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Protein

Echinoderm microtubule-associated protein-like 4

Gene

Eml4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

GO - Molecular functioni

GO - Biological processi

  • negative regulation of microtubule depolymerization Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 4
Short name:
EMAP-4
Gene namesi
Name:Eml4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1926048. Eml4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002843921 – 988Echinoderm microtubule-associated protein-like 4Add BLAST988

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei7PhosphoserineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei61PhosphoserineBy similarity1
Modified residuei79PhosphoserineCombined sources1
Modified residuei96PhosphothreonineBy similarity1
Modified residuei144PhosphoserineCombined sources1
Modified residuei146PhosphoserineCombined sources1
Modified residuei171PhosphoserineBy similarity1
Modified residuei200PhosphoserineBy similarity1
Modified residuei201PhosphothreonineBy similarity1
Modified residuei237PhosphotyrosineBy similarity1
Modified residuei248PhosphothreonineBy similarity1
Modified residuei906PhosphoserineBy similarity1
Modified residuei908PhosphoserineCombined sources1
Modified residuei914PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3UMY5.
MaxQBiQ3UMY5.
PaxDbiQ3UMY5.
PeptideAtlasiQ3UMY5.
PRIDEiQ3UMY5.

PTM databases

iPTMnetiQ3UMY5.
PhosphoSitePlusiQ3UMY5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032624.
CleanExiMM_EML4.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219641. 1 interactor.
STRINGi10090.ENSMUSP00000094528.

Structurei

3D structure databases

ProteinModelPortaliQ3UMY5.
SMRiQ3UMY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati318 – 367WD 1Add BLAST50
Repeati372 – 415WD 2Add BLAST44
Repeati418 – 459WD 3Add BLAST42
Repeati465 – 503WD 4Add BLAST39
Repeati518 – 557WD 5Add BLAST40
Repeati601 – 640WD 6Add BLAST40
Repeati684 – 723WD 7Add BLAST40
Repeati730 – 769WD 8Add BLAST40
Repeati843 – 883WD 9Add BLAST41

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410KDGE. Eukaryota.
COG2319. LUCA.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ3UMY5.
KOiK15420.
PhylomeDBiQ3UMY5.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UMY5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR
60 70 80 90 100
RLAISEDHVA SVKKSMPSKG QPSLREAISM SCITNGSGIS RKQNHTSSVS
110 120 130 140 150
IARKETLSSA AKSGTEKKKE KPQGQREKKE DSHSNDQSPQ IRASPSPQPS
160 170 180 190 200
SQPLQINRQT PESKSSAPIK SIKRPPTAEK SHNSWENSDD SRNKLMKTVS
210 220 230 240 250
TSKLISKVIK NADKHKDVIV NQAKMSTREK NSQEGEYIKM FMRGRPITMF
260 270 280 290 300
IPSDVDNYDD IRTELPPEKL KLEWVYGYRG KDCRANVYLL PTGEIVYFIA
310 320 330 340 350
SVVVLFNYEE RTQRHYLGHT DCVRCLAVHP DKIRIATGQI AGVDKDGRPL
360 370 380 390 400
QPHVRVWDSV SLTTLHVIGL GTFERGVGCL DFSKADSGVH LCVIDDSNEH
410 420 430 440 450
MLTVWDWQKK SKIAEIKTTN EVVLAVEFHP TDANTIITCG KSHIFFWTWS
460 470 480 490 500
GNSLTRKQGI FGKYEKPKFV QCLAFLGNGD VLTGDSGGVM LIWSKTMVEP
510 520 530 540 550
PPGKGPKGVY QINRQIKAHD GSVFTLCQMR NGMLLTGGGK DRKIILWDHD
560 570 580 590 600
LNLEREIEVP DQYGTIRAVA EGRAEQFLVG TSRNFILRGT FNDGFQIEVQ
610 620 630 640 650
GHRDELWGLA THPFKDLLLT CAQDRQVCMW NSVEHRLEWT RLVDEPGHCA
660 670 680 690 700
DFHPSGTVVA IGTHSGRWFV LDAETRDLVS IHTDGNEQLS VMRYSVDGTL
710 720 730 740 750
LAVGSHDNFI YLYTVLENGR KYSRYGKCTG HSSYITHLDW SPDNKHIMSN
760 770 780 790 800
SGDYEILYWD IENGCKLIRN RSDCKDIDWT TYTCVLGFQV FGVWPEGSDG
810 820 830 840 850
TDINALVRSH NRRVIAVADD FCKVHLFQYP CSKAKAPSHK YSAHSSHVTN
860 870 880 890 900
VSFTHNDSHL ISTGGKDMSI IQWKLVEKLP VPQNEVITDA SVTKTPASSS
910 920 930 940 950
ETARPSNSPP LPPSLPLTGT AEEESRMGSS PTLVENSLEQ IAEPSEEQSE
960 970 980
WGSEDLGVVI DEEPASELSE TQGATELPEE ERGITPLC
Length:988
Mass (Da):110,027
Last modified:October 11, 2005 - v1
Checksum:i950336CFC1C178D5
GO
Isoform 2 (identifier: Q3UMY5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-171: Missing.
     223-233: Missing.

