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Protein

Epithelial splicing regulatory protein 1

Gene

Esrp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: MGI
  • branching involved in salivary gland morphogenesis Source: MGI
  • epithelial tube branching involved in lung morphogenesis Source: MGI
  • positive regulation of epithelial cell proliferation Source: MGI
  • regulation of RNA splicing Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial splicing regulatory protein 1
Alternative name(s):
RNA-binding motif protein 35A
RNA-binding protein 35A
Gene namesi
Name:Esrp1
Synonyms:Rbm35a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1917326. Esrp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002730471 – 680Epithelial splicing regulatory protein 1Add BLAST680

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei542PhosphoserineBy similarity1
Modified residuei581Omega-N-methylarginineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ3US41.
PaxDbiQ3US41.
PeptideAtlasiQ3US41.
PRIDEiQ3US41.

PTM databases

PhosphoSitePlusiQ3US41.

Expressioni

Tissue specificityi

Epithelial cell-specific. Epithelial-specific expression in diverse tissues and organs with particularly notable levels of expression in skin and gastrointestinal epithelia.1 Publication

Gene expression databases

BgeeiENSMUSG00000040728.
CleanExiMM_RBM35A.
ExpressionAtlasiQ3US41. baseline and differential.
GenevisibleiQ3US41. MM.

Interactioni

Protein-protein interaction databases

BioGridi228933. 1 interactor.
STRINGi10090.ENSMUSP00000103947.

Structurei

3D structure databases

ProteinModelPortaliQ3US41.
SMRiQ3US41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 301RRM 1PROSITE-ProRule annotationAdd BLAST78
Domaini325 – 405RRM 2PROSITE-ProRule annotationAdd BLAST81
Domaini444 – 524RRM 3PROSITE-ProRule annotationAdd BLAST81

Sequence similaritiesi

Belongs to the ESRP family.Curated
Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1365. Eukaryota.
ENOG410XPZU. LUCA.
GeneTreeiENSGT00760000119102.
InParanoidiQ3US41.
KOiK14947.
PhylomeDBiQ3US41.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012337. RNaseH-like_dom.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
SSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3US41-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTASPDYLVV LFGITAGATG AKLGSDEKEL ILLLWKVVDL ANKKVGQLHE
60 70 80 90 100
VLVRPDQLEL TEDCKEETKI DAENLSSAPQ LDQALRQFNQ SVSNELNIGV
110 120 130 140 150
GTSFCLCTDG QLHIRQILHP EASKKNVLLP ECFYSFFDLR KEFKKCCPGS
160 170 180 190 200
PDIDKLDVAA MTESLNFEKS DSVSRYGASQ VEDMGNIILA MISEPYNHRF
210 220 230 240 250
SDPERVNYKF ESGTCKMELI DDSTVVRARG LPWQSSDQDI ARFFKGLNIA
260 270 280 290 300
KGGAALCLNA QGRRNGEALV RFVSEEHRDL ALQRHKHHMG TRYIEVYKAT
310 320 330 340 350
GEDFLKIAGG TSNEVAQFLS KENQVIVRMR GLPFTATAEE VVAFFGQHCP
360 370 380 390 400
ITGGKEGILF VTYPDGRPTG DAFVLFACEE YAQNALRKHK ELLGKRYIEL
410 420 430 440 450
FRSTAAEVQQ VLNRFSSAPL IPLPTPPIIP VLPQQFVPPT NVRDCIRLRG
460 470 480 490 500
LPYAATIEDI LDFLGEFSTD IRTHGVHMVL NHQGRPSGDA FIQMKSTDRA
510 520 530 540 550
FMAAQKYHKK TMKDRYVEVF QCSAEEMNFV LMGGTLNRNG LSPPPCKLPC
560 570 580 590 600
LSPPSYTFPA PTAVIPTEAA IYQPSLLLNP RALQPSTAYY PAGTQLFMNY
610 620 630 640 650
TAYYPSPPGS PNSLGYFPTA ANLSSVPPQP GTVVRMQGLA YNTGVKEILN
660 670 680
FFQGYQYATE DGLVHTNDQA RTLPKEWVCI
Length:680
Mass (Da):75,549
Last modified:May 5, 2009 - v2
Checksum:i972BF5D6AFB5FFD4
GO
Isoform 2 (identifier: Q3US41-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-215: C → CS
     607-607: P → V
     608-680: Missing.

Show »
Length:608
Mass (Da):67,699
Checksum:i8C5BBF61DE635165
GO
Isoform 3 (identifier: Q3US41-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-215: C → CS
     545-548: Missing.
     607-607: P → V
     608-680: Missing.

Note: No experimental confirmation available.
Show »
Length:604
Mass (Da):67,258
Checksum:iB1CCA1CDC497AECA
GO
Isoform 4 (identifier: Q3US41-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-215: C → CS
     484-513: GRPSGDAFIQMKSTDRAFMAAQKYHKKTMK → VRGVIGKCVCSFLRPSAPSSITAQRFTLPL
     514-680: Missing.

Note: No experimental confirmation available.
Show »
Length:514
Mass (Da):57,166
Checksum:iE52B30F889C68E8A
GO
Isoform 5 (identifier: Q3US41-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     215-215: C → CS
     545-548: Missing.

