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Protein

Lysocardiolipin acyltransferase 1

Gene

Lclat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages.2 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), Glycerol-3-phosphate acyltransferase 4 (Gpat4), Glycerol-3-phosphate acyltransferase 3 (Gpat3), Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (Agpat3), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (Agpat2), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (Agpat1), Lysocardiolipin acyltransferase 1 (Lclat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (Agpat5), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (Agpat4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 2 (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 1 (Cds1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Developmental protein, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1482798 Acyl chain remodeling of CL
R-MMU-1483166 Synthesis of PA
UniPathwayiUPA00557; UER00613

Chemistry databases

SwissLipidsiSLP:000000291

Names & Taxonomyi

Protein namesi
Recommended name:
Lysocardiolipin acyltransferase 1 (EC:2.3.1.-, EC:2.3.1.51)
Alternative name(s):
Acyl-CoA:lysocardiolipin acyltransferase 1
Gene namesi
Name:Lclat1
Synonyms:Alcat1, Gm91, Lycat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2684937 Lclat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei9 – 29HelicalSequence analysisAdd BLAST21
Transmembranei48 – 68HelicalSequence analysisAdd BLAST21
Transmembranei309 – 329HelicalSequence analysisAdd BLAST21
Transmembranei336 – 356HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002915781 – 376Lysocardiolipin acyltransferase 1Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei183N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

EPDiQ3UN02
MaxQBiQ3UN02
PaxDbiQ3UN02
PRIDEiQ3UN02

PTM databases

iPTMnetiQ3UN02
PhosphoSitePlusiQ3UN02

Expressioni

Tissue specificityi

Widely expressed with highest expression in heart, liver and E12.5 aorta-gonad-mesonephros and lower levels in the E16 fetal liver and adult bone marrow. In bone marrow, highest levels are found in B-cells compared with whole bone marrow, T-cells, erythrocytes, and granulocytes.3 Publications

Gene expression databases

BgeeiENSMUSG00000054469
CleanExiMM_LYCAT
ExpressionAtlasiQ3UN02 baseline and differential
GenevisibleiQ3UN02 MM

Interactioni

Protein-protein interaction databases

BioGridi2303492 interactors.
STRINGi10090.ENSMUSP00000068690

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi85 – 90HXXXXD motif6

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00890000139404
HOGENOMiHOG000290725
HOVERGENiHBG055624
InParanoidiQ3UN02
KOiK13513
OMAiFGWAMQV
OrthoDBiEOG091G0C4Z
PhylomeDBiQ3UN02
TreeFamiTF314346

Family and domain databases

InterProiView protein in InterPro
IPR032098 Acyltransf_C
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF16076 Acyltransf_C, 1 hit
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit

Sequencei

Sequence statusi: Complete.

Q3UN02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSWKGIYFI LFLFAGSFFG SIFMLGPILP LMFINLSWYR WISSRLVATW
60 70 80 90 100
LTLPVALLET MFGVRVVITG DAFVPGERSV IIMNHRTRVD WMFLWNCLMR
110 120 130 140 150
YSYLRVEKIC LKSSLKSVPG FGWAMQVAAF IFIHRKWKDD KSHFEDMIDY
160 170 180 190 200
FCAIHEPLQL LIFPEGTDLT ENNKARSNDF AEKNGLQKYE YVLHPRTTGF
210 220 230 240 250
TFVVDRLREG KNLDAVHDIT VAYPYNIPQT EKHLLLGDFP KEIHFHVQRY
260 270 280 290 300
PADSLPTSKE DLQLWCHRRW EEKEERLRSF YQGEKNFHFT GQSTVPPCKS
310 320 330 340 350
ELRVLVVKLL SIVYWALFCS AMCLLIYLYS PVRWYFIISI VFFVLQERIF
360 370
GGLEIIELAC YRFLHKHPHL NSKKNE
Length:376
Mass (Da):44,400
Last modified:June 26, 2007 - v2
Checksum:iC2E7AAC40145CD7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC112949 Genomic DNA No translation available.
AK144580 mRNA Translation: BAE25946.1
CCDSiCCDS37689.1
RefSeqiNP_001074540.1, NM_001081071.2
NP_001171438.1, NM_001177967.1
NP_001171439.1, NM_001177968.1
XP_011244712.1, XM_011246410.2
UniGeneiMm.329194

Genome annotation databases

EnsembliENSMUST00000067545; ENSMUSP00000068690; ENSMUSG00000054469
GeneIDi225010
KEGGimmu:225010
UCSCiuc008dnh.2 mouse

Similar proteinsi

Entry informationi

Entry nameiLCLT1_MOUSE
AccessioniPrimary (citable) accession number: Q3UN02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: February 28, 2018
This is version 98 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome