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Protein

Transcription factor CP2-like protein 1

Gene

Tfcp2l1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that facilitates establishment and maintenance of pluripotency in embryonic stem cells (ESCs) (PubMed:23942233, PubMed:26321140). With Klf2, acts as the major effector of self-renewal that mediates induction of pluripotency downstream of LIF/Stat3 and Wnt/beta-catenin signaling (PubMed:23942238, PubMed:23942233, PubMed:26321140). Required for normal duct development in the salivary gland and kidney (PubMed:17079272). Coordinates the development of the kidney collecting ducts intercalated (IC) and principal (PC) cells, which regulate acid-base and salt-water homeostasis, respectively (PubMed:28577314). Regulates the expression of IC genes including subunits B1 and D2 of the V-ATPase complex, Oxgr1, Ca12, Slc4a1, Aqp6 and IC-specific transcription factor Foxi1 (PubMed:28577314). Regulates also the expression of Jag1 and subsequent notch signaling in the collecting duct (PubMed:28577314). Jag1 initiates notch signaling in PCs but inhibits notch signaling in ICs (PubMed:28577314). Acts as a transcriptional suppressor that may suppress UBP1-mediated transcriptional activation (PubMed:11073954). Modulates the placental expression of CYP11A1 (By similarity).By similarity6 Publications

GO - Molecular functioni

GO - Biological processi

  • cell morphogenesis Source: MGI
  • cytoplasm organization Source: MGI
  • determination of adult lifespan Source: MGI
  • epithelial cell maturation Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of growth Source: MGI
  • salivary gland development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor CP2-like protein 1
Alternative name(s):
CP2-related transcriptional repressor 1
Short name:
CRTR-1
Gene namesi
Name:Tfcp2l1
Synonyms:Crtr1, Tcfcp2l1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2444691 Tfcp2l1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Approximately half of the mutant mice die before weaning and show growth retardation during early postnatal stages. Mice display defects in the morphology of the submandibular ducts, abnormalities in the maturation of renal tubules and abnormal composition of saliva and urine (PubMed:17079272). Leads to a decrease in the expression of pluripotency genes (Nanog, Oct4, Sox2 and Esrrb), while resulted in up-regulation of some endoderm (Sox17, Gata4 and Gata6), mesoderm (T and Mixl1) and trophectoderm (Cdx2, Eomes, and Elf5) markers (PubMed:28982712).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002280071 – 479Transcription factor CP2-like protein 1Add BLAST479

Proteomic databases

MaxQBiQ3UNW5
PaxDbiQ3UNW5
PeptideAtlasiQ3UNW5
PRIDEiQ3UNW5

PTM databases

iPTMnetiQ3UNW5
PhosphoSitePlusiQ3UNW5

Expressioni

Tissue specificityi

Highly expressed in placenta, testis, small intestine, kidney and stomach (PubMed:11073954, PubMed:17079272). Low levels of expression in lung, mesenteric lymph nodes, muscle, ovary, and thymus (PubMed:11073954). No expression was detected in brain, heart, liver, and spleen (PubMed:11073954). Expressed in eccrine glands in the palm (PubMed:11073954). Expression is prominent in both kidney collecting ducts intercalated (IC) and principal (PC) cells (PubMed:28577314). Also expressed in the thick limb of Henle and connecting segments of the nephron (PubMed:28577314).3 Publications

Developmental stagei

Expressed at low levels in 10.5- and 11.5-dpc embryos. Expression was not detected at 12.5- and 13.5-dpc. Highly expressed in the epithelial monolayer lining in a subset of tubules of embryonic kidney cortex. Low levels of expression were detected in 16.5-dpc embryonic intestine, limb, lung, and skin. No expression was detected in the brain. Expression is regulated in at least two distinct sites, the pluripotent cells of the developing embryo and the epithelial cells lining the embryonic kidney distal convoluted tubules. Expressed in the ducts of submandibular and sublingual glands, isolated submandibular gland, parotid and lachrymal glands and nasal gland in E16 embryos. At birth, the expression is detected in minor nasal glands in the olfactory epithelium, ducts of the mammary gland, male reproductive system, endolymphatic sac and lung. Expressed during renal development; first detected in the ureteric epithelium, at the distal end of the S-shaped body in nephron and subsequently in all nephric tubule, with expression localizing to more distal regions in the nephron during the maturation of the kidney.2 Publications

Gene expression databases

BgeeiENSMUSG00000026380
CleanExiMM_TCFCP2L1
GenevisibleiQ3UNW5 MM

Interactioni

Subunit structurei

Forms homohexamers via its SAM-like domain (By similarity). Interacts with Mta1; which is indispensable for Tfcp2l1-mediated self-renewal-promoting effect and endoderm-inhibiting action (PubMed:28982712).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
EsrrbQ615394EBI-5691372,EBI-2312731