Note: No experimental confirmation available.
Show »
Length:677
Mass (Da):75,195
Checksum:iA64DE9B768A94849
GO

Sequence cautioni

The sequence CAM20812 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM20813 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM20814 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM20816 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence EDL05650 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113H → N in BAE24496 (PubMed:16141072).Curated1
Sequence conflicti127V → L in AAH31468 (PubMed:15489334).Curated1
Sequence conflicti206V → L in AAH31468 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_022468215C → CS in isoform 2, isoform 3, isoform 4 and isoform 5. 2 Publications1
Alternative sequenceiVSP_022469484 – 513GRPSG…KKTMK → VRGVIGKCVCSFLRPSAPSS ITAQRFTLPL in isoform 4. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_022470514 – 680Missing in isoform 4. 1 PublicationAdd BLAST167
Alternative sequenceiVSP_022471545 – 548Missing in isoform 3 and isoform 5. 1 Publication4
Alternative sequenceiVSP_022472607P → V in isoform 2 and isoform 3. 1 Publication1
Alternative sequenceiVSP_022473608 – 680Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST73

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042164 mRNA. Translation: BAC31189.1.
AK140848 mRNA. Translation: BAE24496.1.
AK166376 mRNA. Translation: BAE38739.1.
AL772170 Genomic DNA. Translation: CAM20812.1. Sequence problems.
AL772170 Genomic DNA. Translation: CAM20813.1. Sequence problems.
AL772170 Genomic DNA. Translation: CAM20814.1. Sequence problems.
AL772170 Genomic DNA. Translation: CAM20815.1.
AL772170 Genomic DNA. Translation: CAM20816.1. Sequence problems.
CH466538 Genomic DNA. Translation: EDL05650.1. Sequence problems.
BC031468 mRNA. Translation: AAH31468.1.
BC059280 mRNA. Translation: AAH59280.1.
RefSeqiNP_001277312.1. NM_001290383.1. [Q3US41-1]
NP_918944.2. NM_194055.3.
XP_006537778.1. XM_006537715.1. [Q3US41-5]
XP_006537781.1. XM_006537718.1. [Q3US41-2]
XP_006537783.1. XM_006537720.1. [Q3US41-3]
UniGeneiMm.387719.

Genome annotation databases

EnsembliENSMUST00000108310; ENSMUSP00000103946; ENSMUSG00000040728. [Q3US41-5]
ENSMUST00000108313; ENSMUSP00000103949; ENSMUSG00000040728. [Q3US41-3]
GeneIDi207920.
KEGGimmu:207920.
UCSCiuc008rzk.3. mouse. [Q3US41-3]
uc008rzl.3. mouse. [Q3US41-2]
uc008rzm.3. mouse. [Q3US41-1]
uc008rzn.1. mouse. [Q3US41-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK042164 mRNA. Translation: BAC31189.1.
AK140848 mRNA. Translation: BAE24496.1.
AK166376 mRNA. Translation: BAE38739.1.
AL772170 Genomic DNA. Translation: CAM20812.1. Sequence problems.
AL772170 Genomic DNA. Translation: CAM20813.1. Sequence problems.
AL772170 Genomic DNA. Translation: CAM20814.1. Sequence problems.
AL772170 Genomic DNA. Translation: CAM20815.1.
AL772170 Genomic DNA. Translation: CAM20816.1. Sequence problems.
CH466538 Genomic DNA. Translation: EDL05650.1. Sequence problems.
BC031468 mRNA. Translation: AAH31468.1.
BC059280 mRNA. Translation: AAH59280.1.
RefSeqiNP_001277312.1. NM_001290383.1. [Q3US41-1]
NP_918944.2. NM_194055.3.
XP_006537778.1. XM_006537715.1. [Q3US41-5]
XP_006537781.1. XM_006537718.1. [Q3US41-2]
XP_006537783.1. XM_006537720.1. [Q3US41-3]
UniGeneiMm.387719.

3D structure databases

ProteinModelPortaliQ3US41.
SMRiQ3US41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi228933. 1 interactor.
STRINGi10090.ENSMUSP00000103947.

PTM databases

PhosphoSitePlusiQ3US41.

Proteomic databases

MaxQBiQ3US41.
PaxDbiQ3US41.
PeptideAtlasiQ3US41.
PRIDEiQ3US41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108310; ENSMUSP00000103946; ENSMUSG00000040728. [Q3US41-5]
ENSMUST00000108313; ENSMUSP00000103949; ENSMUSG00000040728. [Q3US41-3]
GeneIDi207920.
KEGGimmu:207920.
UCSCiuc008rzk.3. mouse. [Q3US41-3]
uc008rzl.3. mouse. [Q3US41-2]
uc008rzm.3. mouse. [Q3US41-1]
uc008rzn.1. mouse. [Q3US41-4]

Organism-specific databases

CTDi54845.
MGIiMGI:1917326. Esrp1.

Phylogenomic databases

eggNOGiKOG1365. Eukaryota.
ENOG410XPZU. LUCA.
GeneTreeiENSGT00760000119102.
InParanoidiQ3US41.
KOiK14947.
PhylomeDBiQ3US41.

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.

Miscellaneous databases

PROiQ3US41.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040728.
CleanExiMM_RBM35A.
ExpressionAtlasiQ3US41. baseline and differential.
GenevisibleiQ3US41. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR012337. RNaseH-like_dom.
IPR000504. RRM_dom.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
SSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiESRP1_MOUSE
AccessioniPrimary (citable) accession number: Q3US41
Secondary accession number(s): A2AJQ4
, A2AJQ5, A2AJQ6, A2AJQ7, A2AJQ8, Q3TLQ3, Q6PCL4, Q8C9F5, Q8K0G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: May 5, 2009
Last modified: November 2, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.