Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein transport protein Sec31A

Gene

Sec31a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein Sec31A
Alternative name(s):
SEC31-like protein 1
SEC31-related protein A
Gene namesi
Name:Sec31a
Synonyms:Sec31l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1916412. Sec31a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12301230Protein transport protein Sec31APRO_0000295148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei526 – 5261PhosphoserineCombined sources
Modified residuei531 – 5311PhosphoserineCombined sources
Cross-linki646 – 646Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei798 – 7981PhosphoserineBy similarity
Modified residuei1171 – 11711PhosphothreonineBy similarity
Modified residuei1173 – 11731PhosphoserineBy similarity
Cross-linki1227 – 1227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ3UPL0.
MaxQBiQ3UPL0.
PaxDbiQ3UPL0.
PeptideAtlasiQ3UPL0.
PRIDEiQ3UPL0.

PTM databases

iPTMnetiQ3UPL0.
PhosphoSiteiQ3UPL0.
SwissPalmiQ3UPL0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000035325.
CleanExiMM_SEC31A.
ExpressionAtlasiQ3UPL0. baseline and differential.
GenevisibleiQ3UPL0. MM.

Interactioni

Subunit structurei

COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13. Interacts with PDCD6 in a calcium-dependent manner. Interacts with KLHL12 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213263. 1 interaction.
IntActiQ3UPL0. 1 interaction.
MINTiMINT-1854463.
STRINGi10090.ENSMUSP00000092157.

Structurei

3D structure databases

ProteinModelPortaliQ3UPL0.
SMRiQ3UPL0. Positions 5-704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati4 – 4744WD 1Add
BLAST
Repeati64 – 11148WD 2Add
BLAST
Repeati120 – 16041WD 3Add
BLAST
Repeati166 – 20641WD 4Add
BLAST
Repeati209 – 25446WD 5Add
BLAST
Repeati258 – 29841WD 6Add
BLAST
Repeati301 – 34242WD 7Add
BLAST
Repeati397 – 42933WD 8; interaction with SEC13PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 470310Interaction with SEC13By similarityAdd
BLAST
Regioni799 – 1123325Interaction with PDCD6By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi832 – 1101270Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat SEC31 family.Curated
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0307. Eukaryota.
ENOG410XQ1D. LUCA.
GeneTreeiENSGT00390000003175.
HOGENOMiHOG000230582.
HOVERGENiHBG055626.
InParanoidiQ3UPL0.
KOiK14005.
OMAiQQYYPHV.
OrthoDBiEOG091G01VB.
PhylomeDBiQ3UPL0.
TreeFamiTF313842.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024298. ACE1_Sec16_Sec31.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12931. Sec16_C. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UPL0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLKEIDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL
60 70 80 90 100
SDPSLDMKSC ATFSSSHRYH KLIWGPHKMD SKGDVSGVLI AGGENGNIIL
110 120 130 140 150
YDPSKIIAGD KEVVIAQKDK HTGPVRALDV NIFQTNLVAS GANESEIYIW
160 170 180 190 200
DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK
210 220 230 240 250
NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV IQMWDLRFAS
260 270 280 290 300
SPLRVLENHA RGILAVAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP
310 320 330 340 350
TNTQWCFDIQ WCPRNPAVLS AASFDGRISV YSIMGGSIDG LRQKQVDKLS
360 370 380 390 400
SSFGNLDPFG TGQPLPPLQI PQQSAQHSIV LPLKKPPKWI RRPVGASFSF
410 420 430 440 450
GGKLVTFESV AVPLQQGAEQ QRRQPVFISQ VVTEKDFLNR SAQLQHAVQS
460 470 480 490 500
QGFIGYCQKK IEASQTEFEK NVWSFLKVNF EEDSRGKYLE LLGYRKEDLG
510 520 530 540 550
QKIALALNKV DGPDVALKDS DQVAQSDGEE SPAAEEQLLG ERIKEEKQEC
560 570 580 590 600
DFLPSAGGTF NISVSGDIDG LITRALLTGN FESAVDLCLH DNRMADAIIL
610 620 630 640 650
AIAGGQELLA QTQKKYFAKS QSKITRLITA VVMKNWREIV ESCDLKNWRE
660 670 680 690 700
ALAAVLTYAK PDEFSALCDL LGTRLEREGD SLLRTQACLC YICAGNVERL
710 720 730 740 750
VACWTKAQDG SSPLSLQDLI EKVVILRKAV QLTQALDTNT VGALLAEKMS
760 770 780 790 800
QYASLLAAQG SIAAALAFLP DNTNQPNIVQ LRDRLCKAQG KPVSGQESSQ
810 820 830 840 850
SPYERQPLSK GRPGPVAGHS QMPRVQTQQY YPHGENPPPP GFIMQGNVIP
860 870 880 890 900
NPAAPLPTAP GHMPSQLPPY PQPQPYQPAQ QYSFGTGGAA AYRPQQPVAP
910 920 930 940 950
PASNAYPNTP YISPVASYSG QPQMYTAQQA SSPTSSSAAS FPPPSSGASF
960 970 980 990 1000
QHGGPGAPPS SSAYALPPGT TGTPPAASEL PASQRTENQS FQDQASILEG
1010 1020 1030 1040 1050
PQNGWNDPPA LNRVPKKKKM PENFMPPVPI TSPIMNPSGD PQSQGLQQQP
1060 1070 1080 1090 1100
STPGPLSSHA SFPQQHLAGG QPFHGVQQPL AQTGMPPSFS KPNTEGAPGA
1110 1120 1130 1140 1150
PIGNTIQHVQ ALPTEKITKK PIPEEHLILK TTFEDLIQRC LSSATDPQTK
1160 1170 1180 1190 1200
RKLDDASKRL EFLYDKLREQ TLSPTIINGL HSIARSIETR NYSEGLSVHT
1210 1220 1230
HIVSTSNFSE TSAFMPVLKV VLSQASKLGV
Length:1,230
Mass (Da):133,569
Last modified:July 10, 2007 - v2
Checksum:iB6119EFDEF121A76
GO
Isoform 2 (identifier: Q3UPL0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     503-541: Missing.

