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Q3UN04

- UBP30_MOUSE

UniProt

Q3UN04 - UBP30_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 30

Gene

Usp30

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (11 Oct 2005)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy By similarity. Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.By similarity1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Enzyme regulationi

    Inhibited by the diterpenoid derivative 15-oxospiramilactone (S3).1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei77 – 771Nucleophile1 PublicationPROSITE-ProRule annotation
    Active sitei452 – 4521Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. ubiquitin-specific protease activity Source: UniProtKB
    4. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. mitochondrial fusion Source: UniProtKB
    2. mitochondrion degradation Source: UniProtKB
    3. protein deubiquitination Source: UniProtKB
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.060.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 30 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 30
    Ubiquitin thioesterase 30
    Ubiquitin-specific-processing protease 30
    Short name:
    Ub-specific protease 30
    Gene namesi
    Name:Usp30
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:2140991. Usp30.

    Subcellular locationi

    Mitochondrion outer membrane 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial outer membrane Source: UniProtKB
    3. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi77 – 771C → S: Loss of deubiquitinase activity and binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication
    Mutagenesisi234 – 2341C → S: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication
    Mutagenesisi284 – 2841C → S: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication
    Mutagenesisi452 – 4521H → A: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 517517Ubiquitin carboxyl-terminal hydrolase 30PRO_0000377537Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki235 – 235Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Cross-linki289 – 289Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

    Post-translational modificationi

    Ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289, leading to its degradation.By similarity

    Keywords - PTMi

    Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiQ3UN04.
    PRIDEiQ3UN04.

    Expressioni

    Gene expression databases

    BgeeiQ3UN04.
    GenevestigatoriQ3UN04.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000031588.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3UN04.
    SMRiQ3UN04. Positions 69-331, 415-501.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3535Mitochondrial intermembraneSequence AnalysisAdd
    BLAST
    Topological domaini57 – 517461CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini68 – 502435USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG259866.
    GeneTreeiENSGT00550000075075.
    HOGENOMiHOG000065744.
    InParanoidiQ8BVI3.
    KOiK11851.
    OMAiNQPGGPK.
    OrthoDBiEOG7P8P7N.
    PhylomeDBiQ3UN04.
    TreeFamiTF105781.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3UN04-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLSSRAQAAR TAADKALQRF LRTGAAVRYK VMKNWGVIGG IAAALAAGIY    50
    VIWGPITERK KRRKGLVPGL VNLGNTCFMN SLLQGLSACP AFVKWLEEFT 100
    TQYSRDQQGP HTHQCLSLTL LNLLKALSCQ EVTEDEVLDA SCLLDVLRMY 150
    RWQISSFEEQ DAHELFHVIT SSLEDERDRQ PRVTHLFDVH SLEQQSEMAP 200
    RQVTCHTRGS PHPTTNHWKS QHPFHGRLTS NMVCKHCEHQ SPVRFDTFDS 250
    LSLSIPAATW GHPLTLDHCL HHFISSESVR DVVCDNCTKI EARGTLTGEK 300
    VEHQRSTFVK QLKLGKLPQC LCIHLQRLSW SSHGTPLKRH EHVQFNEFLM 350
    MDFYKYRLLG HKPSQHGPKA TENPGSAPEV QDAQAAPKPG LSQPGAPKTQ 400
    IFLNGACSPS LLPALPSPVA FPLPVVPDYS SSTYLFRLMA VVVHHGDMHS 450
    GHFVTYRRSP PSAKNPLSTS NQWLWISDDT VRKASLQEVL SSSAYLLFYE 500
    RVLSRVQQQG REYRSEE 517
    Length:517
    Mass (Da):58,221
    Last modified:October 11, 2005 - v1
    Checksum:i561866EF23103048
    GO

    Sequence cautioni

    The sequence AAH38606.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK078164 mRNA. Translation: BAC37154.1.
    AK144578 mRNA. Translation: BAE25944.1.
    AK162153 mRNA. Translation: BAE36756.1.
    AK162269 mRNA. Translation: BAE36827.1.
    BC038606 mRNA. Translation: AAH38606.1. Different initiation.
    CCDSiCCDS19558.1.
    RefSeqiNP_001028374.1. NM_001033202.3.
    UniGeneiMm.240421.

    Genome annotation databases

    EnsembliENSMUST00000031588; ENSMUSP00000031588; ENSMUSG00000029592.
    GeneIDi100756.
    KEGGimmu:100756.
    UCSCiuc008yzc.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK078164 mRNA. Translation: BAC37154.1 .
    AK144578 mRNA. Translation: BAE25944.1 .
    AK162153 mRNA. Translation: BAE36756.1 .
    AK162269 mRNA. Translation: BAE36827.1 .
    BC038606 mRNA. Translation: AAH38606.1 . Different initiation.
    CCDSi CCDS19558.1.
    RefSeqi NP_001028374.1. NM_001033202.3.
    UniGenei Mm.240421.

    3D structure databases

    ProteinModelPortali Q3UN04.
    SMRi Q3UN04. Positions 69-331, 415-501.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000031588.

    Protein family/group databases

    MEROPSi C19.060.

    Proteomic databases

    PaxDbi Q3UN04.
    PRIDEi Q3UN04.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031588 ; ENSMUSP00000031588 ; ENSMUSG00000029592 .
    GeneIDi 100756.
    KEGGi mmu:100756.
    UCSCi uc008yzc.1. mouse.

    Organism-specific databases

    CTDi 84749.
    MGIi MGI:2140991. Usp30.

    Phylogenomic databases

    eggNOGi NOG259866.
    GeneTreei ENSGT00550000075075.
    HOGENOMi HOG000065744.
    InParanoidi Q8BVI3.
    KOi K11851.
    OMAi NQPGGPK.
    OrthoDBi EOG7P8P7N.
    PhylomeDBi Q3UN04.
    TreeFami TF105781.

    Miscellaneous databases

    NextBioi 354628.
    PROi Q3UN04.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q3UN04.
    Genevestigatori Q3UN04.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo, Lung and Medulla oblongata.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    3. "A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30."
      Yue W., Chen Z., Liu H., Yan C., Chen M., Feng D., Yan C., Wu H., Du L., Wang Y., Liu J., Huang X., Xia L., Liu L., Wang X., Jin H., Wang J., Song Z., Hao X., Chen Q.
      Cell Res. 24:482-496(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, ACTIVE SITE, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-77; CYS-234; CYS-284 AND HIS-452.

    Entry informationi

    Entry nameiUBP30_MOUSE
    AccessioniPrimary (citable) accession number: Q3UN04
    Secondary accession number(s): Q3TS48
    , Q3TSB9, Q8BVI3, Q8CHW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: October 11, 2005
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3