3UV74.
PRIDEiQ3UV74.

PTM databases

iPTMnetiQ3UV74.
PhosphoSitePlusiQ3UV74.

Expressioni

Tissue specificityi

Expressed predominantly in maturing and mature neutrophils.1 Publication

Gene expression databases

BgeeiENSMUSG00000000157.
CleanExiMM_ITGB2L.
ExpressionAtlasiQ3UV74. baseline and differential.
GenevisibleiQ3UV74. MM.

Interactioni

Subunit structurei

Monomer and homodimer (Probable). Unlike integrin beta chains, no alpha chain partner has yet been found.Curated2 Publications

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000161.

Structurei

Secondary structure

1738
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi127 – 134Combined sources8
Helixi137 – 139Combined sources3
Turni140 – 142Combined sources3
Helixi143 – 160Combined sources18
Beta strandi164 – 174Combined sources11
Beta strandi176 – 184Combined sources9
Helixi186 – 194Combined sources9
Beta strandi202 – 206Combined sources5
Helixi208 – 217Combined sources10
Helixi219 – 222Combined sources4
Beta strandi226 – 234Combined sources9
Helixi244 – 248Combined sources5
Beta strandi260 – 265Combined sources6
Helixi266 – 269Combined sources4
Helixi276 – 286Combined sources11
Beta strandi289 – 295Combined sources7
Helixi296 – 308Combined sources13
Beta strandi313 – 319Combined sources7
Helixi322 – 333Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUENMR-A124-335[»]
ProteinModelPortaliQ3UV74.
SMRiQ3UV74.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3UV74.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 74PSISequence analysisAdd BLAST51
Domaini126 – 329VWFASequence analysisAdd BLAST204

Sequence similaritiesi

Belongs to the integrin beta chain family.Sequence analysis
Contains 1 PSI domain.Sequence analysis
Contains 1 VWFA domain.Sequence analysis

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
GeneTreeiENSGT00760000119064.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiQ3UV74.
KOiK06464.
PhylomeDBiQ3UV74.
TreeFamiTF105392.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR033760. Integin_beta_N.
IPR015812. Integrin_bsu.
IPR012896. Integrin_bsu_tail.
IPR002369. Integrin_bsu_VWA.
IPR032695. Integrin_dom.
IPR016201. PSI.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PfamiPF00362. Integrin_beta. 2 hits.
PF17205. PSI_integrin. 1 hit.
[Graphical view]
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3UV74-1) [UniParc]FASTAAdd to basket
Also known as: A1 Publication, Membrane-bound1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLGQCTLLPV LAGLLSLESA LSQLCTKDNV STCQDCIRSG PSCAWCQKLN
60 70 80 90 100
FTGRGEPDSV RCDTPEQLLL KGCTSEYLVD PKSLAESQED KERDQRQLSP
110 120 130 140 150
RNVTVFLRPG QAATFKVDFQ RTQDNSVDLY FLMGLSGSAQ GHLSNVQTLG
160 170 180 190 200
SDLLKALNEI SRSGRIGFGS IVNMTFQHIL KLTADSSQFQ RELRKQLVSG
210 220 230 240 250
KLATPKGQLD AVVQVAICLG EIGWRNGTRF LVLVTDNDFH LAKDKTLGTR
260 270 280 290 300
QNTSDGRCHL DDGMYRSRGE PDYQSVVQLA SKLAENNIQP IFVVPSRMVK
310 320 330 340 350
TYEKLTTFIP KLTIGELSDD SSNVAQLIRN AYSKLSSIVV LNHSTIPSIL
360 370 380 390 400
KVTYDSYCSN GTSNPGKPSG DCSGVQINDQ VTFQVNITAS ECFREQFFFI
410 420 430 440 450
QALGFMDSVT VRVLPLCECQ CQEQSQHHSL CGGKGAMECG ICRCNSGYAG
460 470 480 490 500
KNCECQTQGP SSQDLEGSCR KDNSSIMCSG LGDCICGQCE CHTSDIPNKE
510 520 530 540 550
IYGQYCECNN VNCERYDGQV CGGPERGHCS CGRCFCRYGF VGSACQCRMS
560 570 580 590 600
TSGCLNNRMV ECSGHGRCYC NRCLCDPGYQ PPLCEKRPGY FHRCSEYYSC
610 620 630 640 650
ARCLKDNSAI KCRECWNLLF SNTPFSNKTC MTERDSEGCW TTYTLYQPDQ
660 670 680 690 700
SDINSIYIKE SLVCAEISNT TILLGVIVGV LLAVIFLLVY CMVYLKGTQK
710 720 730
AAKLPRKGGA QSTLAQQPHF QEPHHVEPVW NQERQGTQ
Length:738
Mass (Da):81,547
Last modified:October 11, 2005 - v1
Checksum:iCCAA3138FEFF0D5A
GO
Isoform 21 Publication (identifier: Q3UV74-2) [UniParc]FASTAAdd to basket
Also known as: B1 Publication, Truncated1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     525-540: ERGHCSCGRCFCRYGF → ASWAQPASAGCPLQAV
     541-738: Missing.

Show »
Length:540
Mass (Da):58,892
Checksum:i73ADCB17A9533ED9
GO
Isoform 31 Publication (identifier: Q3UV74-3) [UniParc]FASTAAdd to basket
Also known as: C1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     432-490: GGKGAMECGI...LGDCICGQCE → LQEDESWQPR...GVQWPWSMLL
     491-738: Missing.

