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Protein

ETS-related transcription factor Elf-3

Gene

Elf3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. Acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter (By similarity). Also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. Represses KRT4 promoter activity (By similarity). Involved in mediating vascular inflammation. May play an important role in epithelial cell differentiation and tumorigenesis. May be a critical downstream effector of the ERBB2 signaling pathway (By similarity). May be associated with mammary gland development and involution. Plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.By similarity4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi293 – 375ETSPROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • transcription coactivator activity Source: UniProtKB

GO - Biological processi

  • anatomical structure morphogenesis Source: MGI
  • blastocyst development Source: MGI
  • cell differentiation Source: GO_Central
  • epithelial cell differentiation Source: UniProtKB
  • extracellular matrix organization Source: MGI
  • inflammatory response Source: UniProtKB
  • mammary gland involution Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription by RNA polymerase II Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding, Repressor
Biological processDifferentiation, Inflammatory response, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1912408 Pre-NOTCH Transcription and Translation

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-3
Alternative name(s):
E74-like factor 3
Epithelial-restricted with serine box
Epithelium-restricted Ets protein ESX
Epithelium-specific Ets transcription factor 1
Short name:
ESE-1
Gene namesi
Name:Elf3Imported
Synonyms:Ert, Esx, Jen
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1101781 Elf3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice show about 30% fetal lethality at around E11.5. Approximately 70% of the mutant progeny are born and display severe alterations in tissue architecture in the small intestine. Elf3-deficient enterocytes express markedly reduced levels of TGFBR2.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi153D → A: Slight reduction in transactivation activity. 1 Publication1
Mutagenesisi162L → P: Significant loss of transactivation activity. 1 Publication1
Mutagenesisi264 – 267KRKR → AAAA: Abrogates nuclear localization activity. 1 Publication4
Mutagenesisi269 – 272RPRK → APAA: Clear and exclusive nuclear accumulation. 1 Publication4
Mutagenesisi338 – 340KKK → AAA: Abolishes nuclear localization. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002876821 – 391ETS-related transcription factor Elf-3Add BLAST391

Proteomic databases

PaxDbiQ3UPW2
PeptideAtlasiQ3UPW2
PRIDEiQ3UPW2

PTM databases

iPTMnetiQ3UPW2
PhosphoSitePlusiQ3UPW2

Expressioni

Tissue specificityi

Expressed in small intestine, colon, lung, kidney and uterus. Also expressed in the corneal epithelium and conjunctiva of the developing and adult eye. Not detected in liver, spleen, thymus, brain, heart, skeletal muscle or ovary.2 Publications

Developmental stagei

Expression increases progressively from E7 and is detectable in virgin mammary glands, then shows little if any change during pregnancy and declines to barely detectable levels after 3 days of lactation. Detected from E13.5 in conjunctiva epithelium. In cornea, a weak signal is detected at E16.5 and persists throughout the later stages of development.2 Publications

Inductioni

Expression in HC11 cells from midpregnant mouse mammary epithelium increases upon reaching lactogenic competency, and is down-regulated upon exposure to lactogenic hormones that induce milk protein (Beta-casein) expression. Up-regulated upon differentiation in corneal epithelium.2 Publications

Gene expression databases

BgeeiENSMUSG00000003051
CleanExiMM_ELF3
GenevisibleiQ3UPW2 MM

Interactioni

Subunit structurei

Interacts with TBP. Interacts with CREBBP and EP300; these act as transcriptional coactivators of ELF3 and positively modulate its function. Interacts with XRCC5/KU86 and XRCC6/KU70; these inhibit the ability of ELF3 to bind DNA and negatively modulate its transcriptional activity. Associated with CLND7 and POU2F3 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000003135

Structurei

Secondary structure

1391
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi295 – 303Combined sources9
Helixi306 – 308Combined sources3
Beta strandi313 – 317Combined sources5
Turni318 – 321Combined sources4
Beta strandi322 – 327Combined sources6
Helixi328 – 338Combined sources11
Helixi346 – 354Combined sources9
Turni355 – 360Combined sources6
Beta strandi371 – 374Combined sources4
Turni383 – 385Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JTGX-ray2.20A289-391[»]
ProteinModelPortaliQ3UPW2
SMRiQ3UPW2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3UPW2

