Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Src kinase-associated phosphoprotein 2

Gene

Skap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway.3 Publications

GO - Molecular functioni

GO - Biological processi

  • B cell activation Source: UniProtKB-KW
  • immune response-activating signal transduction Source: GO_Central
  • negative regulation of cell proliferation Source: MGI
Complete GO annotation...

Keywords - Biological processi

B-cell activation

Enzyme and pathway databases

ReactomeiR-MMU-391160. Signal regulatory protein (SIRP) family interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Src kinase-associated phosphoprotein 2
Alternative name(s):
Pyk2/RAFTK-associated protein
SKAP55 homolog
Short name:
SKAP-HOM
Src family-associated phosphoprotein 2
Src kinase-associated phosphoprotein 55-related protein
Src-associated adapter protein with PH and SH3 domains
Gene namesi
Name:Skap2
Synonyms:Prap, Ra70, Saps, Scap2, Skap55r
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1889206. Skap2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are healthy and do not display any obvious abnormality. They have normal T-cell, platelet and macrophage function, but show reduced levels of spontaneous immunoglobulins in the serum, and defects in B-cell proliferation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi260Y → F: Abolishes interaction with FYN, phosphorylation by FYN, and effects on cell growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002701801 – 358Src kinase-associated phosphoprotein 2Add BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5PhosphoserineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei75PhosphotyrosineCombined sources1
Modified residuei87PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei151PhosphotyrosineCombined sources1
Modified residuei197PhosphotyrosineBy similarity1
Modified residuei223PhosphoserineBy similarity1
Modified residuei260Phosphotyrosine; by FYNCombined sources1 Publication1
Modified residuei272PhosphoserineBy similarity1
Modified residuei282PhosphoserineBy similarity1
Modified residuei285PhosphoserineBy similarity1

Post-translational modificationi

Dephosphorylated on Tyr-75 by PTPN22 (By similarity). Phosphorylated by FYN on Tyr-260. In case of infection with Y.pseudotuberculosis, dephosphorylated by bacterial phosphatase yopH.By similarity3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ3UND0.
PaxDbiQ3UND0.
PeptideAtlasiQ3UND0.
PRIDEiQ3UND0.

PTM databases

iPTMnetiQ3UND0.
PhosphoSitePlusiQ3UND0.

Expressioni

Tissue specificityi

Expressed in kidney, lung, liver, spleen, bone marrow and testis. Present in T-cells, B-cells, and all cells of the myelomonocytic lineage. Present in all brain regions, with highest levels in neurons from the Purkinje cell layer, hippocampal gyrus, cortex and substantia nigra (at protein level).4 Publications

Inductioni

By IL-6 in myeloid cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000059182.
CleanExiMM_SKAP2.
ExpressionAtlasiQ3UND0. baseline and differential.
GenevisibleiQ3UND0. MM.

Interactioni

Subunit structurei

Interacts with LAT, GRB2, PTK2B and PRAM1 (By similarity). Homodimer. Interacts with FYB, which is required for SKAP2 protein stability. Interacts with PTPNS1. Part of a complex consisting of SKAP2, FYB and PTPNS1. Part of a complex consisting of SKAP2, FYB and LILRB3. May interact with actin. May interact with FYN, HCK and LYN. Interacts with FASLG (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PTPN22Q9Y2R22EBI-642769,EBI-1211241From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207619. 1 interactor.
IntActiQ3UND0. 2 interactors.
MINTiMINT-263820.
STRINGi10090.ENSMUSP00000077342.

Structurei

Secondary structure

1358
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 29Combined sources15
Turni30 – 34Combined sources5
Helixi39 – 55Combined sources17
Helixi56 – 59Combined sources4
Helixi61 – 63Combined sources3
Helixi111 – 113Combined sources3
Beta strandi115 – 126Combined sources12
Beta strandi128 – 130Combined sources3
Turni132 – 134Combined sources3
Beta strandi136 – 145Combined sources10
Beta strandi148 – 154Combined sources7
Beta strandi161 – 165Combined sources5
Beta strandi170 – 173Combined sources4
Helixi175 – 177Combined sources3
Helixi183 – 185Combined sources3
Beta strandi186 – 190Combined sources5
Beta strandi192 – 194Combined sources3
Beta strandi196 – 200Combined sources5
Helixi204 – 217Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0VNMR-B73-79[»]
1U5EX-ray2.60A/B14-222[»]
1U5FX-ray1.90A111-248[»]
1U5GX-ray2.10A/B/C/D103-222[»]
2OTXX-ray2.60A/B12-222[»]
ProteinModelPortaliQ3UND0.
SMRiQ3UND0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ3UND0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini116 – 219PHPROSITE-ProRule annotationAdd BLAST104
Domaini296 – 357SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 64HomodimerizationAdd BLAST51