Show »
Length:490
Mass (Da):54,554
Checksum:iD6869101C85F9F75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti509N → D in AAC25502 (PubMed:9535848).Curated1
Sequence conflicti509N → D in AAI13143 (PubMed:15489334).Curated1
Sequence conflicti710A → V in AAC25502 (PubMed:9535848).Curated1

Polymorphismi

Strain C57BL/6 preferentially expresses isoform 1 while strains BALB/c and C3H/HeJ preferentially express isoform 2. This is due to a single nucleotide difference at the second splice acceptor site in exon 13 which results in production of isoform 2 when this splice site is used in strains BALB/c and C3H/HeJ.1 Publication

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti539G → S in strain: BALB/c and C3H/HeJ. 1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_052319432 – 490GGKGA…CGQCE → LQEDESWQPRLFSRKRPLLL WPMLLSLQLRGLSLPVPDVH FRLSEQQDGGVQWPWSMLL in isoform 3. 1 PublicationAdd BLAST59
Alternative sequenceiVSP_052320491 – 738Missing in isoform 3. 1 PublicationAdd BLAST248
Alternative sequenceiVSP_052321525 – 540ERGHC…CRYGF → ASWAQPASAGCPLQAV in isoform 2. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_052322541 – 738Missing in isoform 2. 1 PublicationAdd BLAST198

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051367 mRNA. Translation: AAC25502.1.
AK137534 mRNA. Translation: BAE23399.1.
BC113142 mRNA. Translation: AAI13143.1.
CCDSiCCDS28358.1. [Q3UV74-1]
RefSeqiNP_032431.2. NM_008405.3. [Q3UV74-1]
UniGeneiMm.12872.

Genome annotation databases

EnsembliENSMUST00000000161; ENSMUSP00000000161; ENSMUSG00000000157. [Q3UV74-1]
ENSMUST00000131567; ENSMUSP00000114497; ENSMUSG00000000157. [Q3UV74-2]
GeneIDi16415.
KEGGimmu:16415.
UCSCiuc008adb.1. mouse. [Q3UV74-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051367 mRNA. Translation: AAC25502.1.
AK137534 mRNA. Translation: BAE23399.1.
BC113142 mRNA. Translation: AAI13143.1.
CCDSiCCDS28358.1. [Q3UV74-1]
RefSeqiNP_032431.2. NM_008405.3. [Q3UV74-1]
UniGeneiMm.12872.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUENMR-A124-335[»]
ProteinModelPortaliQ3UV74.
SMRiQ3UV74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000000161.

PTM databases

iPTMnetiQ3UV74.
PhosphoSitePlusiQ3UV74.

Proteomic databases

PaxDbiQ3UV74.
PRIDEiQ3UV74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000161; ENSMUSP00000000161; ENSMUSG00000000157. [Q3UV74-1]
ENSMUST00000131567; ENSMUSP00000114497; ENSMUSG00000000157. [Q3UV74-2]
GeneIDi16415.
KEGGimmu:16415.
UCSCiuc008adb.1. mouse. [Q3UV74-1]

Organism-specific databases

CTDi16415.
MGIiMGI:1277979. Itgb2l.

Phylogenomic databases

eggNOGiKOG1226. Eukaryota.
ENOG410XP60. LUCA.
GeneTreeiENSGT00760000119064.
HOGENOMiHOG000252936.
HOVERGENiHBG006190.
InParanoidiQ3UV74.
KOiK06464.
PhylomeDBiQ3UV74.
TreeFamiTF105392.

Enzyme and pathway databases

ReactomeiR-MMU-166016. Toll Like Receptor 4 (TLR4) Cascade.
R-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202733. Cell surface interactions at the vascular wall.
R-MMU-216083. Integrin cell surface interactions.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiQ3UV74.
PROiQ3UV74.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000157.
CleanExiMM_ITGB2L.
ExpressionAtlasiQ3UV74. baseline and differential.
GenevisibleiQ3UV74. MM.

Family and domain databases

Gene3Di3.40.50.410. 1 hit.
InterProiIPR033760. Integin_beta_N.
IPR015812. Integrin_bsu.
IPR012896. Integrin_bsu_tail.
IPR002369. Integrin_bsu_VWA.
IPR032695. Integrin_dom.
IPR016201. PSI.
IPR002035. VWF_A.
[Graphical view]
PANTHERiPTHR10082. PTHR10082. 1 hit.
PfamiPF00362. Integrin_beta. 2 hits.
PF17205. PSI_integrin. 1 hit.
[Graphical view]
PRINTSiPR01186. INTEGRINB.
SMARTiSM00187. INB. 1 hit.
SM01242. Integrin_B_tail. 1 hit.
SM00423. PSI. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
SSF69179. SSF69179. 1 hit.
SSF69687. SSF69687. 1 hit.
PROSITEiPS00022. EGF_1. 2 hits.
PS01186. EGF_2. 3 hits.
PS00243. INTEGRIN_BETA. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITB2L_MOUSE
AccessioniPrimary (citable) accession number: Q3UV74
Secondary accession number(s): O88424, Q2KHL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The human orthologous protein seems not to exist.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.