ID   SYTM_MOUSE              Reviewed;         723 AA.
AC   Q3UQ84; Q9D0D6;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   16-JUN-2009, entry version 34.
DE   RecName: Full=Threonyl-tRNA synthetase, mitochondrial;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonine--tRNA ligase;
DE            Short=ThrRS;
DE   AltName: Full=Threonyl-tRNA synthetase-like 1;
DE   Flags: Precursor;
GN   Name=Tars2; Synonyms=Tarsl1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
CC   -!- CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP +
CC       diphosphate + L-threonyl-tRNA(Thr).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; AK142685; BAE25159.1; -; mRNA.
DR   EMBL; AK011540; BAB27683.1; -; mRNA.
DR   IPI; IPI00132419; -.
DR   RefSeq; NP_082207.1; -.
DR   UniGene; Mm.271754; -.
DR   Ensembl; ENSMUSG00000028107; Mus musculus.
DR   GeneID; 71807; -.
DR   KEGG; mmu:71807; -.
DR   NMPDR; fig|10090.3.peg.8581; -.
DR   MGI; MGI:1919057; Tars2.
DR   HOVERGEN; Q3UQ84; -.
DR   OMA; Q3UQ84; GQKVDAV.
DR   BRENDA; 6.1.1.3; 244.
DR   NextBio; 334572; -.
DR   ArrayExpress; Q3UQ84; -.
DR   Bgee; Q3UQ84; -.
DR   GermOnline; ENSMUSG00000028107; Mus musculus.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:EC.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:InterPro.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-reg.
DR   InterPro; IPR006195; aa-tRNA-synth_II_cons-reg.
DR   InterPro; IPR004154; Anticodon_bd.
DR   InterPro; IPR012675; b-grasp_ferredoxin-like.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR002320; Thr-tRNA-synth_IIa.
DR   InterPro; IPR018158; Thr-tRNA-synth_IIa_cons-reg.
DR   InterPro; IPR012947; tRNA_SAD.
DR   Gene3D; G3DSA:3.40.50.800; Anticodon_bd; 1.
DR   Gene3D; G3DSA:3.10.20.30; Ferredoxin_fold; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF02824; TGS; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Transit peptide.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    723       Threonyl-tRNA synthetase, mitochondrial.
FT                                /FTId=PRO_0000254587.
FT   CONFLICT      9      9       R -> G (in Ref. 1; BAB27683).
SQ   SEQUENCE   723 AA;  81700 MW;  046BF1D524D1EDCA CRC64;
     MGLCLRWRRL GFPLPEFRRC ELHTVREASA PTPPHWLAER FGLFEELWTA HVKKLASMTQ
     KKARAIKISL PEGQKVDAVA WNTTPYQLAH QISVTLADTA VAAEVNGELY DLDRPLETDC
     HLRFLTFDSP EGKAVFWHSS AHVLGAAAEQ QLGAVLCRGP STESGFYHDF FLGKERTVRS
     AELPILERIC QELIAAAQPF RRLEASRDQL RQLFKDNHFK LHLIEEKVTG PTATVYGCGM
     SVDLCRGPHL RHTGQIGALK LLTNSSALWR SLGAPETLQR VSGISFPKVE LLRNWEARRE
     AAELRDHRRI GKEQELFFFH ELSPGSCFFL PRGTRVYNAL VAFIRAEYAR RGFSEVKTPT
     LFSTKLWEQS GHWEHYRADM FSLKPPGTDG VDNSQSGHPA RCPKDTLALK PMNCPAHCLM
     FAHRPRSWRE LPVRLADFGA LHRAEASGSL GGLTRLWRFQ QDDAHIFCAP HQLEAEIQGC
     LDFLRCVYSV LGFSFHLALS TRPPGFLGEP RLWDQAEQVL QQALEKFGEP WDLNPGDGAF
     YGPKIDVHLH DALGRPHQCG TIQLDFQLPL RFDLQYKGPA GTPECPVLIH RAVLGSVERL
     LGVLAESCGG KWPLWLSPLQ VVVIPVRTEQ EEYARQVQQC LQAAGLVSDL DADSGLTLSR
     RVRRAQLAHY NFQFVVGQRE QSQRTVNVRT RDNRQLGERD LAESVQRLLE LQNARVPNAE
     EVF
//