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 151PNTPROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the ETS family.Sequence analysis

Phylogenomic databases

eggNOGiKOG3804 Eukaryota
ENOG4111K4J LUCA
GeneTreeiENSGT00760000118996
HOGENOMiHOG000232188
HOVERGENiHBG074143
InParanoidiQ3UPW2
KOiK09429
OMAiRDGFPDY
OrthoDBiEOG091G0BE6
PhylomeDBiQ3UPW2
TreeFamiTF318679

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR033074 Elf3
IPR000418 Ets_dom
IPR003118 Pointed_dom
IPR013761 SAM/pointed_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11849:SF13 PTHR11849:SF13, 1 hit
PfamiView protein in Pfam
PF00178 Ets, 1 hit
PF02198 SAM_PNT, 1 hit
PRINTSiPR00454 ETSDOMAIN
SMARTiView protein in SMART
SM00413 ETS, 1 hit
SM00251 SAM_PNT, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF47769 SSF47769, 1 hit
PROSITEiView protein in PROSITE
PS50061 ETS_DOMAIN_3, 1 hit
PS51433 PNT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3UPW2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAATCEISNV FSNYFNAMYS SEDPTLAPAP PTTFGTEDLV LTLNNQQMTL
60 70 80 90 100
EGPGPQTRSQ RDRTDPLAVL HLAEKASWTS ERPQFWSKTQ VLEWISYQVE
110 120 130 140 150
KNKYDASSID FSRCDMDGAT LCSCALEELR LVFGPLGDQL HAQLRDLTSN
160 170 180 190 200
SSDELSWIIE LLEKDGMSFQ ESLGDSGPFD QGSPFAQELL DDGRQASPYY
210 220 230 240 250
CSTYGPGAPS PGSSDVSTAR TATPQSSHAS DSGGSDVDLD LTESKVFPRD
260 270 280 290 300
GFPDYKKGEP KHGKRKRGRP RKLSKEYWDC LEGKKSKHAP RGTHLWEFIR
310 320 330 340 350
DILIHPELNE GLMKWENRHE GVFKFLRSEA VAQLWGQKKK NSNMTYEKLS
360 370 380 390
RAMRYYYKRE ILERVDGRRL VYKFGKNSSG WKEEEVGESR N
Length:391
Mass (Da):44,273
Last modified:October 11, 2005 - v1
Checksum:i738DA753961622D4
GO
Isoform 21 Publication (identifier: Q3UPW2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     54-73: Missing.

Show »
Length:371
Mass (Da):42,060
Checksum:i042EDACC05839EE6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05243454 – 73Missing in isoform 2. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016294 mRNA Translation: AAB96585.1
AK143135 mRNA Translation: BAE25282.1
BC138692 mRNA Translation: AAI38693.1
BC145380 mRNA Translation: AAI45381.1
CCDSiCCDS48372.1 [Q3UPW2-1]
CCDS78689.1 [Q3UPW2-2]
RefSeqiNP_001156603.1, NM_001163131.1 [Q3UPW2-1]
NP_031947.1, NM_007921.3 [Q3UPW2-2]
UniGeneiMm.441439

Genome annotation databases

EnsembliENSMUST00000003135; ENSMUSP00000003135; ENSMUSG00000003051 [Q3UPW2-1]
ENSMUST00000185752; ENSMUSP00000139769; ENSMUSG00000003051 [Q3UPW2-2]
GeneIDi13710
KEGGimmu:13710
UCSCiuc007csw.2 mouse [Q3UPW2-2]
uc007csx.2 mouse [Q3UPW2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiELF3_MOUSE
AccessioniPrimary (citable) accession number: Q3UPW2
Secondary accession number(s): B7ZNQ9, B9EI01, O35275
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 11, 2005
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health