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Protein

GPI inositol-deacylase

Gene

Pgap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in inositol deacylation of GPI-anchored proteins. GPI inositol deacylation may important for efficient transport of GPI-anchored proteins from the endoplasmic reticulum to the Golgi (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei174By similarity1

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior axis specification Source: MGI
  • embryonic pattern specification Source: MGI
  • forebrain regionalization Source: MGI
  • GPI anchor biosynthetic process Source: Ensembl
  • head development Source: MGI
  • myo-inositol transport Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-162791. Attachment of GPI anchor to uPAR.

Protein family/group databases

ESTHERimouse-q3uuq7. PGAP1.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI inositol-deacylase (EC:3.1.-.-)
Alternative name(s):
Post-GPI attachment to proteins factor 1
Gene namesi
Name:Pgap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443342. Pgap1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 597LumenalSequence analysisAdd BLAST565
Transmembranei598 – 618HelicalSequence analysisAdd BLAST21
Topological domaini619 – 641CytoplasmicSequence analysisAdd BLAST23
Transmembranei642 – 662HelicalSequence analysisAdd BLAST21
Topological domaini663 – 668LumenalSequence analysis6
Transmembranei669 – 689HelicalSequence analysisAdd BLAST21
Topological domaini690 – 694CytoplasmicSequence analysis5
Transmembranei695 – 715HelicalSequence analysisAdd BLAST21
Topological domaini716 – 733LumenalSequence analysisAdd BLAST18
Transmembranei734 – 754HelicalSequence analysisAdd BLAST21
Topological domaini755 – 817CytoplasmicSequence analysisAdd BLAST63
Transmembranei818 – 838HelicalSequence analysisAdd BLAST21
Topological domaini839 – 894LumenalSequence analysisAdd BLAST56
Transmembranei895 – 915HelicalSequence analysisAdd BLAST21
Topological domaini916 – 922CytoplasmicSequence analysis7

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3259507.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776241 – 922GPI inositol-deacylaseAdd BLAST922

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi363N-linked (GlcNAc...)Sequence analysis1
Glycosylationi402N-linked (GlcNAc...)Sequence analysis1
Glycosylationi558N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3UUQ7.
PeptideAtlasiQ3UUQ7.
PRIDEiQ3UUQ7.

PTM databases

iPTMnetiQ3UUQ7.
PhosphoSitePlusiQ3UUQ7.

Expressioni

Gene expression databases

BgeeiENSMUSG00000073678.
CleanExiMM_PGAP1.
GenevisibleiQ3UUQ7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095346.

Structurei

3D structure databases

ProteinModelPortaliQ3UUQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI inositol-deacylase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3724. Eukaryota.
ENOG410YD9N. LUCA.
GeneTreeiENSGT00390000016484.
HOGENOMiHOG000090006.
HOVERGENiHBG082122.
InParanoidiQ3UUQ7.
KOiK05294.
OMAiVIHCAAI.
OrthoDBiEOG091G06G8.
TreeFamiTF314565.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR012908. PGAP1-like.
[Graphical view]
PfamiPF07819. PGAP1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UUQ7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFLHSVNLWN LAFYVFMVFL ATLGLWDVFF GFEENKCSMS YMFEYPEYQK
60 70 80 90 100
IELPKKLTKR YPAYELYLYG EGSYAEEHKI LPLTGIPVLF LPGNAGSYKQ
110 120 130 140 150
VRSIGSIALR KAEDIDFKYH FDFFSVNFNE ELVALYGGSL QKQTKFVHEC
160 170 180 190 200
IKAILKLYKG QEFAPTSVAI IGHSMGGLVA RALLTLKNFK QDLINLLVTQ
210 220 230 240 250
ATPHVAPVMP LDRFITEFYM NVNNYWILNA RHINLTTLSV AGGFRDYQVR
260 270 280 290 300
SGLTFLPKLS HYTSALSVVS SAVPKTWVST DHLSIVWCKQ LQLTTIRAFF
310 320 330 340 350
DLIDADTKQI TQKPKKKLSV LNHHFIRHPA KQFEENPSII SDLTGTSMWV
360 370 380 390 400
PVKVSRWSYV AYNESDKIYF AFPLANHRKI YTHAYCQSTM LDTNSWIFGC
410 420 430 440 450
INSTSMCRQG VDLSWKAELL PTIKSLTLRL QDYPSLSHIV VYVPSVHGSK
460 470 480 490 500
FVVDCEFFKK EARSMQLPVT HLFSFGLSSR KVTLNTNGLY YNIELLNFGQ
510 520 530 540 550
IYQAFKVNVV SKCTGSKEEI TSIYKLHIPW SYEDSLTIAQ VPSSTDISLK
560 570 580 590 600
LHVAQPENDS HVALLKMYTS SDCQYEVTIK TSFPQILGQV VRFHGGALPA
610 620 630 640 650
YVVSSILLAY GGQLYSLLST GYCLEYSTIL DKEAKPYKVD PFVIMIKFLL
660 670 680 690 700
GYKWFKELWD AVLLPELDAI VLTSQSMCFP LVSLILFLFG TCTAYWSGLL
710 720 730 740 750
SSTSVQLLSS LWLALKRPAE LPKDIKVMSP DLPVLTVVFL IVSWTTCGAL
760 770 780 790 800
AILLSYLYYV FKVVHLQASL TTFKNNQPVN PKHSRRSEKK SNHHKDSAVQ
810 820 830 840 850
SLRLCANDAE DSLRMHSTVI NLLTWVVLLS MPSLIYWLKN LRYYFKLSPD
860 870 880 890 900
PCKPLAFLLI PAIAILGNTH TVSVKSSKLL KTVSQFPLPL AVGVIAFGSS
910 920
HLYRVPCFVI IPLVFHALCN FM
Length:922
Mass (Da):104,578
Last modified:July 27, 2011 - v3
Checksum:iE503CA512882F943
GO
Isoform 2 (identifier: Q3UUQ7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-166: KIELPKKLTK...LYKGQEFAPT → VRPSLTCAPT...VAKADLKKRL
     167-922: Missing.

