Reviewed,
UniProtKB/Swiss-Prot Q3UP87 (ELNE_MOUSE)
Last modified
January 19, 2010.
Version 45.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Neutrophil elastase EC=3.4.21.37 Alternative name(s): Leukocyte elastase Elastase-2 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 265 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Medullasin modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis By similarity. |
| Catalytic activity | Hydrolysis of proteins, including elastin. Preferential cleavage: Val-|-Xaa > Ala-|-Xaa. |
| Subunit structure | Interacts with NOTCH2NL By similarity. |
| Sequence similarities | Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. |
Ontologies
| Keywords | |
|---|---|
| Domain | Signal |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | leukocyte migration Inferred from mutant phenotype. Source: MGI negative regulation of growth of symbiont in hostInferred from genetic interaction. Source: MGI neutrophil mediated killing of fungusInferred from mutant phenotype. Source: MGI phagocytosisInferred from mutant phenotype. Source: MGI positive regulation of immune responseInferred from genetic interaction. Source: MGI proteolysisInferred from electronic annotation. Source: InterPro response to lipopolysaccharideInferred from genetic interaction. Source: MGI response to yeastInferred from mutant phenotype. Source: MGI |
| Molecular function | serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 26 | 26 | Potential | ||||||||
| Chain | 27 – 265 | 239 | Neutrophil elastase | PRO_0000228686 | |||||||
Regions | |||||||||||
| Domain | 29 – 247 | 219 | Peptidase S1 | ||||||||
Sites | |||||||||||
| Active site | 69 | 1 | Charge relay system By similarity | ||||||||
| Active site | 116 | 1 | Charge relay system By similarity | ||||||||
| Active site | 202 | 1 | Charge relay system By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 123 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 172 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 54 ↔ 70 | By similarity | |||||||||
| Disulfide bond | 150 ↔ 208 | By similarity | |||||||||
| Disulfide bond | 180 ↔ 187 | By similarity | |||||||||
| Disulfide bond | 198 ↔ 223 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 26 | 1 | A → P in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 68 | 1 | A → V in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 102 | 1 | R → G in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 181 | 1 | R → P in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 236 | 1 | A → G in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 239 | 1 | A → V in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 248 | 1 | S → R in AAB60670. Ref.1 | ||||||||
| Sequence conflict | 261 | 1 | E → R in AAB60670. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "PEBP2/CBF, the murine homolog of the human myeloid AML1 and PEBP2 beta/CBF beta proto-oncoproteins, regulates the murine myeloperoxidase and neutrophil elastase genes in immature myeloid cells." Nuchprayoon I., Meyers S., Scott L.M., Suzow J., Hiebert S., Friedman A.D. Mol. Cell. Biol. 14:5558-5568(1994) [PubMed: 8035830] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Spleen. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U04962, U06076 Genomic DNA. Translation: AAB60670.1. AK143710 mRNA. Translation: BAE25510.1. BC145800 mRNA. Translation: AAI45801.1. |
| IPI | IPI00122302. |
| PIR | I48679. |
| RefSeq | NP_056594.2. |
| UniGene | Mm.262194 |
3D structure databases | |
| SMR | Q3UP87. Positions 29-247. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q3UP87. |
Protein family/group databases | |
| MEROPS | S01.131. |
Proteomic databases | |
| PRIDE | Q3UP87. |
Genome annotation databases | |
| Ensembl | ENSMUST00000046091; ENSMUSP00000038925; ENSMUSG00000020125; Mus musculus. [Genome view] |
| GeneID | 50701. |
| KEGG | mmu:50701. |
| NMPDR | fig|10090.3.peg.14002. |
| UCSC | uc007gai.1. mouse. |
Organism-specific databases | |
| CTD | 50701. |
| MGI | MGI:2679229. Elane. |
Phylogenomic databases | |
| eggNOG | roNOG09047. |
| HOGENOM | HBG755338. |
| HOVERGEN | Q3UP87. |
| InParanoid | Q3UP87. |
| OMA | WINSIIR. |
| OrthoDB | EOG9CZF11. |
| PhylomeDB | Q3UP87. |
Enzyme and pathway databases | |
| BRENDA | 3.4.21.37. 244. |
Gene expression databases | |
| ArrayExpress | Q3UP87. |
| Bgee | Q3UP87. |
| CleanEx | MM_ELA2. |
| Genevestigator | Q3UP87. |
| GermOnline | ENSMUSG00000020125. Mus musculus. |
Family and domain databases | |
| InterPro | IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. IPR009003. Ser/Cys_Pept_Trypsin-like. [Graphical view] |
| Pfam | PF00089. Trypsin. 1 hit. [Graphical view] |
| PRINTS | PR00722. CHYMOTRYPSIN. |
| SMART | SM00020. Tryp_SPc. 1 hit. [Graphical view] |
| PROSITE | PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 307549. |
| SOURCE | Search... |
Entry information
| Entry name | ELNE_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q3UP87 Secondary accession number(s): A6H698, Q61515 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


