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Protein

Ubiquitin carboxyl-terminal hydrolase 30

Gene

Usp30

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PARK2): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate to mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65' (By similarity). Acts as negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (PubMed:24513856).By similarity1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Enzyme regulationi

Inhibited by the diterpenoid derivative 15-oxospiramilactone (S3).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei77 – 771NucleophilePROSITE-ProRule annotation1 Publication
Active sitei452 – 4521Proton acceptorPROSITE-ProRule annotation1 Publication

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: UniProtKB
  • ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 30 (EC:3.4.19.12By similarity)
Alternative name(s):
Deubiquitinating enzyme 30
Ubiquitin thioesterase 30
Ubiquitin-specific-processing protease 30
Short name:
Ub-specific protease 30
Gene namesi
Name:Usp30
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2140991. Usp30.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3535Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei36 – 5621HelicalSequence AnalysisAdd
BLAST
Topological domaini57 – 517461CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial outer membrane Source: UniProtKB
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771C → S: Loss of deubiquitinase activity and binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication
Mutagenesisi234 – 2341C → S: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication
Mutagenesisi284 – 2841C → S: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication
Mutagenesisi452 – 4521H → A: Does not affect binding to diterpenoid derivative 15-oxospiramilactone (S3) inhibitor. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Ubiquitin carboxyl-terminal hydrolase 30PRO_0000377537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki235 – 235Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki289 – 289Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289, leading to its degradation.By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiQ3UN04.
PaxDbiQ3UN04.
PRIDEiQ3UN04.

Expressioni

Gene expression databases

BgeeiQ3UN04.
GenevisibleiQ3UN04. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031588.

Structurei

3D structure databases

ProteinModelPortaliQ3UN04.
SMRiQ3UN04. Positions 69-331, 415-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 502435USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG259866.
GeneTreeiENSGT00550000075075.
HOGENOMiHOG000065744.
InParanoidiQ3UN04.
KOiK11851.
OMAiMKNWGVL.
OrthoDBiEOG7P8P7N.
PhylomeDBiQ3UN04.
TreeFamiTF105781.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UN04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSSRAQAAR TAADKALQRF LRTGAAVRYK VMKNWGVIGG IAAALAAGIY
60 70 80 90 100
VIWGPITERK KRRKGLVPGL VNLGNTCFMN SLLQGLSACP AFVKWLEEFT
110 120 130 140 150
TQYSRDQQGP HTHQCLSLTL LNLLKALSCQ EVTEDEVLDA SCLLDVLRMY
160 170 180 190 200
RWQISSFEEQ DAHELFHVIT SSLEDERDRQ PRVTHLFDVH SLEQQSEMAP
210 220 230 240 250
RQVTCHTRGS PHPTTNHWKS QHPFHGRLTS NMVCKHCEHQ SPVRFDTFDS
260 270 280 290 300
LSLSIPAATW GHPLTLDHCL HHFISSESVR DVVCDNCTKI EARGTLTGEK
310 320 330 340 350
VEHQRSTFVK QLKLGKLPQC LCIHLQRLSW SSHGTPLKRH EHVQFNEFLM
360 370 380 390 400
MDFYKYRLLG HKPSQHGPKA TENPGSAPEV QDAQAAPKPG LSQPGAPKTQ
410 420 430 440 450
IFLNGACSPS LLPALPSPVA FPLPVVPDYS SSTYLFRLMA VVVHHGDMHS
460 470 480 490 500
GHFVTYRRSP PSAKNPLSTS NQWLWISDDT VRKASLQEVL SSSAYLLFYE
510
RVLSRVQQQG REYRSEE
Length:517
Mass (Da):58,221
Last modified:October 11, 2005 - v1
Checksum:i561866EF23103048
GO

Sequence cautioni

The sequence AAH38606.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078164 mRNA. Translation: BAC37154.1.
AK144578 mRNA. Translation: BAE25944.1.
AK162153 mRNA. Translation: BAE36756.1.
AK162269 mRNA. Translation: BAE36827.1.
BC038606 mRNA. Translation: AAH38606.1. Different initiation.
CCDSiCCDS19558.1.
RefSeqiNP_001028374.1. NM_001033202.3.
UniGeneiMm.240421.

Genome annotation databases

EnsembliENSMUST00000031588; ENSMUSP00000031588; ENSMUSG00000029592.
GeneIDi100756.
KEGGimmu:100756.
UCSCiuc008yzc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK078164 mRNA. Translation: BAC37154.1.
AK144578 mRNA. Translation: BAE25944.1.
AK162153 mRNA. Translation: BAE36756.1.
AK162269 mRNA. Translation: BAE36827.1.
BC038606 mRNA. Translation: AAH38606.1. Different initiation.
CCDSiCCDS19558.1.
RefSeqiNP_001028374.1. NM_001033202.3.
UniGeneiMm.240421.

3D structure databases

ProteinModelPortaliQ3UN04.
SMRiQ3UN04. Positions 69-331, 415-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000031588.

Protein family/group databases

MEROPSiC19.060.

Proteomic databases

MaxQBiQ3UN04.
PaxDbiQ3UN04.
PRIDEiQ3UN04.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031588; ENSMUSP00000031588; ENSMUSG00000029592.
GeneIDi100756.
KEGGimmu:100756.
UCSCiuc008yzc.1. mouse.

Organism-specific databases

CTDi84749.
MGIiMGI:2140991. Usp30.

Phylogenomic databases

eggNOGiNOG259866.
GeneTreeiENSGT00550000075075.
HOGENOMiHOG000065744.
InParanoidiQ3UN04.
KOiK11851.
OMAiMKNWGVL.
OrthoDBiEOG7P8P7N.
PhylomeDBiQ3UN04.
TreeFamiTF105781.

Miscellaneous databases

ChiTaRSiUsp30. mouse.
NextBioi354628.
PROiQ3UN04.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UN04.
GenevisibleiQ3UN04. MM.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Lung and Medulla oblongata.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Salivary gland.
  3. "A small natural molecule promotes mitochondrial fusion through inhibition of the deubiquitinase USP30."
    Yue W., Chen Z., Liu H., Yan C., Chen M., Feng D., Yan C., Wu H., Du L., Wang Y., Liu J., Huang X., Xia L., Liu L., Wang X., Jin H., Wang J., Song Z., Hao X., Chen Q.
    Cell Res. 24:482-496(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, ACTIVE SITE, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-77; CYS-234; CYS-284 AND HIS-452.

Entry informationi

Entry nameiUBP30_MOUSE
AccessioniPrimary (citable) accession number: Q3UN04
Secondary accession number(s): Q3TS48
, Q3TSB9, Q8BVI3, Q8CHW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: October 11, 2005
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.