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Protein

Echinoderm microtubule-associated protein-like 4

Gene

Eml4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May modify the assembly dynamics of microtubules, such that microtubules are slightly longer, but more dynamic.By similarity

GO - Biological processi

  • negative regulation of microtubule depolymerization Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Echinoderm microtubule-associated protein-like 4
Short name:
EMAP-4
Gene namesi
Name:Eml4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1926048. Eml4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 988988Echinoderm microtubule-associated protein-like 4PRO_0000284392Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei7 – 71PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei16 – 161PhosphoserineBy similarity
Modified residuei61 – 611PhosphoserineBy similarity
Modified residuei79 – 791PhosphoserineCombined sources
Modified residuei96 – 961PhosphothreonineBy similarity
Modified residuei144 – 1441PhosphoserineCombined sources
Modified residuei146 – 1461PhosphoserineCombined sources
Modified residuei171 – 1711PhosphoserineBy similarity
Modified residuei200 – 2001PhosphoserineBy similarity
Modified residuei201 – 2011PhosphothreonineBy similarity
Modified residuei237 – 2371PhosphotyrosineBy similarity
Modified residuei248 – 2481PhosphothreonineBy similarity
Modified residuei906 – 9061PhosphoserineBy similarity
Modified residuei908 – 9081PhosphoserineCombined sources
Modified residuei914 – 9141PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ3UMY5.
MaxQBiQ3UMY5.
PaxDbiQ3UMY5.
PeptideAtlasiQ3UMY5.
PRIDEiQ3UMY5.

PTM databases

iPTMnetiQ3UMY5.
PhosphoSiteiQ3UMY5.

Expressioni

Gene expression databases

BgeeiQ3UMY5.
CleanExiMM_EML4.

Interactioni

Protein-protein interaction databases

BioGridi219641. 1 interaction.
STRINGi10090.ENSMUSP00000094528.

Structurei

3D structure databases

ProteinModelPortaliQ3UMY5.
SMRiQ3UMY5. Positions 16-44, 232-876.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati318 – 36750WD 1Add
BLAST
Repeati372 – 41544WD 2Add
BLAST
Repeati418 – 45942WD 3Add
BLAST
Repeati465 – 50339WD 4Add
BLAST
Repeati518 – 55740WD 5Add
BLAST
Repeati601 – 64040WD 6Add
BLAST
Repeati684 – 72340WD 7Add
BLAST
Repeati730 – 76940WD 8Add
BLAST
Repeati843 – 88341WD 9Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat EMAP family.Curated
Contains 9 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiENOG410KDGE. Eukaryota.
COG2319. LUCA.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ3UMY5.
KOiK15420.
PhylomeDBiQ3UMY5.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q3UMY5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDGFAGSLDD SISAASTSDV QDRLSALESR VQQQEDEITV LKAALADVLR
60 70 80 90 100
RLAISEDHVA SVKKSMPSKG QPSLREAISM SCITNGSGIS RKQNHTSSVS
110 120 130 140 150
IARKETLSSA AKSGTEKKKE KPQGQREKKE DSHSNDQSPQ IRASPSPQPS
160 170 180 190 200
SQPLQINRQT PESKSSAPIK SIKRPPTAEK SHNSWENSDD SRNKLMKTVS
210 220 230 240 250
TSKLISKVIK NADKHKDVIV NQAKMSTREK NSQEGEYIKM FMRGRPITMF
260 270 280 290 300
IPSDVDNYDD IRTELPPEKL KLEWVYGYRG KDCRANVYLL PTGEIVYFIA
310 320 330 340 350
SVVVLFNYEE RTQRHYLGHT DCVRCLAVHP DKIRIATGQI AGVDKDGRPL
360 370 380 390 400
QPHVRVWDSV SLTTLHVIGL GTFERGVGCL DFSKADSGVH LCVIDDSNEH
410 420 430 440 450
MLTVWDWQKK SKIAEIKTTN EVVLAVEFHP TDANTIITCG KSHIFFWTWS
460 470 480 490 500
GNSLTRKQGI FGKYEKPKFV QCLAFLGNGD VLTGDSGGVM LIWSKTMVEP
510 520 530 540 550
PPGKGPKGVY QINRQIKAHD GSVFTLCQMR NGMLLTGGGK DRKIILWDHD
560 570 580 590 600
LNLEREIEVP DQYGTIRAVA EGRAEQFLVG TSRNFILRGT FNDGFQIEVQ
610 620 630 640 650
GHRDELWGLA THPFKDLLLT CAQDRQVCMW NSVEHRLEWT RLVDEPGHCA
660 670 680 690 700
DFHPSGTVVA IGTHSGRWFV LDAETRDLVS IHTDGNEQLS VMRYSVDGTL
710 720 730 740 750
LAVGSHDNFI YLYTVLENGR KYSRYGKCTG HSSYITHLDW SPDNKHIMSN
760 770 780 790 800
SGDYEILYWD IENGCKLIRN RSDCKDIDWT TYTCVLGFQV FGVWPEGSDG
810 820 830 840 850
TDINALVRSH NRRVIAVADD FCKVHLFQYP CSKAKAPSHK YSAHSSHVTN
860 870 880 890 900
VSFTHNDSHL ISTGGKDMSI IQWKLVEKLP VPQNEVITDA SVTKTPASSS
910 920 930 940 950
ETARPSNSPP LPPSLPLTGT AEEESRMGSS PTLVENSLEQ IAEPSEEQSE
960 970 980
WGSEDLGVVI DEEPASELSE TQGATELPEE ERGITPLC
Length:988
Mass (Da):110,027
Last modified:October 11, 2005 - v1
Checksum:i950336CFC1C178D5
GO
Isoform 2 (identifier: Q3UMY5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     114-171: Missing.
     223-233: Missing.

