Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q3UR32

- P2RX3_MOUSE

UniProt

Q3UR32 - P2RX3_MOUSE

Protein

P2X purinoceptor 3

Gene

P2rx3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Receptor for ATP that acts as a ligand-gated ion channel.By similarity

    GO - Molecular functioni

    1. ATP binding Source: Ensembl
    2. extracellular ATP-gated cation channel activity Source: MGI
    3. purinergic nucleotide receptor activity Source: RefGenome

    GO - Biological processi

    1. behavioral response to pain Source: MGI
    2. cation transport Source: MGI
    3. ion transport Source: MGI
    4. neuromuscular synaptic transmission Source: MGI
    5. neuronal action potential Source: Ensembl
    6. peristalsis Source: MGI
    7. protein homooligomerization Source: Ensembl
    8. purinergic nucleotide receptor signaling pathway Source: GOC
    9. regulation of synaptic plasticity Source: MGI
    10. response to ATP Source: MGI
    11. response to carbohydrate Source: MGI
    12. response to cold Source: MGI
    13. response to heat Source: MGI
    14. response to hypoxia Source: MGI
    15. response to mechanical stimulus Source: MGI
    16. response to organic substance Source: MGI
    17. response to temperature stimulus Source: MGI
    18. sensory perception of taste Source: MGI
    19. synaptic transmission Source: MGI
    20. urinary bladder smooth muscle contraction Source: MGI

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    P2X purinoceptor 3
    Short name:
    P2X3
    Alternative name(s):
    ATP receptor
    Purinergic receptor
    Gene namesi
    Name:P2rx3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1097160. P2rx3.

    Subcellular locationi

    Membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. axon Source: MGI
    2. dendritic spine Source: Ensembl
    3. Golgi apparatus Source: Ensembl
    4. integral component of nuclear inner membrane Source: RefGenome
    5. integral component of plasma membrane Source: MGI
    6. membrane raft Source: Ensembl
    7. neuronal cell body Source: Ensembl
    8. neuron projection Source: MGI
    9. receptor complex Source: MGI
    10. rough endoplasmic reticulum Source: Ensembl
    11. terminal bouton Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Simultaneous knockout of P2rx2 and P2rx3 results in reduced pain-related behaviors in response to intraplantar injection of formalin and reduced urinary bladder reflexes and decreased pelvic afferent nerve activity in response to bladder distension. Neurons have minimal to no response to ATP (PubMed:15961431). Simultaneous knockout of P2rx2 and P2rx3 results in defects in taste responses in the taste nerves and reduced behavioral responses to sweeteners, glutamate and bitter substances (PubMed:16322458).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397P2X purinoceptor 3PRO_0000269195Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi107 ↔ 153By similarity
    Disulfide bondi116 ↔ 137By similarity
    Disulfide bondi122 ↔ 147By similarity
    Glycosylationi139 – 1391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi194 – 1941N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi203 ↔ 213By similarity
    Disulfide bondi247 ↔ 256By similarity
    Glycosylationi290 – 2901N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ3UR32.

    PTM databases

    PhosphoSiteiQ3UR32.

    Expressioni

    Gene expression databases

    BgeeiQ3UR32.
    GenevestigatoriQ3UR32.

    Interactioni

    Subunit structurei

    Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UR32.
    SMRiQ3UR32. Positions 24-344.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2222CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini44 – 317274ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini339 – 39759CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4321Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei318 – 33821Helical; Name=2Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the P2X receptor family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40178.
    GeneTreeiENSGT00740000115538.
    HOGENOMiHOG000232042.
    HOVERGENiHBG053086.
    InParanoidiA2AW02.
    KOiK05217.
    OMAiVGWVFLH.
    OrthoDBiEOG78PV92.
    PhylomeDBiQ3UR32.
    TreeFamiTF328633.

    Family and domain databases

    Gene3Di2.60.490.10. 1 hit.
    InterProiIPR003046. P2X3_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view]
    PANTHERiPTHR10125. PTHR10125. 1 hit.
    PTHR10125:SF8. PTHR10125:SF8. 1 hit.
    PfamiPF00864. P2X_receptor. 1 hit.
    [Graphical view]
    PRINTSiPR01310. P2X3RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsiTIGR00863. P2X. 1 hit.
    PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3UR32-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNCISDFFTY ETTKSVVVKS WTIGIINRAV QLLIISYFVG WVFLHEKAYQ    50
    VRDTAIESSV VTKVKGFGRY ANRVMDVSDY VTPPQGTSVF VIITKMIVTE 100
    NQMQGFCPEN EEKYRCVSDS QCGPERFPGG GILTGRCVNY SSVRRTCEIQ 150
    GWCPTEVDTV EMPIMMEAEN FTIFIKNSIR FPLFNFEKGN LLPNLTDKDI 200
    KKCRFHPEKA PFCPILRVGD VVKFAGQDFA KLARTGGVLG IKIGWVCDLD 250
    KAWDQCIPKY SFTRLDGVSE KSSVSPGYNF RFAKYYKMEN GSEYRTLLKA 300
    FGIRFDVLVY GNAGKFNIIP TIISSVAAFT SVGVGTVLCD IILLNFLKGA 350
    DHYKARKFEE VTETTLKGTA STNPVFTSDQ ATVEKQSTDS GAYSIGH 397
    Length:397
    Mass (Da):44,437
    Last modified:October 11, 2005 - v1
    Checksum:iBFB90C740E5DD910
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti295 – 2951R → P in AAH23089. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK140283 mRNA. Translation: BAE24316.1.
    AK141847 mRNA. Translation: BAE24856.1.
    AL935159 Genomic DNA. Translation: CAM18192.1.
    BC023089 mRNA. Translation: AAH23089.1.
    CCDSiCCDS16200.1.
    RefSeqiNP_663501.2. NM_145526.2.
    UniGeneiMm.440375.
    Mm.448935.

    Genome annotation databases

    EnsembliENSMUST00000028465; ENSMUSP00000028465; ENSMUSG00000027071.
    GeneIDi228139.
    KEGGimmu:228139.
    UCSCiuc008kjr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK140283 mRNA. Translation: BAE24316.1 .
    AK141847 mRNA. Translation: BAE24856.1 .
    AL935159 Genomic DNA. Translation: CAM18192.1 .
    BC023089 mRNA. Translation: AAH23089.1 .
    CCDSi CCDS16200.1.
    RefSeqi NP_663501.2. NM_145526.2.
    UniGenei Mm.440375.
    Mm.448935.

    3D structure databases

    ProteinModelPortali Q3UR32.
    SMRi Q3UR32. Positions 24-344.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL2401608.
    GuidetoPHARMACOLOGYi 480.

    PTM databases

    PhosphoSitei Q3UR32.

    Proteomic databases

    PRIDEi Q3UR32.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028465 ; ENSMUSP00000028465 ; ENSMUSG00000027071 .
    GeneIDi 228139.
    KEGGi mmu:228139.
    UCSCi uc008kjr.1. mouse.

    Organism-specific databases

    CTDi 5024.
    MGIi MGI:1097160. P2rx3.

    Phylogenomic databases

    eggNOGi NOG40178.
    GeneTreei ENSGT00740000115538.
    HOGENOMi HOG000232042.
    HOVERGENi HBG053086.
    InParanoidi A2AW02.
    KOi K05217.
    OMAi VGWVFLH.
    OrthoDBi EOG78PV92.
    PhylomeDBi Q3UR32.
    TreeFami TF328633.

    Miscellaneous databases

    NextBioi 378939.
    PROi Q3UR32.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3UR32.
    Genevestigatori Q3UR32.

    Family and domain databases

    Gene3Di 2.60.490.10. 1 hit.
    InterProi IPR003046. P2X3_purnocptor.
    IPR027309. P2X_extracellular_dom.
    IPR001429. P2X_purnocptor.
    [Graphical view ]
    PANTHERi PTHR10125. PTHR10125. 1 hit.
    PTHR10125:SF8. PTHR10125:SF8. 1 hit.
    Pfami PF00864. P2X_receptor. 1 hit.
    [Graphical view ]
    PRINTSi PR01310. P2X3RECEPTOR.
    PR01307. P2XRECEPTOR.
    TIGRFAMsi TIGR00863. P2X. 1 hit.
    PROSITEi PS01212. P2X_RECEPTOR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina and Spinal ganglion.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    4. "P2X2 knockout mice and P2X2/P2X3 double knockout mice reveal a role for the P2X2 receptor subunit in mediating multiple sensory effects of ATP."
      Cockayne D.A., Dunn P.M., Zhong Y., Rong W., Hamilton S.G., Knight G.E., Ruan H.Z., Ma B., Yip P., Nunn P., McMahon S.B., Burnstock G., Ford A.P.
      J. Physiol. (Lond.) 567:621-639(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    5. "ATP signaling is crucial for communication from taste buds to gustatory nerves."
      Finger T.E., Danilova V., Barrows J., Bartel D.L., Vigers A.J., Stone L., Hellekant G., Kinnamon S.C.
      Science 310:1495-1499(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiP2RX3_MOUSE
    AccessioniPrimary (citable) accession number: Q3UR32
    Secondary accession number(s): A2AW02, Q8R1U4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 12, 2006
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3