Domaini

The SH3 domain interacts with FYB and PTK2B.By similarity

Sequence similaritiesi

Belongs to the SKAP family.Curated
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IH8Q. Eukaryota.
ENOG410ZVZV. LUCA.
GeneTreeiENSGT00390000017856.
HOGENOMiHOG000231109.
HOVERGENiHBG052827.
InParanoidiQ3UND0.
OMAiCFEICAP.
OrthoDBiEOG091G0HBY.
PhylomeDBiQ3UND0.
TreeFamiTF331055.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UND0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPNPSCTSSP GPLPEEIRNL LADVETFVAD TLKGENLSKK AKEKRESLIK
60 70 80 90 100
KIKDVKSVYL QEFQDKGDAE DGDEYDDPFA GPADTISLAS ERYDKDDDGP
110 120 130 140 150
SDGNQFPPIA AQDLPFVIKA GYLEKRRKDH SFLGFEWQKR WCALSKTVFY
160 170 180 190 200
YYGSDKDKQQ KGEFAIDGYD VRMNNTLRKD GKKDCCFEIC APDKRIYQFT
210 220 230 240 250
AASPKDAEEW VQQLKFILQD LGSDVIPEDD EERGELYDDV DHPAAVSSPQ
260 270 280 290 300
RSQPIDDEIY EELPEEEEDT ASVKMDEQGK GSRDSVHHTS GDKSTDYANF
310 320 330 340 350
YQGLWDCTGA LSDELSFKRG DVIYILSKEY NRYGWWVGEM KGAIGLVPKA

YLMEMYDI
Length:358
Mass (Da):40,712
Last modified:January 9, 2007 - v2
Checksum:iB66973A656E44E05
GO
Isoform 2 (identifier: Q3UND0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-28: Missing.

Note: No experimental confirmation available.
Show »
Length:351
Mass (Da):39,950
Checksum:iA6C08305272498D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16E → K in BAE25817 (PubMed:16141072).Curated1
Sequence conflicti104N → P AA sequence (PubMed:11207596).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02218422 – 28Missing in isoform 2. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051324 mRNA. Translation: AAC99297.1.
AB014485 mRNA. Translation: BAA77253.1.
AK076000 mRNA. Translation: BAC36111.1.
AK144289 mRNA. Translation: BAE25817.1.
BC003711 mRNA. Translation: AAH03711.1.
CCDSiCCDS20137.1. [Q3UND0-1]
RefSeqiNP_061243.1. NM_018773.2. [Q3UND0-1]
UniGeneiMm.221479.
Mm.392558.

Genome annotation databases

EnsembliENSMUST00000078214; ENSMUSP00000077342; ENSMUSG00000059182. [Q3UND0-2]
ENSMUST00000204778; ENSMUSP00000145462; ENSMUSG00000059182. [Q3UND0-1]
GeneIDi54353.
KEGGimmu:54353.
UCSCiuc009bxv.1. mouse. [Q3UND0-1]
uc009bxw.1. mouse. [Q3UND0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051324 mRNA. Translation: AAC99297.1.
AB014485 mRNA. Translation: BAA77253.1.
AK076000 mRNA. Translation: BAC36111.1.
AK144289 mRNA. Translation: BAE25817.1.
BC003711 mRNA. Translation: AAH03711.1.
CCDSiCCDS20137.1. [Q3UND0-1]
RefSeqiNP_061243.1. NM_018773.2. [Q3UND0-1]
UniGeneiMm.221479.
Mm.392558.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0VNMR-B73-79[»]
1U5EX-ray2.60A/B14-222[»]
1U5FX-ray1.90A111-248[»]
1U5GX-ray2.10A/B/C/D103-222[»]
2OTXX-ray2.60A/B12-222[»]
ProteinModelPortaliQ3UND0.
SMRiQ3UND0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207619. 1 interactor.
IntActiQ3UND0. 2 interactors.
MINTiMINT-263820.
STRINGi10090.ENSMUSP00000077342.

PTM databases

iPTMnetiQ3UND0.
PhosphoSitePlusiQ3UND0.

Proteomic databases

MaxQBiQ3UND0.
PaxDbiQ3UND0.
PeptideAtlasiQ3UND0.
PRIDEiQ3UND0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078214; ENSMUSP00000077342; ENSMUSG00000059182. [Q3UND0-2]
ENSMUST00000204778; ENSMUSP00000145462; ENSMUSG00000059182. [Q3UND0-1]
GeneIDi54353.
KEGGimmu:54353.
UCSCiuc009bxv.1. mouse. [Q3UND0-1]
uc009bxw.1. mouse. [Q3UND0-2]

Organism-specific databases

CTDi8935.
MGIiMGI:1889206. Skap2.

Phylogenomic databases

eggNOGiENOG410IH8Q. Eukaryota.
ENOG410ZVZV. LUCA.
GeneTreeiENSGT00390000017856.
HOGENOMiHOG000231109.
HOVERGENiHBG052827.
InParanoidiQ3UND0.
OMAiCFEICAP.
OrthoDBiEOG091G0HBY.
PhylomeDBiQ3UND0.
TreeFamiTF331055.

Enzyme and pathway databases

ReactomeiR-MMU-391160. Signal regulatory protein (SIRP) family interactions.

Miscellaneous databases

EvolutionaryTraceiQ3UND0.
PROiQ3UND0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000059182.
CleanExiMM_SKAP2.
ExpressionAtlasiQ3UND0. baseline and differential.
GenevisibleiQ3UND0. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSKAP2_MOUSE
AccessioniPrimary (citable) accession number: Q3UND0
Secondary accession number(s): Q8BK74, Q9Z2K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.