Q3UUJ4 (STRAA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
STE20-related kinase adapter protein alpha
Short name=STRAD alpha

Alternative name(s):
STE20-related adapter protein

Gene names

Name:	Strada
Synonyms:	Lyk5, Strad

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation By similarity.
Subunit structure	Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity.
Domain	The protein kinase domain is predicted to be catalytically inactive.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of protein kinase activity Inferred from sequence or structural similarity. Source: UniProtKB cell cycle Inferred from electronic annotation. Source: UniProtKB-KW protein export from nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro protein kinase activator activity Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine kinase activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.2 (identifier: Q3UUJ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q3UUJ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     5-41: Missing.
     370-381: Missing.

Note: No experimental confirmation available.

Isoform 3 Ref.1 Ref.3 (identifier: Q3UUJ4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     5-41: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	STE20-related kinase adapter protein alpha		PRO_0000260037
Regions
	Domain	69 – 379	311	Protein kinase
Amino acid modifications
	Modified residue	419	1	Phosphothreonine; by LKB1 By similarity UniProtKB Q7RTN6
Natural variations
	Alternative sequence	5 – 41	37	Missing in isoform 2 and isoform 3. Ref.1 Ref.2 Ref.3		VSP_052217
	Alternative sequence	370 – 381	12	Missing in isoform 2. Ref.1		VSP_052218

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 6E4B14CC9B2B1D7D	FASTA	431	48,236
	10 20 30 40 50 60 MSFLVSKPER IRRWVSEKFI VEGLRDLELF GEQPPGDTRR KANEASSESI ASFSKPEMMS 70 80 90 100 110 120 SFLPEGGCYE LLTIIGKGFE DLMTVNLARY KPTGEYVTVR RINLEACSNE MVTFLQGELH 130 140 150 160 170 180 VSKLFSHPNI VPYRATFIAD NELWVVTSFM AYGSAKDLIG THFMDGMNEL AIAYILQGVL 190 200 210 220 230 240 KALDYIHHMG YVHRSVKASH ILISTDGKVY LSGLRSNLSM ISHGQRQRAV HDFPKYSIKV 250 260 270 280 290 300 LPWLSPEVLQ QNLQGYDAKS DIYSVGITAC ELANGHVPFK DMPATQMLLE KLNGTVPCLL 310 320 330 340 350 360 DTSTIPAEEL TMSPSRSIAN PGLNDSLAAG SLRPSNGDSP SHPYHRTFSP HFHNFVEQCL 370 380 390 400 410 420 QRNPDARPNA STLLNHSFFK QIKRRASEAL PELLRPVTPI TNFEGSQSQD HSGIFGLVTN 430 LEDLEVDDWE F
	Isoform 2 [UniParc]. Checksum: 9EFCD966627136AA	FASTA	382	42,454
	10 20 30 40 50 60 MSFLANEASS ESIASFSKPE MMSSFLPEGG CYELLTIIGK GFEDLMTVNL ARYKPTGEYV 70 80 90 100 110 120 TVRRINLEAC SNEMVTFLQG ELHVSKLFSH PNIVPYRATF IADNELWVVT SFMAYGSAKD 130 140 150 160 170 180 LIGTHFMDGM NELAIAYILQ GVLKALDYIH HMGYVHRSVK ASHILISTDG KVYLSGLRSN 190 200 210 220 230 240 LSMISHGQRQ RAVHDFPKYS IKVLPWLSPE VLQQNLQGYD AKSDIYSVGI TACELANGHV 250 260 270 280 290 300 PFKDMPATQM LLEKLNGTVP CLLDTSTIPA EELTMSPSRS IANPGLNDSL AAGSLRPSNG 310 320 330 340 350 360 DSPSHPYHRT FSPHFHNFVE QCLQRNPDAR PNIKRRASEA LPELLRPVTP ITNFEGSQSQ 370 380 DHSGIFGLVT NLEDLEVDDW EF
	Isoform 3 [UniParc]. Checksum: C743BFE801ADDB8D	FASTA	394	43,829
	10 20 30 40 50 60 MSFLANEASS ESIASFSKPE MMSSFLPEGG CYELLTIIGK GFEDLMTVNL ARYKPTGEYV 70 80 90 100 110 120 TVRRINLEAC SNEMVTFLQG ELHVSKLFSH PNIVPYRATF IADNELWVVT SFMAYGSAKD 130 140 150 160 170 180 LIGTHFMDGM NELAIAYILQ GVLKALDYIH HMGYVHRSVK ASHILISTDG KVYLSGLRSN 190 200 210 220 230 240 LSMISHGQRQ RAVHDFPKYS IKVLPWLSPE VLQQNLQGYD AKSDIYSVGI TACELANGHV 250 260 270 280 290 300 PFKDMPATQM LLEKLNGTVP CLLDTSTIPA EELTMSPSRS IANPGLNDSL AAGSLRPSNG 310 320 330 340 350 360 DSPSHPYHRT FSPHFHNFVE QCLQRNPDAR PNASTLLNHS FFKQIKRRAS EALPELLRPV 370 380 390 TPITNFEGSQ SQDHSGIFGL VTNLEDLEVD DWEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of mouse protein kinase LYK5." Zhou G., Wang J., Zhang Y. Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 3). Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3). Strain: C57BL/6J. Tissue: Embryo and Hypothalamus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY341872 mRNA. Translation: AAQ24157.1. AY341878 mRNA. Translation: AAQ24162.1. AK011451 mRNA. Translation: BAB27626.1. AK138358 mRNA. Translation: BAE23631.1. BC058517 mRNA. Translation: AAH58517.1.
IPI	IPI00649437. IPI00649998. IPI00807906.
RefSeq	NP_001239377.1. NM_001252448.1. NP_001239378.1. NM_001252449.1. NP_082402.1. NM_028126.3.
UniGene	Mm.403722.
3D structure databases
ProteinModelPortal	Q3UUJ4.
SMR	Q3UUJ4. Positions 60-431.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q3UUJ4. 1 interaction.
PTM databases
PhosphoSite	Q3UUJ4.
Proteomic databases
PaxDb	Q3UUJ4.
PRIDE	Q3UUJ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000007444; ENSMUSP00000007444; ENSMUSG00000069631. ENSMUST00000103072; ENSMUSP00000099361; ENSMUSG00000069631.
GeneID	72149.
KEGG	mmu:72149.
UCSC	uc011ygi.1. mouse.
Organism-specific databases
CTD	92335.
MGI	MGI:1919399. Strada.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000099731.
HOGENOM	HOG000237355.
HOVERGEN	HBG055069.
InParanoid	Q3UUJ4.
KO	K08271.
OMA	NGTVPCL.
OrthoDB	EOG4WSW9P.
Gene expression databases
ArrayExpress	Q3UUJ4.
Bgee	Q3UUJ4.
CleanEx	MM_2610019A05RIK.
Genevestigator	Q3UUJ4.
GermOnline	ENSMUSG00000069631. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50011. PROTEIN_KINASE_DOM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	335570.
SOURCE	Search...

Entry information

Entry name

STRAA_MOUSE

Accession

Primary (citable) accession number: Q3UUJ4
Secondary accession number(s): Q6VEU7, Q9D0G8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2006
Last sequence update:	October 11, 2005
Last modified:	April 3, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program