Note: No experimental confirmation available.
Show »
Length:919
Mass (Da):102,359
Checksum:iE60BB52B9525D4E4
GO
Isoform 3 (identifier: Q3UMY5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MDGFAGSL → MNRVSSDPVAIP
     114-171: Missing.

Note: No experimental confirmation available.
Show »
Length:934
Mass (Da):104,109
Checksum:i62D2D4042B771FB2
GO
Isoform 4 (identifier: Q3UMY5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-214: SGTEKKKEKP...ISKVIKNADK → R
     223-233: Missing.

Note: No experimental confirmation available.
Show »
Length:876
Mass (Da):97,563
Checksum:i4576D56445E59DAB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti603R → T in AAH67011 (PubMed:15489334).Curated1
Sequence conflicti603R → T in AAH66099 (PubMed:15489334).Curated1
Sequence conflicti603R → T in AAH79619 (PubMed:15489334).Curated1
Sequence conflicti603R → T in AAH65178 (PubMed:15489334).Curated1
Sequence conflicti603R → T in AAH70427 (PubMed:15489334).Curated1
Sequence conflicti804N → I in AAH67011 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0244841 – 8MDGFAGSL → MNRVSSDPVAIP in isoform 3. 1 Publication8
Alternative sequenceiVSP_024485113 – 214SGTEK…KNADK → R in isoform 4. 1 PublicationAdd BLAST102
Alternative sequenceiVSP_024486114 – 171Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_024487223 – 233Missing in isoform 2 and isoform 4. 1 PublicationAdd BLAST11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144604 mRNA. Translation: BAE25963.1.
BC065178 mRNA. Translation: AAH65178.1.
BC066099 mRNA. Translation: AAH66099.1.
BC067011 mRNA. Translation: AAH67011.1.
BC070427 mRNA. Translation: AAH70427.1.
BC079619 mRNA. Translation: AAH79619.1.
CCDSiCCDS37708.1. [Q3UMY5-4]
CCDS50192.1. [Q3UMY5-1]
CCDS50193.1. [Q3UMY5-2]
RefSeqiNP_001107834.1. NM_001114362.1.
NP_001273496.1. NM_001286567.1.
NP_955760.3. NM_199466.3.
UniGeneiMm.295565.

Genome annotation databases

GeneIDi78798.
KEGGimmu:78798.
UCSCiuc008drx.2. mouse. [Q3UMY5-4]
uc008dry.2. mouse. [Q3UMY5-3]
uc008dsb.2. mouse. [Q3UMY5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144604 mRNA. Translation: BAE25963.1.
BC065178 mRNA. Translation: AAH65178.1.
BC066099 mRNA. Translation: AAH66099.1.
BC067011 mRNA. Translation: AAH67011.1.
BC070427 mRNA. Translation: AAH70427.1.
BC079619 mRNA. Translation: AAH79619.1.
CCDSiCCDS37708.1. [Q3UMY5-4]
CCDS50192.1. [Q3UMY5-1]
CCDS50193.1. [Q3UMY5-2]
RefSeqiNP_001107834.1. NM_001114362.1.
NP_001273496.1. NM_001286567.1.
NP_955760.3. NM_199466.3.
UniGeneiMm.295565.

3D structure databases

ProteinModelPortaliQ3UMY5.
SMRiQ3UMY5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219641. 1 interactor.
STRINGi10090.ENSMUSP00000094528.

PTM databases

iPTMnetiQ3UMY5.
PhosphoSitePlusiQ3UMY5.

Proteomic databases

EPDiQ3UMY5.
MaxQBiQ3UMY5.
PaxDbiQ3UMY5.
PeptideAtlasiQ3UMY5.
PRIDEiQ3UMY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi78798.
KEGGimmu:78798.
UCSCiuc008drx.2. mouse. [Q3UMY5-4]
uc008dry.2. mouse. [Q3UMY5-3]
uc008dsb.2. mouse. [Q3UMY5-2]

Organism-specific databases

CTDi27436.
MGIiMGI:1926048. Eml4.

Phylogenomic databases

eggNOGiENOG410KDGE. Eukaryota.
COG2319. LUCA.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ3UMY5.
KOiK15420.
PhylomeDBiQ3UMY5.

Miscellaneous databases

ChiTaRSiEml4. mouse.
PROiQ3UMY5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032624.
CleanExiMM_EML4.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEMAL4_MOUSE
AccessioniPrimary (citable) accession number: Q3UMY5
Secondary accession number(s): Q6NS73
, Q6NXL8, Q6NZJ8, Q6P1A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.