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Protein

ETS-related transcription factor Elf-3

Gene

Elf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter (By similarity). Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity (By similarity). Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway (By similarity). May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.By similarity4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi293 – 375ETSPROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

  • anatomical structure morphogenesis Source: MGI
  • blastocyst development Source: MGI
  • cell differentiation Source: GO_Central
  • epithelial cell differentiation Source: UniProtKB
  • extracellular matrix organization Source: MGI
  • inflammatory response Source: UniProtKB
  • mammary gland involution Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Inflammatory response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-3
Alternative name(s):
E74-like factor 3
Epithelial-restricted with serine box
Epithelium-restricted Ets protein ESX
Epithelium-specific Ets transcription factor 1
Short name:
ESE-1
Gene namesi
Name:Elf3Imported
Synonyms:Ert, Esx, Jen
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1101781. Elf3.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show about 30% fetal lethality at around E11.5. Approximately 70% of the mutant progeny are born and display severe alterations in tissue architecture in the small intestine. Elf3-deficient enterocytes express markedly reduced levels of TGFBR2.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi153D → A: Slight reduction in transactivation activity. 1 Publication1
Mutagenesisi162L → P: Significant loss of transactivation activity. 1 Publication1
Mutagenesisi264 – 267KRKR → AAAA: Abrogates nuclear localization activity. 1 Publication4
Mutagenesisi269 – 272RPRK → APAA: Clear and exclusive nuclear accumulation. 1 Publication4
Mutagenesisi338 – 340KKK → AAA: Abolishes nuclear localization. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876821 – 391ETS-related transcription factor Elf-3Add BLAST391

Proteomic databases

PaxDbiQ3UPW2.
PeptideAtlasiQ3UPW2.
PRIDEiQ3UPW2.

PTM databases

iPTMnetiQ3UPW2.
PhosphoSitePlusiQ3UPW2.

Expressioni

Tissue specificityi

Expressed in small intestine, colon, lung, kidney and uterus. Also expressed in the corneal epithelium and conjunctiva of the developing and adult eye. Not detected in liver, spleen, thymus, brain, heart, skeletal muscle or ovary.2 Publications

Developmental stagei

Expression increases progressively from E7 and is detectable in virgin mammary glands, then shows little if any change during pregnancy and declines to barely detectable levels after 3 days of lactation. Detected from E13.5 in conjunctiva epithelium. In cornea, a weak signal is detected at E16.5 and persists throughout the later stages of development.2 Publications

Inductioni

Expression in HC11 cells from midpregnant mouse mammary epithelium increases upon reaching lactogenic competency, and is down-regulated upon exposure to lactogenic hormones that induce milk protein (Beta-casein) expression. Up-regulated upon differentiation in corneal epithelium.2 Publications

Gene expression databases

BgeeiENSMUSG00000003051.
CleanExiMM_ELF3.
GenevisibleiQ3UPW2. MM.

Interactioni

Subunit structurei

Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003135.

Structurei

Secondary structure

1391
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi295 – 303Combined sources9
Helixi306 – 308Combined sources3
Beta strandi313 – 317Combined sources5
Turni318 – 321Combined sources4
Beta strandi322 – 327Combined sources6
Helixi328 – 338Combined sources11
Helixi346 – 354Combined sources9
Turni355 – 360Combined sources6
Beta strandi371 – 374Combined sources4
Turni383 – 385Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JTGX-ray2.20A289-391[»]
ProteinModelPortaliQ3UPW2.
SMRiQ3UPW2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3UPW2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 151PNTPROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the ETS family.Sequence analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000232188.
HOVERGENiHBG074143.
InParanoidiQ3UPW2.
KOiK09429.
OMAiRDGFPDY.
OrthoDBiEOG091G0BE6.
PhylomeDBiQ3UPW2.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033074. Elf3.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF13. PTHR11849:SF13. 3 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3UPW2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATCEISNV FSNYFNAMYS SEDPTLAPAP PTTFGTEDLV LTLNNQQMTL
60 70 80 90 100
EGPGPQTRSQ RDRTDPLAVL HLAEKASWTS ERPQFWSKTQ VLEWISYQVE
110 120 130 140 150
KNKYDASSID FSRCDMDGAT LCSCALEELR LVFGPLGDQL HAQLRDLTSN
160 170 180 190 200
SSDELSWIIE LLEKDGMSFQ ESLGDSGPFD QGSPFAQELL DDGRQASPYY
210 220 230 240 250
CSTYGPGAPS PGSSDVSTAR TATPQSSHAS DSGGSDVDLD LTESKVFPRD
260 270 280 290 300
GFPDYKKGEP KHGKRKRGRP RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR
310 320 330 340 350
DILIHPELNE GLMKWENRHE GVFKFLRSEA VAQLWGQKKK NSNMTYEKLS
360 370 380 390
RAMRYYYKRE ILERVDGRRL VYKFGKNSSG WKEEEVGESR N
Length:391
Mass (Da):44,273
Last modified:October 11, 2005 - v1
Checksum:i738DA753961622D4
GO
Isoform 21 Publication (identifier: Q3UPW2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-73: Missing.

