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Protein

ETS-related transcription factor Elf-3

Gene

Elf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter (By similarity). Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity (By similarity). Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway (By similarity). May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.By similarity4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi293 – 37583ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anatomical structure morphogenesis Source: MGI
  • blastocyst development Source: MGI
  • epithelial cell differentiation Source: UniProtKB
  • extracellular matrix organization Source: MGI
  • inflammatory response Source: UniProtKB
  • mammary gland involution Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Inflammatory response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-3
Alternative name(s):
E74-like factor 3
Epithelial-restricted with serine box
Epithelium-restricted Ets protein ESX
Epithelium-specific Ets transcription factor 1
Short name:
ESE-1
Gene namesi
Name:Elf3Imported
Synonyms:Ert, Esx, Jen
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1101781. Elf3.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show about 30% fetal lethality at around E11.5. Approximately 70% of the mutant progeny are born and display severe alterations in tissue architecture in the small intestine. Elf3-deficient enterocytes express markedly reduced levels of TGFBR2.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi153 – 1531D → A: Slight reduction in transactivation activity. 1 Publication
Mutagenesisi162 – 1621L → P: Significant loss of transactivation activity. 1 Publication
Mutagenesisi264 – 2674KRKR → AAAA: Abrogates nuclear localization activity. 1 Publication
Mutagenesisi269 – 2724RPRK → APAA: Clear and exclusive nuclear accumulation. 1 Publication
Mutagenesisi338 – 3403KKK → AAA: Abolishes nuclear localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391ETS-related transcription factor Elf-3PRO_0000287682Add
BLAST

Proteomic databases

MaxQBiQ3UPW2.
PaxDbiQ3UPW2.
PRIDEiQ3UPW2.

PTM databases

PhosphoSiteiQ3UPW2.

Expressioni

Tissue specificityi

Expressed in small intestine, colon, lung, kidney and uterus. Also expressed in the corneal epithelium and conjunctiva of the developing and adult eye. Not detected in liver, spleen, thymus, brain, heart, skeletal muscle or ovary.2 Publications

Developmental stagei

Expression increases progressively from E7 and is detectable in virgin mammary glands, then shows little if any change during pregnancy and declines to barely detectable levels after 3 days of lactation. Detected from E13.5 in conjunctiva epithelium. In cornea, a weak signal is detected at E16.5 and persists throughout the later stages of development.2 Publications

Inductioni

Expression in HC11 cells from midpregnant mouse mammary epithelium increases upon reaching lactogenic competency, and is down-regulated upon exposure to lactogenic hormones that induce milk protein (Beta-casein) expression. Up-regulated upon differentiation in corneal epithelium.2 Publications

Gene expression databases

BgeeiQ3UPW2.
CleanExiMM_ELF3.
GenevisibleiQ3UPW2. MM.

Interactioni

Subunit structurei

Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003135.

Structurei

Secondary structure

1
391
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi295 – 3039Combined sources
Helixi306 – 3083Combined sources
Beta strandi313 – 3175Combined sources
Turni318 – 3214Combined sources
Beta strandi322 – 3276Combined sources
Helixi328 – 33811Combined sources
Helixi346 – 3549Combined sources
Turni355 – 3606Combined sources
Beta strandi371 – 3744Combined sources
Turni383 – 3853Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JTGX-ray2.20A289-391[»]
ProteinModelPortaliQ3UPW2.
SMRiQ3UPW2. Positions 70-151, 292-386.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3UPW2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 15187PNTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.Sequence analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000232188.
HOVERGENiHBG074143.
InParanoidiQ3UPW2.
KOiK09429.
OMAiRDGFPDY.
OrthoDBiEOG7M0NRR.
PhylomeDBiQ3UPW2.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033074. Elf3.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF13. PTHR11849:SF13. 3 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3UPW2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATCEISNV FSNYFNAMYS SEDPTLAPAP PTTFGTEDLV LTLNNQQMTL
60 70 80 90 100
EGPGPQTRSQ RDRTDPLAVL HLAEKASWTS ERPQFWSKTQ VLEWISYQVE
110 120 130 140 150
KNKYDASSID FSRCDMDGAT LCSCALEELR LVFGPLGDQL HAQLRDLTSN
160 170 180 190 200
SSDELSWIIE LLEKDGMSFQ ESLGDSGPFD QGSPFAQELL DDGRQASPYY
210 220 230 240 250
CSTYGPGAPS PGSSDVSTAR TATPQSSHAS DSGGSDVDLD LTESKVFPRD
260 270 280 290 300
GFPDYKKGEP KHGKRKRGRP RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR
310 320 330 340 350
DILIHPELNE GLMKWENRHE GVFKFLRSEA VAQLWGQKKK NSNMTYEKLS
360 370 380 390
RAMRYYYKRE ILERVDGRRL VYKFGKNSSG WKEEEVGESR N
Length:391
Mass (Da):44,273
Last modified:October 11, 2005 - v1
Checksum:i738DA753961622D4
GO
Isoform 21 Publication (identifier: Q3UPW2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-73: Missing.

