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Protein

Transforming growth factor-beta receptor-associated protein 1

Gene

Tgfbrap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the TGF-beta/activin signaling pathway. It associates with inactive heteromeric TGF-beta and activin receptor complexes, mainly through the type II receptor, and is released upon activation of signaling. May recruit SMAD4 to the vicinity of the receptor complex and facilitate its interaction with receptor-regulated Smads, such as SMAD2 (By similarity).By similarity
Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes and in degradative but not recycling trafficking of endocytosed cargo (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Transforming growth factor-beta receptor-associated protein 1
Short name:
TGF-beta receptor-associated protein 1
Short name:
TRAP-1
Short name:
TRAP1
Gene namesi
Name:Tgfbrap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2447427. Tgfbrap1.

Subcellular locationi

  • Cytoplasm
  • Early endosome By similarity

  • Note: Colocalizes with TGF-beta receptors in the absence of signaling.By similarity

GO - Cellular componenti

  • CORVET complex Source: UniProtKB
  • early endosome Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal, both before blastula stage or during gastrulation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 860860Transforming growth factor-beta receptor-associated protein 1PRO_0000345406Add
BLAST

Proteomic databases

EPDiQ3UR70.
MaxQBiQ3UR70.
PaxDbiQ3UR70.
PRIDEiQ3UR70.

PTM databases

iPTMnetiQ3UR70.
PhosphoSiteiQ3UR70.

Expressioni

Gene expression databases

BgeeiQ3UR70.
ExpressionAtlasiQ3UR70. baseline and differential.
GenevisibleiQ3UR70. MM.

Interactioni

Subunit structurei

Interacts with TGFBR2 and ACVR2B; in the absence of ligand stimulation. Interacts with TGFBR1, ACVRL1, BMPR1A and ACVR1B; in the absence of ligand stimulation and to a less extent. Interacts with SMAD4; the interaction seems to be mutually exclusive with the interaction of SMAD4 and phosphorylated SMAD2. May interact with ALOX5 (By similarity). Interacts with RAB5C. Interacts with VPS8, VPS11 and VPS16. Component of the putative class C core vacuaole/endosome tethering (CORVET) complex; the core of which composed of the class C Vps proteins VPS11, VPS16, VPS18 and VPS33A, is associated with VPS8 and TGFBRAP1 (PubMed:25266290).By similarity1 Publication1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215781. 1 interaction.
IntActiQ3UR70. 1 interaction.
STRINGi10090.ENSMUSP00000092624.

Structurei

3D structure databases

ProteinModelPortaliQ3UR70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 297274CNHPROSITE-ProRule annotationAdd
BLAST
Repeati564 – 732169CHCRAdd
BLAST

Sequence similaritiesi

Belongs to the TRAP1 family.Curated
Contains 1 CHCR (clathrin heavy-chain) repeat.PROSITE-ProRule annotation
Contains 1 CNH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2063. Eukaryota.
ENOG410XNSX. LUCA.
GeneTreeiENSGT00530000063596.
HOGENOMiHOG000007291.
HOVERGENiHBG108563.
InParanoidiQ3UR70.
OMAiVECCGRD.
OrthoDBiEOG7RBZ7Q.
PhylomeDBiQ3UR70.
TreeFamiTF328650.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR001180. CNH_dom.
IPR019452. VPS39/TGF_beta_rcpt-assoc_1.
IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
[Graphical view]
PfamiPF00637. Clathrin. 1 hit.
PF00780. CNH. 1 hit.
PF10366. Vps39_1. 1 hit.
PF10367. Vps39_2. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
PS50219. CNH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UR70-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMNIKAFTLV SAVERELLMG DRDHISIECV ECCGRNLYVG TNDCFIYHFL
60 70 80 90 100
LEEKAMPTGT ATFVATKQLH RHLGFKKPVN ELCAASALNR LLVLCDNSIT
110 120 130 140 150
LVNMLNLEPV PSGARIKGAT TFAVNESPVN GDPFCVEVCI ISVKRRTVQM
160 170 180 190 200
FLVYEDRVQI VKEVSTPEQP LAVAVDGYFL CLALTTQYII LNYSTGLSQD
210 220 230 240 250
LFPYCSEEKP PIVKRIGRQE FLLAGPGGLG MFATVAGISQ RAPVHWSENV
260 270 280 290 300
IGAAVCFPYV IALDDEFITV HSMLDQQQKQ TLPFKEGHIL QDFEGRVIVA
310 320 330 340 350
TSKGVYILVP LPLEKQIQDL LANRRVEEAL VLAKGARRNI PKEKFQVMYR
360 370 380 390 400
RILQQAGFIQ FAQLQFLEAK ELFRSSQLDV RELISLYPFL LPTSSSFTRS
410 420 430 440 450
HPPLHEYADL NQLTQGDQEK MAKCKRFLMS YLNEIRSTEV ANGYKEDIDT
460 470 480 490 500
ALLKLYAEAD HDSLLDLLVT ENFCLLTDSA AWLEKHKKYF ALGLLYHYNK
510 520 530 540 550
QDASAVQLWV NIVNGDIQDS TRSDLYEYIV DFLTYCLDQE LVWTHADWLL
560 570 580 590 600
QKSEEIGVQI FTKRPLDEQQ QTSFNPDNII SSLKKYPKAL VKYLEHLVID
610 620 630 640 650
RRLQKEEYHT HLAILYLEEV LRQRVSTGGK DVEATETQAK LRRLLQKSDL
660 670 680 690 700
YRVHLLKEKV QGAGLPMESA ILHGKLGEHE KALHILVHEM GDFSAAEDYC
710 720 730 740 750
LWSSEGQGAA CRQRLFHTLL AMYLRAGPSA QDLTVAAVDL LNHHAREFDV
760 770 780 790 800
TQVLQLLPDT WSVQLLCPFL MGAMRDSIHA RRTTQVALGL AKSENLIYMY
810 820 830 840 850
DKMKLKGNAV RLSERELCQL CQNPFGEPVF VRYPNGGLVH THCAASRHTA
860
PSSPSPGTRT
Length:860
Mass (Da):97,303
Last modified:October 11, 2005 - v1
Checksum:iB86BD77735D04C7B
GO
Isoform 2 (identifier: Q3UR70-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-247: MFATVAGISQRAPVHWS → EEGWGSLLMAPASCLLS
     248-860: Missing.

Note: No experimental confirmation available.
Show »
Length:247
Mass (Da):27,199
Checksum:i829B1480CAF29146
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei231 – 24717MFATV…PVHWS → EEGWGSLLMAPASCLLS in isoform 2. 1 PublicationVSP_034944Add
BLAST
Alternative sequencei248 – 860613Missing in isoform 2. 1 PublicationVSP_034945Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051333 mRNA. Translation: BAC34606.1.
AK141742 mRNA. Translation: BAE24818.1.
AK159332 mRNA. Translation: BAE34997.1.
CH466589 Genomic DNA. Translation: EDK96916.1.
BC138866 mRNA. Translation: AAI38867.1.
BC138867 mRNA. Translation: AAI38868.1.
CCDSiCCDS14920.1. [Q3UR70-1]
RefSeqiNP_001013043.1. NM_001013025.2. [Q3UR70-1]
XP_006496364.1. XM_006496301.2. [Q3UR70-1]
XP_006496365.1. XM_006496302.2. [Q3UR70-1]
XP_006496366.1. XM_006496303.2. [Q3UR70-1]
UniGeneiMm.246069.

