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Protein

Rho guanine nucleotide exchange factor 9

Gene

Arhgef9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for CDC42. Promotes formation of GPHN clusters (By similarity).By similarity

GO - Molecular functioni

  • guanyl-nucleotide exchange factor activity Source: MGI
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • regulation of Rho protein signal transduction Source: InterPro
  • small GTPase mediated signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.
R-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 9
Alternative name(s):
Collybistin
Rac/Cdc42 guanine nucleotide exchange factor 9
Gene namesi
Name:Arhgef9
Synonyms:Kiaa0424
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2442233. Arhgef9.

Subcellular locationi

GO - Cellular componenti

  • cell cortex Source: MGI
  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 516516Rho guanine nucleotide exchange factor 9PRO_0000253896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei502 – 5021PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3UTH8.
MaxQBiQ3UTH8.
PaxDbiQ3UTH8.
PeptideAtlasiQ3UTH8.
PRIDEiQ3UTH8.

PTM databases

iPTMnetiQ3UTH8.
PhosphoSiteiQ3UTH8.

Expressioni

Tissue specificityi

Detected in embryonic and adult brain.1 Publication

Gene expression databases

BgeeiQ3UTH8.
CleanExiMM_ARHGEF9.
ExpressionAtlasiQ3UTH8. baseline and differential.
GenevisibleiQ3UTH8. MM.

Interactioni

Subunit structurei

Interacts with GPHN.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109510.

Structurei

3D structure databases

ProteinModelPortaliQ3UTH8.
SMRiQ3UTH8. Positions 10-454.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 6760SH3PROSITE-ProRule annotationAdd
BLAST
Domaini103 – 287185DHPROSITE-ProRule annotationAdd
BLAST
Domaini318 – 425108PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni100 – 11011Interaction with GPHNBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiKOG3519. Eukaryota.
ENOG410XT9S. LUCA.
GeneTreeiENSGT00760000118925.
HOVERGENiHBG050568.
InParanoidiQ3UTH8.
PhylomeDBiQ3UTH8.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UTH8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYDLITGDSI VSAEAVWDHV TMANRELAFK AGDVIKVLDA SNKDWWWGQI
60 70 80 90 100
DDEEGWFPAS FVRLWVNQED GVEEGPSDVQ NGHLDPNSDC LCLGRPLQNR
110 120 130 140 150
DQMRANVINE IMSTERHYIK HLKDICEGYL KQCRKRRDMF SDEQLKVIFG
160 170 180 190 200
NIEDIYRFQM GFVRDLEKQY NNDDPHLSEI GPCFLEHQDG FWIYSEYCNN
210 220 230 240 250
HLDACMELSK LMKDSRYQHF FEACRLLQQM IDIAIDGFLL TPVQKICKYP
260 270 280 290 300
LQLAELLKYT AQDHSDYRYV AAALAVMRNV TQQINERKRR LENIDKIAQW
310 320 330 340 350
QASVLDWEGE DILDRSSELI YTGEMAWIYQ PYGRNQQRVF FLFDHQMVLC
360 370 380 390 400
KKDLIRRDIL YYKGRIDMDK YEVIDIEDGR DDDFNVSMKN AFKLHNKETE
410 420 430 440 450
EVHLFFAKKL EEKIRWLRAF REERKMVQED EKIGFEISEN QKRQAAMTVR
460 470 480 490 500
KASKQKGVNS ARSVPPSYPP PQDPLNQGQY LVPDGIAQSQ VFEFTEPKRS
510
QSPFWQNFSR LTPFKK
Length:516
Mass (Da):60,927
Last modified:October 11, 2005 - v1
Checksum:iA3372AA20B1B8F0B
GO
Isoform 2 (identifier: Q3UTH8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Show »
Length:495
Mass (Da):58,623
Checksum:i732B2EAB16C5435A
GO
Isoform 3 (identifier: Q3UTH8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MYD → MQWIRGGTGM
     458-516: VNSARSVPPS...NFSRLTPFKK → TSAFTPPNPLTVCVGLGNHGVCVFTFLGSFLY

Show »
Length:496
Mass (Da):58,260
Checksum:i8CEE5D9C3591F66B
GO
Isoform 4 (identifier: Q3UTH8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-3: MYD → MTL

Show »
Length:516
Mass (Da):60,863
Checksum:iA33559F9F3EB8F0B
GO

Sequence cautioni

The sequence BAC65562.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2121Missing in isoform 2. 1 PublicationVSP_021143Add
BLAST
Alternative sequencei1 – 33MYD → MQWIRGGTGM in isoform 3. 1 PublicationVSP_021144
Alternative sequencei1 – 33MYD → MTL in isoform 4. 1 PublicationVSP_021145
Alternative sequencei458 – 51659VNSAR…TPFKK → TSAFTPPNPLTVCVGLGNHG VCVFTFLGSFLY in isoform 3. 1 PublicationVSP_021146Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122280 mRNA. Translation: BAC65562.1. Different initiation.
AK038840 mRNA. Translation: BAC30147.2. Different termination.
AK163874 mRNA. Translation: BAE37525.1.
AK139416 mRNA. Translation: BAE24002.1.
BX470092, AL807821 Genomic DNA. Translation: CAM18133.1.
BX470092, AL807821 Genomic DNA. Translation: CAM18135.1.
AL807821, BX470092 Genomic DNA. Translation: CAM19851.1.
AL807821, BX470092 Genomic DNA. Translation: CAM19853.1.
BC141385 mRNA. Translation: AAI41386.1.
CCDSiCCDS53137.1. [Q3UTH8-1]
CCDS72409.1. [Q3UTH8-2]
RefSeqiNP_001028501.1. NM_001033329.3. [Q3UTH8-1]
NP_001277313.1. NM_001290384.1. [Q3UTH8-2]
NP_001277314.1. NM_001290385.1. [Q3UTH8-2]
XP_011245884.1. XM_011247582.1. [Q3UTH8-4]
XP_011245885.1. XM_011247583.1. [Q3UTH8-1]
UniGeneiMm.44841.

