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Protein

Kelch repeat and BTB domain-containing protein 8

Gene

Kbtbd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of neural crest specification. The BCR(KBTBD8) complex acts by mediating monoubiquitination of NOLC1 and TCOF1: monoubiquitination promotes the formation of a NOLC1-TCOF1 complex that acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification.By similarity

Caution

GO - Biological processi

Keywordsi

Biological processTranslation regulation, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch repeat and BTB domain-containing protein 8
Gene namesi
Name:Kbtbd8
Synonyms:Kiaa1842
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2661430 Kbtbd8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Golgi apparatus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002782211 – 599Kelch repeat and BTB domain-containing protein 8Add BLAST599

Proteomic databases

MaxQBiQ3UQV5
PaxDbiQ3UQV5
PeptideAtlasiQ3UQV5
PRIDEiQ3UQV5

PTM databases

iPTMnetiQ3UQV5
PhosphoSitePlusiQ3UQV5

Expressioni

Developmental stagei

Down-regulated in differentiating embryonic stem cells (ESCs).1 Publication

Gene expression databases

BgeeiENSMUSG00000030031
CleanExiMM_KBTBD8
ExpressionAtlasiQ3UQV5 baseline and differential
GenevisibleiQ3UQV5 MM

Interactioni

Subunit structurei

Component of the BCR(KBTBD8) E3 ubiquitin ligase complex, at least composed of CUL3, KBTBD8 and RBX1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032107

Structurei

3D structure databases

ProteinModelPortaliQ3UQV5
SMRiQ3UQV5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 117BTBPROSITE-ProRule annotationAdd BLAST69
Domaini152 – 254BACKAdd BLAST103
Repeati334 – 388Kelch 1Add BLAST55
Repeati389 – 439Kelch 2Add BLAST51
Repeati441 – 479Kelch 3Add BLAST39
Repeati481 – 530Kelch 4Add BLAST50
Repeati540 – 586Kelch 5Add BLAST47

Sequence similaritiesi

Belongs to the KBTBD8 family.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441 Eukaryota
ENOG410XNX8 LUCA
GeneTreeiENSGT00910000143979
HOGENOMiHOG000113200
HOVERGENiHBG100857
InParanoidiQ3UQV5
KOiK10475
OMAiRWFEHEQ
OrthoDBiEOG091G035Y
PhylomeDBiQ3UQV5
TreeFamiTF332672

Family and domain databases

Gene3Di2.120.10.801 hit
InterProiView protein in InterPro
IPR011705 BACK
IPR017096 BTB-kelch_protein
IPR000210 BTB/POZ_dom
IPR028764 KBTBD8
IPR015915 Kelch-typ_b-propeller
IPR006652 Kelch_1
IPR011333 SKP1/BTB/POZ_sf
PANTHERiPTHR24412:SF388 PTHR24412:SF388, 1 hit
PfamiView protein in Pfam
PF07707 BACK, 1 hit
PF00651 BTB, 1 hit
PF01344 Kelch_1, 3 hits
PIRSFiPIRSF037037 Kelch-like_protein_gigaxonin, 1 hit
SMARTiView protein in SMART
SM00875 BACK, 1 hit
SM00225 BTB, 1 hit
SM00612 Kelch, 3 hits
SUPFAMiSSF117281 SSF117281, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UQV5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASADLSKS SPTPNGIPSS DTANDTMDPF HACSILKQLK TMYDEGQLTD
60 70 80 90 100
IVVEVDHGKT FSCHRNVLAA ISPYFRSMFT SGLTESTQKE VRIIGVEAES
110 120 130 140 150
MDLVLNYAYT SRVILTEANV QALFTTASIF QIPSIQDQCA KYMISHLDPQ
160 170 180 190 200
NSIGVFIFAD HYGHQELGDR SKEYIRKKFL CVTKEQEFLQ LTKDQLISIL
210 220 230 240 250
DSDDLNVDRE EHVYESIIRW FEHEQSEREV HLPEIFAKCI RFPLMEDTFI
260 270 280 290 300
EKIPPQFAQA IVKSCGEPSN TSGCTQRLGM TASEMIICFD AAHKHSGKKQ
310 320 330 340 350
TVPCLDIVTG RVFKLCKPPN DLREVGILVS PDNDIYIAGG YRPSSSEVSI
360 370 380 390 400
DHKAENDFWM YDHSTNRWLS KPSLLRARIG CKLVYCCGKM YAIGGRVYEG
410 420 430 440 450
DGRNSLKSVE CYDSRENCWM TVCAMPVAME FHNAVEHKEK IYVLQGEFFL
460 470 480 490 500
FYEPQKDYWG FLTPMTVPRI QGLAAVYKDS IYYIAGTCGN HQRVFTVEAY
510 520 530 540 550
DIELNKWTRK KDFPCDQSIN PYLKLVLFQN KLHLFVRATQ VTVEEHIFRT
560 570 580 590
SRKNSLYQYD DIADQWMKVY ETPDRLWDLG RHFECAVAKL YPQCLQKVL
Length:599
Mass (Da):68,660
Last modified:October 11, 2005 - v1
Checksum:i822093A49ECF9960
GO
Isoform 2 (identifier: Q3UQV5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.

Note: No experimental confirmation available.
Show »
Length:522
Mass (Da):60,347
Checksum:i4A45733C947A405A
GO

Sequence cautioni

The sequence AAH88734 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti420M → V in BAE39583 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0231861 – 77Missing in isoform 2. 1 PublicationAdd BLAST77

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK142080 mRNA Translation: BAE24933.1
AK167507 mRNA Translation: BAE39583.1
BC088734 mRNA Translation: AAH88734.1 Different initiation.
AB093302 mRNA Translation: BAC41484.1
CCDSiCCDS51856.1 [Q3UQV5-1]
CCDS51857.1 [Q3UQV5-2]
RefSeqiNP_001008785.2, NM_001008785.5 [Q3UQV5-1]
NP_001096140.1, NM_001102670.2 [Q3UQV5-2]
UniGeneiMm.333275

Genome annotation databases

EnsembliENSMUST00000032107; ENSMUSP00000032107; ENSMUSG00000030031 [Q3UQV5-1]
ENSMUST00000119582; ENSMUSP00000113739; ENSMUSG00000030031 [Q3UQV5-2]
GeneIDi243574
KEGGimmu:243574
UCSCiuc009czw.3 mouse [Q3UQV5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKBTB8_MOUSE
AccessioniPrimary (citable) accession number: Q3UQV5
Secondary accession number(s): Q3TJB2, Q5M7B2, Q8CH97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: October 11, 2005
Last modified: April 25, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome