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Protein

Ceroid-lipofuscinosis neuronal protein 5 homolog

Gene

Cln5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.By similarity

GO - Molecular functioni

GO - Biological processi

  • brain development Source: UniProtKB
  • glycosylation Source: UniProtKB
  • lysosomal lumen acidification Source: UniProtKB
  • lysosome organization Source: MGI
  • neurogenesis Source: UniProtKB
  • positive regulation of GTP binding Source: MGI
  • retrograde transport, endosome to Golgi Source: MGI
  • signal peptide processing Source: UniProtKB
  • visual perception Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Ceroid-lipofuscinosis neuronal protein 5 homolog
Short name:
Protein CLN5
Cleaved into the following chain:
Gene namesi
Name:Cln5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:2442253. Cln5.

Subcellular locationi

Ceroid-lipofuscinosis neuronal protein 5 homolog, secreted form :
Ceroid-lipofuscinosis neuronal protein 5 homolog :
  • Membrane By similarity; Single-pass type II membrane protein By similarity

  • Note: An amphipathic anchor region facilitates its association with the membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicBy similarityAdd BLAST13
Transmembranei14 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini31 – 341LumenalBy similarityAdd BLAST311

GO - Cellular componenti

  • cytosol Source: GOC
  • endoplasmic reticulum Source: UniProtKB
  • extracellular exosome Source: MGI
  • Golgi apparatus Source: UniProtKB
  • integral component of membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • lysosome Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
  • vacuolar lumen Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

Pathology & Biotechi

Keywords - Diseasei

Neurodegeneration, Neuronal ceroid lipofuscinosis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000438010? – 341Ceroid-lipofuscinosis neuronal protein 5 homolog, secreted form
ChainiPRO_00003304711 – 341Ceroid-lipofuscinosis neuronal protein 5 homologAdd BLAST341

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Glycosylationi126N-linked (GlcNAc...)Sequence analysis1
Glycosylationi161N-linked (GlcNAc...)Sequence analysis1
Glycosylationi186N-linked (GlcNAc...)Sequence analysis1
Glycosylationi238N-linked (GlcNAc...)Sequence analysis1
Glycosylationi254N-linked (GlcNAc...)Sequence analysis1
Glycosylationi264N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated with both high mannose and complex type sugars. Glycosylation is important for proper folding and trafficking to the lysosomes.By similarity
Ceroid-lipofuscinosis neuronal protein 5 homolog: The type II membrane signal anchor is proteolytically cleaved to produce a mature form that is transported to the lysosomes (Ceroid-lipofuscinosis neuronal protein 5 homolog, secreted form).By similarity
Can undergo proteolytic cleavage at the C-terminus, probably by a cysteine protease and may involve the removal of approximately 10-15 residues from the C-terminal end (PubMed:26342652).1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ3UMW8.
MaxQBiQ3UMW8.
PaxDbiQ3UMW8.
PRIDEiQ3UMW8.

PTM databases

PhosphoSitePlusiQ3UMW8.

Expressioni

Tissue specificityi

Heart, kidney, liver, spleen, muscle and rectum (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000022125.
ExpressionAtlasiQ3UMW8. baseline and differential.
GenevisibleiQ3UMW8. MM.

Interactioni

Subunit structurei

Interacts with PPT1, TPP1, CLN3, CLN6, CLN8, ATP5A1 and ATP5B (PubMed:19941651). Interacts with SORT1, RAB5A and RAB7A (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022721.

Structurei

3D structure databases

ProteinModelPortaliQ3UMW8.
SMRiQ3UMW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni287 – 326Membrane-anchoringBy similarityAdd BLAST40

Sequence similaritiesi

Belongs to the CLN5 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJEH. Eukaryota.
ENOG4110RH0. LUCA.
GeneTreeiENSGT00390000010065.
HOGENOMiHOG000060233.
HOVERGENiHBG005345.
InParanoidiQ3UMW8.
KOiK12390.
OMAiWVKQDNE.
OrthoDBiEOG091G0B86.
PhylomeDBiQ3UMW8.
TreeFamiTF330864.

Family and domain databases

InterProiIPR026138. CLN5.
[Graphical view]
PANTHERiPTHR15380. PTHR15380. 1 hit.
PfamiPF15014. CLN5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q3UMW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRGGPCGAH WRPALALALL GLATILGASP TSGQRWPVPY KRFSFRPKTD
60 70 80 90 100
PYCQAKYTFC PTGSPIPVMK DNDVIEVLRL QAPIWEFKYG DLLGHFKLMH
110 120 130 140 150
DAVGFRSTLT GKNYTIEWYE LFQLGNCTFP HLRPDKSAPF WCNQGAACFF
160 170 180 190 200
EGIDDKHWKE NGTLSVVATI SGNTFNKVAE WVKQDNETGI YYETWTVRAG
210 220 230 240 250
PGQGAQTWFE SYDCSNFVLR TYKKLAEFGT EFKKIETNYT KIFLYSGEPI
260 270 280 290 300
YLGNETSIFG PKGNKTLALA IKKFYGPFRP YLSTKDFLMN FLKIFDTVII
310 320 330 340
HRQFYLFYNF EYWFLPMKPP FVKITYEETP LPTRHTTFTD L
Length:341
Mass (Da):39,329
Last modified:October 11, 2005 - v1
Checksum:i1969A4003CAF0E4D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 8PCG → TRP in AAH25487 (PubMed:15489334).Curated3
Sequence conflicti42R → H in BAC27797 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032293 mRNA. Translation: BAC27797.1.
AK144635 mRNA. Translation: BAE25980.1.
BC025487 mRNA. Translation: AAH25487.1.
CCDSiCCDS27314.1.
RefSeqiNP_001028414.1. NM_001033242.1.
UniGeneiMm.295289.

Genome annotation databases

EnsembliENSMUST00000022721; ENSMUSP00000022721; ENSMUSG00000022125.
GeneIDi211286.
KEGGimmu:211286.
UCSCiuc007uwg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK032293 mRNA. Translation: BAC27797.1.
AK144635 mRNA. Translation: BAE25980.1.
BC025487 mRNA. Translation: AAH25487.1.
CCDSiCCDS27314.1.
RefSeqiNP_001028414.1. NM_001033242.1.
UniGeneiMm.295289.

3D structure databases

ProteinModelPortaliQ3UMW8.
SMRiQ3UMW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000022721.

PTM databases

PhosphoSitePlusiQ3UMW8.

Proteomic databases

EPDiQ3UMW8.
MaxQBiQ3UMW8.
PaxDbiQ3UMW8.
PRIDEiQ3UMW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022721; ENSMUSP00000022721; ENSMUSG00000022125.
GeneIDi211286.
KEGGimmu:211286.
UCSCiuc007uwg.1. mouse.

Organism-specific databases

CTDi1203.
MGIiMGI:2442253. Cln5.

Phylogenomic databases

eggNOGiENOG410IJEH. Eukaryota.
ENOG4110RH0. LUCA.
GeneTreeiENSGT00390000010065.
HOGENOMiHOG000060233.
HOVERGENiHBG005345.
InParanoidiQ3UMW8.
KOiK12390.
OMAiWVKQDNE.
OrthoDBiEOG091G0B86.
PhylomeDBiQ3UMW8.
TreeFamiTF330864.

Miscellaneous databases

ChiTaRSiCln5. mouse.
PROiQ3UMW8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022125.
ExpressionAtlasiQ3UMW8. baseline and differential.
GenevisibleiQ3UMW8. MM.

Family and domain databases

InterProiIPR026138. CLN5.
[Graphical view]
PANTHERiPTHR15380. PTHR15380. 1 hit.
PfamiPF15014. CLN5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCLN5_MOUSE
AccessioniPrimary (citable) accession number: Q3UMW8
Secondary accession number(s): Q8C054, Q8R152
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 11, 2005
Last modified: November 30, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.