Genome annotation databases

EnsembliENSMUST00000095014; ENSMUSP00000092624; ENSMUSG00000070939. [Q3UR70-1]
ENSMUST00000186694; ENSMUSP00000140132; ENSMUSG00000070939. [Q3UR70-1]
GeneIDi73122.
KEGGimmu:73122.
UCSCiuc007ave.1. mouse. [Q3UR70-1]
uc007avh.1. mouse. [Q3UR70-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK051333 mRNA. Translation: BAC34606.1.
AK141742 mRNA. Translation: BAE24818.1.
AK159332 mRNA. Translation: BAE34997.1.
CH466589 Genomic DNA. Translation: EDK96916.1.
BC138866 mRNA. Translation: AAI38867.1.
BC138867 mRNA. Translation: AAI38868.1.
CCDSiCCDS14920.1. [Q3UR70-1]
RefSeqiNP_001013043.1. NM_001013025.2. [Q3UR70-1]
XP_006496364.1. XM_006496301.2. [Q3UR70-1]
XP_006496365.1. XM_006496302.2. [Q3UR70-1]
XP_006496366.1. XM_006496303.2. [Q3UR70-1]
UniGeneiMm.246069.

3D structure databases

ProteinModelPortaliQ3UR70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215781. 1 interaction.
IntActiQ3UR70. 1 interaction.
STRINGi10090.ENSMUSP00000092624.

PTM databases

iPTMnetiQ3UR70.
PhosphoSiteiQ3UR70.

Proteomic databases

EPDiQ3UR70.
MaxQBiQ3UR70.
PaxDbiQ3UR70.
PRIDEiQ3UR70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095014; ENSMUSP00000092624; ENSMUSG00000070939. [Q3UR70-1]
ENSMUST00000186694; ENSMUSP00000140132; ENSMUSG00000070939. [Q3UR70-1]
GeneIDi73122.
KEGGimmu:73122.
UCSCiuc007ave.1. mouse. [Q3UR70-1]
uc007avh.1. mouse. [Q3UR70-2]

Organism-specific databases

CTDi9392.
MGIiMGI:2447427. Tgfbrap1.

Phylogenomic databases

eggNOGiKOG2063. Eukaryota.
ENOG410XNSX. LUCA.
GeneTreeiENSGT00530000063596.
HOGENOMiHOG000007291.
HOVERGENiHBG108563.
InParanoidiQ3UR70.
OMAiVECCGRD.
OrthoDBiEOG7RBZ7Q.
PhylomeDBiQ3UR70.
TreeFamiTF328650.

Miscellaneous databases

ChiTaRSiTgfbrap1. mouse.
PROiQ3UR70.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UR70.
ExpressionAtlasiQ3UR70. baseline and differential.
GenevisibleiQ3UR70. MM.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR001180. CNH_dom.
IPR019452. VPS39/TGF_beta_rcpt-assoc_1.
IPR019453. VPS39/TGF_beta_rcpt-assoc_2.
[Graphical view]
PfamiPF00637. Clathrin. 1 hit.
PF00780. CNH. 1 hit.
PF10366. Vps39_1. 1 hit.
PF10367. Vps39_2. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
PS50219. CNH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Osteoclast and Spinal ganglion.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Spleen and Testis.
  5. "The TGF-beta signaling modulators TRAP1/TGFBRAP1 and VPS39/Vam6/TLP are essential for early embryonic development."
    Messler S., Kropp S., Episkopou V., Felici A., Wurthner J., Lemke R., Jerabek-Willemsen M., Willecke R., Scheu S., Pfeffer K., Wurthner J.U.
    Immunobiology 216:343-350(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Mammalian CORVET is required for fusion and conversion of distinct early endosome subpopulations."
    Perini E.D., Schaefer R., Stoeter M., Kalaidzidis Y., Zerial M.
    Traffic 15:1366-1389(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAB5C, SUBUNIT.

Entry informationi

Entry nameiTGFA1_MOUSE
AccessioniPrimary (citable) accession number: Q3UR70
Secondary accession number(s): Q8BKN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 11, 2005
Last modified: June 8, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.