Genome annotation databases

EnsembliENSMUST00000113876; ENSMUSP00000109508; ENSMUSG00000025656. [Q3UTH8-2]
ENSMUST00000113878; ENSMUSP00000109510; ENSMUSG00000025656. [Q3UTH8-1]
ENSMUST00000113882; ENSMUSP00000109514; ENSMUSG00000025656. [Q3UTH8-4]
ENSMUST00000113883; ENSMUSP00000109515; ENSMUSG00000025656. [Q3UTH8-4]
ENSMUST00000181987; ENSMUSP00000138461; ENSMUSG00000025656. [Q3UTH8-3]
ENSMUST00000182001; ENSMUSP00000138668; ENSMUSG00000025656. [Q3UTH8-2]
ENSMUST00000199920; ENSMUSP00000143779; ENSMUSG00000025656. [Q3UTH8-2]
GeneIDi236915.
KEGGimmu:236915.
UCSCiuc009ttt.2. mouse. [Q3UTH8-1]
uc009ttv.1. mouse. [Q3UTH8-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK122280 mRNA. Translation: BAC65562.1. Different initiation.
AK038840 mRNA. Translation: BAC30147.2. Different termination.
AK163874 mRNA. Translation: BAE37525.1.
AK139416 mRNA. Translation: BAE24002.1.
BX470092, AL807821 Genomic DNA. Translation: CAM18133.1.
BX470092, AL807821 Genomic DNA. Translation: CAM18135.1.
AL807821, BX470092 Genomic DNA. Translation: CAM19851.1.
AL807821, BX470092 Genomic DNA. Translation: CAM19853.1.
BC141385 mRNA. Translation: AAI41386.1.
CCDSiCCDS53137.1. [Q3UTH8-1]
CCDS72409.1. [Q3UTH8-2]
RefSeqiNP_001028501.1. NM_001033329.3. [Q3UTH8-1]
NP_001277313.1. NM_001290384.1. [Q3UTH8-2]
NP_001277314.1. NM_001290385.1. [Q3UTH8-2]
XP_011245884.1. XM_011247582.1. [Q3UTH8-4]
XP_011245885.1. XM_011247583.1. [Q3UTH8-1]
UniGeneiMm.44841.

3D structure databases

ProteinModelPortaliQ3UTH8.
SMRiQ3UTH8. Positions 10-454.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000109510.

PTM databases

iPTMnetiQ3UTH8.
PhosphoSiteiQ3UTH8.

Proteomic databases

EPDiQ3UTH8.
MaxQBiQ3UTH8.
PaxDbiQ3UTH8.
PeptideAtlasiQ3UTH8.
PRIDEiQ3UTH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113876; ENSMUSP00000109508; ENSMUSG00000025656. [Q3UTH8-2]
ENSMUST00000113878; ENSMUSP00000109510; ENSMUSG00000025656. [Q3UTH8-1]
ENSMUST00000113882; ENSMUSP00000109514; ENSMUSG00000025656. [Q3UTH8-4]
ENSMUST00000113883; ENSMUSP00000109515; ENSMUSG00000025656. [Q3UTH8-4]
ENSMUST00000181987; ENSMUSP00000138461; ENSMUSG00000025656. [Q3UTH8-3]
ENSMUST00000182001; ENSMUSP00000138668; ENSMUSG00000025656. [Q3UTH8-2]
ENSMUST00000199920; ENSMUSP00000143779; ENSMUSG00000025656. [Q3UTH8-2]
GeneIDi236915.
KEGGimmu:236915.
UCSCiuc009ttt.2. mouse. [Q3UTH8-1]
uc009ttv.1. mouse. [Q3UTH8-3]

Organism-specific databases

CTDi23229.
MGIiMGI:2442233. Arhgef9.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3519. Eukaryota.
ENOG410XT9S. LUCA.
GeneTreeiENSGT00760000118925.
HOVERGENiHBG050568.
InParanoidiQ3UTH8.
PhylomeDBiQ3UTH8.

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-194840. Rho GTPase cycle.
R-MMU-416482. G alpha (12/13) signalling events.
R-MMU-975298. Ligand-gated ion channel transport.
R-MMU-977441. GABA A receptor activation.

Miscellaneous databases

ChiTaRSiArhgef9. mouse.
PROiQ3UTH8.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UTH8.
CleanExiMM_ARHGEF9.
ExpressionAtlasiQ3UTH8. baseline and differential.
GenevisibleiQ3UTH8. MM.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00621. RhoGEF. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Brain cortex, Cerebellum and Hypothalamus.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  5. "Distribution of transcripts for the brain-specific GDP/GTP exchange factor collybistin in the developing mouse brain."
    Kneussel M., Engelkamp D., Betz H.
    Eur. J. Neurosci. 13:487-492(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-502, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiARHG9_MOUSE
AccessioniPrimary (citable) accession number: Q3UTH8
Secondary accession number(s): B1AXI3
, B1AXI5, B9EJ88, Q3TQ60, Q80U06, Q8CAF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.