Note: No experimental confirmation available.
Show »
Length:919
Mass (Da):102,359
Checksum:iE60BB52B9525D4E4
GO
Isoform 3 (identifier: Q3UMY5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-8: MDGFAGSL → MNRVSSDPVAIP
     114-171: Missing.

Note: No experimental confirmation available.
Show »
Length:934
Mass (Da):104,109
Checksum:i62D2D4042B771FB2
GO
Isoform 4 (identifier: Q3UMY5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     113-214: SGTEKKKEKP...ISKVIKNADK → R
     223-233: Missing.

Note: No experimental confirmation available.
Show »
Length:876
Mass (Da):97,563
Checksum:i4576D56445E59DAB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti603 – 6031R → T in AAH67011 (PubMed:15489334).Curated
Sequence conflicti603 – 6031R → T in AAH66099 (PubMed:15489334).Curated
Sequence conflicti603 – 6031R → T in AAH79619 (PubMed:15489334).Curated
Sequence conflicti603 – 6031R → T in AAH65178 (PubMed:15489334).Curated
Sequence conflicti603 – 6031R → T in AAH70427 (PubMed:15489334).Curated
Sequence conflicti804 – 8041N → I in AAH67011 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 88MDGFAGSL → MNRVSSDPVAIP in isoform 3. 1 PublicationVSP_024484
Alternative sequencei113 – 214102SGTEK…KNADK → R in isoform 4. 1 PublicationVSP_024485Add
BLAST
Alternative sequencei114 – 17158Missing in isoform 2 and isoform 3. 1 PublicationVSP_024486Add
BLAST
Alternative sequencei223 – 23311Missing in isoform 2 and isoform 4. 1 PublicationVSP_024487Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144604 mRNA. Translation: BAE25963.1.
BC065178 mRNA. Translation: AAH65178.1.
BC066099 mRNA. Translation: AAH66099.1.
BC067011 mRNA. Translation: AAH67011.1.
BC070427 mRNA. Translation: AAH70427.1.
BC079619 mRNA. Translation: AAH79619.1.
CCDSiCCDS37708.1. [Q3UMY5-4]
CCDS50192.1. [Q3UMY5-1]
CCDS50193.1. [Q3UMY5-2]
RefSeqiNP_001107834.1. NM_001114362.1.
NP_001273496.1. NM_001286567.1.
NP_955760.3. NM_199466.3.
UniGeneiMm.295565.

Genome annotation databases

GeneIDi78798.
KEGGimmu:78798.
UCSCiuc008drx.2. mouse. [Q3UMY5-4]
uc008dry.2. mouse. [Q3UMY5-3]
uc008dsb.2. mouse. [Q3UMY5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144604 mRNA. Translation: BAE25963.1.
BC065178 mRNA. Translation: AAH65178.1.
BC066099 mRNA. Translation: AAH66099.1.
BC067011 mRNA. Translation: AAH67011.1.
BC070427 mRNA. Translation: AAH70427.1.
BC079619 mRNA. Translation: AAH79619.1.
CCDSiCCDS37708.1. [Q3UMY5-4]
CCDS50192.1. [Q3UMY5-1]
CCDS50193.1. [Q3UMY5-2]
RefSeqiNP_001107834.1. NM_001114362.1.
NP_001273496.1. NM_001286567.1.
NP_955760.3. NM_199466.3.
UniGeneiMm.295565.

3D structure databases

ProteinModelPortaliQ3UMY5.
SMRiQ3UMY5. Positions 16-44, 232-876.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219641. 1 interaction.
STRINGi10090.ENSMUSP00000094528.

PTM databases

iPTMnetiQ3UMY5.
PhosphoSiteiQ3UMY5.

Proteomic databases

EPDiQ3UMY5.
MaxQBiQ3UMY5.
PaxDbiQ3UMY5.
PeptideAtlasiQ3UMY5.
PRIDEiQ3UMY5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi78798.
KEGGimmu:78798.
UCSCiuc008drx.2. mouse. [Q3UMY5-4]
uc008dry.2. mouse. [Q3UMY5-3]
uc008dsb.2. mouse. [Q3UMY5-2]

Organism-specific databases

CTDi27436.
MGIiMGI:1926048. Eml4.

Phylogenomic databases

eggNOGiENOG410KDGE. Eukaryota.
COG2319. LUCA.
HOGENOMiHOG000294061.
HOVERGENiHBG051470.
InParanoidiQ3UMY5.
KOiK15420.
PhylomeDBiQ3UMY5.

Miscellaneous databases

ChiTaRSiEml4. mouse.
PROiQ3UMY5.
SOURCEiSearch...

Gene expression databases

BgeeiQ3UMY5.
CleanExiMM_EML4.

Family and domain databases

Gene3Di2.130.10.10. 3 hits.
InterProiIPR005108. HELP.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF03451. HELP. 1 hit.
PF00400. WD40. 5 hits.
[Graphical view]
SMARTiSM00320. WD40. 9 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF50998. SSF50998. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 4 hits.
PS50294. WD_REPEATS_REGION. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-79; SER-144; SER-146 AND SER-908, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiEMAL4_MOUSE
AccessioniPrimary (citable) accession number: Q3UMY5
Secondary accession number(s): Q6NS73
, Q6NXL8, Q6NZJ8, Q6P1A7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: October 11, 2005
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.