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Protein

P2X purinoceptor 3

Gene

P2rx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel.By similarity1 Publication

GO - Molecular functioni

  • ATP binding Source: Ensembl
  • extracellular ATP-gated cation channel activity Source: MGI
  • purinergic nucleotide receptor activity Source: GO_Central

GO - Biological processi

  • behavioral response to formalin induced pain Source: Ensembl
  • behavioral response to pain Source: MGI
  • cation transport Source: MGI
  • chemical synaptic transmission Source: MGI
  • ion transport Source: MGI
  • neuromuscular synaptic transmission Source: MGI
  • neuronal action potential Source: Ensembl
  • peristalsis Source: MGI
  • positive regulation of sensory perception of pain Source: Ensembl
  • protein homooligomerization Source: Ensembl
  • regulation of synaptic plasticity Source: MGI
  • response to ATP Source: MGI
  • response to carbohydrate Source: MGI
  • response to cold Source: MGI
  • response to heat Source: MGI
  • response to hypoxia Source: MGI
  • response to mechanical stimulus Source: MGI
  • response to organic substance Source: MGI
  • response to temperature stimulus Source: MGI
  • sensory perception of taste Source: MGI
  • urinary bladder smooth muscle contraction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-139853. Elevation of cytosolic Ca2+ levels.
R-MMU-418346. Platelet homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 3
Short name:
P2X3
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2rx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1097160. P2rx3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini44 – 317ExtracellularSequence analysisAdd BLAST274
Transmembranei318 – 338Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini339 – 397CytoplasmicSequence analysisAdd BLAST59

GO - Cellular componenti

  • axon Source: MGI
  • dendritic spine Source: Ensembl
  • Golgi apparatus Source: Ensembl
  • integral component of nuclear inner membrane Source: GO_Central
  • integral component of plasma membrane Source: MGI
  • membrane raft Source: Ensembl
  • neuronal cell body Source: Ensembl
  • neuron projection Source: MGI
  • receptor complex Source: MGI
  • rough endoplasmic reticulum Source: Ensembl
  • terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Simultaneous knockout of P2rx2 and P2rx3 results in reduced pain-related behaviors in response to intraplantar injection of formalin and reduced urinary bladder reflexes and decreased pelvic afferent nerve activity in response to bladder distension. Neurons have minimal to no response to ATP (PubMed:15961431). Simultaneous knockout of P2rx2 and P2rx3 results in defects in taste responses in the taste nerves and reduced behavioral responses to sweeteners, glutamate and bitter substances (PubMed:16322458).2 Publications

Chemistry databases

ChEMBLiCHEMBL2401608.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002691951 – 397P2X purinoceptor 3Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi107 ↔ 153By similarity
Disulfide bondi116 ↔ 137By similarity
Disulfide bondi122 ↔ 147By similarity
Glycosylationi139N-linked (GlcNAc...)Sequence analysis1
Glycosylationi170N-linked (GlcNAc...)Sequence analysis1
Glycosylationi194N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi203 ↔ 213By similarity
Disulfide bondi247 ↔ 256By similarity
Glycosylationi290N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ3UR32.
PaxDbiQ3UR32.
PRIDEiQ3UR32.

PTM databases

iPTMnetiQ3UR32.
PhosphoSitePlusiQ3UR32.

Expressioni

Gene expression databases

BgeeiENSMUSG00000027071.
ExpressionAtlasiQ3UR32. baseline and differential.
GenevisibleiQ3UR32. MM.

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028465.

Structurei

3D structure databases

ProteinModelPortaliQ3UR32.
SMRiQ3UR32.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ3UR32.
KOiK05217.
OMAiVLRTCEI.
OrthoDBiEOG091G0A2U.
PhylomeDBiQ3UR32.
TreeFamiTF328633.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003046. P2X3_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF8. PTHR10125:SF8. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01310. P2X3RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UR32-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNCISDFFTY ETTKSVVVKS WTIGIINRAV QLLIISYFVG WVFLHEKAYQ
60 70 80 90 100
VRDTAIESSV VTKVKGFGRY ANRVMDVSDY VTPPQGTSVF VIITKMIVTE
110 120 130 140 150
NQMQGFCPEN EEKYRCVSDS QCGPERFPGG GILTGRCVNY SSVRRTCEIQ
160 170 180 190 200
GWCPTEVDTV EMPIMMEAEN FTIFIKNSIR FPLFNFEKGN LLPNLTDKDI
210 220 230 240 250
KKCRFHPEKA PFCPILRVGD VVKFAGQDFA KLARTGGVLG IKIGWVCDLD
260 270 280 290 300
KAWDQCIPKY SFTRLDGVSE KSSVSPGYNF RFAKYYKMEN GSEYRTLLKA
310 320 330 340 350
FGIRFDVLVY GNAGKFNIIP TIISSVAAFT SVGVGTVLCD IILLNFLKGA
360 370 380 390
DHYKARKFEE VTETTLKGTA STNPVFTSDQ ATVEKQSTDS GAYSIGH
Length:397
Mass (Da):44,437
Last modified:October 11, 2005 - v1
Checksum:iBFB90C740E5DD910
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti295R → P in AAH23089 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140283 mRNA. Translation: BAE24316.1.
AK141847 mRNA. Translation: BAE24856.1.
AL935159 Genomic DNA. Translation: CAM18192.1.
BC023089 mRNA. Translation: AAH23089.1.
CCDSiCCDS16200.1.
RefSeqiNP_663501.2. NM_145526.2.
UniGeneiMm.440375.
Mm.448935.

Genome annotation databases

EnsembliENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071.
GeneIDi228139.
KEGGimmu:228139.
UCSCiuc008kjr.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK140283 mRNA. Translation: BAE24316.1.
AK141847 mRNA. Translation: BAE24856.1.
AL935159 Genomic DNA. Translation: CAM18192.1.
BC023089 mRNA. Translation: AAH23089.1.
CCDSiCCDS16200.1.
RefSeqiNP_663501.2. NM_145526.2.
UniGeneiMm.440375.
Mm.448935.

3D structure databases

ProteinModelPortaliQ3UR32.
SMRiQ3UR32.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000028465.

Chemistry databases

ChEMBLiCHEMBL2401608.

PTM databases

iPTMnetiQ3UR32.
PhosphoSitePlusiQ3UR32.

Proteomic databases

MaxQBiQ3UR32.
PaxDbiQ3UR32.
PRIDEiQ3UR32.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071.
GeneIDi228139.
KEGGimmu:228139.
UCSCiuc008kjr.1. mouse.

Organism-specific databases

CTDi5024.
MGIiMGI:1097160. P2rx3.

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiQ3UR32.
KOiK05217.
OMAiVLRTCEI.
OrthoDBiEOG091G0A2U.
PhylomeDBiQ3UR32.
TreeFamiTF328633.

Enzyme and pathway databases

ReactomeiR-MMU-139853. Elevation of cytosolic Ca2+ levels.
R-MMU-418346. Platelet homeostasis.

Miscellaneous databases

PROiQ3UR32.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027071.
ExpressionAtlasiQ3UR32. baseline and differential.
GenevisibleiQ3UR32. MM.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003046. P2X3_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF8. PTHR10125:SF8. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01310. P2X3RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2RX3_MOUSE
AccessioniPrimary (citable) accession number: Q3UR32
Secondary accession number(s): A2AW02, Q8R1U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.