Show »
Length:371
Mass (Da):42,060
Checksum:i042EDACC05839EE6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05243454 – 73Missing in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016294 mRNA. Translation: AAB96585.1.
AK143135 mRNA. Translation: BAE25282.1.
BC138692 mRNA. Translation: AAI38693.1.
BC145380 mRNA. Translation: AAI45381.1.
CCDSiCCDS48372.1. [Q3UPW2-1]
CCDS78689.1. [Q3UPW2-2]
RefSeqiNP_001156603.1. NM_001163131.1. [Q3UPW2-1]
NP_031947.1. NM_007921.3. [Q3UPW2-2]
UniGeneiMm.441439.

Genome annotation databases

EnsembliENSMUST00000003135; ENSMUSP00000003135; ENSMUSG00000003051. [Q3UPW2-1]
ENSMUST00000185752; ENSMUSP00000139769; ENSMUSG00000003051. [Q3UPW2-2]
GeneIDi13710.
KEGGimmu:13710.
UCSCiuc007csw.2. mouse. [Q3UPW2-2]
uc007csx.2. mouse. [Q3UPW2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016294 mRNA. Translation: AAB96585.1.
AK143135 mRNA. Translation: BAE25282.1.
BC138692 mRNA. Translation: AAI38693.1.
BC145380 mRNA. Translation: AAI45381.1.
CCDSiCCDS48372.1. [Q3UPW2-1]
CCDS78689.1. [Q3UPW2-2]
RefSeqiNP_001156603.1. NM_001163131.1. [Q3UPW2-1]
NP_031947.1. NM_007921.3. [Q3UPW2-2]
UniGeneiMm.441439.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JTGX-ray2.20A289-391[»]
ProteinModelPortaliQ3UPW2.
SMRiQ3UPW2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003135.

PTM databases

iPTMnetiQ3UPW2.
PhosphoSitePlusiQ3UPW2.

Proteomic databases

PaxDbiQ3UPW2.
PeptideAtlasiQ3UPW2.
PRIDEiQ3UPW2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003135; ENSMUSP00000003135; ENSMUSG00000003051. [Q3UPW2-1]
ENSMUST00000185752; ENSMUSP00000139769; ENSMUSG00000003051. [Q3UPW2-2]
GeneIDi13710.
KEGGimmu:13710.
UCSCiuc007csw.2. mouse. [Q3UPW2-2]
uc007csx.2. mouse. [Q3UPW2-1]

Organism-specific databases

CTDi1999.
MGIiMGI:1101781. Elf3.

Phylogenomic databases

eggNOGiKOG3804. Eukaryota.
ENOG4111K4J. LUCA.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000232188.
HOVERGENiHBG074143.
InParanoidiQ3UPW2.
KOiK09429.
OMAiRDGFPDY.
OrthoDBiEOG091G0BE6.
PhylomeDBiQ3UPW2.
TreeFamiTF318679.

Miscellaneous databases

EvolutionaryTraceiQ3UPW2.
PROiQ3UPW2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000003051.
CleanExiMM_ELF3.
GenevisibleiQ3UPW2. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR033074. Elf3.
IPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR11849:SF13. PTHR11849:SF13. 3 hits.
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELF3_MOUSE
AccessioniPrimary (citable) accession number: Q3UPW2
Secondary accession number(s): B7ZNQ9, B9EI01, O35275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 11, 2005
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.