Protein-protein interaction databases

BioGridi219895, 91 interactors
IntActiQ3UNW5, 91 interactors
STRINGi10090.ENSMUSP00000027629

Structurei

3D structure databases

ProteinModelPortaliQ3UNW5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 52Mediate transcriptional repressionAdd BLAST52
Regioni21 – 260CP2-like domainBy similarityAdd BLAST240
Regioni261 – 365SAM2-like domainBy similarityAdd BLAST105

Domaini

The CP2-like domain is required for direct DNA-binding (By similarity). The CP2-like domain is essential to maintain the undifferentiated state of embryonic stem cells (PubMed:28982712).By similarity1 Publication
The SAM-like domain is required for homohexamerization (By similarity).By similarity

Sequence similaritiesi

Belongs to the grh/CP2 family. CP2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG4091 Eukaryota
ENOG410XNZ6 LUCA
GeneTreeiENSGT00760000119235
HOGENOMiHOG000230625
HOVERGENiHBG053805
InParanoidiQ3UNW5
KOiK09275
OMAiHIILKCG
OrthoDBiEOG091G0YV2
PhylomeDBiQ3UNW5
TreeFamiTF314132

Family and domain databases

CDDicd09590 SAM_LBP9, 1 hit
InterProiView protein in InterPro
IPR007604 CP2
IPR013761 SAM/pointed_sf
IPR037598 TFCP2L1_SAM
PfamiView protein in Pfam
PF04516 CP2, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit

Sequencei

Sequence statusi: Complete.

Q3UNW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFWHTQPEH YNQHNSGSYL RDVLALPIFK QEEPQLSPEN GARLPPLQYV
60 70 80 90 100
LCAATSPAVK LHEETLTYLN QGQSYEIRLL ENRKLGDFQD LNTKYVKSII
110 120 130 140 150
RVVFHDRRLQ YTEYQQLEGW RWSRPGDRIL DIDIPLSVGI LDPRASPTQL
160 170 180 190 200
NAVEFLWDPS KRASAFIQVH CISTEFTPRK HGGEKGVPFR VQIDTFKQNE
210 220 230 240 250
SGDYSEHLHS ASCQIKVFKP KGADRKQKTD REKMEKRTAQ EKEKYQPSYE
260 270 280 290 300
TTILTECSPW PDVPYQANNT PSPSYNGSPN SFGLREGNSS PNHPVEPLPL
310 320 330 340 350
GSDHLLPSAS IQDAQQWLHR NRFSQFCWLF ASFSGADLLK MSRDDLVQVC
360 370 380 390 400
GPADGIRLFN AIKGRNVRPK MTIYVCQELE QNQLPLPQKQ DDSGDNSLCV
410 420 430 440 450
YHAIFLEELT TLELTEKIAS LYSIPPQHIH RVYRQGPAGI HVVVSNEMVQ
460 470
NFQDESCFIL STLKAESNDG YHIILKCGL
Length:479
Mass (Da):54,737
Last modified:July 11, 2012 - v2
Checksum:i4765C41F5ECCB8C6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti60K → R in AAK01150 (PubMed:11073954).Curated1
Sequence conflicti195T → A in AAK01150 (PubMed:11073954).Curated1
Sequence conflicti201 – 212SGDYS…LHSAS → KWGLLGASTLLPA in AAK01150 (PubMed:11073954).CuratedAdd BLAST12
Sequence conflicti217V → G in AAK01150 (PubMed:11073954).Curated1
Sequence conflicti220 – 227PKGADRKQ → AQGELIGNK in AAK01150 (PubMed:11073954).Curated8
Sequence conflicti269 – 270NT → TP in AAK01150 (PubMed:11073954).Curated2
Sequence conflicti288N → T in AAK01150 (PubMed:11073954).Curated1
Sequence conflicti292N → I in AAK01150 (PubMed:11073954).Curated1
Sequence conflicti465Missing in BAE25632 (PubMed:16141072).Curated1
Sequence conflicti468N → S in AAK01150 (PubMed:11073954).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF311309 mRNA Translation: AAK01150.1
AK143960 mRNA Translation: BAE25632.1
AC109271 Genomic DNA No translation available.
AC109294 Genomic DNA No translation available.
CH466520 Genomic DNA Translation: EDL39825.1
BC116663 mRNA Translation: AAI16664.1
CCDSiCCDS35691.1
RefSeqiNP_076244.2, NM_023755.2
UniGeneiMm.24621

Genome annotation databases

EnsembliENSMUST00000027629; ENSMUSP00000027629; ENSMUSG00000026380
GeneIDi81879
KEGGimmu:81879
UCSCiuc007cil.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTF2L1_MOUSE
AccessioniPrimary (citable) accession number: Q3UNW5
Secondary accession number(s): Q14AW3, Q99PF2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: July 11, 2012
Last modified: May 23, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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