Show »
Length:1,191
Mass (Da):129,564
Checksum:i06F5389DABBB32F9
GO
Isoform 3 (identifier: Q3UPL0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-923: Missing.

Note: No experimental confirmation available.
Show »
Length:307
Mass (Da):32,648
Checksum:iFD991C88AC4978E1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti820 – 8201S → P in AAH54358 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 923923Missing in isoform 3. 1 PublicationVSP_026752Add
BLAST
Alternative sequencei503 – 54139Missing in isoform 2. 1 PublicationVSP_026753Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC043942 mRNA. Translation: AAH43942.1.
BC054358 mRNA. Translation: AAH54358.1.
BC071249 mRNA. Translation: AAH71249.1.
AK134681 mRNA. Translation: BAE22239.1.
AK143455 mRNA. Translation: BAE25385.1.
CCDSiCCDS51573.1. [Q3UPL0-1]
RefSeqiNP_081245.1. NM_026969.1. [Q3UPL0-1]
XP_011247860.1. XM_011249558.1. [Q3UPL0-2]
UniGeneiMm.18634.

Genome annotation databases

EnsembliENSMUST00000094578; ENSMUSP00000092157; ENSMUSG00000035325. [Q3UPL0-1]
ENSMUST00000182886; ENSMUSP00000138213; ENSMUSG00000035325. [Q3UPL0-2]
GeneIDi69162.
KEGGimmu:69162.
UCSCiuc008yhi.1. mouse. [Q3UPL0-2]
uc008yhk.2. mouse. [Q3UPL0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC043942 mRNA. Translation: AAH43942.1.
BC054358 mRNA. Translation: AAH54358.1.
BC071249 mRNA. Translation: AAH71249.1.
AK134681 mRNA. Translation: BAE22239.1.
AK143455 mRNA. Translation: BAE25385.1.
CCDSiCCDS51573.1. [Q3UPL0-1]
RefSeqiNP_081245.1. NM_026969.1. [Q3UPL0-1]
XP_011247860.1. XM_011249558.1. [Q3UPL0-2]
UniGeneiMm.18634.

3D structure databases

ProteinModelPortaliQ3UPL0.
SMRiQ3UPL0. Positions 5-704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213263. 1 interaction.
IntActiQ3UPL0. 1 interaction.
MINTiMINT-1854463.
STRINGi10090.ENSMUSP00000092157.

PTM databases

iPTMnetiQ3UPL0.
PhosphoSiteiQ3UPL0.
SwissPalmiQ3UPL0.

Proteomic databases

EPDiQ3UPL0.
MaxQBiQ3UPL0.
PaxDbiQ3UPL0.
PeptideAtlasiQ3UPL0.
PRIDEiQ3UPL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000094578; ENSMUSP00000092157; ENSMUSG00000035325. [Q3UPL0-1]
ENSMUST00000182886; ENSMUSP00000138213; ENSMUSG00000035325. [Q3UPL0-2]
GeneIDi69162.
KEGGimmu:69162.
UCSCiuc008yhi.1. mouse. [Q3UPL0-2]
uc008yhk.2. mouse. [Q3UPL0-1]

Organism-specific databases

CTDi22872.
MGIiMGI:1916412. Sec31a.

Phylogenomic databases

eggNOGiKOG0307. Eukaryota.
ENOG410XQ1D. LUCA.
GeneTreeiENSGT00390000003175.
HOGENOMiHOG000230582.
HOVERGENiHBG055626.
InParanoidiQ3UPL0.
KOiK14005.
OMAiQQYYPHV.
OrthoDBiEOG091G01VB.
PhylomeDBiQ3UPL0.
TreeFamiTF313842.

Enzyme and pathway databases

ReactomeiR-MMU-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

ChiTaRSiSec31a. mouse.
PROiQ3UPL0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035325.
CleanExiMM_SEC31A.
ExpressionAtlasiQ3UPL0. baseline and differential.
GenevisibleiQ3UPL0. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024298. ACE1_Sec16_Sec31.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12931. Sec16_C. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC31A_MOUSE
AccessioniPrimary (citable) accession number: Q3UPL0
Secondary accession number(s): Q3UYH3
, Q6IQZ3, Q7TQJ7, Q811J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.