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Protein

Neutrophil elastase

Gene

Elane

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69Charge relay systemBy similarity1
Active sitei116Charge relay systemBy similarity1
Active sitei202Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • acute inflammatory response to antigenic stimulus Source: MGI
  • defense response to bacterium Source: MGI
  • defense response to fungus Source: MGI
  • leukocyte migration Source: MGI
  • leukocyte migration involved in inflammatory response Source: Ensembl
  • negative regulation of chemokine biosynthetic process Source: MGI
  • negative regulation of growth of symbiont in host Source: MGI
  • negative regulation of interleukin-8 biosynthetic process Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • neutrophil mediated killing of fungus Source: MGI
  • phagocytosis Source: MGI
  • positive regulation of immune response Source: MGI
  • positive regulation of interleukin-8 biosynthetic process Source: MGI
  • positive regulation of leukocyte tethering or rolling Source: Ensembl
  • positive regulation of smooth muscle cell proliferation Source: MGI
  • proteolysis Source: MGI
  • response to lipopolysaccharide Source: MGI
  • response to UV Source: MGI
  • response to yeast Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6803157. Antimicrobial peptides.

Protein family/group databases

MEROPSiS01.131.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil elastase (EC:3.4.21.37)
Alternative name(s):
Elastase-2
Leukocyte elastase
Gene namesi
Name:Elane
Synonyms:Ela2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2679229. Elane.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5156.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000022868627 – 265Neutrophil elastaseAdd BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 70PROSITE-ProRule annotation
Glycosylationi123N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi150 ↔ 208PROSITE-ProRule annotation
Glycosylationi172N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi180 ↔ 187PROSITE-ProRule annotation
Disulfide bondi198 ↔ 223PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ3UP87.
PaxDbiQ3UP87.
PRIDEiQ3UP87.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020125.
CleanExiMM_ELA2.
GenevisibleiQ3UP87. MM.

Interactioni

Subunit structurei

Interacts with NOTCH2NL.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206058. 1 interactor.
STRINGi10090.ENSMUSP00000038925.

Chemistry databases

BindingDBiQ3UP87.

Structurei

3D structure databases

ProteinModelPortaliQ3UP87.
SMRiQ3UP87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 247Peptidase S1PROSITE-ProRule annotationAdd BLAST219

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ3UP87.
KOiK01327.
OMAiQVFAVQR.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ3UP87.
TreeFamiTF335284.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3UP87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGRLSSRT LAAMLLALFL GGPALASEIV GGRPARPHAW PFMASLQRRG
60 70 80 90 100
GHFCGATLIA RNFVMSAAHC VNGLNFRSVQ VVLGAHDLRR QERTRQTFSV
110 120 130 140 150
QRIFENGFDP SQLLNDIVII QLNGSATINA NVQVAQLPAQ GQGVGDRTPC
160 170 180 190 200
LAMGWGRLGT NRPSPSVLQE LNVTVVTNMC RRRVNVCTLV PRRQAGICFG
210 220 230 240 250
DSGGPLVCNN LVQGIDSFIR GGCGSGLYPD AFAPVAEFAD WINSIIRSHN
260
DHLLTHPKDR EGRTN
Length:265
Mass (Da):28,648
Last modified:October 11, 2005 - v1
Checksum:i666029A299275EB6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26A → P in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti68A → V in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti102R → G in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti181R → P in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti236A → G in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti239A → V in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti248S → R in AAB60670 (PubMed:8035830).Curated1
Sequence conflicti261E → R in AAB60670 (PubMed:8035830).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04962, U06076 Genomic DNA. Translation: AAB60670.1.
AK143710 mRNA. Translation: BAE25510.1.
BC145800 mRNA. Translation: AAI45801.1.
CCDSiCCDS23994.1.
PIRiI48679.
RefSeqiNP_056594.2. NM_015779.2.
UniGeneiMm.262194.

Genome annotation databases

EnsembliENSMUST00000046091; ENSMUSP00000038925; ENSMUSG00000020125.
GeneIDi50701.
KEGGimmu:50701.
UCSCiuc007gai.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04962, U06076 Genomic DNA. Translation: AAB60670.1.
AK143710 mRNA. Translation: BAE25510.1.
BC145800 mRNA. Translation: AAI45801.1.
CCDSiCCDS23994.1.
PIRiI48679.
RefSeqiNP_056594.2. NM_015779.2.
UniGeneiMm.262194.

3D structure databases

ProteinModelPortaliQ3UP87.
SMRiQ3UP87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206058. 1 interactor.
STRINGi10090.ENSMUSP00000038925.

Chemistry databases

BindingDBiQ3UP87.
ChEMBLiCHEMBL5156.

Protein family/group databases

MEROPSiS01.131.

Proteomic databases

MaxQBiQ3UP87.
PaxDbiQ3UP87.
PRIDEiQ3UP87.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046091; ENSMUSP00000038925; ENSMUSG00000020125.
GeneIDi50701.
KEGGimmu:50701.
UCSCiuc007gai.1. mouse.

Organism-specific databases

CTDi1991.
MGIiMGI:2679229. Elane.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ3UP87.
KOiK01327.
OMAiQVFAVQR.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ3UP87.
TreeFamiTF335284.

Enzyme and pathway databases

ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiQ3UP87.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020125.
CleanExiMM_ELA2.
GenevisibleiQ3UP87. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELNE_MOUSE
AccessioniPrimary (citable) accession number: Q3UP87
Secondary accession number(s): A6H698, Q61515
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.