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Protein

Rho guanine nucleotide exchange factor 9

Gene

Arhgef9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: MGI
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • regulation of Rho protein signal transduction Source: InterPro
  • small GTPase mediated signal transduction Source: MGI

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648 NRAGE signals death through JNK
R-MMU-194840 Rho GTPase cycle
R-MMU-416482 G alpha (12/13) signalling events
R-MMU-977441 GABA A receptor activation

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 9
Alternative name(s):
Collybistin
Rac/Cdc42 guanine nucleotide exchange factor 9
Gene namesi
Name:Arhgef9
Synonyms:Kiaa0424
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442233 Arhgef9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002538961 – 516Rho guanine nucleotide exchange factor 9Add BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei502PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3UTH8
PeptideAtlasiQ3UTH8
PRIDEiQ3UTH8

PTM databases

iPTMnetiQ3UTH8
PhosphoSitePlusiQ3UTH8

Expressioni

Tissue specificityi

Detected in embryonic and adult brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000025656
CleanExiMM_ARHGEF9
ExpressionAtlasiQ3UTH8 baseline and differential
GenevisibleiQ3UTH8 MM

Interactioni

Subunit structurei

Interacts with GPHN.By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: MGI
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109510

Structurei

3D structure databases

ProteinModelPortaliQ3UTH8
SMRiQ3UTH8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 67SH3PROSITE-ProRule annotationAdd BLAST60
Domaini103 – 287DHPROSITE-ProRule annotationAdd BLAST185
Domaini318 – 425PHPROSITE-ProRule annotationAdd BLAST108

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 110Interaction with GPHNBy similarityAdd BLAST11

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3519 Eukaryota
ENOG410XT9S LUCA
GeneTreeiENSGT00900000140796
HOVERGENiHBG050568
InParanoidiQ3UTH8
KOiK20686
PhylomeDBiQ3UTH8

Family and domain databases

CDDicd00160 RhoGEF, 1 hit
cd11975 SH3_ARHGEF9, 1 hit
Gene3Di1.20.900.10, 1 hit
2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR035728 ARHGEF9_SH3
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PfamiView protein in Pfam
PF00169 PH, 1 hit
PF00621 RhoGEF, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00233 PH, 1 hit
SM00325 RhoGEF, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS50010 DH_2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50002 SH3, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UTH8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYDLITGDSI VSAEAVWDHV TMANRELAFK AGDVIKVLDA SNKDWWWGQI
60 70 80 90 100
DDEEGWFPAS FVRLWVNQED GVEEGPSDVQ NGHLDPNSDC LCLGRPLQNR
110 120 130 140 150
DQMRANVINE IMSTERHYIK HLKDICEGYL KQCRKRRDMF SDEQLKVIFG
160 170 180 190 200
NIEDIYRFQM GFVRDLEKQY NNDDPHLSEI GPCFLEHQDG FWIYSEYCNN
210 220 230 240 250
HLDACMELSK LMKDSRYQHF FEACRLLQQM IDIAIDGFLL TPVQKICKYP
260 270 280 290 300
LQLAELLKYT AQDHSDYRYV AAALAVMRNV TQQINERKRR LENIDKIAQW
310 320 330 340 350
QASVLDWEGE DILDRSSELI YTGEMAWIYQ PYGRNQQRVF FLFDHQMVLC
360 370 380 390 400
KKDLIRRDIL YYKGRIDMDK YEVIDIEDGR DDDFNVSMKN AFKLHNKETE
410 420 430 440 450
EVHLFFAKKL EEKIRWLRAF REERKMVQED EKIGFEISEN QKRQAAMTVR
460 470 480 490 500
KASKQKGVNS ARSVPPSYPP PQDPLNQGQY LVPDGIAQSQ VFEFTEPKRS
510
QSPFWQNFSR LTPFKK
Length:516
Mass (Da):60,927
Last modified:October 11, 2005 - v1
Checksum:iA3372AA20B1B8F0B
GO
Isoform 2 (identifier: Q3UTH8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Show »
Length:495
Mass (Da):58,623
Checksum:i732B2EAB16C5435A
GO
Isoform 3 (identifier: Q3UTH8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MYD → MQWIRGGTGM
     458-516: VNSARSVPPS...NFSRLTPFKK → TSAFTPPNPLTVCVGLGNHGVCVFTFLGSFLY

Show »
Length:496
Mass (Da):58,260
Checksum:i8CEE5D9C3591F66B
GO
Isoform 4 (identifier: Q3UTH8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MYD → MTL

Show »
Length:516
Mass (Da):60,863
Checksum:iA33559F9F3EB8F0B
GO

Sequence cautioni

The sequence BAC65562 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0211431 – 21Missing in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_0211441 – 3MYD → MQWIRGGTGM in isoform 3. 1 Publication3
Alternative sequenceiVSP_0211451 – 3MYD → MTL in isoform 4. 1 Publication3
Alternative sequenceiVSP_021146458 – 516VNSAR…TPFKK → TSAFTPPNPLTVCVGLGNHG VCVFTFLGSFLY in isoform 3. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122280 mRNA Translation: BAC65562.1 Different initiation.
AK038840 mRNA Translation: BAC30147.2 Different termination.
AK163874 mRNA Translation: BAE37525.1
AK139416 mRNA Translation: BAE24002.1
BX470092, AL807821 Genomic DNA Translation: CAM18133.1
BX470092, AL807821 Genomic DNA Translation: CAM18135.1
AL807821, BX470092 Genomic DNA Translation: CAM19851.1
AL807821, BX470092 Genomic DNA Translation: CAM19853.1
BC141385 mRNA Translation: AAI41386.1
CCDSiCCDS53137.1 [Q3UTH8-1]
CCDS72409.1 [Q3UTH8-2]
RefSeqiNP_001028501.1, NM_001033329.3 [Q3UTH8-1]
NP_001277313.1, NM_001290384.1 [Q3UTH8-2]
NP_001277314.1, NM_001290385.1 [Q3UTH8-2]
XP_011245884.1, XM_011247582.2 [Q3UTH8-4]
XP_011245885.1, XM_011247583.2 [Q3UTH8-1]
XP_017173962.1, XM_017318473.1 [Q3UTH8-2]
UniGeneiMm.44841

Genome annotation databases

EnsembliENSMUST00000113876; ENSMUSP00000109508; ENSMUSG00000025656 [Q3UTH8-2]
ENSMUST00000113878; ENSMUSP00000109510; ENSMUSG00000025656 [Q3UTH8-1]
ENSMUST00000113882; ENSMUSP00000109514; ENSMUSG00000025656 [Q3UTH8-4]
ENSMUST00000113883; ENSMUSP00000109515; ENSMUSG00000025656 [Q3UTH8-4]
ENSMUST00000181987; ENSMUSP00000138461; ENSMUSG00000025656 [Q3UTH8-3]
ENSMUST00000182001; ENSMUSP00000138668; ENSMUSG00000025656 [Q3UTH8-2]
ENSMUST00000199920; ENSMUSP00000143779; ENSMUSG00000025656 [Q3UTH8-2]
GeneIDi236915
KEGGimmu:236915
UCSCiuc009ttt.2 mouse [Q3UTH8-1]
uc009ttv.1 mouse [Q3UTH8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiARHG9_MOUSE
AccessioniPrimary (citable) accession number: Q3UTH8
Secondary accession number(s): B1AXI3
, B1AXI5, B9EJ88, Q3TQ60, Q80U06, Q8CAF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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