Show »
Length:371
Mass (Da):42,060
Checksum:i042EDACC05839EE6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei54 – 7320Missing in isoform 2. 2 PublicationsVSP_052434Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016294 mRNA. Translation: AAB96585.1.
AK143135 mRNA. Translation: BAE25282.1.
BC138692 mRNA. Translation: AAI38693.1.
BC145380 mRNA. Translation: AAI45381.1.
CCDSiCCDS48372.1. [Q3UPW2-1]
CCDS78689.1. [Q3UPW2-2]
RefSeqiNP_001156603.1. NM_001163131.1. [Q3UPW2-1]
NP_031947.1. NM_007921.3. [Q3UPW2-2]
UniGeneiMm.441439.

Genome annotation databases

EnsembliENSMUST00000003135; ENSMUSP00000003135; ENSMUSG00000003051. [Q3UPW2-1]
ENSMUST00000185752; ENSMUSP00000139769; ENSMUSG00000003051. [Q3UPW2-2]
GeneIDi13710.
KEGGimmu:13710.
UCSCiuc007csw.2. mouse. [Q3UPW2-2]
uc007csx.2. mouse. [Q3UPW2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016294 mRNA. Translation: AAB96585.1.
AK143135 mRNA. Translation: BAE25282.1.
BC138692 mRNA. Translation: AAI38693.1.
BC145380 mRNA. Translation: AAI45381.1.
CCDSiCCDS48372.1. [Q3UPW2-1]
CCDS78689.1. [Q3UPW2-2]
RefSeqiNP_001156603.1. NM_001163131.1. [Q3UPW2-1]
NP_031947.1. NM_007921.3. [Q3UPW2-2]
UniGeneiMm.441439.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3JTGX-ray2.20A289-391[»]
ProteinModelPortaliQ3UPW2.
SMRiQ3UPW2. Positions 70-151, 292-386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003135.

PTM databases

PhosphoSiteiQ3UPW2.

Proteomic databases

MaxQBiQ3UPW2.
PaxDbiQ3UPW2.
PRIDEiQ3UPW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003135; ENSMUSP00000003135; ENSMUSG00000003051. [Q3UPW2-1]
ENSMUST00000185752; ENSMUSP00000139769; ENSMUSG00000003051. [Q3UPW2-2]
GeneIDi13710.
KEGGimmu:13710.
UCSCiuc007csw.2. mouse. [Q3UPW2-2]
uc007csx.2. mouse. [Q3UPW2-1]

Organism-specific databases

CTDi1999.
MGIiMGI:1101781. Elf3.

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000232188.
HOVERGENiHBG074143.
InParanoidiQ3UPW2.
KOiK09429.
OMAiRDGFPDY.
OrthoDBiEOG7M0NRR.
PhylomeDBiQ3UPW2.
TreeFamiTF318679.

Miscellaneous databases

EvolutionaryTraceiQ3UPW2.
NextBioi284474.
PROiQ3UPW2.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UPW2.
CleanExiMM_ELF3.
GenevisibleiQ3UPW2. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033074. Elf3.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF13. PTHR11849:SF13. 3 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel epithelial-expressed ETS gene, ELF3: human and murine cDNA sequences, murine genomic organization, human mapping to 1q32.2 and expression in tissues and cancer."
    Tymms M.J., Ng A.Y.N., Thomas R.S., Schutte B.C., Zhou J., Eyre H.J., Sutherland G.R., Seth A., Rosenberg M., Papas T., Debouck C., Kola I.
    Oncogene 15:2449-2462(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY.
    Tissue: LungImported.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6JImported.
    Tissue: EyeImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  4. "The epithelium-specific ets transcription factor ESX is associated with mammary gland development and involution."
    Neve R., Chang C.-H., Scott G.K., Wong A., Friis R.R., Hynes N.E., Benz C.C.
    FASEB J. 12:1541-1550(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, INDUCTION.
  5. "Ets family transcription factor ESE-1 is expressed in corneal epithelial cells and is involved in their differentiation."
    Yoshida N., Yoshida S., Araie M., Handa H., Nabeshima Y.
    Mech. Dev. 97:27-34(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  6. "Inactivation of the transcription factor Elf3 in mice results in dysmorphogenesis and altered differentiation of intestinal epithelium."
    Ng A.-Y.N., Waring P., Ristevski S., Wang C., Wilson T., Pritchard M., Hertzog P., Kola I.
    Gastroenterology 122:1455-1466(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. "Activation of the murine type II transforming growth factor-beta receptor gene: up-regulation and function of the transcription factor Elf-3/Ert/Esx/Ese-1."
    Kim J.-H., Wilder P.J., Hou J., Nowling T., Rizzino A.
    J. Biol. Chem. 277:17520-17530(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-153 AND LEU-162.
  8. "The role of ETS transcription factors in transcription and development of mouse preimplantation embryos."
    Kageyama S., Liu H., Nagata M., Aoki F.
    Biochem. Biophys. Res. Commun. 344:675-679(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "Identification of multiple nuclear localization signals in murine Elf3, an ETS transcription factor."
    Do H.-J., Song H., Yang H.-M., Kim D.-K., Kim N.-H., Kim J.-H., Cha K.-Y., Chung H.-M., Kim J.-H.
    FEBS Lett. 580:1865-1871(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF 264-LYS--ARG-267; 269-ARG--LYS-272 AND 338-LYS--LYS-340.

Entry informationi

Entry nameiELF3_MOUSE
AccessioniPrimary (citable) accession number: Q3UPW2
Secondary accession number(s): B7ZNQ9, B9EI01, O35275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 11, 2005
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.