Note: No experimental confirmation available.
Show »
Length:166
Mass (Da):18,488
Checksum:i23862438C15C3B39
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223N → D in BAE23568 (PubMed:16141072).Curated1
Sequence conflicti576E → V in BAE23568 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02304550 – 166KIELP…EFAPT → VRPSLTCAPTPPGLLSGSDG TLRLGALLALEVSCFVLGRA SSPPRPLVPHPDSGPRLLTA REPALVPLPNPYTSRTLLCL QGDDFSSRPVMGVRQISLFW KEAASCRVAKADLKKRL in isoform 2. 1 PublicationAdd BLAST117
Alternative sequenceiVSP_023046167 – 922Missing in isoform 2. 1 PublicationAdd BLAST756

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051360 mRNA. Translation: BAC34614.1.
AK138171 mRNA. Translation: BAE23568.1.
AC121980 Genomic DNA. No translation available.
AC124538 Genomic DNA. No translation available.
CCDSiCCDS48260.1. [Q3UUQ7-1]
RefSeqiNP_001156786.1. NM_001163314.2. [Q3UUQ7-1]
UniGeneiMm.103539.

Genome annotation databases

EnsembliENSMUST00000097739; ENSMUSP00000095346; ENSMUSG00000073678. [Q3UUQ7-1]
GeneIDi241062.
KEGGimmu:241062.
UCSCiuc011wky.2. mouse. [Q3UUQ7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051360 mRNA. Translation: BAC34614.1.
AK138171 mRNA. Translation: BAE23568.1.
AC121980 Genomic DNA. No translation available.
AC124538 Genomic DNA. No translation available.
CCDSiCCDS48260.1. [Q3UUQ7-1]
RefSeqiNP_001156786.1. NM_001163314.2. [Q3UUQ7-1]
UniGeneiMm.103539.

3D structure databases

ProteinModelPortaliQ3UUQ7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000095346.

Chemistry databases

ChEMBLiCHEMBL3259507.

Protein family/group databases

ESTHERimouse-q3uuq7. PGAP1.

PTM databases

iPTMnetiQ3UUQ7.
PhosphoSitePlusiQ3UUQ7.

Proteomic databases

PaxDbiQ3UUQ7.
PeptideAtlasiQ3UUQ7.
PRIDEiQ3UUQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000097739; ENSMUSP00000095346; ENSMUSG00000073678. [Q3UUQ7-1]
GeneIDi241062.
KEGGimmu:241062.
UCSCiuc011wky.2. mouse. [Q3UUQ7-1]

Organism-specific databases

CTDi80055.
MGIiMGI:2443342. Pgap1.

Phylogenomic databases

eggNOGiKOG3724. Eukaryota.
ENOG410YD9N. LUCA.
GeneTreeiENSGT00390000016484.
HOGENOMiHOG000090006.
HOVERGENiHBG082122.
InParanoidiQ3UUQ7.
KOiK05294.
OMAiVIHCAAI.
OrthoDBiEOG091G06G8.
TreeFamiTF314565.

Enzyme and pathway databases

ReactomeiR-MMU-162791. Attachment of GPI anchor to uPAR.

Miscellaneous databases

PROiQ3UUQ7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000073678.
CleanExiMM_PGAP1.
GenevisibleiQ3UUQ7. MM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR012908. PGAP1-like.
[Graphical view]
PfamiPF07819. PGAP1. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGAP1_MOUSE
AccessioniPrimary (citable) accession number: Q3UUQ7
Secondary accession number(s): E9QKG7, Q8BQ77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 6, 2007
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.