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Q3UN04

- UBP30_MOUSE

UniProt

Q3UN04 - UBP30_MOUSE

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Protein

Ubiquitin carboxyl-terminal hydrolase 30

Gene
Usp30
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy By similarity. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Enzyme regulationi

Inhibited by the diterpenoid derivative 15-oxospiramilactone (S3).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771Nucleophile
Active sitei452 – 4521Proton acceptor By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 30 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 30
Ubiquitin thioesterase 30
Ubiquitin-specific-processing protease 30
Short name:
Ub-specific protease 30
Gene namesi
Name:Usp30
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:2140991. Usp30.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535Mitochondrial intermembrane Reviewed predictionAdd
BLAST
Transmembranei36 – 5621Helical; Reviewed predictionAdd
BLAST
Topological domaini57 – 517461Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial outer membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771C → S: Loss of deubiquitinase activity and binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor.
Mutagenesisi234 – 2341C → S: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor.
Mutagenesisi284 – 2841C → S: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor.
Mutagenesisi452 – 4521H → A: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Ubiquitin carboxyl-terminal hydrolase 30PRO_0000377537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki235 – 235Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity
Cross-linki289 – 289Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Post-translational modificationi

Ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289, leading to its degradation By similarity.

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ3UN04.
PRIDEiQ3UN04.

Expressioni

Gene expression databases

BgeeiQ3UN04.
GenevestigatoriQ3UN04.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031588.

Structurei

3D structure databases

ProteinModelPortaliQ3UN04.
SMRiQ3UN04. Positions 69-331, 415-501.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 502435USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 USP domain.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG259866.
GeneTreeiENSGT00550000075075.
HOGENOMiHOG000065744.
InParanoidiQ8BVI3.
KOiK11851.
OMAiNQPGGPK.
OrthoDBiEOG7P8P7N.
PhylomeDBiQ3UN04.
TreeFamiTF105781.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UN04-1 [UniParc]FASTAAdd to Basket

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MLSSRAQAAR TAADKALQRF LRTGAAVRYK VMKNWGVIGG IAAALAAGIY    50
VIWGPITERK KRRKGLVPGL VNLGNTCFMN SLLQGLSACP AFVKWLEEFT 100
TQYSRDQQGP HTHQCLSLTL LNLLKALSCQ EVTEDEVLDA SCLLDVLRMY 150
RWQISSFEEQ DAHELFHVIT SSLEDERDRQ PRVTHLFDVH SLEQQSEMAP 200
RQVTCHTRGS PHPTTNHWKS QHPFHGRLTS NMVCKHCEHQ SPVRFDTFDS 250
LSLSIPAATW GHPLTLDHCL HHFISSESVR DVVCDNCTKI EARGTLTGEK 300
VEHQRSTFVK QLKLGKLPQC LCIHLQRLSW SSHGTPLKRH EHVQFNEFLM 350
MDFYKYRLLG HKPSQHGPKA TENPGSAPEV QDAQAAPKPG LSQPGAPKTQ 400
IFLNGACSPS LLPALPSPVA FPLPVVPDYS SSTYLFRLMA VVVHHGDMHS 450
GHFVTYRRSP PSAKNPLSTS NQWLWISDDT VRKASLQEVL SSSAYLLFYE 500
RVLSRVQQQG REYRSEE 517
Length:517
Mass (Da):58,221
Last modified:October 11, 2005 - v1
Checksum:i561866EF23103048
GO

Sequence cautioni

The sequence AAH38606.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK078164 mRNA. Translation: BAC37154.1.
AK144578 mRNA. Translation: BAE25944.1.
AK162153 mRNA. Translation: BAE36756.1.
AK162269 mRNA. Translation: BAE36827.1.
BC038606 mRNA. Translation: AAH38606.1. Different initiation.
CCDSiCCDS19558.1.
RefSeqiNP_001028374.1. NM_001033202.3.
UniGeneiMm.240421.

Genome annotation databases

EnsembliENSMUST00000031588; ENSMUSP00000031588; ENSMUSG00000029592.
GeneIDi100756.
KEGGimmu:100756.
UCSCiuc008yzc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK078164 mRNA. Translation: BAC37154.1 .
AK144578 mRNA. Translation: BAE25944.1 .
AK162153 mRNA. Translation: BAE36756.1 .
AK162269 mRNA. Translation: BAE36827.1 .
BC038606 mRNA. Translation: AAH38606.1 . Different initiation.
CCDSi CCDS19558.1.
RefSeqi NP_001028374.1. NM_001033202.3.
UniGenei Mm.240421.

3D structure databases

ProteinModelPortali Q3UN04.
SMRi Q3UN04. Positions 69-331, 415-501.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000031588.

Protein family/group databases

MEROPSi C19.060.

Proteomic databases

PaxDbi Q3UN04.
PRIDEi Q3UN04.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000031588 ; ENSMUSP00000031588 ; ENSMUSG00000029592 .
GeneIDi 100756.
KEGGi mmu:100756.
UCSCi uc008yzc.1. mouse.

Organism-specific databases

CTDi 84749.
MGIi MGI:2140991. Usp30.

Phylogenomic databases

eggNOGi NOG259866.
GeneTreei ENSGT00550000075075.
HOGENOMi HOG000065744.
InParanoidi Q8BVI3.
KOi K11851.
OMAi NQPGGPK.
OrthoDBi EOG7P8P7N.
PhylomeDBi Q3UN04.
TreeFami TF105781.

Miscellaneous databases

NextBioi 354628.
PROi Q3UN04.
SOURCEi Search...

Gene expression databases

Bgeei Q3UN04.
Genevestigatori Q3UN04.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Lung and Medulla oblongata.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  3. "A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30."
    Yue W., Chen Z., Liu H., Yan C., Chen M., Feng D., Yan C., Wu H., Du L., Wang Y., Liu J., Huang X., Xia L., Liu L., Wang X., Jin H., Wang J., Song Z., Hao X., Chen Q.
    Cell Res. 24:482-496(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, ACTIVE SITE, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-77; CYS-234; CYS-284 AND HIS-452.

Entry informationi

Entry nameiUBP30_MOUSE
AccessioniPrimary (citable) accession number: Q3UN04
Secondary accession number(s): Q3TS48
, Q3TSB9, Q8BVI3, Q8CHW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 11, 2005
Last modified: September 3, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi