Q3UNH4 (GRIN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
April 3, 2013.
Version 59.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: G protein-regulated inducer of neurite outgrowth 1 Short name=GRIN1 | ||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 932 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May be involved in neurite outgrowth. Ref.3 |
| Subunit structure | Interacts with activated forms of GNAI1, GNAO1 and GNAZ. Ref.3 Ref.5 |
| Subcellular location | Cell membrane; Lipid-anchor. Cell projection › growth cone By similarity. Note: Highly enriched in growth cone. Ref.3 Ref.5 |
| Tissue specificity | Expressed specifically in brain (at protein level). Ref.3 |
| Post-translational modification | Palmitoylation on Cys-923 and/or Cys-924 is required for membrane targeting. Ref.5 |
| Sequence caution | The sequence AAD55371.1 differs from that shown. Reason: Erroneous initiation. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Cell projection Membrane |
| PTM | Lipoprotein Palmitate Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | neuron projection development Inferred from direct assay Ref.3. Source: MGI |
| Cellular_component | growth cone Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from direct assay Ref.3. Source: MGI |
| Molecular_function | phosphoprotein binding Inferred from direct assay Ref.3. Source: MGI |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 932 | 932 | G protein-regulated inducer of neurite outgrowth 1 | PRO_0000235978 | |||||
Regions | |||||||||
| Region | 821 – 932 | 112 | Interaction with GNAO1 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 182 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 190 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 495 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 575 | 1 | Phosphothreonine Ref.7 | ||||||
| Modified residue | 691 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 693 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 714 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 795 | 1 | Phosphothreonine Ref.6 Ref.7 | ||||||
| Lipidation | 923 | 1 | S-palmitoyl cysteine Probable | ||||||
| Lipidation | 924 | 1 | S-palmitoyl cysteine Probable | ||||||
Experimental info | |||||||||
| Mutagenesis | 923 – 924 | 2 | CC → AA: Abolishes membrane targeting. Ref.5 | ||||||
| Sequence conflict | 215 | 1 | E → G in AAD55371. Ref.3 | ||||||
| Sequence conflict | 458 | 1 | K → R in BAE25773. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.  Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain. |
| [3] | "A candidate target for G protein action in brain." Chen L.T., Gilman A.G., Kozasa T. J. Biol. Chem. 274:26931-26938(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 92-932, TISSUE SPECIFICITY, INTERACTION WITH GNAI1; GNAO1 AND GNAZ, SUBCELLULAR LOCATION, FUNCTION. Tissue: Brain. |
| [4] | Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 312-325; 774-787 AND 813-833, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus. |
| [5] | "Functional characterization of Galphao signaling through G protein-regulated inducer of neurite outgrowth 1." Nakata H., Kozasa T. Mol. Pharmacol. 67:695-702(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GNAO1, SUBCELLULAR LOCATION, MUTAGENESIS OF 923-CYS-CYS-924, PALMITOYLATION AT CYS-923 AND CYS-924. |
| [6] | "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-795, MASS SPECTROMETRY. Tissue: Brain. |
| [7] | "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182; SER-190; SER-495; THR-575; SER-691; SER-693; SER-714 AND THR-795, MASS SPECTROMETRY. Tissue: Brain cortex. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK138306 mRNA. Translation: BAE23617.1. AK144210 mRNA. Translation: BAE25773.1. BC057044 mRNA. Translation: AAH57044.1. AF146569 mRNA. Translation: AAD55371.1. Different initiation. |
| IPI | IPI00138232. |
| RefSeq | NP_036144.2. NM_012014.3. |
| UniGene | Mm.41812. |
3D structure databases | |
| ProteinModelPortal | Q3UNH4. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q3UNH4. 1 interaction. |
PTM databases | |
| PhosphoSite | Q3UNH4. |
Proteomic databases | |
| PaxDb | Q3UNH4. |
| PRIDE | Q3UNH4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000099506; ENSMUSP00000097106; ENSMUSG00000069227. ENSMUST00000135343; ENSMUSP00000115539; ENSMUSG00000069227. |
| GeneID | 26913. |
| KEGG | mmu:26913. |
| UCSC | uc007qpa.1. mouse. |
Organism-specific databases | |
| CTD | 114787. |
| MGI | MGI:1349455. Gprin1. |
Phylogenomic databases | |
| eggNOG | NOG12793. |
| GeneTree | ENSGT00570000079168. |
| HOGENOM | HOG000112813. |
| HOVERGEN | HBG081574. |
| InParanoid | Q3UNH4. |
| OMA | REDAGTQ. |
Gene expression databases | |
| Bgee | Q3UNH4. |
| Genevestigator | Q3UNH4. |
| GermOnline | ENSMUSG00000069227. Mus musculus. |
Family and domain databases | |
| ProtoNet | Search... |
Other | |
| NextBio | 304789. |
| SOURCE | Search... |
Entry information
| Entry name | GRIN1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q3UNH4 Secondary accession number(s): Q6PGF9, Q9QZY2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |