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Protein

Lysocardiolipin acyltransferase 1

Gene

Lclat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages.2 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.1 Publication

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (Gpam), Glycerol-3-phosphate acyltransferase 4 (Gpat4), Glycerol-3-phosphate acyltransferase 3 (Gpat3), Glycerol-3-phosphate acyltransferase 2, mitochondrial (Gpat2)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (Agpat3), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (Agpat2), 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (Agpat1), Lysocardiolipin acyltransferase 1 (Lclat1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (Agpat5), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (Agpat4)
  3. Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase, mitochondrial (Tamm41), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 2 (Cds2), Phosphatidate cytidylyltransferase (Cds2), Phosphatidate cytidylyltransferase 1 (Cds1)
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Developmental protein, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

ReactomeiR-MMU-1482798. Acyl chain remodeling of CL.
R-MMU-1483166. Synthesis of PA.
R-MMU-75109. Triglyceride Biosynthesis.
UniPathwayiUPA00557; UER00613.

Chemistry

SwissLipidsiSLP:000000291.

Names & Taxonomyi

Protein namesi
Recommended name:
Lysocardiolipin acyltransferase 1 (EC:2.3.1.-, EC:2.3.1.51)
Alternative name(s):
Acyl-CoA:lysocardiolipin acyltransferase 1
Gene namesi
Name:Lclat1
Synonyms:Alcat1, Gm91, Lycat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2684937. Lclat1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Transmembranei48 – 6821HelicalSequence analysisAdd
BLAST
Transmembranei309 – 32921HelicalSequence analysisAdd
BLAST
Transmembranei336 – 35621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Lysocardiolipin acyltransferase 1PRO_0000291578Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence analysis
Modified residuei183 – 1831N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein

Proteomic databases

EPDiQ3UN02.
MaxQBiQ3UN02.
PaxDbiQ3UN02.
PRIDEiQ3UN02.

PTM databases

iPTMnetiQ3UN02.
PhosphoSiteiQ3UN02.

Expressioni

Tissue specificityi

Widely expressed with highest expression in heart, liver and E12.5 aorta-gonad-mesonephros and lower levels in the E16 fetal liver and adult bone marrow. In bone marrow, highest levels are found in B-cells compared with whole bone marrow, T-cells, erythrocytes, and granulocytes.3 Publications

Gene expression databases

BgeeiQ3UN02.
CleanExiMM_LYCAT.
ExpressionAtlasiQ3UN02. baseline and differential.
GenevisibleiQ3UN02. MM.

Interactioni

Protein-protein interaction databases

BioGridi230349. 2 interactions.
STRINGi10090.ENSMUSP00000068690.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi85 – 906HXXXXD motif

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
GeneTreeiENSGT00530000062943.
HOGENOMiHOG000290725.
HOVERGENiHBG055624.
InParanoidiQ3UN02.
KOiK13513.
OMAiIPQSEKH.
PhylomeDBiQ3UN02.
TreeFamiTF314346.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3UN02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSWKGIYFI LFLFAGSFFG SIFMLGPILP LMFINLSWYR WISSRLVATW
60 70 80 90 100
LTLPVALLET MFGVRVVITG DAFVPGERSV IIMNHRTRVD WMFLWNCLMR
110 120 130 140 150
YSYLRVEKIC LKSSLKSVPG FGWAMQVAAF IFIHRKWKDD KSHFEDMIDY
160 170 180 190 200
FCAIHEPLQL LIFPEGTDLT ENNKARSNDF AEKNGLQKYE YVLHPRTTGF
210 220 230 240 250
TFVVDRLREG KNLDAVHDIT VAYPYNIPQT EKHLLLGDFP KEIHFHVQRY
260 270 280 290 300
PADSLPTSKE DLQLWCHRRW EEKEERLRSF YQGEKNFHFT GQSTVPPCKS
310 320 330 340 350
ELRVLVVKLL SIVYWALFCS AMCLLIYLYS PVRWYFIISI VFFVLQERIF
360 370
GGLEIIELAC YRFLHKHPHL NSKKNE
Length:376
Mass (Da):44,400
Last modified:June 26, 2007 - v2
Checksum:iC2E7AAC40145CD7E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC112949 Genomic DNA. No translation available.
AK144580 mRNA. Translation: BAE25946.1.
CCDSiCCDS37689.1.
RefSeqiNP_001074540.1. NM_001081071.2.
NP_001171438.1. NM_001177967.1.
NP_001171439.1. NM_001177968.1.
XP_011244712.1. XM_011246410.1.
UniGeneiMm.329194.

Genome annotation databases

EnsembliENSMUST00000067545; ENSMUSP00000068690; ENSMUSG00000054469.
GeneIDi225010.
KEGGimmu:225010.
UCSCiuc008dnh.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC112949 Genomic DNA. No translation available.
AK144580 mRNA. Translation: BAE25946.1.
CCDSiCCDS37689.1.
RefSeqiNP_001074540.1. NM_001081071.2.
NP_001171438.1. NM_001177967.1.
NP_001171439.1. NM_001177968.1.
XP_011244712.1. XM_011246410.1.
UniGeneiMm.329194.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230349. 2 interactions.
STRINGi10090.ENSMUSP00000068690.

Chemistry

SwissLipidsiSLP:000000291.

PTM databases

iPTMnetiQ3UN02.
PhosphoSiteiQ3UN02.

Proteomic databases

EPDiQ3UN02.
MaxQBiQ3UN02.
PaxDbiQ3UN02.
PRIDEiQ3UN02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000067545; ENSMUSP00000068690; ENSMUSG00000054469.
GeneIDi225010.
KEGGimmu:225010.
UCSCiuc008dnh.2. mouse.

Organism-specific databases

CTDi253558.
MGIiMGI:2684937. Lclat1.

Phylogenomic databases

eggNOGiKOG1505. Eukaryota.
COG0204. LUCA.
GeneTreeiENSGT00530000062943.
HOGENOMiHOG000290725.
HOVERGENiHBG055624.
InParanoidiQ3UN02.
KOiK13513.
OMAiIPQSEKH.
PhylomeDBiQ3UN02.
TreeFamiTF314346.

Enzyme and pathway databases

UniPathwayiUPA00557; UER00613.
ReactomeiR-MMU-1482798. Acyl chain remodeling of CL.
R-MMU-1483166. Synthesis of PA.
R-MMU-75109. Triglyceride Biosynthesis.

Miscellaneous databases

ChiTaRSiLclat1. mouse.
PROiQ3UN02.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UN02.
CleanExiMM_LYCAT.
ExpressionAtlasiQ3UN02. baseline and differential.
GenevisibleiQ3UN02. MM.

Family and domain databases

InterProiIPR032098. Acyltransf_C.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF16076. Acyltransf_C. 1 hit.
PF01553. Acyltransferase. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-281.
    Tissue: Lung.
  3. "A novel cardiolipin-remodeling pathway revealed by a gene encoding an endoplasmic reticulum-associated acyl-CoA:lysocardiolipin acyltransferase (ALCAT1) in mouse."
    Cao J., Liu Y., Lockwood J., Burn P., Shi Y.
    J. Biol. Chem. 279:31727-31734(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  4. "Functional characterization of human 1-acylglycerol-3-phosphate acyltransferase isoform 8: cloning, tissue distribution, gene structure, and enzymatic activity."
    Agarwal A.K., Barnes R.I., Garg A.
    Arch. Biochem. Biophys. 449:64-76(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Mouse lysocardiolipin acyltransferase controls the development of hematopoietic and endothelial lineages during in vitro embryonic stem-cell differentiation."
    Wang C., Faloon P.W., Tan Z., Lv Y., Zhang P., Ge Y., Deng H., Xiong J.-W.
    Blood 110:3601-3609(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Heart, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiLCLT1_MOUSE
AccessioniPrimary (citable) accession number: Q3UN02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: July 6, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.