Q3UTQ8 (CDKL5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase-like 5
EC=2.7.11.22

Gene names

Name:

Cdkl5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	938 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Mediates phosphorylation of MECP2 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with MECP2 By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein autophosphorylation Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC kinase activity Inferred from sequence orthology PubMed 15917271. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q3UTQ8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3UTQ8-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-401: Missing.
     402-410: IPHLLSPKE → MPLIPASKK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 938	938	Cyclin-dependent kinase-like 5		PRO_0000275897
Regions
	Domain	13 – 297	285	Protein kinase
	Nucleotide binding	19 – 27	9	ATP By similarity
	Compositional bias	784 – 789	6	Poly-Lys
Sites
	Active site	135	1	Proton acceptor By similarity
	Binding site	42	1	ATP By similarity
Amino acid modifications
	Modified residue	407	1	Phosphoserine By similarity
	Modified residue	720	1	Phosphoserine By similarity
	Modified residue	761	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 401	401	Missing in isoform 2.		VSP_022968
	Alternative sequence	402 – 410	9	IPHLLSPKE → MPLIPASKK in isoform 2.		VSP_022969

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 11, 2005. Version 1. Checksum: 3502580AF9A48DDD	FASTA	938	105,489
	10 20 30 40 50 60 MKIPNIGNVM NKFEILGVVG EGAYGVVLKC RHKETHEIVA IKKFKDSEEN EEVKETTLRE 70 80 90 100 110 120 LKMLRTLKQE NIVELKEAFR RRGKLYLVFE YVEKNMLELL EEMPNGVPPE KVKSYIYQLI 130 140 150 160 170 180 KAIHWCHKND IVHRDIKPEN LLISHNDVLK LCDFGFARNL SEGNNANYTE YVATRWYRSP 190 200 210 220 230 240 ELLLGAPYGK SVDMWSVGCI LGELSDGQPL FPGESEIDQL FTIQKVLGPL PSEQMKLFYS 250 260 270 280 290 300 NPRFHGLRFP AVNHPQSLER RYLGILNSVL LDLMKNLLKL DPADRYLTEQ CLNHPTFQTQ 310 320 330 340 350 360 RLLDRSPSRS TKRKPYHVES STLSNRNQST KGAALQTHHR SNSKDIQNLS VGLPRAEEGL 370 380 390 400 410 420 PANESFLNGN LAGATLSPMH TKTYQASTQP GSSSKDLTNN NIPHLLSPKE AKSKTEFDFN 430 440 450 460 470 480 IDTKPSEGPG TKYLKSSTRS QQNRHSFMES SQSKAGTLQP SEKQSRHSYI DTIPQSSRSP 490 500 510 520 530 540 SYRTKAKSHG ALSDSKSVSN LSEARAQITE TNTSRYFPSS CLDLNSPTSP TPTRHTDTRT 550 560 570 580 590 600 LLSPSGRNNR NEGTLDSRRT TTRHSKTMEE LKLPEHMDSS HSHSLSAPHE SFSYGLGYTS 610 620 630 640 650 660 PFSSQQRPHR HSMYVTRDKV RAKGLDGSLS IGQGMAARAN SLQLLSPQPG EQLPPEMTVA 670 680 690 700 710 720 RPSVKESSRE GASSFHTRQK SEGGVYHDPH SDDGTAPKEN RHLYNDPVPR RVGSFYRVPS 730 740 750 760 770 780 PRPDNSFHEN NVSTRVSSLP SDSSSGTNHS KRQPGFDPWK SPENISHADQ LKEKEKQGFF 790 800 810 820 830 840 RSMKKKKKKT QTVPNTDGPD LLTLQKAIHS SSTASSRPKE WRPEKLSDLQ TQSQPLKSLR 850 860 870 880 890 900 KLLHLSSSTN HPASSDPRFQ PLTAQQAKNS FSEIRIHPLS QATGGSSNIR QEPTPKGRPA 910 920 930 LQLPGSSLLR YNGWKHSRSR SSQPDEVIFL ASHEKWKQ
	Isoform 2 [UniParc]. Checksum: B47FF2787C0E643E	FASTA	537	59,784
	10 20 30 40 50 60 MPLIPASKKA KSKTEFDFNI DTKPSEGPGT KYLKSSTRSQ QNRHSFMESS QSKAGTLQPS 70 80 90 100 110 120 EKQSRHSYID TIPQSSRSPS YRTKAKSHGA LSDSKSVSNL SEARAQITET NTSRYFPSSC 130 140 150 160 170 180 LDLNSPTSPT PTRHTDTRTL LSPSGRNNRN EGTLDSRRTT TRHSKTMEEL KLPEHMDSSH 190 200 210 220 230 240 SHSLSAPHES FSYGLGYTSP FSSQQRPHRH SMYVTRDKVR AKGLDGSLSI GQGMAARANS 250 260 270 280 290 300 LQLLSPQPGE QLPPEMTVAR PSVKESSREG ASSFHTRQKS EGGVYHDPHS DDGTAPKENR 310 320 330 340 350 360 HLYNDPVPRR VGSFYRVPSP RPDNSFHENN VSTRVSSLPS DSSSGTNHSK RQPGFDPWKS 370 380 390 400 410 420 PENISHADQL KEKEKQGFFR SMKKKKKKTQ TVPNTDGPDL LTLQKAIHSS STASSRPKEW 430 440 450 460 470 480 RPEKLSDLQT QSQPLKSLRK LLHLSSSTNH PASSDPRFQP LTAQQAKNSF SEIRIHPLSQ 490 500 510 520 530 ATGGSSNIRQ EPTPKGRPAL QLPGSSLLRY NGWKHSRSRS SQPDEVIFLA SHEKWKQ

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Cerebellum and Heart.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-788 (ISOFORM 2). Strain: Czech II. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK052380 mRNA. Translation: BAC34965.1. AK139220 mRNA. Translation: BAE23922.1. BC042705 mRNA. Translation: AAH42705.1.
IPI	IPI00381416. IPI00828430.
RefSeq	NP_001019795.1. NM_001024624.2.
UniGene	Mm.443717.
3D structure databases
ProteinModelPortal	Q3UTQ8.
SMR	Q3UTQ8. Positions 10-343.
ModBase	Search...
PTM databases
PhosphoSite	Q3UTQ8.
Proteomic databases
PaxDb	Q3UTQ8.
PRIDE	Q3UTQ8.
Protocols and materials databases
DNASU	382253.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000087104; ENSMUSP00000084342; ENSMUSG00000031292.
GeneID	382253.
KEGG	mmu:382253.
UCSC	uc009utr.2. mouse.
Organism-specific databases
CTD	6792.
MGI	MGI:1278336. Cdkl5.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00650000093115.
HOGENOM	HOG000049181.
HOVERGEN	HBG050863.
InParanoid	Q3UTQ8.
KO	K08824.
OrthoDB	EOG43R3M2.
Gene expression databases
ArrayExpress	Q3UTQ8.
Bgee	Q3UTQ8.
CleanEx	MM_CDKL5.
Genevestigator	Q3UTQ8.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	403189.
SOURCE	Search...

Entry information

Entry name

CDKL5_MOUSE

Accession

Primary (citable) accession number: Q3UTQ8
Secondary accession number(s): Q05BK3, Q8BWI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 6, 2007
Last sequence update:	October 11, 2005
Last modified:	May 29, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program